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    Process for the continuous enzymatic change of water soluble .alpha.-ketocarboxylic acids into the corresponding amino acids
    7.
    发明授权
    Process for the continuous enzymatic change of water soluble .alpha.-ketocarboxylic acids into the corresponding amino acids 失效
    将水溶性α-酮羧酸连续酶促转化为相应氨基酸的方法

    公开(公告)号：US4304858A

    公开(公告)日：1981-12-08

    申请号：US172446

    申请日：1980-07-25

    申请人： Christian Wandrey ,  Rolf Wichmann ,  Wolfgang Leuchtenberger ,  Maria-Regina Kula ,  Andreas Buckmann

    发明人： Christian Wandrey ,  Rolf Wichmann ,  Wolfgang Leuchtenberger ,  Maria-Regina Kula ,  Andreas Buckmann

    IPC分类号： C07C229/06 ,  C12M1/34 ,  C12P1/00 ,  C12P13/04 ,  C12P13/06 ,  C12P19/36 ,  C12P13/08

    CPC分类号： C12P13/06 ,  C12P1/00 ,  C12P13/04 ,  C12P19/36 ,  Y10S435/813

    摘要： Water soluble .alpha.-ketocarboxylic acids are continuously converted in a membrane reactor into the corresponding aminoacids. The conversion takes place in the presence of a substrate specific dehydrogenase, of ammonium ions and of a nicotinamide-adenine-dinucleotide (NAD.sup.+ /NADH) enlarged in molecular weight through linkage to a water soluble polymer as coenzyme. Simultaneously NADH is regenerated continuously from NAD.sup.+ in presence of a formate dehydrogenase and from formate ion. The membrane must have a mean pore diameter of 1 to 3 nm. As coenzyme there is employed 0.1 to 10 mmol/l of NAD.sup.+ /NADH present bound to a polyoxyethylene having an average molecular weight between 500 and 50,000. There is continuously supplied to the reactor a substrate stream which contains 50 to 100% of the maximum amount soluble, but not over 2,000 mmol/l, of the reacting .alpha.-ketocarboxylic acid in the form of a water soluble salt, an ammonium ion in an amount about equimolar to the amount of substrate and 100 to 6,000 mmol/l of a formate. There is maintained over the membrane a differential pressure of 0.1 to 15 bar. There is continuously drawn off behind the membrane a filtrate stream containing the aminoacid formed.

    摘要翻译： 水溶性α-酮羧酸在膜反应器中连续转化成相应的氨基酸。 通过与作为辅酶的水溶性聚合物连接，在底物特异性脱氢酶，铵离子和烟酰胺 - 腺嘌呤二核苷酸（NAD + / NADH）存在下进行转化，分子量增加。 同时NADH在甲酸脱氢酶和甲酸根离子存在下从NAD +连续再生。 膜的平均孔径必须为1〜3nm。 作为辅酶，使用0.1〜10mmol / l的NAD + / NADH，其与平均分子量在500〜50,000的聚氧乙烯结合。 向反应器中连续供应底物流，其含有50〜100％最大量可溶性但不超过2,000mmol / l的水溶性盐，铵离子形式的α-酮羧酸 相当于底物量的等摩尔量和100-6,000mmol / l甲酸盐的量。 在膜上保持0.1至15巴的压差。 在膜的后面连续抽出含有形成的氨基酸的滤液流。

    Process for the continuous enzymatic conversion of alpha-hydrocarboxylic acids into the corresponding optically active alpha-aminocarboxylic acids
    8.
    发明授权
    Process for the continuous enzymatic conversion of alpha-hydrocarboxylic acids into the corresponding optically active alpha-aminocarboxylic acids 失效
    将α-加氢羧酸连续酶促转化成相应的光学活性α-氨基羧酸的方法

