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6 条记录 (耗时 0.017 秒)

    Method for increasing the gene expression of saccharose synthase
    1.
    发明授权
    Method for increasing the gene expression of saccharose synthase 失效
    增加蔗糖合酶基因表达的方法

    公开(公告)号：US06444454B1

    公开(公告)日：2002-09-03

    申请号：US09486291

    申请日：2000-05-19

    申请人： Wolf-Bernd Frommer ,  Henning Schrader ,  Lothar Elling

    发明人： Wolf-Bernd Frommer ,  Henning Schrader ,  Lothar Elling

    IPC分类号： C12N900

    CPC分类号： C12N9/1062 ,  C12N15/81

    摘要： The invention relates to a method for increasing the gene expression of saccharose synthase. To this end the invention provides for the expression of the saccharose synthase gene to be placed under the control of a proton-ATPase promoter. According to a preferred version of the invention gene expression of the saccharose synthase is also increased by increasing the number of copies of the saccharose synthase gene and of the proton-ATPase promoter.

    摘要翻译： 本发明涉及增加蔗糖合酶基因表达的方法。 为此，本发明提供将蔗糖合成酶基因置于质子-ATP酶启动子控制下的表达。 根据本发明的优选方案，蔗糖合成酶的基因表达也通过增加蔗糖合酶基因和质子-ATPase启动子的拷贝数而增加。

    Purified saccharose-synthase, process for its production and its use
    2.
    发明授权
    Purified saccharose-synthase, process for its production and its use 失效
    纯化的蔗糖合成酶，其生产和使用的过程

    公开(公告)号：US5750389A

    公开(公告)日：1998-05-12

    申请号：US367178

    申请日：1995-01-06

    申请人： Lothar Elling ,  Maria-Regina Kula

    发明人： Lothar Elling ,  Maria-Regina Kula

    IPC分类号： C07H19/10 ,  C07H19/20 ,  C12N9/10 ,  C12P19/30 ,  C12P19/38

    CPC分类号： C07H19/10 ,  C07H19/20 ,  C12N9/1062 ,  C12P19/30 ,  C12P19/38

    摘要： Nucleotide sugars, especially UDP, ADP, CDP or TDP saccharoses can be enzymatically obtained by the reaction of nucleoside diphosphates with di or trisaccharides with a saccharose synthase in which the virtual absence of nucleoside phosphatases (0.1% or less) can be ensured by special purification methods and sensitive detection. The purification of the raw extract, obtained preferably from rice grains, comprises especially the application of the ultra-filtered extract containing 50 mM KCl with a pH 8 on a sepharose Q column and a gradient elution out of the column at a pH 8 with 50 to 500 mM KCl.

    摘要翻译： PCT No.PCT / DE93 / 00562 Sec。 371 1995年1月6日第 102（e）日期1995年1月6日PCT Filed June 26，1993 PCT Pub。 第WO94 / 01540BC号公报 日期1994年1月20日核苷酸糖，特别是UDP，ADP，CDP或TDP蔗糖可以通过核苷二磷酸与二糖或三糖与蔗糖合酶的反应获得酶，其中虚拟不存在核苷磷酸酶（0.1％或更少）可以 通过特殊的净化方法和敏感检测来保证。 优选从米粒获得的原始提取物的纯化特别包括在Sepharose Q柱上应用含有pH8的50mM KCl的超滤提取物，并在pH8下用柱梯度洗脱，用50 至500mM KCl。

    Nucleotide-sugar-synthesizing enzymes from nonparasitic protists
    3.
    发明授权
    Nucleotide-sugar-synthesizing enzymes from nonparasitic protists 失效
    来自非寄生性原生生物的核苷酸 - 糖合成酶

    公开(公告)号：US07217550B2

    公开(公告)日：2007-05-15

    申请号：US10436382

    申请日：2003-05-12

    申请人： Thomas Kiy ,  Lothar Elling ,  Maria Regina Kula

    发明人： Thomas Kiy ,  Lothar Elling ,  Maria Regina Kula

    IPC分类号： C12N9/12

    CPC分类号： C12N9/1241 ,  Y10S435/947

    摘要： The present invention relates to nucleotide-sugar-synthesizing enzymes (enzymes with nucleotidyltransferase or pyrophosphorylase activity) from nonparasitic protists, to a process for the preparation thereof and to the use thereof for preparing nucleotide-sugars. The enzymes according to the invention make possible or greatly simplify the enzymatic preparation of various nucleotide-sugars on the industrial scale from low-cost precursors. It is possible with the aid of the discovered enzymes to prepare, for example, GDP-fucose, GDP-mannose, UDP-glucose, UDP-glucosamine, UDP-galactose, UDP-galactosamine, UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in economic quantities.

    摘要翻译： 本发明涉及从非寄生型原生生物的核苷酸 - 糖合成酶（具有核苷酸转移酶或焦磷酸化酶活性的酶），其制备方法及其制备核苷酸 - 糖的用途。 根据本发明的酶使得可能或大大简化了低成本前体在工业规模上的各种核糖 - 糖的酶制剂。 借助所发现的酶可以制备例如GDP-岩藻糖，GDP-甘露糖，UDP-葡萄糖，UDP-葡糖胺，UDP-半乳糖，UDP-半乳糖胺，UDP-N-乙酰氨基葡萄糖和UDP-N- 乙酰半乳糖经济量。

    UDP-glucose pyro phosphorylase enzymes from nonparasitic protozoa
    4.
    发明授权
    UDP-glucose pyro phosphorylase enzymes from nonparasitic protozoa 失效
    来自非寄生虫原生动物的UDP-葡萄糖焦磷酸化酶

    公开(公告)号：US6143539A

    公开(公告)日：2000-11-07

    申请号：US796697

    申请日：1997-03-03

    申请人： Thomas Kiy ,  Lothar Elling ,  Maria Regina Kula

    发明人： Thomas Kiy ,  Lothar Elling ,  Maria Regina Kula

    IPC分类号： C12N9/10 ,  C12N9/12 ,  C12P19/30 ,  C12R1/90

    CPC分类号： C12N9/1241 ,  Y10S435/947

    摘要： The present invention relates to nucleotide-sugar-synthesizing enzymes (enzymes with nucleotidyltransferase or pyrophosphorylase activity) from nonparasitic protists, to a process for the preparation thereof and to the use thereof for preparing nucleotide-sugars. The enzymes according to the invention make possible or greatly simplify the enzymatic preparation of various nucleotide-sugars on the industrial scale from low-cost precursors. It is possible with the aid of the discovered enzymes to prepare, for example, GDP-fucose, GDP-mannose, UDP-glucose, UDP-glucosamine, UDP-galactose, UDP-galactosamine, UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in economic quantities.

    摘要翻译： 本发明涉及从非寄生型原生生物的核苷酸 - 糖合成酶（具有核苷酸转移酶或焦磷酸化酶活性的酶），其制备方法及其制备核苷酸 - 糖的用途。 根据本发明的酶使得可能或大大简化了低成本前体在工业规模上的各种核糖 - 糖的酶制剂。 借助所发现的酶可以制备例如GDP-岩藻糖，GDP-甘露糖，UDP-葡萄糖，UDP-葡糖胺，UDP-半乳糖，UDP-半乳糖胺，UDP-N-乙酰氨基葡萄糖和UDP-N- 乙酰半乳糖经济量。