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1.发明申请公开(公告)号：US20210341466A1
公开(公告)日：2021-11-04
申请号：US17318541
申请日：2021-05-12
IPC分类号： G01N33/533 , G01N33/58 , C09B23/08 , C09B11/24 , G01N33/532
摘要： Disclosed herein are novel chromogenic conjugates, the conjugates comprising at least two detectable moieties.
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2.发明授权公开(公告)号：US11092594B2
公开(公告)日：2021-08-17
申请号：US15665291
申请日：2017-07-31
发明人： Jerome W. Kosmeder, II , Mark Lefever , Donald Johnson , Michael Farrell , Zhanna Zhilina , Christopher Bieniarz
IPC分类号： G01N33/532 , C07D495/04 , G01N33/58 , G01N33/53 , G01N33/533 , C07D491/147 , C07D277/76 , C07D241/44 , C07D493/04 , C07D243/12 , C07D277/48 , C07D215/52 , C07D231/16 , C07D493/14 , C07D491/056 , C07D498/04 , C07D311/12 , C07D491/052 , C07D271/12 , C07D417/12 , C07D271/08 , C12Q1/6804 , C12Q1/6841 , C12Q1/6886
摘要： A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, athiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.
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公开(公告)号：US20210063411A1
公开(公告)日：2021-03-04
申请号：US17021659
申请日：2020-09-15
发明人： Christopher Bieniarz , Jan Froehlich , Brian Daniel Kelly , Rui Hong , Mark Lefever , Phillip Miller , Hong Wang
摘要： Particular disclosed embodiments disclosed herein concern using a one or more various mass tags, which can be specifically deposited at targets through direct or indirect enzymatic-catalyzed transformation, to provide a method for identifying targets in tissue samples. The mass tags may be labeled with stable isotopes to produce mass tags having the same chemical structure but different masses. Mass codes produced by ionizing the mass tags are detected and/or quantified using mass spectrometry. The method can be used for multiplexed detection of multiple targets in a particular sample. In some embodiments, a map divided into sections representing sections of the tissue sample may be prepared, with the map sections including data corresponding to quantification data wherein the size of a mass peak is determined and correlated with the amount of a target for the corresponding tissue sample section.
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公开(公告)号：US20200319193A1
公开(公告)日：2020-10-08
申请号：US16856619
申请日：2020-04-23
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US20180328932A1
公开(公告)日：2018-11-15
申请号：US16038389
申请日：2018-07-18
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , G01N33/542 , C12Q1/68 , G01N33/53 , C12Q1/682
CPC分类号： G01N33/581 , C12Q1/68 , C12Q1/682 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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6.发明授权公开(公告)号：US12130284B2
公开(公告)日：2024-10-29
申请号：US17318541
申请日：2021-05-12
IPC分类号： G01N33/533 , C09B11/24 , C09B23/08 , G01N33/532 , G01N33/58
CPC分类号： G01N33/533 , C09B11/24 , C09B23/083 , G01N33/532 , G01N33/582 , G01N33/583
摘要： Disclosed herein are novel chromogenic conjugates, the conjugates comprising at least two detectable moieties.
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7.发明公开公开(公告)号：US20240011099A1
公开(公告)日：2024-01-11
申请号：US18224446
申请日：2023-07-20
发明人： Daniel R. Bauer , Mark Lefever , Larry Morrison
IPC分类号： C12Q1/6886 , C12Q1/6841 , G01N33/574 , G01N1/30
CPC分类号： C12Q1/6886 , C12Q1/6841 , G01N33/57492 , G01N1/30 , G01N2001/302
摘要： The present disclosure is directed to a method of staining a biological specimen (e.g. a single serial tissue section derived from a biological sample) with one or more routine and/or special statins while concomitantly labeling the same biological specimen with one or more detectable moieties without the need for stripping any stain or evaluating different images of stained serial tissue sections of a biological specimen. In some embodiments, the present disclosure is directed to a biological specimen stained with one or more conventional dyes, and where the biological specimen further includes one or more biomarkers labeled with one or more detectable moieties.
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公开(公告)号：US20200249238A1
公开(公告)日：2020-08-06
申请号：US16856589
申请日：2020-04-23
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US09719986B2
公开(公告)日：2017-08-01
申请号：US14312341
申请日：2014-06-23
发明人： Jerome W. Kosmeder, II , Mark Lefever , Donald Johnson , Michael Farrell , Zhanna Zhilina , Christopher Bieniarz
IPC分类号： G01N33/53 , C07D495/04 , G01N33/532 , G01N33/533 , G01N33/58 , C12Q1/68
CPC分类号： G01N33/5308 , C07D495/04 , C12Q1/6804 , C12Q1/6841 , C12Q1/6886 , G01N33/532 , G01N33/533 , G01N33/58 , G01N33/582 , G01N33/583 , Y10S435/961 , Y10S530/807
摘要： A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.
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公开(公告)号：US20160139143A1
公开(公告)日：2016-05-19
申请号：US14981181
申请日：2015-12-28
发明人： Rui Hong , Hong Wang , Mark Lefever , Jan Froehlich , Christopher Bieniarz , Brian Daniel Kelly , Phillip Miller
CPC分类号： G01N33/6848 , G01N27/62
摘要： Particular disclosed embodiments disclosed herein concern using a one or more various mass tags, which can be specifically deposited at targets through direct or indirect enzymatic-catalyzed transformation, to provide a method for identifying targets in tissue samples. The mass tags may be labeled with stable isotopes to produce mass tags having the same chemical structure but different masses. Mass codes produced by ionizing the mass tags are detected and/or quantified using mass spectrometry. The method can be used for multiplexed detection of multiple targets in a particular sample. In some embodiments, a map divided into sections representing sections of the tissue sample may be prepared, with the map sections including data corresponding to quantification data wherein the size of a mass peak is determined and correlated with the amount of a target for the corresponding tissue sample section.
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