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    Automated RNA Detection Using Labeled 2'-O-Methyl RNA Oligonucleotide Probes and Signal Amplification Systems
    4.
    发明申请
    Automated RNA Detection Using Labeled 2'-O-Methyl RNA Oligonucleotide Probes and Signal Amplification Systems 审中-公开
    使用标记的2'-O-甲基RNA寡核苷酸探针和信号放大系统的自动RNA检测

    公开(公告)号：US20160376641A1

    公开(公告)日：2016-12-29

    申请号：US15245126

    申请日：2016-08-23

    申请人： Ventana Medical Systems, Inc.

    发明人： Michael Farrell ,  William Day ,  Zeyu Jiang ,  Anne Pedata

    IPC分类号： C12Q1/68

    摘要： Disclosed herein are methods and compositions for detecting differential expression of certain miRNAs in cancer cells or their surrounding normal tissues in the tumor microenvironment. The disclosure describes an automated, highly sensitive and specific method for detection of any cellular RNA molecule, including microRNA, messenger RNA and non-coding RNA. The technology includes probe design as well as probe use in an automated fashion for detection of RNA molecules in formalin-fixed paraffin-embedded tissue (FFPET) samples.

    摘要翻译： 本文公开了用于检测肿瘤微环境中癌细胞或其周围正常组织中某些miRNA的差异表达的方法和组合物。 本公开描述了用于检测任何细胞RNA分子（包括microRNA，信使RNA和非编码RNA）的自动化，高灵敏度和特异性的方法。 该技术包括探针设计以及以自动化方式探测在福尔马林固定石蜡包埋组织（FFPET）样品中检测RNA分子的探针。

    Method for producing nucleic acid probes
    5.
    发明授权
    Method for producing nucleic acid probes 有权
    生产核酸探针的方法

    公开(公告)号：US08828659B2

    公开(公告)日：2014-09-09

    申请号：US13792590

    申请日：2013-03-11

    申请人： Ventana Medical Systems, Inc.

    发明人： Michael Farrell

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6841 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q1/701 ,  C12Q1/708 ,  C12Q2600/112 ,  C12Q2600/156

    摘要： The present disclosure provides nucleic acid probes, as well as kits that include such probes. Methods for producing and using (for example in chromosomal in situ hybridization) the probes are also provided. Such probes in some examples are used to detect chromosomal abnormalities or the presence of a pathogen.

    摘要翻译： 本公开提供了核酸探针，以及包括这种探针的试剂盒。 还提供了用于生产和使用（例如染色体原位杂交）探针的方法。 一些实施例中的这种探针用于检测染色体异常或病原体的存在。

    POLYTAG PROBES
    9.
    发明申请
    POLYTAG PROBES 审中-公开
    POLYTAG探索

    公开(公告)号：US20170029877A1

    公开(公告)日：2017-02-02

    申请号：US15087443

    申请日：2016-03-31

    申请人： Ventana Medical Systems, Inc.

    发明人： Michael Farrell ,  Zeyu Jiang ,  William A. Day, JR.

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6841 ,  C12Q1/6816 ,  C12Q2563/131 ,  C12Q2565/1025 ,  C12Q2565/543

    摘要： The present invention provides probes and probe systems for detection of nucleic acids, and in particular probes and probe systems comprising target nucleic acid probes which comprise a plurality of detection sequences and detection nucleic acid probes which hybridize to the detection sequences of the target nucleic acid probes and which further comprise a plurality of detectable moieties, such as haptens.

    摘要翻译： 本发明提供用于检测核酸的探针和探针系统，特别是包含靶核酸探针的探针和探针系统，所述探针和探针系统包含多个检测序列和与靶核酸探针的检测序列杂交的检测核酸探针 并且其还包含多个可检测部分，例如半抗原。

    Proximity Assay for In Situ Detection of Targets
    10.
    发明申请
    Proximity Assay for In Situ Detection of Targets 有权
    目标检测目标接近检测

    公开(公告)号：US20160002701A1

    公开(公告)日：2016-01-07

    申请号：US14769892

    申请日：2014-03-11

    申请人： VENTANA MEDICAL SYSTEMS, INC.

    发明人： Michael Farrell ,  Rui Hong ,  Zeyu Jiang

    IPC分类号： C12Q1/25

    CPC分类号： C12Q1/25 ,  G01N33/535 ,  G01N33/581 ,  G01N2333/9015 ,  G01N2440/32

    摘要： A proximity detection method is described that utilizes enzymatic biotinylation to detect targets in a sample, particularly formalin fixed paraffin embedded samples using automated staining platforms. One disclosed embodiment comprises contacting the sample with a first conjugate comprising a biotin ligase and a first specific binding moiety that binds proximally to the first target; contacting the sample with a second conjugate comprising a biotin ligase substrate and a second specific binding moiety that binds proximally to the second target; subjecting the sample to conditions that allow biotinylation of the biotin ligase substrate by the biotin ligase when the first target and the second target have a proximal arrangement; and detecting biotinylation of the biotin ligase substrate. The conditions that allow biotinylation of the substrate include addition of biotin and ATP. The method also may comprise contacting the sample with a streptavidin-enzyme conjugate. Signal amplification also can be used.

    摘要翻译： 描述了利用酶生物素化来检测样品中的靶，特别是使用自动染色平台的福尔马林固定石蜡包埋样品的接近检测方法。 一个公开的实施方案包括使样品与包含生物素连接酶的第一缀合物和与第一靶近端结合的第一特异性结合部分接触; 使样品与包含生物素连接酶底物和第二特异性结合部分的第二缀合物接触，所述第二特异性结合部分与第二靶标近端结合; 当所述第一靶和所述第二靶具有近端排列时，使所述样品经受生物素连接酶使生物素连接酶底物生物素化的条件; 并检测生物素连接酶底物的生物素化。 允许底物生物素化的条件包括生物素和ATP的添加。 该方法还可以包括使样品与链霉亲和素 - 酶缀合物接触。 信号放大也可以使用。