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公开(公告)号：US06444454B1
公开(公告)日：2002-09-03
申请号：US09486291
申请日：2000-05-19
IPC分类号： C12N900
CPC分类号： C12N9/1062 , C12N15/81
摘要： The invention relates to a method for increasing the gene expression of saccharose synthase. To this end the invention provides for the expression of the saccharose synthase gene to be placed under the control of a proton-ATPase promoter. According to a preferred version of the invention gene expression of the saccharose synthase is also increased by increasing the number of copies of the saccharose synthase gene and of the proton-ATPase promoter.
摘要翻译： 本发明涉及增加蔗糖合酶基因表达的方法。 为此，本发明提供将蔗糖合成酶基因置于质子-ATP酶启动子控制下的表达。 根据本发明的优选方案，蔗糖合成酶的基因表达也通过增加蔗糖合酶基因和质子-ATPase启动子的拷贝数而增加。
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2.发明授权DNA sequences for an amino acid transporter, plasmids, bacteria, yeasts and plants containing a transporter and their use 失效含有转运蛋白的氨基酸转运蛋白，质粒，细菌，酵母和植物及其用途的DNA序列
公开(公告)号：US5719043A
公开(公告)日：1998-02-17
申请号：US362512
申请日：1995-01-05
申请人： Wolf-Bernd Frommer
发明人： Wolf-Bernd Frommer
IPC分类号： C12N15/09 , A01H5/00 , C07K14/415 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/29 , C12N15/81 , C12N15/82 , C12N15/11 , C12N15/63
CPC分类号： C07K14/415 , C12N15/81 , C12N15/8241
摘要： There are described DNA sequences that contain the coding region of amino acid transporters whose introduction in a plant genome modifies the transfer of metabolites in transgenic plants, plasmids, bacteria, yeasts and plants containing these DNA sequences, as well as their use.
摘要翻译： PCT No.PCT / EP93 / 01736 Sec。 371 1995年1月5日第 102（e）日期1995年1月5日PCT提交1993年7月1日PCT公布。 出版物WO94 / 01559 日期1994年1月20日描述了含有氨基酸转运蛋白的编码区的DNA序列，其在植物基因组中的引入修饰了含有这些DNA序列的转基因植物，质粒，细菌，酵母和植物中代谢物的转移，以及它们 使用。
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公开(公告)号：US20070136825A1
公开(公告)日：2007-06-14
申请号：US10490153
申请日：2002-09-13
申请人： Wolf-Bernd Frommer , Marcus Fehr , Sylvie Lalonde
发明人： Wolf-Bernd Frommer , Marcus Fehr , Sylvie Lalonde
IPC分类号： A01K67/027 , C07H21/04 , C12P21/04 , C12N15/82 , C12N5/04 , C07K14/435
CPC分类号： C07K14/43595 , A01K2217/05 , C07K2319/00 , C07K2319/20 , C07K2319/60 , G01N33/542
摘要： Disclosed are fusion proteins comprising two detection portions and a periplasmic binding protein (PBP) portion between this two detection portions which undergoes a conformational change upon binding of a compound. Also described are nucleic acid molecules comprising a nucleotide sequence encoding such a fusion protein as well as expression cassettes, vectors, transgenic plants and transgenic non-human animals comprising said nucleic acid molecules. In addition, described are methods for detecting an analyte in a sample or in a cell using said fusion protein, and optionally, a control sensor allowing a calibration of analyte detection made by said fusion protein. Also described are such control sensors and nucleic acid molecules encoding them, as well as diagnostic compositions, kits and uses of the fusion protein and the control sensor for in vitro or in vivo analyte detection and preparing a diagnostic composition.
摘要翻译： 公开了在两个检测部分之间包含两个检测部分和周质结合蛋白（PBP）部分的融合蛋白，其在化合物结合时经历构象变化。 还描述了包含编码这种融合蛋白的核苷酸序列以及包含所述核酸分子的表达盒，载体，转基因植物和转基因非人动物的核酸分子。 此外，描述了使用所述融合蛋白检测样品或细胞中的分析物的方法，以及任选的允许校准由所述融合蛋白产生的分析物检测的控制传感器的方法。 还描述了这样的控制传感器和编码它们的核酸分子，以及用于体外或体内分析物检测和制备诊断组合物的融合蛋白和控制传感器的诊断组合物，试剂盒和用途。
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4.发明申请Nucleic acids that can be used to create plants with a modified metabolite content 审中-公开可用于产生具有修饰的代谢物含量的植物的核酸公开(公告)号：US20050188433A1
公开(公告)日：2005-08-25
申请号：US10994377
申请日：2004-11-23
IPC分类号： A01H5/00 , C07K14/415 , C07K16/16 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/29 , C12N15/82 , A01H1/00 , C07H21/04 , C07K14/705
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8251 , C12N15/8274 , Y02A40/146
摘要： The invention relates to a nucleic acid that encodes a plant transporter of the inner mitochondrial membrane, in particular a member of the mitochondrial carrier family (MCF), and uses of this nucleic acid. The invention furthermore relates to a fragment of the nucleic acid, a construct containing the nucleic acid or a fragment thereof, and a host cell that contains the nucleic acid, the fragment, or the construct. The present invention furthermore relates to a method for producing a transgenic plant by using the nucleic acid, a fragment thereof, or a construct containing the nucleic acid or a fragment thereof, as well as a method for modulating the transport properties of the inner mitochondrial membrane of a plant, a plant part, a plant cell and/or seeds.
