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    METHOD AND PREPARING GLUCAN AND MANNAN, GLUCAN PREPARATION AND MANNAN PREPARATION PRODUCED THEREBY AND USE THEREOF
    2.
    发明申请
    METHOD AND PREPARING GLUCAN AND MANNAN, GLUCAN PREPARATION AND MANNAN PREPARATION PRODUCED THEREBY AND USE THEREOF 有权
    方法和制备葡萄糖和甘露糖，葡萄糖的制备和制备及其制备及其使用

    公开(公告)号：US20110045545A1

    公开(公告)日：2011-02-24

    申请号：US12990206

    申请日：2008-12-31

    申请人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Yan Zhang

    发明人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Yan Zhang

    IPC分类号： C12P19/04

    CPC分类号： C12P19/04 ,  C08B37/0003 ,  C08B37/0087

    摘要： The present invention provides a method for extracting glucan and mannan from the cell wall of a microorganism. Specifically, the method of the present invention in one embodiment comprises the steps of: a) treating the cells of the microorganism with an alkaline protease and an mannanase; b) separating the mixture from step a) into a heavy phase and a light phase; c) drying the heavy phase obtained from step b), obtaining the glucan preparation; and d) drying the light phase obtained from step b), obtaining the mannan preparation. Optionally, in the step c), the heavy phase obtained from step b) may be treated sequentially with an alkali and an acid, and separated again into a heavy phase and a light phase. The heavy phase is dried, obtaining the glucan preparation. The present invention further relates to the glucan preparation and mannan preparation produced thereby, and the uses thereof.

    摘要翻译： 本发明提供了从微生物细胞壁提取葡聚糖和甘露聚糖的方法。 具体地说，本发明的一个实施方案包括以下步骤：a）用碱性蛋白酶和甘露聚糖酶处理微生物细胞; b）将混合物从步骤a）分离成重相和轻相; c）干燥从步骤b）获得的重相，得到葡聚糖制剂; 和d）干燥从步骤b）得到的轻相，得到甘露聚糖制剂。 任选地，在步骤c）中，可以用碱和酸依次处理从步骤b）获得的重相，并再次分离成重相和轻相。 将重相干燥，得到葡聚糖制剂。 本发明还涉及由此制备的葡聚糖制剂和甘露聚糖制剂及其用途。

    Method for preparing glucan and mannan, glucan preparation and mannan preparation produced thereby and use thereof
    3.
    发明授权
    Method for preparing glucan and mannan, glucan preparation and mannan preparation produced thereby and use thereof 有权
    由此制备葡聚糖和甘露聚糖，葡聚糖制剂和甘露聚糖制剂的方法及其用途

    公开(公告)号：US08679797B2

    公开(公告)日：2014-03-25

    申请号：US12990206

    申请日：2008-12-31

    申请人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Yan Zhang

    发明人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Yan Zhang

    IPC分类号： C12P19/04 ,  C12P19/00 ,  C12P1/04 ,  C12P1/02 ,  C12N1/06 ,  A01N43/04

    CPC分类号： C12P19/04 ,  C08B37/0003 ,  C08B37/0087

    摘要： The present invention provides a method for extracting glucan and mannan from the cell wall of a microorganism. Specifically, the method of the present invention in one embodiment comprises the steps of: a) treating the cells of the microorganism with an alkaline protease and an mannanase; b) separating the mixture from step a) into a heavy phase and a light phase; c) drying the heavy phase obtained from step b), obtaining the glucan preparation; and d) drying the light phase obtained from step b), obtaining the mannan preparation. Optionally, in the step c), the heavy phase obtained from step b) may be treated sequentially with an alkali and an acid, and separated again into a heavy phase and a light phase. The heavy phase is dried, obtaining the glucan preparation. The present invention further relates to the glucan preparation and mannan preparation produced thereby, and the uses thereof.

    摘要翻译： 本发明提供了从微生物细胞壁提取葡聚糖和甘露聚糖的方法。 具体地说，本发明的一个实施方案包括以下步骤：a）用碱性蛋白酶和甘露聚糖酶处理微生物细胞; b）将混合物从步骤a）分离成重相和轻相; c）干燥从步骤b）获得的重相，得到葡聚糖制剂; 和d）干燥从步骤b）得到的轻相，得到甘露聚糖制剂。 任选地，在步骤c）中，可以用碱和酸依次处理从步骤b）获得的重相，并再次分离成重相和轻相。 将重相干燥，得到葡聚糖制剂。 本发明还涉及由此制备的葡聚糖制剂和甘露聚糖制剂及其用途。

    BIOTOXIN SORBENT AND METHOD FOR PREPARING THE SAME
    5.
    发明申请
    BIOTOXIN SORBENT AND METHOD FOR PREPARING THE SAME 审中-公开
    生物素SORBENT及其制备方法

