公开(公告)号：US20100304445A1

公开(公告)日：2010-12-02

申请号：US12664899

申请日：2008-06-16

申请人： Waclaw Szybalski ,  Frederick Blattner ,  David Frisch ,  Douglas Wieczorek

发明人： Waclaw Szybalski ,  Frederick Blattner ,  David Frisch ,  Douglas Wieczorek

IPC分类号： C12N15/64 ,  C12P19/34

CPC分类号： C07H1/00 ,  C07H5/10 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2565/537 ,  C12Q2565/515 ,  C12Q2535/125

摘要： The present invention relates to cloning target nucleic acids using phage packaging mechanisms. Packaging initiation sites may be introduced into the target DNA. Components of a phage packaging system may be combined with the target DNA to package the DNA into phage capsids. The packaged DNA may be used to create a library of target nucleic acids, or it may be sequenced.

摘要翻译： 本发明涉及使用噬菌体包装机制克隆靶核酸。 可以将包装起始位点引入目标DNA。 噬菌体包装系统的组分可以与目标DNA组合以将DNA包装到噬菌体衣壳中。 包装的DNA可用于产生靶核酸文库，或者可以测序。

公开(公告)号：US20100144548A1

公开(公告)日：2010-06-10

申请号：US12375911

申请日：2007-08-03

申请人： Waclaw Szybalski ,  Jadwiga Wild ,  Zdenka Hradecna ,  David Frisch ,  Frederick R. Blattner ,  Katarzyna Gromek

发明人： Waclaw Szybalski ,  Jadwiga Wild ,  Zdenka Hradecna ,  David Frisch ,  Frederick R. Blattner ,  Katarzyna Gromek

IPC分类号： C40B40/02 ,  C12N15/74 ,  C12N1/21 ,  C12N15/64 ,  C40B50/06 ,  C12N5/071

CPC分类号： C12N15/70 ,  C12N15/1093 ,  C12N15/64 ,  C12N15/66

摘要： The present invention relates generally to the field of molecular biology and genomics. More specifically, the present invention concerns the cloning of nucleic acid molecules and the production of nucleic acid libraries, as well as the expression of recombinant proteins and bactofection.

摘要翻译： 本发明一般涉及分子生物学和基因组学领域。 更具体地，本发明涉及核酸分子的克隆和核酸文库的产生，以及重组蛋白质和细菌转染的表达。

公开(公告)号：US06864087B2

公开(公告)日：2005-03-08

申请号：US10209792

申请日：2002-07-31

申请人： Waclaw Szybalski ,  Jadwiga Wild ,  Zdenka Hradecna

发明人： Waclaw Szybalski ,  Jadwiga Wild ,  Zdenka Hradecna

IPC分类号： C12N15/63 ,  C12N15/69 ,  C12N15/70

CPC分类号： C12N15/63

摘要： A single-copy BAC vector (containing or lacking an insert) is converted in a host cell into a conditional high-copy BAC vector by introducing a conditional origin of replication into the single-copy BAC vector. The conditional ori is introduced by site-specific recombination between the SC BAC vector and a vector that contains the conditional ori. The host cell comprises a recombinase that recognizes a site-specific recombination site on both the BAC vector and the conditional ori vector. In the presence of the recombinase, the conditional ori-containing vector recombines into the BAC vector to produce a high-copy BAC vector that can be conditionally amplified by activating the conditional origin of replication on command.

摘要翻译： 通过将有条件的复制起点引入到单拷贝BAC载体中，将单个副BAC载体（包含或缺少插入片段）在宿主细胞中转化为条件高拷贝BAC载体。 有条件的ori是通过SC BAC载体与含有条件的ori的载体之间的位点特异性重组引入的。 宿主细胞包含识别BAC载体和条件性ori载体上的位点特异性重组位点的重组酶。 在存在重组酶的情况下，将含条件的载体载体重组到BAC载体中以产生可通过在命令上激活条件复制起点而有条件地扩增的高拷贝BAC载体。

公开(公告)号：US20050214838A1

公开(公告)日：2005-09-29

申请号：US11077530

申请日：2005-03-10

申请人： Waclaw Szybalski ,  Alfredo Villasante ,  Jadwiga Wild ,  Maria Mendez-Lago

发明人： Waclaw Szybalski ,  Alfredo Villasante ,  Jadwiga Wild ,  Maria Mendez-Lago

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874

摘要： The present invention relates to methods and constructs for sequencing, mapping and ordering polynucleotide sequences. The invention finds particular applicability in analysis of repetitive DNA sequences such as heterochromatic sequences.

摘要翻译： 本发明涉及用于测序，测序和排序多核苷酸序列的方法和构建体。 本发明特别适用于分析重复DNA序列，如异染色序列。

公开(公告)号：US5874259A

公开(公告)日：1999-02-23

申请号：US975763

申请日：1997-11-21

申请人： Waclaw Szybalski

发明人： Waclaw Szybalski

IPC分类号： C12N15/70 ,  C12P19/34

CPC分类号： C12N15/70

摘要： A system for obtaining large amounts of a genomic DNA fragment from a bacterial artificial chromosome includes a vector that has a site into which the genomic fragment can be cloned and, flanking the site are excision mediating sites. The vector also includes, between the excision mediating sites and near one of the sites, a controllable origin of replication.

摘要翻译： 用于从细菌人造染色体获得大量基因组DNA片段的系统包括具有可以克隆基因组片段的位点的载体，并且位点侧翼是切除介导位点。 载体还包括在切除介导位点和近一个位点之间的可控起始复制。

公开(公告)号：US4935357A

公开(公告)日：1990-06-19

申请号：US826220

申请日：1986-02-05

申请人： Waclaw Szybalski

发明人： Waclaw Szybalski

IPC分类号： C12N15/09 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/00 ,  C12N15/10

CPC分类号： C12N9/22 ,  C12N15/10 ,  C12N9/96

摘要： A universal restriction endonuclease for cleaving a target DNA at a predetermined site. The universal restriction endonuclease utilizes a tailored oligodeoxynucleotide adapter in conjunction with a restriction enzyme which cuts DNA at a known distance from its recognition site. The oligodeoxynucleotide adapter mimics the relationship which exists in such enzymes between its recognition site and cut site.

摘要翻译： 用于在预定位点切割靶DNA的通用限制性内切核酸酶。 通用限制性内切核酸酶利用定制的寡脱氧核苷酸衔接子与限制性内切酶结合，该限制酶在与其识别位点已知距离处切割DNA。 寡脱氧核苷酸适配器模拟这种酶在其识别位点和切割位点之间存在的关系。