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公开(公告)号：US20200319193A1
公开(公告)日：2020-10-08
申请号：US16856619
申请日：2020-04-23
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US11740233B2
公开(公告)日：2023-08-29
申请号：US16014965
申请日：2018-06-21
发明人： Julia Ashworth-Sharpe , Christopher Bieniarz , Michael Farrell , Donald D. Johnson , Jerome W. Kosmeder , Adrian E. Murillo , Chol Steven Yun , Zhanna Zhilina
IPC分类号： G01N33/543 , G01N33/531 , G01N33/68 , G01N33/553 , C07F9/12 , C07F9/50 , C07F9/572 , C07F9/655 , G01N33/58
CPC分类号： G01N33/54346 , C07F9/12 , C07F9/5004 , C07F9/5022 , C07F9/5045 , C07F9/5728 , C07F9/65515 , C07F9/65517 , C07F9/65522 , G01N33/531 , G01N33/553 , G01N33/587 , G01N33/6854 , G01N2333/916
摘要： Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody-nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody-nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.
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公开(公告)号：US20200249238A1
公开(公告)日：2020-08-06
申请号：US16856589
申请日：2020-04-23
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US10031134B2
公开(公告)日：2018-07-24
申请号：US15263221
申请日：2016-09-12
发明人： Julia Ashworth-Sharpe , Chol Steven Yun , Zhanna Zhilina , Adrian E. Murillo , Donald D. Johnson , Michael Farrell , Jerome W. Kosmeder , Christopher Bieniarz
IPC分类号： G01N33/543 , G01N33/553
摘要： Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody-nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody-nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.
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公开(公告)号：US09618429B2
公开(公告)日：2017-04-11
申请号：US13744504
申请日：2013-01-18
IPC分类号： G01N1/30 , G01N33/532 , C12Q1/28 , G01N33/535
CPC分类号： G01N1/30 , C12Q1/28 , G01N33/532 , G01N33/535 , G01N33/54393 , G01N33/581 , G01N2001/302
摘要： Disclosed embodiments concern a composition comprising DAB chromogen, and/or derivative thereof, a stabilizer, and polymer capable of preventing or reducing DAB precipitation relative to a composition that does not comprise the polymer. Also disclosed herein is a method for using the disclosed composition and embodiments of a kit.
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公开(公告)号：US20170131271A1
公开(公告)日：2017-05-11
申请号：US15263221
申请日：2016-09-12
发明人： Julia Ashworth-Sharpe , Chol Steven Yun , Zhanna Zhilina , Adrian E. Murillo , Donald D. Johnson , Michael Farrell , Jerome W. Kosmeder , Christopher Bieniarz
IPC分类号： G01N33/543 , G01N33/553
CPC分类号： G01N33/54346 , C07F9/12 , C07F9/5004 , C07F9/5022 , C07F9/5045 , C07F9/5728 , C07F9/65515 , C07F9/65517 , C07F9/65522 , G01N33/531 , G01N33/553 , G01N33/587 , G01N33/6854 , G01N2333/916
摘要： Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody-nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody-nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.
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公开(公告)号：US20160195540A1
公开(公告)日：2016-07-07
申请号：US15052768
申请日：2016-02-24
发明人： Christopher Bieniarz , Jerome W. Kosmeder , Mark Lefever , Casey A. Kernag , Julia Ashworth-Sharpe
IPC分类号： G01N33/58 , C07K16/00 , C12N9/16 , C12N9/96 , C07C323/22
CPC分类号： G01N33/581 , A61K39/44 , A61K47/6801 , A61K47/6803 , A61K47/6815 , C07C319/02 , C07C323/22 , C07C323/60 , C07C327/28 , C07K16/00 , C12N9/16 , C12N9/96 , C12Y301/03001 , G01N33/532 , G01N33/535 , C07C323/12
摘要： A method is disclosed for making a conjugate of two molecules using a hydrazide thiol linker. In a particular working embodiment, an Fc-specific antibody-enzyme conjugate is made using the method and demonstrated to provide exceptional staining sensitivity and specificity in immunohistochemical and in situ hybridization assays.
摘要翻译： 公开了使用酰肼硫醇连接体制备两个分子的缀合物的方法。 在特定的工作实施方案中，使用该方法制备Fc特异性抗体 - 酶缀合物，并证实其在免疫组织化学和原位杂交测定中提供特殊的染色敏感性和特异性。
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公开(公告)号：US20200333349A1
公开(公告)日：2020-10-22
申请号：US16856604
申请日：2020-04-23
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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9.发明申请ANTIBODY-NANOPARTICLE CONJUGATES AND METHODS FOR MAKING AND USING SUCH CONJUGATES 有权抗体 - 纳米颗粒结合体和制备和使用这种结合体的方法公开(公告)号：US20160116462A1
公开(公告)日：2016-04-28
申请号：US14691826
申请日：2015-04-21
发明人： Julia Ashworth-Sharpe , Chol Steven Yun , Zhanna Zhilina , Adrian E. Murillo , Donald D. Johnson , Michael Farrell , Jerome W. Kosmeder , Christopher Bieniarz
IPC分类号： G01N33/531 , G01N33/58
CPC分类号： G01N33/54346 , C07F9/12 , C07F9/5004 , C07F9/5022 , C07F9/5045 , C07F9/5728 , C07F9/65515 , C07F9/65517 , C07F9/65522 , G01N33/531 , G01N33/553 , G01N33/587 , G01N33/6854 , G01N2333/916
摘要： Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody-nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody-nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.
摘要翻译： 本文公开了抗体 - 纳米颗粒缀合物，其包括直接连接到抗体或其片段的两个或更多个纳米颗粒（例如金，钯，铂，银，铜，镍，钴，铱或其两种或多种的合金） 金属硫醇键。 制备本文公开的抗体 - 纳米颗粒缀合物的方法包括使芳基膦 - 纳米颗粒复合物与还原抗体反应以产生抗体 - 纳米颗粒缀合物。 本文还公开了用于检测样品中靶分子的方法，其包括使用抗体 - 纳米颗粒缀合物（例如本文所述的抗体 - 纳米颗粒缀合物）和利用本文公开的方法检测靶分子的试剂盒。
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公开(公告)号：US09310373B2
公开(公告)日：2016-04-12
申请号：US14181391
申请日：2014-02-14
发明人： Christopher Bieniarz , Julia Ashworth-Sharpe , Casey A. Kernag , Jerome W. Kosmeder , Mark Lefever
IPC分类号： G01N33/535 , G01N33/58 , C07C319/02 , C07C323/60 , C07C327/28 , G01N33/532 , A61K47/48 , A61K39/44
CPC分类号： G01N33/581 , A61K39/44 , A61K47/6801 , A61K47/6803 , A61K47/6815 , C07C319/02 , C07C323/22 , C07C323/60 , C07C327/28 , C07K16/00 , C12N9/16 , C12N9/96 , C12Y301/03001 , G01N33/532 , G01N33/535 , C07C323/12
摘要： A method is disclosed for making a conjugate of two molecules using a hydrazide thiol linker. In a particular working embodiment, an Fc-specific antibody-enzyme conjugate is made using the method and demonstrated to provide exceptional staining sensitivity and specificity in immunohistochemical and in situ hybridization assays.
摘要翻译： 公开了使用酰肼硫醇连接体制备两个分子的缀合物的方法。 在特定的工作实施方案中，使用该方法制备Fc特异性抗体 - 酶缀合物，并证实其在免疫组织化学和原位杂交测定中提供特殊的染色敏感性和特异性。
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