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公开(公告)号:US20050074840A1
公开(公告)日:2005-04-07
申请号:US10496218
申请日:2004-09-13
CPC分类号: C12P21/02 , C07K14/7151 , C12N15/00 , Y10S930/144
摘要: Abstract of the DisclosureThe present invention relates to methods of increasing protein expression levels whereby at least one amino acid in a protein amino acid sequence is substituted for the amino acid, proline. Preferably, the substitution occurs within 15 amino acids, more preferably within 10 amino acids and most preferably within 5 amino acids of a cysteine amino acid residue. The present invention not only includes methods for polypeptides with proline substitutions, but also polynucleotides with codon substitutions for which a codon for any amino acid, except proline, is substituted for a codon encoding for proline.
摘要翻译: 发明内容本发明涉及增加蛋白质表达水平的方法,其中蛋白质氨基酸序列中的至少一个氨基酸被氨基酸脯氨酸取代。 优选地,取代发生在半胱氨酸氨基酸残基的15个氨基酸内,更优选在10个氨基酸内,最优选在5个氨基酸内。 本发明不仅包括具有脯氨酸取代的多肽的方法,而且还包括具有密码子取代的多核苷酸,除了脯氨酸之外,任何氨基酸的密码子都代替编码脯氨酸的密码子。
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公开(公告)号:US20050100989A1
公开(公告)日:2005-05-12
申请号:US10934707
申请日:2004-09-02
申请人: Louise Garone , Stephen Arkinstall , William Brondyk , Robert Campbell , Xuliang Jiang , Sean McKenna , Mark Tepper
发明人: Louise Garone , Stephen Arkinstall , William Brondyk , Robert Campbell , Xuliang Jiang , Sean McKenna , Mark Tepper
IPC分类号: A61K20060101 , C07H21/04 , C07K14/59
CPC分类号: C07K14/59
摘要: A novel FSH mutant with increased glycosylation and longer half-lifes for use in inducing folliculogenesis in human patients is described. The FSH mutant permits the use of lower cumulative doses of FSH to achieve the same or better clinical result.
摘要翻译: 描述了具有增加的糖基化和更长的半衰期用于诱导人类患者卵泡发生的新型FSH突变体。 FSH突变体允许使用较低累积剂量的FSH来实现相同或更好的临床结果。
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公开(公告)号:US20080014635A1
公开(公告)日:2008-01-17
申请号:US11778593
申请日:2007-07-16
CPC分类号: C12P21/02 , C07K14/7151 , C12N15/00 , Y10S930/144
摘要: The present invention relates to methods of increasing protein expression levels whereby at least one amino acid in a protein amino acid sequence is substituted for the amino acid, proline. Preferably, the substitution occurs within 15 amino acids, more preferably within 10 amino acids and most preferably within 5 amino acids of a cysteine amino acid residue. The present invention not only includes methods for polypeptides with proline substitutions, but also polynucleotides with codon substitutions for which a codon for any amino acid, except proline, is substituted for a codon encoding for proline.
摘要翻译: 本发明涉及增加蛋白质表达水平的方法,其中蛋白质氨基酸序列中的至少一个氨基酸被氨基酸脯氨酸取代。 优选地,取代发生在半胱氨酸氨基酸残基的15个氨基酸内,更优选在10个氨基酸内,最优选在5个氨基酸内。 本发明不仅包括具有脯氨酸取代的多肽的方法,而且还包括具有密码子取代的多核苷酸,除了脯氨酸之外,任何氨基酸的密码子都代替编码脯氨酸的密码子。
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公开(公告)号:US20050119465A1
公开(公告)日:2005-06-02
申请号:US10493540
申请日:2002-10-22
申请人: William Brondyk , Xuliang Jiang , Robert Campbell
发明人: William Brondyk , Xuliang Jiang , Robert Campbell
IPC分类号: C12N15/09 , A61K38/00 , A61K38/24 , A61K47/48 , A61P15/08 , C07K20060101 , C07K14/59 , C12N5/00 , C12N5/10 , C12N15/16 , C12N15/63 , C12P21/00 , C12P21/02
摘要: The inventors have designed novel FSH mutants with increased glycosylation and longer half-lives for use in inducing folliculogenesis in human patients. The use of a FSH mutant preparation of the invention permits the use of lower cumulative doses of FSH to achieve the same or better clinical result.
摘要翻译: 本发明人设计了具有增加的糖基化和更长半衰期的新型FSH突变体用于诱导人类患者的卵泡发生。 使用本发明的FSH突变体制剂允许使用较低累积剂量的FSH来实现相同或更好的临床结果。
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公开(公告)号:US07258997B2
公开(公告)日:2007-08-21
申请号:US10496218
申请日:2002-11-21
CPC分类号: C12P21/02 , C07K14/7151 , C12N15/00 , Y10S930/144
摘要: The present invention relates to methods of increasing protein expression levels whereby at least one amino acid in a protein amino acid sequence is substituted for the amino acid, proline. Preferably, the substitution occurs within 15 amino acids, more preferably within 10 amino acids and most preferably within 5 amino acids of a cysteine amino acid residue. The present invention not only includes methods for polypeptides with proline substitutions, but also polynucleotides with codon substitutions for which a codon for any amino acid, except proline, is substituted for a codon encoding for proline.
摘要翻译: 本发明涉及增加蛋白质表达水平的方法,其中蛋白质氨基酸序列中的至少一个氨基酸被氨基酸脯氨酸取代。 优选地,取代发生在半胱氨酸氨基酸残基的15个氨基酸内,更优选在10个氨基酸内,最优选在5个氨基酸内。 本发明不仅包括具有脯氨酸取代的多肽的方法,而且还包括具有密码子取代的多核苷酸,除了脯氨酸之外,任何氨基酸的密码子都代替编码脯氨酸的密码子。
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