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公开(公告)号：US20060085866A1
公开(公告)日：2006-04-20
申请号：US11254045
申请日：2005-10-19
IPC分类号： A01K67/027 , C12N5/06
CPC分类号： A01K67/0271 , A01K67/0275 , A01K67/0276 , A01K2207/12 , A01K2227/105 , C07K14/47 , C12N5/0604 , C12N5/16 , C12N15/8509 , C12N2501/235 , C12N2501/415 , C12N2510/00 , C12N2517/02 , C12N2799/026
摘要： Methods of generating modified embryos and mammals by introduction of donor cells into an early stage embryo are provided, such that the resulting embryo and animal generated therefrom has a significant or complete contribution to all tissues from the donor cells and is capable of transmitting the donor cell DNA.
摘要翻译： 提供通过将供体细胞引入早期胚胎来产生修饰的胚胎和哺乳动物的方法，使得由其产生的胚胎和动物对来自供体细胞的所有组织具有显着或完整的贡献，并且能够传递供体细胞 脱氧核糖核酸。
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公开(公告)号：US09149026B2
公开(公告)日：2015-10-06
申请号：US13157728
申请日：2011-06-10
IPC分类号： A01K67/02 , C12N15/873 , A01K67/027 , C12N5/0735 , C12N9/02
CPC分类号： A01K67/0276 , A01K67/0278 , A01K2207/12 , A01K2217/052 , A01K2217/054 , A01K2217/075 , A01K2227/105 , A01K2267/0331 , C12N5/0603 , C12N5/0606 , C12N9/0091 , C12N15/873 , C12N2500/34 , C12N2500/60 , C12N2517/02 , C12N2517/10
摘要： Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will result in fertile XY female animals. Methods and compositions are described for making fertile female animals in an F0 generation from a donor XY cell and a host embryo, as are methods for making F1 progeny that are homozygous for a modification from a heterozygous F0 fertile male and a heterozygous F0 fertile female sibling.
摘要翻译： 描述了从XY供体细胞和合适的宿主胚胎制备表型雌性可育动物的方法和组合物。 提供培养基和方法用于在培养物中维持XY供体细胞，其在引入宿主胚胎之后，并且在合适的宿主中妊娠将导致可育的XY雌性动物。 描述了用于使来自供体XY细胞和宿主胚胎的F0代中的可育雌性动物的方法和组合物，以及用于从杂合的F0可育雄性和杂合的F0可育雌性同胞中进行修饰的F1后代的方法 。
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公开(公告)号：US20110307968A1
公开(公告)日：2011-12-15
申请号：US13157728
申请日：2011-06-10
IPC分类号： A01K67/027 , A01K67/02 , C12N15/873
CPC分类号： A01K67/0276 , A01K67/0278 , A01K2207/12 , A01K2217/052 , A01K2217/054 , A01K2217/075 , A01K2227/105 , A01K2267/0331 , C12N5/0603 , C12N5/0606 , C12N9/0091 , C12N15/873 , C12N2500/34 , C12N2500/60 , C12N2517/02 , C12N2517/10
摘要： Methods and compositions are described for making phenotypically female fertile animals from XY donor cells and suitable host embryos. Culture media and methods are provided for maintaining XY donor cells in culture that after introduction into a host embryo and gestation in a suitable host will result in fertile XY female animals. Methods and compositions are described for making fertile female animals in an F0 generation from a donor XY cell and a host embryo, as are methods for making F1 progeny that are homozygous for a modification from a heterozygous F0 fertile male and a heterozygous F0 fertile female sibling.
摘要翻译： 描述了从XY供体细胞和合适的宿主胚胎制备表型雌性可育动物的方法和组合物。 提供培养基和方法用于在培养物中维持XY供体细胞，其在引入宿主胚胎之后，并且在合适的宿主中妊娠将导致可育的XY雌性动物。 描述了用于使来自供体XY细胞和宿主胚胎的F0代中的可育雌性动物的方法和组合物，以及用于从杂合的F0可育雄性和杂合的F0可育雌性同胞中进行修饰的F1后代的方法 。
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公开(公告)号：US20080078001A1
公开(公告)日：2008-03-27
申请号：US11904558
申请日：2007-09-27
IPC分类号： C12N15/00
CPC分类号： A01K67/0271 , A01K67/0275 , A01K67/0276 , A01K2207/12 , A01K2227/105 , C07K14/47 , C12N5/0604 , C12N5/16 , C12N15/8509 , C12N2501/235 , C12N2501/415 , C12N2510/00 , C12N2517/02 , C12N2799/026
摘要： Methods of generating modified embryos and mammals by introduction of donor cells into an early stage embryo are provided, such that the resulting embryo and animal generated therefrom has a significant contribution to all tissues from the donor cells and is capable of transmitting the donor cell DNA.
摘要翻译： 提供通过将供体细胞引入早期胚胎来产生修饰的胚胎和哺乳动物的方法，使得由其产生的胚胎和动物对来自供体细胞的所有组织具有显着贡献，并且能够传递供体细胞DNA。
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公开(公告)号：US20080078000A1
公开(公告)日：2008-03-27
申请号：US11904373
申请日：2007-09-27
CPC分类号： A01K67/0271 , A01K67/0275 , A01K67/0276 , A01K2207/12 , A01K2227/105 , C07K14/47 , C12N5/0604 , C12N5/16 , C12N15/8509 , C12N2501/235 , C12N2501/415 , C12N2510/00 , C12N2517/02 , C12N2799/026
摘要： Methods of generating modified embryos and mammals by introduction of donor cells into an early stage embryo are provided, such that the resulting embryo and animal generated therefrom has a significant contribution to all tissues from the donor cells and is capable of transmitting the donor cell DNA.
