Genes coding for &bgr;-agarases and their use for producing agar biodegradation enzymes
    4.
    发明授权
    Genes coding for &bgr;-agarases and their use for producing agar biodegradation enzymes 有权
    编码β-琼脂糖的基因及其用于生产琼脂生物降解酶的用途

    公开(公告)号:US06511838B1

    公开(公告)日:2003-01-28

    申请号:US09719402

    申请日:2000-12-12

    IPC分类号: C12N900

    摘要: The invention relates to the novel Cytophaga drobachiensis strain deposited in the DSMZ Collection (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (German Collection of Microorganisms and Cell Cultures)) on May 8, 1998 under the number DSM 12170, the agaA gene coding for a &bgr;-agarase and having SEQ ID No. 1, the agaB gene coding for a &bgr;-agarase and having SEQ ID No. 3, said genes coding for a &bgr;-agarase of Cytophaga drobachiensis DSM 12170, and the specific nucleic acid sequence of the agaA gene coding for a specific peptide fragment AgaA′ which has conserved &bgr;-agarase activity, and having SEQ ID No. 5, as well as the protein AgaA of C. drobachiensis DSM 12170 having SEQ ID No. 2, the protein AgaB of C. drobachiensis DSM 12170 having SEQ ID No. 4 and said peptide fragment AgaA′ of C. drobachiensis DSM 12170 having SEQ ID No. 6.

    摘要翻译: 本发明涉及存在于DSMZ Collection(Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH(German Collection of Microorganisms and Cell Cultures))中的新颖的细胞托氏菌(Cytophaga drobachiensis),编号为DSM 12170,编码为β的agaA基因 - 具有SEQ ID No.1,编码β-琼脂糖酶并具有SEQ ID No.3的agaB基因的所述编码所述的编码Cytophaga drobachiensis DSM 12170的β-琼脂糖酶的基因,以及agaA的特异性核酸序列 编码具有保守的β-琼脂糖酶活性并具有SEQ ID No.5的特异性肽片段AgaA'的基因,以及具有SEQ ID No.2的C. drobachiensis DSM 12170的蛋白质AgaA,C的蛋白质AgaB。 具有SEQ ID No.4的drobachiensis DSM 12170和具有SEQ ID No.6的C. drobachiensis DSM 12170的所述肽片段AgaA'。