摘要翻译： 本发明涉及编码内线粒体膜，特别是线粒体载体家族（MCF）成员的植物转运蛋白的核酸，以及该核酸的用途。 本发明还涉及核酸的片段，含有核酸或其片段的构建体和含有核酸，片段或构建体的宿主细胞。 本发明还涉及通过使用核酸，其片段或含有该核酸或其片段的构建体来生产转基因植物的方法，以及调节内线粒体膜的转运性质的方法 的植物，植物部分，植物细胞和/或种子。
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5.发明授权DNA sequences with oligosaccharide transporter, plasmids bacteria and plants containing a transporter as well as a process for the preparation and transformation of yeast strains for identification of the transporter 失效具有寡糖转运蛋白，质粒细菌和含有转运蛋白的植物的DNA序列以及用于鉴定转运蛋白的酵母菌株的制备和转化的方法
公开(公告)号：US5981181A
公开(公告)日：1999-11-09
申请号：US786555
申请日：1997-01-21
申请人： Wolf-Bernd Frommer , Jorg Riesmeier
发明人： Wolf-Bernd Frommer , Jorg Riesmeier
IPC分类号： A01H5/00 , C07K14/415 , C12N1/19 , C12N1/21 , C12N9/10 , C12N9/26 , C12N15/09 , C12N15/29 , C12N15/70 , C12N15/81 , C12N15/82 , C12R1/01 , C12R1/19 , C12R1/865 , C12Q1/68 , C07H21/04 , C07K5/00
CPC分类号： C12N9/2408 , C07K14/415 , C12N15/70 , C12N15/81 , C12N15/8245 , C12N9/1062
摘要： There are described DNA sequences, that contain the coding region of an oligosaccharide transporter, whose introduction in a plant genome modifies the formation and transfer of storage materials in transgenic plants, plasmids, bacteria and plants containing these DNA sequences, a process for the preparation and transformation of yeast strains, that makes possible the identification of the DNA sequences of the plant oligosaccharide transporter of the invention, as well as the use of DNA sequences of the invention.
摘要翻译： 描述了含有寡糖转运蛋白的编码区的DNA序列，其在植物基因组中的引入修饰了含有这些DNA序列的转基因植物，质粒，细菌和植物中的储存材料的形成和转移，制备方法和 可以鉴定本发明的植物寡糖转运蛋白的DNA序列，以及使用本发明的DNA序列的酵母菌株的转化。
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6.发明授权DNA sequences with oligosaccharide transporter, plasmids, bacteria and plants containing a transporter as well as a process for the preparation and transformation of yeast strains for identification of the transporter 失效含有转运蛋白的寡糖转运蛋白，质粒，细菌和植物的DNA序列以及用于鉴定转运蛋白的酵母菌株的制备和转化的方法
公开(公告)号：US5608146A
公开(公告)日：1997-03-04
申请号：US356340
申请日：1994-12-21
申请人： Wolf-Bernd Frommer , Jorg Riesmeier
发明人： Wolf-Bernd Frommer , Jorg Riesmeier
IPC分类号： A01H5/00 , C07K14/415 , C12N1/19 , C12N1/21 , C12N9/10 , C12N9/26 , C12N15/09 , C12N15/29 , C12N15/70 , C12N15/81 , C12N15/82 , C12R1/01 , C12R1/19 , C12R1/865 , C12N5/04 , C12N5/10
CPC分类号： C12N9/2408 , C07K14/415 , C12N15/70 , C12N15/81 , C12N15/8245 , C12N9/1062
摘要： There are described DNA sequences, that contain the coding region of an oligosaccharide transporter, whose introduction in a plant genome modifies the formation and transfer of storage materials in transgenic plants, plasmids, bacteria and plants containing these DNA sequences, a process for the preparation and transformation of yeast strains, that makes possible the identification of the DNA sequences of the plant oligosaccharide transporter of the invention, as well as the use of DNA sequences of the invention.
