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公开(公告)号：US09719986B2
公开(公告)日：2017-08-01
申请号：US14312341
申请日：2014-06-23
发明人： Jerome W. Kosmeder, II , Mark Lefever , Donald Johnson , Michael Farrell , Zhanna Zhilina , Christopher Bieniarz
IPC分类号： G01N33/53 , C07D495/04 , G01N33/532 , G01N33/533 , G01N33/58 , C12Q1/68
CPC分类号： G01N33/5308 , C07D495/04 , C12Q1/6804 , C12Q1/6841 , C12Q1/6886 , G01N33/532 , G01N33/533 , G01N33/58 , G01N33/582 , G01N33/583 , Y10S435/961 , Y10S530/807
摘要： A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.
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公开(公告)号：US09017954B2
公开(公告)日：2015-04-28
申请号：US14259947
申请日：2014-04-23
IPC分类号： G01N33/53 , G01N33/532 , B01L3/00 , C12Q1/68 , A61K47/48 , A61K49/00 , G01N33/543 , G01N33/58 , C07K17/08 , G01N33/544 , G01N33/545 , G01N33/569 , G01N33/574
CPC分类号： G01N33/581 , A61K47/6883 , A61K49/0058 , A61K49/0067 , C07K17/08 , G01N33/532 , G01N33/54353 , G01N33/544 , G01N33/545 , G01N33/56983 , G01N33/57492 , G01N33/582 , G01N33/587 , G01N33/588
摘要： Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.
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3.发明授权公开(公告)号：US11092594B2
公开(公告)日：2021-08-17
申请号：US15665291
申请日：2017-07-31
发明人： Jerome W. Kosmeder, II , Mark Lefever , Donald Johnson , Michael Farrell , Zhanna Zhilina , Christopher Bieniarz
IPC分类号： G01N33/532 , C07D495/04 , G01N33/58 , G01N33/53 , G01N33/533 , C07D491/147 , C07D277/76 , C07D241/44 , C07D493/04 , C07D243/12 , C07D277/48 , C07D215/52 , C07D231/16 , C07D493/14 , C07D491/056 , C07D498/04 , C07D311/12 , C07D491/052 , C07D271/12 , C07D417/12 , C07D271/08 , C12Q1/6804 , C12Q1/6841 , C12Q1/6886
摘要： A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, athiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.
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公开(公告)号：US20210002726A1
公开(公告)日：2021-01-07
申请号：US16879228
申请日：2020-05-20
发明人： Michael Farrell , Antony Hubbard , Donald Johnson , Brian Kelly , Taylor Shingler , Lei Tang , Wenjun Zhang
IPC分类号： C12Q1/6886 , C12Q1/6841
摘要： Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization.
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公开(公告)号：US20150309039A1
公开(公告)日：2015-10-29
申请号：US14789077
申请日：2015-07-01
CPC分类号： G01N33/581 , A61K47/6883 , A61K49/0058 , A61K49/0067 , C07K17/08 , G01N33/532 , G01N33/54353 , G01N33/544 , G01N33/545 , G01N33/56983 , G01N33/57492 , G01N33/582 , G01N33/587 , G01N33/588
摘要： Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.
摘要翻译： 本发明的某些公开的实施方案涉及到具有多个与多个感兴趣的分子反应的反应性官能团的离散的，较短的聚合物的合成，衍生化，免疫球蛋白的缀合和信号扩增。 反应性官能团，例如酰肼，可用各种可检测的标记，特别是半抗原衍生化。 剩余的反应性官能团可以直接与特异性结合分子结合，例如与抗体Fc区的氧化碳水化合物结合。 与基于用单个半抗原衍生的分子相比，所公开的缀合物显示出大的信号扩增，并且可用于测定方法，特别是多重测定。
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6.发明申请公开(公告)号：US20140243236A1
公开(公告)日：2014-08-28
申请号：US14259947
申请日：2014-04-23
IPC分类号： G01N33/545 , G01N33/544 , G01N33/569 , C07K17/08 , G01N33/574
CPC分类号： G01N33/581 , A61K47/6883 , A61K49/0058 , A61K49/0067 , C07K17/08 , G01N33/532 , G01N33/54353 , G01N33/544 , G01N33/545 , G01N33/56983 , G01N33/57492 , G01N33/582 , G01N33/587 , G01N33/588
摘要： Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.
