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    Methods of expressing LIM mineralization protein in non-osseous cells
    1.
    发明申请
    Methods of expressing LIM mineralization protein in non-osseous cells 有权
    在非骨细胞中表达LIM矿化蛋白的方法

    公开(公告)号：US20100239545A9

    公开(公告)日：2010-09-23

    申请号：US10292951

    申请日：2002-11-13

    申请人： William McKay ,  Scott Boden ,  Sangwook Yoon

    发明人： William McKay ,  Scott Boden ,  Sangwook Yoon

    IPC分类号： A61K48/00 ,  A61F2/44 ,  C07H21/04 ,  C12N5/08

    CPC分类号： A61K48/005 ,  A61K35/12 ,  A61K35/13 ,  A61K48/00 ,  A61K2121/00 ,  A61L27/3804 ,  A61L27/3856 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/78 ,  C07K16/18 ,  C12N2799/022

    摘要： Methods of expressing LIM mineralization protein in non-osseous mammalian cells, such as stem cells or intervertebral disc cells (e.g., cells of the annulus fibrosus, or cells of the nucleus pulposus) are described. The methods involve transfecting the cells with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein operably linked to a promoter. Transfection may be accomplished ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid. Expression of the LIM mineralization protein can stimulate proteoglycan and/or collagen production in cells capable of producing proteoglycyan and/or collagen. Methods for treating disc disease associated with trauma or disc degeneration are also described.

    摘要翻译： 描述了在非骨骼哺乳动物细胞中表达LIM矿化蛋白的方法，例如干细胞或椎间盘细胞（例如，纤维环的细胞或髓核的细胞）。 所述方法涉及用分离的核酸转染细胞，所述分离的核酸包含可操作地连接到启动子的编码LIM矿化蛋白的核苷酸序列。 转染可以通过直接注射病毒或裸露DNA或非病毒载体如质粒在体内或体内完成。 LIM矿化蛋白的表达可以刺激能够产生蛋白多糖和/或胶原的细胞中的蛋白聚糖和/或胶原产生。 还描述了与创伤或椎间盘退变相关的椎间盘疾病的治疗方法。

    Methods of inducing or increasing the expression of proteoglycans such as aggrecan in cells
    2.
    发明申请
    Methods of inducing or increasing the expression of proteoglycans such as aggrecan in cells 审中-公开
    在细胞中诱导或增加蛋白聚糖如聚集蛋白聚糖的表达的方法

    公开(公告)号：US20090304649A9

    公开(公告)日：2009-12-10

    申请号：US11602805

    申请日：2006-11-21

    申请人： William McKay ,  Scott Boden ,  Sangwook Yoon

    发明人： William McKay ,  Scott Boden ,  Sangwook Yoon

    IPC分类号： A61K48/00

    CPC分类号： A61K38/1841 ,  A61K38/1875 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/51

    摘要： Methods of inducing the expression of a proteoglycan such as aggrecan in a cell are described. A method is described which includes transfecting a cell with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein operably linked to a promoter. The LIM mineralization protein can be rLMP, hLMP-1, hLMP-1s, or hLMP-3. Transfection maybe accomplished ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid. The method can be used to induce proteoglycan synthesis in osseous cells or to stimulate proteoglycan and/or collagen production in cells capable of producing proteoglycan and/or collagen (e.g., intervertebral disc cells including cells of the nucleus pulposus and annulus fibrosus).

    摘要翻译： 描述了在细胞中诱导蛋白聚糖如聚集蛋白聚糖表达的方法。 描述了一种方法，其包括用分离的核酸转染细胞，所述分离的核酸包含可操作地连接于启动子的编码LIM矿化蛋白的核苷酸序列。 LIM矿化蛋白可以是rLMP，hLMP-1，hLMP-1或hLMP-3。 转染可以通过直接注射病毒或裸DNA或通过非病毒载体如质粒在体内或体内完成。 该方法可用于在骨细胞中诱导蛋白聚糖合成或刺激能够产生蛋白聚糖和/或胶原的细胞（例如包括髓核细胞和纤维环的椎间盘细胞）中的蛋白聚糖和/或胶原产生。

    Methods of inducing or increasing the expression of proteoglycans such as aggrecan in cells
    3.
    发明申请
    Methods of inducing or increasing the expression of proteoglycans such as aggrecan in cells 审中-公开
    在细胞中诱导或增加蛋白聚糖如聚集蛋白聚糖的表达的方法

