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公开(公告)号:US20130156801A1
公开(公告)日:2013-06-20
申请号:US13327244
申请日:2011-12-15
申请人: Vincent Craig BOND , Michael POWELL , Ming Bo HUANG , Syed ALI
发明人: Vincent Craig BOND , Michael POWELL , Ming Bo HUANG , Syed ALI
IPC分类号: A61K39/21 , C12N5/071 , A61P37/04 , A61K39/00 , G01N33/567 , C07K14/155 , C12P21/00 , A61K38/16
CPC分类号: C07K14/005 , A61K9/127 , A61K38/00 , A61K38/162 , A61K39/39 , A61K45/06 , A61K2039/55555 , C07K14/163 , C07K2319/055 , C07K2319/50 , C07K2319/60 , C12N15/00 , C12N2740/16322 , C12P21/02 , G01N33/543 , G01N33/566
摘要: The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.
摘要翻译: 本申请涉及生产外来体的方法。 本申请还提供了一种制备蛋白质组合物的方法,包括培养表达融合蛋白质的Nef-融合蛋白的外来体产生细胞,所述Nef-融合蛋白包含与目的蛋白融合的Nef衍生肽; 从外来体产生细胞培养物中分离外来体; 并从分离的外来体纯化目的蛋白质。 本申请还公开了包含含有Nef-融合蛋白的外来体的组合物,以及使用Nef-融合蛋白和含有Nef-融合蛋白的外来体的方法。