摘要翻译： 提供通过将供体细胞引入早期胚胎来产生修饰的胚胎和哺乳动物的方法，使得由其产生的胚胎和动物对来自供体细胞的所有组织具有显着贡献，并且能够传递供体细胞DNA。
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公开(公告)号：US20080028479A1
公开(公告)日：2008-01-31
申请号：US11904559
申请日：2007-09-27
IPC分类号： C12N15/00
CPC分类号： A01K67/0271 , A01K67/0275 , A01K67/0276 , A01K2207/12 , A01K2227/105 , C07K14/47 , C12N5/0604 , C12N5/16 , C12N15/8509 , C12N2501/235 , C12N2501/415 , C12N2510/00 , C12N2517/02 , C12N2799/026
摘要： Methods of generating modified embryos and mammals by introduction of donor cells into an early stage embryo are provided, such that the resulting embryo and animal generated therefrom has a significant contribution to all tissues from the donor cells and is capable of transmitting the donor cell DNA.
摘要翻译： 提供了通过将供体细胞引入早期胚胎来产生修饰的胚胎和哺乳动物的方法，使得所产生的胚胎和由其产生的动物对来自供体细胞的所有组织具有显着贡献，并且能够传递供体细胞DNA。
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7.发明申请公开(公告)号：US20060064770A1
公开(公告)日：2006-03-23
申请号：US11227848
申请日：2005-09-15
IPC分类号： A01K67/027 , C12N5/06 , C12N15/87
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2267/0393 , C12N15/907 , C12N2830/00
摘要： Methods for modifying a mammalian cell target such that the presence of a Y chromosome is detectable. The method includes construction of a targeting vector with a detectable marker that can recombine or insert into a pre-selected site on the Y chromosome, allowing the presence of the Y chromosome to be detectable through the presence of the integrated detectable marker. The method can be used to distinguish male from female embryos.
摘要翻译： 用于修饰哺乳动物细胞靶的方法，使得Y染色体的存在是可检测的。 该方法包括构建具有可重组或插入到Y染色体上的预先选择的位点的可检测标记的靶向载体，允许Y染色体的存在通过存在整合的可检测标记来检测。 该方法可用于区分雄性和雌性胚胎。
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公开(公告)号：US20050101017A1
公开(公告)日：2005-05-12
申请号：US10705432
申请日：2003-11-10
CPC分类号： C12N15/907 , A01K2217/05 , A01K2227/105 , C12N15/8509
摘要： Methods of improving gene targeting comprising using a ubiquitin promoter to drive drug resistance gene expression are provided. Such improvements include increasing ES cell colony survival and targeting frequency.
摘要翻译： 提供了改进基因靶向的方法，其包括使用泛素启动子来驱动药物抗性基因表达。 这些改善包括增加ES细胞群落存活和靶向频率。
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公开(公告)号：US20050144655A1
公开(公告)日：2005-06-30
申请号：US10415440
申请日：2001-10-31
IPC分类号： C12N15/00 , A01K67/027 , C12N5/10 , C12N15/09 , C12N15/79 , C12N15/90 , C12Q1/68 , C12N5/06 , C12N15/85
CPC分类号： C12N15/85 , A01K67/0275 , A01K2217/05 , A01K2227/105 , C12N5/0606 , C12N15/79 , C12N15/907 , C12N2510/00 , C12N2810/10 , C12Q1/6827
摘要： A method for engineering and utilizing large DNA vectors to target, via homologous recombination, and modify, in any desirable fashion, endogenous genes and chromosomal loci in eukaryotic cells. These large DNA targeting vectors for eukaryotic cells, termed LTVECs, are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Also provided is a rapid and convenient method of detecting eukaryotic cells in which the LTVEC has correctly targeted and modified the desired endogenous genes(s) or chromosomal locus (loci) as well as the use of these cells to generate organisms bearing the genetic modification.
摘要翻译： 一种用于工程化和利用大型DNA载体通过同源重组靶向并以任何所需方式修饰真核细胞中的内源基因和染色体基因座的方法。 用于真核细胞的这些大的DNA靶向载体，称为LTVEC，衍生自克隆基因组DNA的片段，大于通常用于在真核细胞中进行同源靶向的其它方法通常使用的那些。 还提供了检测真核细胞的快速和方便的方法，其中LTVEC已经正确靶向和修饰所需的内源基因或染色体基因座（基因座）以及这些细胞用于产生具有遗传修饰的生物体。
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公开(公告)号：US20070275466A1
公开(公告)日：2007-11-29
申请号：US11787969
申请日：2007-04-17
IPC分类号： C12N15/89
CPC分类号： C12N15/85 , A01K67/0275 , A01K2217/05 , A01K2217/075 , A61D19/04 , C12N15/907 , C12N2800/30 , C12N2830/42 , C12N2840/44
摘要： Methods of creating mutations in genomic exons by inserting introns into the genomic exons via homologous recombination. Also, methods are provided for introducing modifications into genomic exons by inserting introns into the genomic exons via homologous recombination such that a mature mRNA transcript produced from a genomic region of the genome comprising the genomic exon does not contain the modification are provided. The methods provide for a rapid method for introducing mutations and/or modifications of any type into a mammalian cell genome.
摘要翻译： 通过将内含子通过同源重组插入到基因组外显子中而在基因组外显子中产生突变的方法。 此外，提供了通过将内含子通过同源重组插入到基因组外显子中而将修饰引入基因组外显子的方法，从而提供了从包含基因组外显子的基因组的基因组区域产生的成熟mRNA转录物不含修饰。 所述方法提供了用于将任何类型的突变和/或修饰引入哺乳动物细胞基因组的快速方法。
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