    公开(公告)号：US20070134218A1

    公开(公告)日：2007-06-14

    申请号：US11602805

    申请日：2006-11-21

    申请人： William McKay ,  Scott Boden ,  Sangwook Yoon

    发明人： William McKay ,  Scott Boden ,  Sangwook Yoon

    IPC分类号： A61K48/00

    CPC分类号： A61K38/1841 ,  A61K38/1875 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/51

    摘要： Methods of inducing the expression of a proteoglycan such as aggrecan in a cell are described. A method is described which includes transfecting a cell with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein operably linked to a promoter. The LIM mineralization protein can be rLMP, hLMP-1, hLMP-1s, or hLMP-3. Transfection maybe accomplished ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid. The method can be used to induce proteoglycan synthesis in osseous cells or to stimulate proteoglycan and/or collagen production in cells capable of producing proteoglycan and/or collagen (e.g., intervertebral disc cells including cells of the nucleus pulposus and annulus fibrosus).

    摘要翻译： 描述了在细胞中诱导蛋白聚糖如聚集蛋白聚糖表达的方法。 描述了一种方法，其包括用分离的核酸转染细胞，所述分离的核酸包含可操作地连接于启动子的编码LIM矿化蛋白的核苷酸序列。 LIM矿化蛋白可以是rLMP，hLMP-1，hLMP-1或hLMP-3。 转染可以通过直接注射病毒或裸DNA或通过非病毒载体如质粒在体内或体内完成。 该方法可用于在骨细胞中诱导蛋白聚糖合成或刺激能够产生蛋白聚糖和/或胶原的细胞（例如包括髓核细胞和纤维环的椎间盘细胞）中的蛋白聚糖和/或胶原产生。

    Mechanisms of osteoinduction by LIM mineralization protein-1 (LMP-1)
    4.
    发明申请
    Mechanisms of osteoinduction by LIM mineralization protein-1 (LMP-1) 有权
    LIM矿化蛋白-1（LMP-1）诱导骨诱导机制

    公开(公告)号：US20070027081A1

    公开(公告)日：2007-02-01

    申请号：US11385612

    申请日：2006-03-21

    申请人： Jeffrey Marx ,  William McKay ,  Scott Boden

    发明人： Jeffrey Marx ,  William McKay ,  Scott Boden

    IPC分类号： A61K38/17 ,  A61K38/00

    CPC分类号： A61K38/1709 ,  A61K38/1875 ,  A61K2300/00

    摘要： The present invention relates to the methods and compositions for the treatment of subjects having compromised bone conditions. Specifically, the invention relates to combinatorial therapeutic strategies including small molecules and peptide mimics of LIM mineralization proteins, particularly LMP-1, to overcome the dose-related translational barriers for BMP-2 therapeutics.

    摘要翻译： 本发明涉及用于治疗受损骨患者的方法和组合物。 具体地，本发明涉及包括LIM矿化蛋白（特别是LMP-1）的小分子和肽模拟物的组合治疗策略，以克服BMP-2治疗剂的剂量相关翻译屏障。

    Dexamethasone formulations in a biodegradable material
    7.
    发明授权
    Dexamethasone formulations in a biodegradable material 有权
    地塞米松配方可生物降解材料

    公开(公告)号：US08524267B2

    公开(公告)日：2013-09-03

    申请号：US12105864

    申请日：2008-04-18

    申请人： John Zanella ,  Vanja King ,  Christopher M. Hobot ,  Stephen M. Cox ,  William McKay ,  Kathy Remsen

    发明人： John Zanella ,  Vanja King ,  Christopher M. Hobot ,  Stephen M. Cox ,  William McKay ,  Kathy Remsen

    IPC分类号： A61F2/00 ,  A61K9/00

    CPC分类号： A61K31/573 ,  A61K9/0002 ,  A61K9/0024 ,  A61K47/10 ,  A61K47/26 ,  A61K47/34

    摘要： Effective treatments of acute pain for extended periods of time are provided. Through the administration of an effective amount of dexamethasone at or near a target site, one can relieve pain cause by diverse sources, including but not limited to spinal disc herniation (i.e. sciatica), spondilothesis, stenosis, discongenic back pain and joint pain as well as pain that is incidental to surgery. When appropriate formulations are provided within biodegradable polymers, this relief can be continued for at least twenty-five days. In some embodiments, the relief can be for at least fifty days, at least one hundred days, at least one hundred and thirty-five days or at least one hundred and eighty days.

