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1.发明授权Microbiologically produced D-2-hydroxy-4-methylpentanoic acid dehydrogenase, process for its production and its use 失效微生物生产的D-2-羟基-4-甲基戊酸脱氢酶，其生产及其应用的过程
公开(公告)号：US4609623A
公开(公告)日：1986-09-02
申请号：US617738
申请日：1984-06-06
CPC分类号： C12N9/0006 , C12P11/00 , C12P7/40 , C12P7/42 , Y10S435/822 , Y10S435/853 , Y10S435/856
摘要： The enzyme D-2-hydroxy-4-methylpentanoic acid dehydrogenase has been prepared by culturing readily available Lactobacillus or Leuconostoc, microorganisms, such as Lactobacillus casei ssp. pseudoplantarum and Leuconostoc mesenteroides. The microbiologically produced enzyme has special characteristics and is capable of converting D-2-hydroxycarboxylic acids, such as D-2-hydroxy-4-methylpentanoic acid to the corresponding 2-ketocarboxylic acid and is capable of enzymatically converting 2-ketocarboxylic acids, such as 2-keto-4-methylpentanoic acid and 2-ketobutyric acid to the corresponding D-2-hydroxycarboxylic acid.
摘要翻译： 酶D-2-羟基-4-甲基戊酸脱氢酶已经通过培养容易获得的乳酸杆菌或明串珠菌，微生物如干酪乳杆菌（Sact。 假根植物和肠系膜梭菌。 微生物产生的酶具有特殊的特性，能够将D-2-羟基羧酸如D-2-羟基-4-甲基戊酸转化为相应的2-酮羧酸，并且能够酶促转化2-酮羧酸，如 作为2-酮-4-甲基戊酸和2-酮丁酸转化成相应的D-2-羟基羧酸。
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2.发明授权Microbiologically produced .alpha.-acetylamino cinnamic acid acylase, method of its production and its use 失效微生物生产的α-乙酰氨基肉桂酸酰基转移酶，其生产方法及其应用
公开(公告)号：US4877734A
公开(公告)日：1989-10-31
申请号：US66492
申请日：1987-06-26
CPC分类号： C12P13/04 , C12N9/80 , Y10S435/84
摘要： A microbiologically produced .alpha.-acetylamino cinnamic acid acylase and a method for its production from Brevibacterium species NCIB 12246 or NCIB 12247. The new enzyme can be used as constituent of a coupled enzyme system for enzymatic conversions which run via the intermediary stage .alpha.-imino-.beta.-phenylpropionic acid or phenylpyruvic acid.
摘要翻译： 微生物产生的α-乙酰氨基肉桂酸酰基转移酶及其从短杆菌属物种NCIB 12246或NCIB 12247生产的方法。该新酶可以用作通过中间阶段α-亚氨基转移酶进行的酶转化的偶联酶系统的组分， β-苯基丙酸或苯基丙酮酸。
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3.发明授权Microbiologically produced d(-)-mandelate-dehydrogenase process for obtaining it and its use 失效微生物生产的d（ - ） - 扁桃酸脱氢酶方法获得它及其用途
公开(公告)号：US4824781A
公开(公告)日：1989-04-25
申请号：US917440
申请日：1986-10-10
CPC分类号： C12N9/0006 , C12P7/42 , Y10S435/853
摘要： The subject matter of the invention is a new D(-)-mandelate-dehydrogenase and obtaining it from Lactobacillus curvatus DSM 20019 (obtainable from the German Collection of Microorganisms). The new enzyme can be used for the enzymatic conversion of D(-)-mandelic acid and various other D-2-hydroxycarboxylic acids into the corresponding 2-ketocarboxylic acids or from benzoyl formate and various other 2-ketocarboxylic acids conversion to the corresponding D-2-hydroxycarboxylic acids.