摘要翻译： 本发明的某些公开的实施方案涉及到具有多个与多个感兴趣的分子反应的反应性官能团的离散的，较短的聚合物的合成，衍生化，免疫球蛋白的缀合和信号扩增。 反应性官能团，例如酰肼，可用各种可检测的标记，特别是半抗原衍生化。 剩余的反应性官能团可以直接与特异性结合分子结合，例如与抗体Fc区的氧化碳水化合物结合。 与基于用单个半抗原衍生的分子相比，所公开的缀合物显示出大的信号扩增，并且可用于测定方法，特别是多重测定。
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公开(公告)号：US09103822B2
公开(公告)日：2015-08-11
申请号：US13905918
申请日：2013-05-30
IPC分类号： G01N33/542 , C07K16/00 , G01N33/532 , A61K47/48 , A61K49/00 , G01N33/543 , G01N33/58 , C07K17/08 , G01N33/544 , G01N33/545 , G01N33/569 , G01N33/574 , C12Q1/68 , G01N33/566
CPC分类号： G01N33/581 , A61K47/6883 , A61K49/0058 , A61K49/0067 , C07K17/08 , G01N33/532 , G01N33/54353 , G01N33/544 , G01N33/545 , G01N33/56983 , G01N33/57492 , G01N33/582 , G01N33/587 , G01N33/588
摘要： Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.
摘要翻译： 本发明的某些公开的实施方案涉及到具有多个与多个感兴趣的分子反应的反应性官能团的离散的，较短的聚合物的合成，衍生化，免疫球蛋白的缀合和信号扩增。 反应性官能团，例如酰肼，可用各种可检测的标记，特别是半抗原衍生化。 剩余的反应性官能团可以直接与特异性结合分子结合，例如与抗体Fc区的氧化碳水化合物结合。 与基于用单个半抗原衍生的分子相比，所公开的缀合物显示出大的信号扩增，并且可用于测定方法，特别是多重测定。
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公开(公告)号：US09575067B2
公开(公告)日：2017-02-21
申请号：US14789077
申请日：2015-07-01
IPC分类号： G01N33/53 , C07H19/04 , G01N33/58 , A61K47/48 , A61K49/00 , G01N33/532 , G01N33/543 , C07K17/08 , G01N33/544 , G01N33/545 , G01N33/569 , G01N33/574
CPC分类号： G01N33/581 , A61K47/6883 , A61K49/0058 , A61K49/0067 , C07K17/08 , G01N33/532 , G01N33/54353 , G01N33/544 , G01N33/545 , G01N33/56983 , G01N33/57492 , G01N33/582 , G01N33/587 , G01N33/588
摘要： Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.
摘要翻译： 本发明的某些公开的实施方案涉及到具有多个与多个感兴趣的分子反应的反应性官能团的离散的，较短的聚合物的合成，衍生化，免疫球蛋白的缀合和信号扩增。 反应性官能团，例如酰肼，可用各种可检测的标记，特别是半抗原衍生化。 剩余的反应性官能团可以直接与特异性结合分子结合，例如与抗体Fc区的氧化碳水化合物结合。 与基于用单个半抗原衍生的分子相比，所公开的缀合物显示出大的信号扩增，并且可用于测定方法，特别是多重测定。
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9.发明申请Single-Stranded Oligonucleotide Probes for Chromosome or Gene Copy Enumeration 审中-公开用于染色体或基因复制计数的单链寡核苷酸探针公开(公告)号：US20170009305A1
公开(公告)日：2017-01-12
申请号：US15242471
申请日：2016-08-19
发明人： Michael Farrell , Antony Hubbard , Donald Johnson , Brian Kelly , Taylor Shingler , Lei Tang , Wenjun Zhang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6841 , C12Q2600/156 , C12Q2600/158 , C12Q2537/143 , C12Q2537/16 , C12Q2537/164
摘要： Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization.
摘要翻译： 用于染色体计数，基因拷贝计数或组织诊断的单链寡核苷酸探针，系统，试剂盒和方法。 探针特别适用于单次，双重或多重测定中检测组织样品中的基因扩增，缺失或重排。 与行业领先的双链探针相比，探针表现出改善的性能; 特别是在杂交速率方面。
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公开(公告)号：US10752955B2
公开(公告)日：2020-08-25
申请号：US15242471
申请日：2016-08-19
发明人： Michael Farrell , Antony Hubbard , Donald Johnson , Brian Kelly , Taylor Shingler , Lei Tang , Wenjun Zhang
IPC分类号： C12Q1/68 , C12Q1/6886 , C12Q1/6841
摘要： Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization.
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