    公开(公告)号：US20100189871A1

    公开(公告)日：2010-07-29

    申请号：US12668564

    申请日：2008-08-06

    申请人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Bin Tan ,  Jinlin Zhu

    发明人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Bin Tan ,  Jinlin Zhu

    IPC分类号： A23K1/175 ,  C12P19/04 ,  B01J20/12 ,  B01J20/22

    CPC分类号： B01J20/28004 ,  A23K20/163 ,  A23K20/28 ,  A23K50/10 ,  A23K50/70 ,  A23K50/80 ,  B01J20/12 ,  B01J20/16 ,  B01J20/24 ,  B01J2220/46

    摘要： The present invention provides a combination used for absorbing toxins in feed, comprising 1-10% by weight of clay and 90-99% by weight of yeast manna oligosaccharide, wherein the yeast manna oligosaccharide is extract of yeast cell wall which is separated from Saccharomyces cerevisiae. The biotoxin sorbent product can be used to feed any animals, including livestock, fowls, marine lives and ruminants. When mixing with feed, owing to its strong toxin adsorption capability, this absorbent may reduce the amount of toxins entering bodies of animals, thereby improving productivity and health of animals, reducing diseases caused by toxins.

    摘要翻译： 本发明提供了用于吸收饲料中毒素的组合物，其包含1-10重量％的粘土和90-99重量％的酵母甘露寡糖，其中所述酵母甘露寡糖是酵母细胞壁的提取物，其从酵母菌 啤酒酵母 生物毒素吸附剂产品可用于饲养任何动物，包括牲畜，家禽，海洋生物和反刍动物。 当与饲料混合时，由于其强的毒素吸附能力，该吸收剂可以减少进入动物体内的毒素的量，从而提高动物的生产力和健康，减少由毒素引起的疾病。

    Composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials
    6.
    发明授权
    Composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials 有权
    复合酵母适用于含糖原料的高浓度酒精发酵

    公开(公告)号：US08263374B2

    公开(公告)日：2012-09-11

    申请号：US12668578

    申请日：2008-07-30

    申请人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Zhijun Li ,  Daiwu Liu

    发明人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Zhijun Li ,  Daiwu Liu

    IPC分类号： C12P7/14 ,  C12P7/06 ,  C12N1/00 ,  C12N1/12 ,  A01N63/04

    CPC分类号： C12P39/00 ,  C12N1/16 ,  C12P7/14 ,  Y02E50/17

    摘要： The present invention relates to a composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials, characterizing in that the composite yeast comprises any kind of dried yeast selected from Brewers yeast Saccharomyces cerevisiae Hansen of Saccharomyces cerevisiae, grape wine yeast Saccharomyces uvarum Beijerinek, and nutritious materials which are necessary for yeast growth, the nutritious materials include: the dried yeast 40-70 parts by weight, a nitrogen source 20-40 parts by weight, a phosphorous source 5-10 parts by weight, an other inorganic salt 2.5-5 parts by weight, a trace vitamin 1-2.5 parts by weight and a bacteriostatic 0.5-1.2 parts by weight. The present invention further relates to a method for preparation of the composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials. Using the composite yeast of the present invention to proceed with sugar fermentation can increase fermentation alcoholicity, decrease residual sugar content, and allows the final alcoholicity of standard raw material, such as sucrose to attain to 14.5-15.5% v/v, and the amount of the residual reducing sugar in the fermentation mash is 0-0.1 wt %.

    摘要翻译： 本发明涉及适用于含糖原料的高浓度酒精发酵的复合酵母，其特征在于复合酵母包含任何种类的干酵母，其选自酿酒酵母酿酒酵母酿酒酵母Hansen，酿酒酵母酿酒酵母Beijerinek 和酵母生长所必需的营养物质，营养物质包括：干燥酵母40-70重量份，氮源20-40重量份，磷源5-10重量份，其它无机盐 2.5-5重量份，微量维生素1-2.5重量份，抑菌剂0.5-1.2重量份。 本发明还涉及一种适用于含糖原料的高浓度酒精发酵的复合酵母的制备方法。 使用本发明的复合酵母进行糖发酵可以提高发酵酒精度，降低残留糖含量，并允许标准原料如蔗糖的最终酒精度达到14.5-15.5％v / v， 的发酵麦芽汁中的残留还原糖为0-0.1重量％。

    METHOD FOR PRODUCING CELLULOSIC ETHANOL
    7.
    发明申请
    METHOD FOR PRODUCING CELLULOSIC ETHANOL 审中-公开
    生产纤维素乙醇的方法

    公开(公告)号：US20110171710A1

    公开(公告)日：2011-07-14

    申请号：US13054851

    申请日：2009-09-27

    申请人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Zhijun Li ,  Daiwu Liu

    发明人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Zhijun Li ,  Daiwu Liu

    IPC分类号： C12P7/10

    CPC分类号： C12P7/10 ,  C12P7/065 ,  Y02E50/16 ,  Y02E50/17

    摘要： A method for producing cellulosic ethanol is disclosed, which comprises the following steps: adding the medium containing the source materials of cellulose and/or hemicellulose to fermentation reaction vessel; adding cellulase to fermentation reaction vessel, and inoculating Candida lusitaniae; running simultaneous saccharification fermentation (SSFs) with the combined action of cellulose and Candida lusitaniae, and obtaining cellulosic ethanol by separation. Candida lusitaniae used in SSFs in present invention has higher ethanol tolerance and a higher ethanol production rate, it can use cellulose and hemicellulose as source materials and effectively produce cellulosic ethanol with cellulase in SSFs.