    公开(公告)号：US4782020A

    公开(公告)日：1988-11-01

    申请号：US83882

    申请日：1987-08-10

    申请人： Wolfgang Leuchtenberger ,  Christian Wandrey ,  Maria-Regina Kula

    发明人： Wolfgang Leuchtenberger ,  Christian Wandrey ,  Maria-Regina Kula

    IPC分类号： C12P13/04 ,  C12P13/06 ,  C12P13/12 ,  C12P41/00 ,  C12Q1/32

    CPC分类号： C12P13/06 ,  C12P13/04 ,  C12P13/12 ,  Y10S435/813

    摘要： .alpha.-Hydroxycarboxylic acids are continuously converted into the corresponding optically active .alpha.- aminocarboxylic acids. The conversion is carried out in a membrane reactor in the presence of nicotinamide-adenine dinucleotide increased in molecular weight by bonding to a water soluble high molecular weight material, a dehydrogenase specific for the .alpha.-hydroxycarboxylic acid, a dehydrogenase specific for the corresponding .alpha.-amino-carboxylic acid and ammonium ions. There is continuously supplied to the membrane reactor an aqueous solution of the .alpha.-hydroxycarboxylic acid to be reacted, a substantially lesser amount of the corresponding .alpha.-ketocarboxy lic acid, and an amount of ammonium ion at least equivalent to the .alpha.-hydroxycarboxylic acid to be reacted. There is maintained over the membrane a difference in pressure 1 and 15 bar. Behind the membrane, there is continuously drawn off a filtrate stream containing the .alpha.-aminocarboxylic acid formed.

    摘要翻译： α-羟基羧酸连续转化为相应的光学活性α-氨基羧酸。 在通过结合到水溶性高分子量材料的烟酰胺 - 腺嘌呤二核苷酸增加分子量的存在下，在膜反应器中进行转化，对于α-羟基羧酸是特异性的脱氢酶，对于相应的α- 氨基羧酸和铵离子。 向膜反应器中连续供应待反应的α-羟基羧酸的水溶液，相当少量的相应的α-酮羧酸的丙酸，以及一定量的至少等同于α-羟基羧酸的铵离子至 被反应。 在膜上保持压力1和15巴的差异。 在膜的后面，连续地抽出含有形成的α-氨基羧酸的滤液流。

    Process for the continuous enzymatic change of water soluble .alpha.-ketocarboxylic acids into the corresponding .alpha.-hydroxycarboxylic acids
    9.
    发明授权
    Process for the continuous enzymatic change of water soluble .alpha.-ketocarboxylic acids into the corresponding .alpha.-hydroxycarboxylic acids 失效
    将水溶性α-酮羧酸连续酶促改变成相应的α-羟基羧酸的方法

    公开(公告)号：US4326031A

    公开(公告)日：1982-04-20

    申请号：US172444

    申请日：1980-07-25

    申请人： Christian Wandrey ,  Rolf Wichmann ,  Wolfgang Leuchtenberger ,  Maria-Regina Kula ,  Andreas Buckmann

    发明人： Christian Wandrey ,  Rolf Wichmann ,  Wolfgang Leuchtenberger ,  Maria-Regina Kula ,  Andreas Buckmann

    IPC分类号： C12N11/02 ,  C12P1/00 ,  C12P7/42 ,  C12P7/56

    CPC分类号： C12P1/00 ,  C12P7/42 ,  C12P7/56

    摘要： Water soluble .alpha.-ketocarboxylic acids are continuously converted in a membrane reactor into the corresponding .alpha.-hydrocarboxylic acids. The conversion takes place in the presence of a substrate specific dehydrogenase and of a nicotinamide-adenine-dinucleotide (NAD.sup.+ /NADH) enlarged in molecular weight through linkage to a water soluble polymer as coenzyme. Simultaneously NADH is regenerated continuously from NAD.sup.+ in presence of a formate dehydrogenase and from formate ion. The membrane must have a mean pore diameter of 1 to 3 nm. As coenzyme there is employed 0.1 to 10 mmol/l of NAD.sup.+ /NADH present bound to a water soluble polymer having an average molecular weight between 500 and 50,000. There is continuously supplied to the reactor a substrate stream which contains 50 to 100% of the maximum amount soluble, but not over 2,000 mmol/l, of the reacting .alpha.-ketocarboxylic acid in the form of a water soluble salt and 100 to 6,000 mmol/l of a formate. There is maintained over the membrane a differential pressure of 0.1 to 15 bar. There is continuously drawn off behind the membrane a filtrate stream containing the .alpha.-hydroxy acid formed.

    摘要翻译： 水溶性α-酮羧酸在膜反应器中连续转化成相应的α-羟基羧酸。 通过与作为辅酶的水溶性聚合物连接，在底物特异性脱氢酶和分子量增大的烟酰胺 - 腺嘌呤二核苷酸（NAD + / NADH）的存在下进行转化。 同时NADH在甲酸脱氢酶和甲酸根离子存在下从NAD +连续再生。 膜的平均孔径必须为1〜3nm。 作为辅酶，使用0.1〜10mmol / l的NAD + / NADH，其与平均分子量在500〜50,000的水溶性聚合物结合。 向反应器中连续供应底物物流，其含有50〜100％最大量可溶性但不超过2,000mmol / l的水溶性盐形式的α-酮羧酸反应物和100至6,000mmol / l的甲酸盐。 在膜上保持0.1至15巴的压差。 在膜的后面连续地抽出含有形成的α-羟基酸的滤液流。