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    Solid support reagents for the direct synthesis of 3'-labeled polynucleotides
    3.
    发明授权
    Solid support reagents for the direct synthesis of 3'-labeled polynucleotides 失效
    用于直接合成3'-标记的多核苷酸的固体支持试剂

    公开(公告)号：US5736626A

    公开(公告)日：1998-04-07

    申请号：US593031

    申请日：1996-01-29

    申请人： Khairuzzaman Bashar Mullah ,  William A. Andrus

    发明人： Khairuzzaman Bashar Mullah ,  William A. Andrus

    IPC分类号： G01N33/58 ,  C07H1/00 ,  C07H21/00

    CPC分类号： C07H21/00 ,  Y02P20/55 ,  Y10S435/81

    摘要： The compounds of the invention are exemplified by the class of diglycolate synthesis supports particularly useful as support reagents for the direct synthesis of 3'-labeled polynucleotides. Generally, the compounds of the invention have the structure ##STR1## where T is an acid-cleavable hydroxyl protecting group, e.g., 4,4'-dimethoxytrityl; L.sub.1 is a linker for connecting a 3'-terminal nitrogen to carbon; L.sub.2 and L.sub.3 are linkers for connecting oxygen and carbon; W is a solid support, e.g., CPG or polystyrene; L.sub.4 is a linker for connecting the solid support to nitrogen; R.sub.1 and R.sub.2 are nitrogen substituents, e.g., hydrogen, lower alkyl, nitrogen protecting group, or label; and R.sub.3 through R.sub.7 are carbon substituents, e.g., hydrogen or lower alkyl. In a first particularly preferred embodiment, the synthesis supports of the invention are exemplified by compounds having the structure ##STR2## where DMT is 4,4'-dimethoxytrityl and W is polystyrene. In a second particularly preferred embodiment, the synthesis supports of the invention are exemplified by compounds having the structure ##STR3## where DMT and W are defined above.

    摘要翻译： 本发明的化合物的例子是特别可用作直接合成3'-标记的多核苷酸的载体试剂的二
    乙酸酯合成载体。 通常，本发明的化合物具有结构保护基，例如4,4'-二甲氧基三苯甲基; L1是用于连接3'-末端氮与碳的连接体; L2和L3是用于连接氧和碳的接头; W是固体载体，例如CPG或聚苯乙烯; L4是用于将固体载体连接到氮的接头; R1和R2是氮取代基，例如氢，低级烷基，氮保护基或标记; 并且R 3至R 7为碳取代基，例如氢或低级烷基。 在第一个特别优选的实施方案中，本发明的合成载体的例子是具有结构“IMAGE”的化合物，其中DMT是4,4'-二甲氧基三苯甲基，W是聚苯乙烯。 在第二特别优选的实施方案中，本发明的合成载体的例子是具有上述结构的化合物，其中DMT和W的定义如上。

    Automated system for polynucleotide synthesis and purification
    4.
    发明授权
    Automated system for polynucleotide synthesis and purification 失效
    用于多核苷酸合成和纯化的自动化系统

    公开(公告)号：US06175006B1

    公开(公告)日：2001-01-16

    申请号：US09281205

    申请日：1999-03-30

    申请人： William A. Andrus ,  Christie D. McCollum ,  Gerald Zon

    发明人： William A. Andrus ,  Christie D. McCollum ,  Gerald Zon

    IPC分类号： C07H2100

    CPC分类号： C07H21/00 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00351 ,  B01J2219/00389 ,  B01J2219/00454 ,  B01J2219/005 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/0068 ,  B01J2219/00686 ,  B01J2219/00722 ,  C12N15/1006 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14

    摘要： A method and system for polynucleotide synthesis are provided which employ solid phase synthesis on a nonswellable porous polystyrene support by phosphoramidite or hydrogen phosphonate chemistries. The polystyrene support gives rise to fewer tritylated failure sequences caused by chain growth from extraneous support sites, and allows lower amounts of monomer reactants to be used to achieve equal or better coupling efficiencies as those achieveable with CPG. The method and system also employ nucleoside intermediates whose exocyclic amines are protected by base-labile groups which permit simultaneous cleavage and deprotection of the completed polynucleotide chain in the presence of the solid phase support. This latter feature allows practical automation of both the synthesis and purification of polynucleotides.

    摘要翻译： 提供了一种用于多核苷酸合成的方法和系统，其通过亚磷酰胺或氢化膦酸盐化学物质在不可分散的多孔聚苯乙烯载体上进行固相合成。 聚苯乙烯支持物产生由来自外部支持位点的链增长引起的三苯甲基化失败序列的更少，并且允许使用较少量的单体反应物来实现与CPG可达到的相同或更好的偶联效率。 该方法和系统还使用核苷中间体，其外环胺由碱不稳定基团保护，其允许在固相载体存在下同时裂解和脱保护完整的多核苷酸链。 后一个特征允许多核苷酸的合成和纯化的实际自动化。