    摘要翻译： 公开了一种生产纤维素乙醇的方法，包括以下步骤：向发酵反应容器中加入含有纤维素和/或半纤维素原料的培养基; 向发酵反应容器中加入纤维素酶，接种假丝酵母; 通过纤维素和假丝酵母（Candida lusitaniae）的联合作用同时进行糖化发酵（SSFs），并通过分离获得纤维素乙醇。 本发明SSF中使用的假丝酵母具有较高的乙醇耐受性和较高的乙醇生产率，可以使用纤维素和半纤维素作为原料，并在SSF中有效地生产纤维素酶纤维素乙醇。

    Yeast used for pizza and its production method
    9.
    发明申请
    Yeast used for pizza and its production method 审中-公开
    酵母用于比萨饼及其生产方法

    公开(公告)号：US20100248332A1

    公开(公告)日：2010-09-30

    申请号：US12733942

    申请日：2009-05-18

    申请人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Jinbao Kuang ,  Jinjun Shen

    发明人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Jinbao Kuang ,  Jinjun Shen

    IPC分类号： C12N1/16

    CPC分类号： C12N1/18 ,  A21D2/16 ,  A21D8/047 ,  A21D13/41 ,  A23L33/14 ,  C12R1/865

    摘要： A yeast used for pizza and its production method are provided. The yeast is dry yeast, and the amount of CO2 produced in the dough at 30° C. during 60 minutes is 250˜450 mL CO2/h, wherein the dough contains those ingredients as following: 280 g flour, 1.43% salt by weigh of flour, 4 g pure dry yeast and appropriate content water. The production method includes the following steps: A) one-step fermenting; B) drying: in which, after the one-step fermenting without further fermenting; before or during the drying step, adding the emulsifier. Since adapting the first seed yeast as commercial yeast, the fermenter procedure is omitted, the process is simplified, the cost is reduced, and the yeast produced meets the property requirements of the yeast used for pizza.

    摘要翻译： 提供了用于比萨饼的酵母及其制备方法。 酵母是干酵母，在60分钟内在30℃下生产的二氧化碳的量为250〜450mL CO 2 / h，其中面团含有以下成分：280g面粉，1.43重量％的盐 的面粉，4克纯干酵母和适量含水。 生产方法包括以下步骤：A）一步发酵; B）干燥：其中一步发酵无需进一步发酵; 在干燥步骤之前或期间，加入乳化剂。 由于将第一种子酵母适应为商业酵母，所以省略了发酵程序，简化了过程，降低了成本，并且所生产的酵母符合比萨饼用酵母的性能要求。

    Method for processing lignocellulose material
    10.
    发明授权
    Method for processing lignocellulose material 有权
    木质纤维素材料的处理方法

    公开(公告)号：US08828187B2

    公开(公告)日：2014-09-09

    申请号：US13445894

    申请日：2012-04-12

    申请人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Daiwu Liu ,  Jincheng Lei ,  Zhijun Li

    发明人： Xuefeng Yu ,  Zhihong Li ,  Minghua Yu ,  Juan Yao ,  Daiwu Liu ,  Jincheng Lei ,  Zhijun Li

    IPC分类号： D21C1/00 ,  C12P19/14

    CPC分类号： C12P19/14 ,  C12P19/02 ,  Y02E50/16

    摘要： A method for processing a lignocellulose material, comprises the following steps: (1) crushing and sieving the lignocellulose material, and collecting granules with a particle size of between 0.08 and 0.1 mm; (2) mixing the granules obtained in step (1) with water, and dispersing through a colloid mill to yield a suspension with a particle size of 40-80 μm; (3) homogenizing the suspension obtained in the step (2) under high pressure to have a particle size of between 10 and 40 μm; and (4) buffering the suspension obtained in the step (3) with a buffer solution of sodium acetate and acetic-acid, adding cellulase, β-glucosidase, and xylanase, and performing zymolysis for 36-72 hours.

    摘要翻译： 一种处理木质纤维素材料的方法，包括以下步骤：（1）对木质纤维素材料进行破碎筛分，并收集粒度在0.08至0.1mm之间的颗粒; （2）将步骤（1）中获得的颗粒与水混合，并通过胶体磨分散，得到粒径为40-80μm的悬浮液; （3）在高压下使步骤（2）中得到的悬浮液均匀化，使其粒径在10〜40μm之间; 和（4）用乙酸钠和乙酸缓冲溶液缓冲步骤（3）获得的悬浮液，加入纤维素酶，葡糖苷酶和木聚糖酶，并进行酶解36-72小时。