    摘要翻译： 提供了长期急性疼痛的有效治疗方法。 通过在目标部位或附近施用有效量的地塞米松，可以减轻不同来源的疼痛，包括但不限于椎间盘突出症（即坐骨神经痛），脊椎脱位，狭窄，恶性背痛和关节疼痛 作为手术附带的疼痛。 当在可生物降解的聚合物中提供适当的制剂时，这种缓解可以持续至少二十五天。 在一些实施例中，浮雕可以是至少五十天，至少一百天，至少一百三十五天或至少一百八十天。

    Implant depots to deliver growth factors to treat osteoporotic bone
    8.
    发明申请
    Implant depots to deliver growth factors to treat osteoporotic bone 有权
    植入物仓库提供生长因子来治疗骨质疏松的骨骼

    公开(公告)号：US20070259019A1

    公开(公告)日：2007-11-08

    申请号：US11418947

    申请日：2006-05-05

    申请人： William McKay

    发明人： William McKay

    IPC分类号： A61K38/18 ,  A61K38/30 ,  A61F2/02 ,  A61K31/401 ,  A61K31/366 ,  A61K31/22

    CPC分类号： A61L27/227 ,  A61K9/0024 ,  A61L27/24 ,  A61L27/46 ,  A61L27/54 ,  A61L27/56 ,  A61L2300/414 ,  A61L2300/604 ,  C08L89/06

    摘要： The present invention relates to the design and composition of a depot implant for optimal delivery of growth factors to treat osteoporotic bone, in that such depot implant is constructed to be in a cylinder (rod) or sphere shape and have a natural or synthetic polymer scaffold with or without impregnated calcium phosphate particles. The density of the depot is higher than a typical BMP sponge carrier to facilitate it's implantation and slower release of the growth factor. The scaffold is such that it has adequate porosity and pore size to facilitate growth factor seeding and diffusion throughout the whole of the bone structure resulting in increased bone mineral density in the osteoporotic bone. In addition, the shape of the depot implant allows for delivery through a cannula or large bore needle.

    摘要翻译： 本发明涉及用于最佳递送生长因子以治疗骨质疏松性骨的贮库植入物的设计和组成，因为这样的植入物被构造为圆柱（杆）或球形，并且具有天然或合成的聚合物支架 有或没有浸渍的磷酸钙颗粒。 仓库的密度高于典型的BMP海绵载体，以便于其植入和较慢释放生长因子。 支架使得其具有足够的孔隙度和孔径以促进整个骨结构中的生长因子接种和扩散，导致骨质疏松骨中骨矿物质密度增加。 此外，储库植入物的形状允许通过套管或大孔针进行输送。

    Self-cleaning hair brush with replaceable preloaded bristle mat
    10.
    发明申请
    Self-cleaning hair brush with replaceable preloaded bristle mat 审中-公开
    自清洁毛刷与可更换的预载毛刷垫

    公开(公告)号：US20070180638A1

    公开(公告)日：2007-08-09

    申请号：US11702902

    申请日：2007-02-06

    申请人： William McKay

    发明人： William McKay

    IPC分类号： A46B13/00

    CPC分类号： A46B9/06 ,  A01K13/002 ,  A45D24/42 ,  A46B7/026 ,  A46B7/04 ,  A46B9/023 ,  A46B9/065 ,  A46B9/08 ,  A46B13/02 ,  A46B13/023 ,  A46B15/00 ,  A46B15/0002 ,  A46B15/0075 ,  A46B17/04 ,  A46B17/06 ,  A46B2200/102 ,  A46B2200/104 ,  A46B2200/1093

    摘要： A brush for hygienic purposes includes a handle with a lower and an upper portion and a head. The upper portion is sized and shaped to capture the head. Preferably the head is a removable cartridge and includes a flexible base with a plurality bristles and a pad with holes disposed over the bristles. The pad could be a foam or sponge matrix or could be a stack of sheets removable one at a time. Upper portion of the handle is curved to provide a predetermined shape to the head. Optionally, a vibrating device is incorporated in the brush so that the brush can be used as to massage the skin or scalp.

    摘要翻译： 用于卫生目的的刷子包括具有下部和上部部分的手柄以及头部。 上部的尺寸和形状可以捕获头部。 优选地，头部是可移除的盒，并且包括具有多个刷毛的柔性基座和设置在刷毛上方的孔的垫。 垫可以是泡沫或海绵基质，或者可以是一次一片可拆卸的片材。 手柄的上部是弯曲的，以向头部提供预定的形状。 可选地，将振动装置并入刷子中，使得刷子可用于按摩皮肤或头皮。