摘要翻译： 本发明的主题是新的D（ - ） - 扁桃酸脱氢酶，并从曲霉DSM 20019获得（可从德国微生物收集库获得）。 该新酶可用于将D（ - ） - 扁桃酸和各种其他D-2-羟基羧酸酶促转化成相应的2-酮羧酸或甲酸甲苯酯和各种其他2-酮羧酸转化为相应的D -2-羟基羧酸。
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4.发明授权L-2-hydroxy-4-methylpentanoic acid-dehydrogenase, process for obtaining it and its use 失效L-2-羟基-4-甲基戊酸脱氢酶，其获得方法及其用途
公开(公告)号：US4530903A
公开(公告)日：1985-07-23
申请号：US531725
申请日：1983-09-13
CPC分类号： C12N9/0006 , C12P11/00 , C12P7/40 , C12P7/42 , Y10S435/853
摘要： The purpose of the invention is the new enzyme L-2-hydroxy-4-methylpentanoic acid-dehydrogenase and its recovery from Lactobacillus confuses. The new enzyme can be used to enzymatically change L-2-hydroxy-4-methylpentanoic acid and various other L-2-hydroxy-carboxylic acids into the corresponding 2-ketocarboxylic acids or 2-keto-4-methylpentanoic acid and various other 2-ketocarboxylic acids into the corresponding L-2-hydroxycarboxylic acids.
摘要翻译： 本发明的目的是新的酶L-2-羟基-4-甲基戊酸脱氢酶及其从乳杆菌混合物的回收。 新的酶可用于将L-2-羟基-4-甲基戊酸和各种其它的L-2-羟基羧酸酶化成相应的2-酮基羧酸或2-酮-4-甲基戊酸和各种其它2 - 酮羧酸转化成相应的L-2-羟基羧酸。
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公开(公告)号：US5416019A
公开(公告)日：1995-05-16
申请号：US186501
申请日：1994-01-26
CPC分类号： C12N9/0018 , C12N9/0028 , C12P13/04 , C12P13/222 , C12R1/01
摘要： The invention is directed to obtaining phenylalanine-dehydrogenase containing microorganisms by a special selection process comprising obtaining microorganisms, especially strains of Rhodococcus and the phenylalanine-dehydrogenase obtained in them. With the help of this enzyme there are obtained L-.alpha.-aminoacids from the corresponding ketocarboxylic acids by reductive amination.
摘要翻译： 本发明涉及通过特殊的选择方法获得含苯丙氨酸脱氢酶的微生物，该方法包括获得微生物，尤其是获得的红球菌菌株和苯丙氨酸脱氢酶。 在这种酶的帮助下，通过还原胺化得到相应的酮羧酸的L-α-氨基酸。
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6.发明授权Microbiologically produced L-phenylalanine-dehydrogenase, process for its recovery and use 失效微生物生产的L-苯丙氨酸脱氢酶，其回收利用过程
公开(公告)号：US4590161A
公开(公告)日：1986-05-20
申请号：US583325
申请日：1984-02-24
CPC分类号： C12N9/0018 , C12N9/0028 , C12P13/12 , C12P13/222 , Y10S435/84
摘要： The invention is directed to a microbiologically produced L-phenylalanine-dehydrogenase and a process for its recovery from Brevibacterium species DSM 2448. The new enzyme can be used for the enzymatic conversion of phenyl pyruvic acid, p-hydroxyphenyl pyruvic aid, indolyl pyruvic acid or 2-keto-4-(methylmercapto)-butyric acid into the corresponding L-.alpha.-aminocarboxylic acids.
摘要翻译： 本发明涉及微生物学产生的L-苯丙氨酸脱氢酶及其从短杆菌属物种DSM 2448中回收的方法。该新酶可用于苯丙酮酸，对羟基苯基丙酮酸，吲哚基丙酮酸或 2-甲基-4-（甲基巯基） - 丁酸转化成相应的L-α-氨基羧酸。
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7.发明授权Process for producing ECoRI restriction endonuclease with E.coli mutants 失效用{i大肠杆菌{b突变体生产ECoRI限制性内切核酸酶的方法
公开(公告)号：US4064011A
公开(公告)日：1977-12-20
申请号：US778261
申请日：1977-03-16
申请人： Hubert Mayer , Horst Schutte
发明人： Hubert Mayer , Horst Schutte
CPC分类号： C12N9/22 , Y10S435/815 , Y10S435/849
摘要： ECoRI restriction endonuclease is produced with certain mutant strains of E.coli which are not antibiotic resistant and do produce the said enzyme which is obtained from the E.coli mutant cells without destroying the cells by extraction procedures.
摘要翻译： 使用某些不具有抗生素抗性的突变株的大肠杆菌产生ECoRI限制性内切核酸酶，并且确实产生从大肠杆菌突变体细胞获得的所述酶，而不通过提取程序破坏细胞。
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