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公开(公告)号：US20240210288A1
公开(公告)日：2024-06-27
申请号：US18599067
申请日：2024-03-07
发明人： Nelson Alexander , Aoune Barhoumi , Melinda Day , Lisa Gallegos , Katherine Leith , Samantha Rajkovich , Esteban Roberts , Stacey Stanislaw , Eric Walk
IPC分类号： G01N1/28 , A01N1/02 , G01N1/30 , G01N1/36 , G01N33/483
CPC分类号： G01N1/286 , A01N1/0226 , A01N1/0278 , G01N1/30 , G01N1/36 , G01N33/4833 , G01N2001/2866 , G01N2001/368
摘要： The disclosure generally relates to the preparation of representative samples from clinical samples, e.g., tumors (whole or in part), lymph nodes, metastases, cysts, polyps, or a combination or portion thereof, using mechanical and/or biochemical dissociation methods to homogenize intact samples or large portions thereof. The resulting homogenate provides the ability to obtain a correct representative sample despite spatial heterogeneity within the sample, increasing detection likelihood of low prevalence subclones, and is suitable for use in various diagnostic assays as well as the production of therapeutics, especially “personalized” anti-tumor vaccines or immune cell based therapies.
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公开(公告)号：US20200319193A1
公开(公告)日：2020-10-08
申请号：US16856619
申请日：2020-04-23
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US20180328932A1
公开(公告)日：2018-11-15
申请号：US16038389
申请日：2018-07-18
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , G01N33/542 , C12Q1/68 , G01N33/53 , C12Q1/682
CPC分类号： G01N33/581 , C12Q1/68 , C12Q1/682 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US20180320229A1
公开(公告)日：2018-11-08
申请号：US16034392
申请日：2018-07-13
IPC分类号： C12Q1/6874
CPC分类号： C12Q1/6874 , C12Q1/6869
摘要： In one aspect of the present disclosure is a targeted sequencing workflow where an input sample comprising a sufficient quantity of genomic material is provided such minimal or no amplification cycles are utilized prior to sequencing.
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公开(公告)号：US11906523B2
公开(公告)日：2024-02-20
申请号：US16856589
申请日：2020-04-23
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
CPC分类号： G01N33/581 , C12Q1/68 , C12Q1/682 , G01N33/53 , G01N33/542 , C07H21/02 , C07K14/00
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US20230250476A1
公开(公告)日：2023-08-10
申请号：US18131577
申请日：2023-04-06
IPC分类号： C12Q1/6874 , C12Q1/6869
CPC分类号： C12Q1/6874 , C12Q1/6869
摘要： In one aspect of the present disclosure is a targeted sequencing workflow where an input sample comprising a sufficient quantity of genomic material is provided such minimal or no amplification cycles are utilized prior to sequencing.
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公开(公告)号：US20200326345A1
公开(公告)日：2020-10-15
申请号：US16786647
申请日：2020-02-10
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US20190018018A1
公开(公告)日：2019-01-17
申请号：US16038374
申请日：2018-07-18
发明人： Nelson ALEXANDER , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , G01N33/542 , C12Q1/68 , G01N33/53 , C12Q1/682
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US11768137B2
公开(公告)日：2023-09-26
申请号：US16571785
申请日：2019-09-16
IPC分类号： G01N1/40 , B01L3/00 , C12Q1/6886 , G01N1/28 , G01N33/574
CPC分类号： G01N1/4077 , B01L3/502753 , B01L3/502761 , C12Q1/6886 , G01N1/286 , G01N33/57496 , B01L2200/0652 , B01L2300/0681 , B01L2400/0403 , B01L2400/086 , G01N2001/2866 , G01N2001/4088
摘要： The present disclosure provides a method of separating cellular particles from a tissue sample and then sorting the cellular particles into two or more cellular particle populations.
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公开(公告)号：US11603565B2
公开(公告)日：2023-03-14
申请号：US17252222
申请日：2019-06-14
发明人： Nelson R. Alexander , Aoune Barhoumi , Jan Berka , Rui Chen , Lisa L. Gallegos , Toumy Guettouche , Seoyoung Kim , Maeve E. O'Huallachain , Sedide Ozturk , Jigar Patel , Florian Rubelt , Stacey Stanislaw
IPC分类号： C12Q1/6883 , C12N15/10 , C12Q1/6806 , C12Q1/6881
摘要： The invention is a method of identifying a cognate antigen for a T-cell receptor using neoantigens from a patient's tumor cells combined with the patient's T-cells and using cell sorting, genome sequencing, expressing TCR genes, presenting tumor neoantigens on MHC complex and uniquely barcoding the T-cells where TCR recognition occurs to tag all components of the TCR recognition complex.
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