摘要:
The invention concerns endofucanases suitable for preparing fuco-oligosaccharides by enzymatic hydrolysis of fucanes. It also concerns a bacterium producing endofucanases and the preparation of said enzyme from the bacterium. It further concerns endofucanase genes and it finally concerns the use said fuco-oligosaccharides for plant protection.
摘要:
The present invention relates to genes which code for glycosyl hydrolases having an HCA score with the iota-carrageenase of Alteromonas fortis which is greater than or equal to 65% over the domain extending between amino acids 164 and 311 of the protein sequence SEQ ID No. 2 of said iota-carrageenase, and to genes which code for glycosyl hydrolases having an HCA score with the kappa-carrageenase of Alteromonas carrageenovora which is greater than or equal to 75% over the domain extending between amino acids 117 and 262 of the protein sequence SEQ ID No. 6 of said kappa-carrageenase.
摘要翻译:本发明涉及编码具有HCA评分的糖基水解酶的基因,其中所述糖基水解酶具有与所述蛋白质序列SEQ ID No.的氨基酸164和311之间延伸的结构域大于或等于65%的阿替单调杆菌的ι-角叉菜胶酶。 2的所述iota-角叉菜胶酶,以及编码具有HCA得分的糖基水解酶的基因,所述糖基水解酶具有与所述蛋白质序列的第117和262位氨基酸之间延伸的结构域大于或等于75%的卡拉胶单胞菌属的卡拉胶 所述κ-角叉菜胶酶的SEQ ID No.6。
摘要:
The present invention relates to genes which code for glycosyl hydrolases having an HCA score with the iota-carrageenase of Alteromonas fortis which is greater than or equal to 65% over the domain extending between amino acids 164 and 311 of the protein sequence SEQ ID No. 2 of said iota-carrageenase, and to genes which code for glycosyl hydrolases having an HCA score with the kappa-carrageenase of Alteromonas carrageenovora which is greater than or equal to 75% over the domain extending between amino acids 117 and 262 of the protein sequence SEQ ID No. 6 of said kappa-carrageenase.
摘要翻译:本发明涉及编码具有HCA评分的糖基水解酶的基因,其中所述糖基水解酶具有与所述蛋白质序列SEQ ID No.的氨基酸164和311之间延伸的结构域大于或等于65%的阿替单调杆菌的ι-角叉菜胶酶。 2的所述iota-角叉菜胶酶,以及编码具有HCA得分的糖基水解酶的基因,所述糖基水解酶具有与所述蛋白质序列的第117和262位氨基酸之间延伸的结构域大于或等于75%的卡拉胶单胞菌属的卡拉胶 所述κ-角叉菜胶酶的SEQ ID No.6。
摘要:
The invention relates to the novel Cytophaga drobachiensis strain deposited in the DSMZ Collection (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (German Collection of Microorganisms and Cell Cultures)) on May 8, 1998 under the number DSM 12170, the agaA gene coding for a &bgr;-agarase and having SEQ ID No. 1, the agaB gene coding for a &bgr;-agarase and having SEQ ID No. 3, said genes coding for a &bgr;-agarase of Cytophaga drobachiensis DSM 12170, and the specific nucleic acid sequence of the agaA gene coding for a specific peptide fragment AgaA′ which has conserved &bgr;-agarase activity, and having SEQ ID No. 5, as well as the protein AgaA of C. drobachiensis DSM 12170 having SEQ ID No. 2, the protein AgaB of C. drobachiensis DSM 12170 having SEQ ID No. 4 and said peptide fragment AgaA′ of C. drobachiensis DSM 12170 having SEQ ID No. 6.
摘要翻译:本发明涉及存在于DSMZ Collection(Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH(German Collection of Microorganisms and Cell Cultures))中的新颖的细胞托氏菌(Cytophaga drobachiensis),编号为DSM 12170,编码为β的agaA基因 - 具有SEQ ID No.1,编码β-琼脂糖酶并具有SEQ ID No.3的agaB基因的所述编码所述的编码Cytophaga drobachiensis DSM 12170的β-琼脂糖酶的基因,以及agaA的特异性核酸序列 编码具有保守的β-琼脂糖酶活性并具有SEQ ID No.5的特异性肽片段AgaA'的基因,以及具有SEQ ID No.2的C. drobachiensis DSM 12170的蛋白质AgaA,C的蛋白质AgaB。 具有SEQ ID No.4的drobachiensis DSM 12170和具有SEQ ID No.6的C. drobachiensis DSM 12170的所述肽片段AgaA'。
摘要:
The present invention relates to the isolation and to the characterization of two proteins having a novel enzymatic activity, i.e. a porphyranase activity. These proteins are useful for hydrolyzing polysaccharides containing sulfated agaro-colloids and for producing oligo-porphyrans, notably oligo-porphyrans with a defined structure and size.
摘要:
The present invention relates to the isolation and to the characterization of two proteins having a novel enzymatic activity, i.e. a porphyranase activity. These proteins are useful for hydrolyzing polysaccharides containing sulfated agaro-colloids and for producing oligo-porphyrans, notably oligo-porphyrans with a defined structure and size.