公开(公告)号：US20050074840A1

公开(公告)日：2005-04-07

申请号：US10496218

申请日：2004-09-13

Applicant: William Brondyk ,  Xuliang Jiang ,  Rene Schweickhardt

Inventor： William Brondyk ,  Xuliang Jiang ,  Rene Schweickhardt

IPC: C12N15/09 ,  C07K14/47 ,  C07K14/715 ,  C12N15/00 ,  C12P21/02 ,  C07H21/04

CPC classification number: C12P21/02 ,  C07K14/7151 ,  C12N15/00 ,  Y10S930/144

Abstract: Abstract of the DisclosureThe present invention relates to methods of increasing protein expression levels whereby at least one amino acid in a protein amino acid sequence is substituted for the amino acid, proline. Preferably, the substitution occurs within 15 amino acids, more preferably within 10 amino acids and most preferably within 5 amino acids of a cysteine amino acid residue. The present invention not only includes methods for polypeptides with proline substitutions, but also polynucleotides with codon substitutions for which a codon for any amino acid, except proline, is substituted for a codon encoding for proline.

Abstract translation: 发明内容本发明涉及增加蛋白质表达水平的方法，其中蛋白质氨基酸序列中的至少一个氨基酸被氨基酸脯氨酸取代。 优选地，取代发生在半胱氨酸氨基酸残基的15个氨基酸内，更优选在10个氨基酸内，最优选在5个氨基酸内。 本发明不仅包括具有脯氨酸取代的多肽的方法，而且还包括具有密码子取代的多核苷酸，除了脯氨酸之外，任何氨基酸的密码子都代替编码脯氨酸的密码子。

公开(公告)号：US20080014635A1

公开(公告)日：2008-01-17

申请号：US11778593

申请日：2007-07-16

Applicant: William Brondyk ,  Xuliang Jiang ,  Rene Schweickhardt

Inventor： William Brondyk ,  Xuliang Jiang ,  Rene Schweickhardt

IPC: C12N5/00 ,  C07H21/00 ,  C12N1/16 ,  C12N15/63

CPC classification number: C12P21/02 ,  C07K14/7151 ,  C12N15/00 ,  Y10S930/144

Abstract: The present invention relates to methods of increasing protein expression levels whereby at least one amino acid in a protein amino acid sequence is substituted for the amino acid, proline. Preferably, the substitution occurs within 15 amino acids, more preferably within 10 amino acids and most preferably within 5 amino acids of a cysteine amino acid residue. The present invention not only includes methods for polypeptides with proline substitutions, but also polynucleotides with codon substitutions for which a codon for any amino acid, except proline, is substituted for a codon encoding for proline.

Abstract translation: 本发明涉及增加蛋白质表达水平的方法，其中蛋白质氨基酸序列中的至少一个氨基酸被氨基酸脯氨酸取代。 优选地，取代发生在半胱氨酸氨基酸残基的15个氨基酸内，更优选在10个氨基酸内，最优选在5个氨基酸内。 本发明不仅包括具有脯氨酸取代的多肽的方法，而且还包括具有密码子取代的多核苷酸，除了脯氨酸之外，任何氨基酸的密码子都代替编码脯氨酸的密码子。