摘要翻译： PCT No.PCT / EP93 / 01604 Sec。 371日期1994年12月21日 102（e）日期1994年12月21日PCT提交1993年6月22日PCT公布。 第WO94 / 00574号公报 日期1994年1月6日描述了含有寡糖转运蛋白的编码区的DNA序列，其在植物基因组中的引入修饰了含有这些DNA序列的转基因植物，质粒，细菌和植物中的储存材料的形成和转移， 用于制备和转化酵母菌株的方法，其可以鉴定本发明的植物低聚糖转运蛋白的DNA序列，以及本发明的DNA序列的使用。
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7.发明申请Method for producing a transgenic plant having modified transport of substances 审中-公开用于生产具有改变的物质运输的转基因植物的方法公开(公告)号：US20050235376A1
公开(公告)日：2005-10-20
申请号：US10514513
申请日：2003-05-12
IPC分类号： C07K14/415 , C12N15/82 , C07H21/04 , A01H1/00
CPC分类号： C12N15/8251 , C07K14/415 , C12N15/8243 , C12N15/8245
摘要： The invention relates to a method for producing a transgenic plant in which the capability for incorporating undesired nitrogen compounds, especially amino acids, in the storage organs is reduced compared with the wild type, comprising the steps: introducing at least one DNA sequence and/or at least one RNA sequence corresponding to the DNA sequence and/or a mixed sequence composed of DNA and RNA nucleotides corresponding to the DNA sequence with the coding region for an amino acid transporter or parts thereof into a plant cell, wherein the DNA and/or RNA sequence and/or mixed sequence is used in sense or antisense orientation and the expression of an endogenous amino acid transporter gene is prevented or reduced, and regenerating a plant from this plant cell, wherein the DNA and/or RNA sequence is a sequence from Beta vulgaris.
摘要翻译： 本发明涉及一种用于生产转基因植物的方法，其中与野生型相比，在储存器官中掺入不需要的氮化合物，特别是氨基酸的能力降低，包括以下步骤：引入至少一个DNA序列和/或 对应于DNA序列的至少一个RNA序列和/或由对应于具有氨基酸转运蛋白或其部分的编码区的DNA序列的DNA和RNA核苷酸组成的混合序列进入植物细胞，其中所述DNA和/或 RNA序列和/或混合序列以正义或反义方向使用，并且内源氨基酸转运蛋白基因的表达被预防或减少，并且从该植物细胞再生植物，其中所述DNA和/或RNA序列是来自 Beta寻常型
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8.发明授权
公开(公告)号：US5750362A
公开(公告)日：1998-05-12
申请号：US663222
申请日：1996-08-05
CPC分类号： A01N61/00 , C12N15/8241 , C12Q1/025 , C12Q1/18 , G01N2430/20
摘要： A method for identifying substances with a potential herbicidal and growth-regulating action by means of plant transporter proteins (transporters), the use thereof and substances identifiable by the method and having a herbicidal and growth-regulating action are described.
摘要翻译： PCT No.PCT / EP94 / 04174 Sec。 371日期：1996年8月5日 102（e）日期1996年8月5日PCT 1994年12月15日PCT公布。 WO95 / 16913PC PCT出版物 日期1995年6月22日描述了通过植物转运蛋白（转运蛋白）鉴定具有潜在除草和生长调节作用的物质的方法，其使用方法和通过该方法可鉴定并具有除草和生长调节作用的物质。
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公开(公告)号：US5436393A
公开(公告)日：1995-07-25
申请号：US995911
申请日：1992-12-22
IPC分类号： A01H1/00 , C07K14/415 , C12N1/21 , C12N5/10 , C12N9/14 , C12N15/09 , C12N15/29 , C12N15/82 , A01H1/04 , C07H21/04 , C12N5/00 , C12N15/00
CPC分类号： C12N15/8222 , C07K14/415 , C12N15/8227 , C12N15/8235 , C12N9/14
摘要： There is described a new DNA sequence of an expression cassette on which the potato tuber specific regulatory regions are localised as well as the transfer of this DNA sequence into the plant genome using agrobacteria as transfer micro-organisms. The DNA sequence contains a patatin gene with a patatin gene promoter. The transfer DNA sequence acts both for regulating endogenous as well as for preparation of heterologous products in crops.
摘要翻译： 描述了一种表达盒的新DNA序列，其上马铃薯块茎特异性调节区域被定位，以及使用农杆菌作为转移微生物将该DNA序列转移到植物基因组中。 DNA序列含有具有patatin基因启动子的patatin基因。 转移DNA序列既用于调节内源物质以及在作物中制备异源产物。
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10.发明授权DNA sequences for an amino acid transporter plasmids, bacteria, yeasts and plants containing a transporter and their use 失效含有转运蛋白的氨基酸转运体质粒，细菌，酵母和植物的DNA序列及其用途公开(公告)号：US06809233B2
公开(公告)日：2004-10-26
申请号：US09854774
申请日：2001-05-14
申请人： Wolf-Bernd Frommer
发明人： Wolf-Bernd Frommer
IPC分类号： C12N510
CPC分类号： C07K14/415 , C12N15/81 , C12N15/8241
摘要： There are described DNA sequences, that contain the coding region of amino acid transporters whose, introduction in a plant genome modifies the transfer of metabolites in transgenic plants, plasmids, bacteria, yeasts and plants containing these DNA sequences, as well as their use.
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