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    CRYSTAL STRUCTURE OF GLYPHOSATE ACETYLTRANSFERASE (GLYAT) AND METHODS OF USE
    6.
    发明申请
    CRYSTAL STRUCTURE OF GLYPHOSATE ACETYLTRANSFERASE (GLYAT) AND METHODS OF USE 审中-公开
    谷氨酸乙酰胆碱酯酶（GLYAT）的晶体结构及其使用方法

    公开(公告)号：US20120288914A1

    公开(公告)日：2012-11-15

    申请号：US13382780

    申请日：2010-07-07

    申请人： Daniel Siehl ,  Robert J. Keenan ,  Zhenglin Hou ,  Linda A. Castle

    发明人： Daniel Siehl ,  Robert J. Keenan ,  Zhenglin Hou ,  Linda A. Castle

    IPC分类号： C12N9/10 ,  G06F19/16

    CPC分类号： C12N9/1029 ,  C07K2299/00

    摘要： The presently disclosed subject matter provides compositions and methods for evaluating the potential of candidate polypeptides to associate with glyphosate with a higher binding affinity, higher binding specificity, or both or to have N-acetyltransferase activity with a higher catalytic rate when compared to a native glyphosate acetyltransferase (GLYAT) polypeptide through the provision and comparison of three-dimensional molecular structures of the candidate polypeptides and the GLYAT polypeptides provided herein. The methods further provide for identification of polypeptides with these advantageous properties using the three-dimensional molecular structures of GLYAT polypeptides.

    摘要翻译： 目前公开的主题提供了组合物和方法，用于与天然草甘膦相比，评估候选多肽与具有更高结合亲和力，更高结合特异性或两者的草甘膦缔合或具有较高催化速率的N-乙酰转移酶活性的潜在性 乙酰转移酶（GLYAT）多肽，通过提供和比较本文提供的候选多肽和GLYAT多肽的三维分子结构。 所述方法进一步提供了使用GLYAT多肽的三维分子结构鉴定具有这些有利性质的多肽。

    METHODS AND COMPOSITIONS FOR THE MODULATION OF AMINO ACID BIOSYNTHESIS
    7.
    发明申请
    METHODS AND COMPOSITIONS FOR THE MODULATION OF AMINO ACID BIOSYNTHESIS 审中-公开
    用于调节氨基酸生物合成的方法和组合物

    公开(公告)号：US20100115661A1

    公开(公告)日：2010-05-06

    申请号：US12581331

    申请日：2009-10-19

    申请人： John H. Duesing ,  Anthony J. Kinney ,  Daniel Siehl

    发明人： John H. Duesing ,  Anthony J. Kinney ,  Daniel Siehl

    IPC分类号： C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8243 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254

    摘要： Compositions and methods for increasing the level of one or more selected free ε and/or α-N-acetylated amino acids in a selected tissue or organ of a plant are provided. In specific embodiments, the plant, plant part, seed or grain comprises a free α-N-acetylated amino acid content of at least 200 ppm or a ratio of free α-N-acetylated amino acid content to free non-α-N-acetylated amino acid content of about 3 to about 1000. Compositions comprising plants, plant parts, seed and grain having stably incorporated into their genome a heterologous polynucleotide encoding an amino acid-N-acetyltransferase polypeptide operably linked to a promoter active in the seed are provided. Further provided are compositions comprising a plant, plant part, seed or grain having stably incorporated into their genome a first heterologous polynucleotide encoding a first amino acid-N-acetyltransferase polypeptide operably linked to a first promoter active in the seed and a second heterologous polynucleotide encoding a second amino acid-N-acetyltransferase polypeptide operably linked to a second promoter active in the seed, wherein the first and the second amino acid-N-acetyltransferase polypeptide acetylate the α-amine of distinct amino acids. Compositions comprising food sources, feed and supplements, along with methods of increasing the nutritional value of a plant, plant part, seed or grain, are further provided.

    摘要翻译： 用于增加一个或多个所选择的免费等级的组合和方法 和/或α-N-乙酰化氨基酸在植物的选定组织或器官中。 在具体实施方案中，植物，植物部分，种子或颗粒包含至少200ppm的游离α-N-乙酰化氨基酸含量或游离的α-N-乙酰化氨基酸含量与游离的非α-N- 提供约3至约1000的乙酰化氨基酸含量。提供包含植物，植物部分，种子和颗粒的组合物，其稳定地并入其基因组中，编码与种子中活性物质活化的启动子可操作地连接的氨基酸-N-乙酰转移酶多肽的异源多核苷酸 。 进一步提供的是包含植物，植物部分，种子或谷物的组合物，其稳定地并入其基因组中，第一异源多核苷酸编码与种子中有活性的第一启动子可操作地连接的第一氨基酸-N-乙酰转移酶多肽和第二异源多核苷酸编码 可操作地连接到在种子中有活性的第二启动子的第二氨基酸-N-乙酰转移酶多肽，其中所述第一和第二氨基酸-N-乙酰转移酶多肽对不同氨基酸的α-胺进行乙酰化。 还提供了包含食物来源，饲料和补充剂的组合物，以及增加植物，植物部分，种子或谷物的营养价值的方法。