公开(公告)号：US5824522A

公开(公告)日：1998-10-20

申请号：US294871

申请日：1994-08-22

申请人： Yasuhiro Ikenaka ,  Hirokazu Nanba ,  Masayuki Takano ,  Kazuyoshi Yajima ,  Yukio Yamada ,  Satomi Takahashi ,  Kazuma Okubo ,  Kazuhiko Yamada ,  Yoshiro Hiraishi

发明人： Yasuhiro Ikenaka ,  Hirokazu Nanba ,  Masayuki Takano ,  Kazuyoshi Yajima ,  Yukio Yamada ,  Satomi Takahashi ,  Kazuma Okubo ,  Kazuhiko Yamada ,  Yoshiro Hiraishi

IPC分类号： C12N9/80 ,  C12N9/96 ,  C12N11/02 ,  C12N11/08 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/22 ,  C12P41/00 ,  C12N1/20 ,  C12N9/14 ,  C12N11/00

CPC分类号： C12N11/02 ,  C12N11/08 ,  C12N9/80 ,  C12N9/96 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/22 ,  C12P41/009 ,  Y10S435/849 ,  Y10S435/874

摘要： Decarbamylases are provided capable of producing D-.alpha.-amino acids by hydrolysis of N-carbamyl-D-.alpha.-amino acids. A source of the decarbamylases is recombinant microorganisms produced by gene manipulation methods. Decarbamylases having improved thermostability can be obtained in which amino acids at a thermostability-related site of a natural decarbamylase have been replaced with other amino acids by mutating a DNA fragment encoding the natural decarbamylase. Recombinant DNA is obtained from a vector DNA and a DNA fragment encoding a natural decarbamylase where the nucleic acid sequence encoding an amino acid at a thermostability-related site is replaced with a nucleic acid sequence encoding another amino acid. The recombinant DNA is used to produce transformants that produce thermostable decarbamylases. The decarbamylases may be immobilized in purified, partially-purified or crude form or in the form of decarbamylase-containing microbial cells on a support such as a polymer having ion exchange groups or covalent bonding groups. The presence of an antioxidant such as dithiothreitol results in an immobilized decarbamylase preparation that can be repeatedly used 30 times or more.

摘要翻译： 提供的脱甲酰氨基酶能够通过N-氨基甲酰基-D-α-氨基酸的水解产生D-氨基酸。 脱氨基甲酰酶的来源是通过基因操作方法产生的重组微生物。 通过突变编码天然脱氨基甲酰酶的DNA片段，可以得到具有改善的热稳定性的脱甲酰氨基磷酸酶，其中天然脱氨基甲酰酶的热稳定性相关部位的氨基酸已被其它氨基酸替代。 从载体DNA和编码天然脱氨基甲酰酶的DNA片段获得重组DNA，其中将编码热稳定性相关部位的氨基酸的核酸序列替换为编码另一氨基酸的核酸序列。 重组DNA用于产生产生热稳定性脱氨基甲酰酶的转化体。 脱氨基甲酰酶可以固定在纯化的，部分纯化的或粗制的形式中，或以含脱氨基甲酰酶的微生物细胞的形式固定在载体上，例如具有离子交换基团或共价键合基团的聚合物。 抗氧化剂如二硫苏糖醇的存在导致可重复使用30次以上的固定化脱氨基甲酰酶制剂。

公开(公告)号：US20060149098A1

公开(公告)日：2006-07-06

申请号：US11276306

申请日：2006-02-23

申请人： Masafumi FUKAE ,  Yoshiro Hiraishi

发明人： Masafumi FUKAE ,  Yoshiro Hiraishi

IPC分类号： C07C61/12

CPC分类号： C07C51/02 ,  C07C51/43 ,  C07C2602/28 ,  C07C59/46

摘要： The invention aims at producing high-purity crystals of a hydroxycarboxylic acid represented by the following formula (1): in high yield. Provided are a method for crystallization of a compound (1) which comprises acidifying a mixture of a solution of an alkali salt of the compound (1) and an organic solvent, and a method for crystallization of compound (1) by mixing a solution of the compound (1) in a water-miscible good solvent with water, in which a slurry with a necessary suspension amount of the compound (1) for inhibiting oil formation and scaling is prepared in advance and then a main crystallization is carried out in the presence of said slurry.

摘要翻译： 本发明旨在以高产率制备由下式（1）表示的羟基羧酸的高纯度晶体。 提供化合物（1）的结晶方法，其包括酸化化合物（1）的碱金属盐和有机溶剂的溶液的混合物，以及通过将化合物（1）的溶液 将与化合物（1）在水混溶性好的溶剂中与水一起预先制备具有用于抑制油形成和结垢的化合物（1）所需悬浮液量的浆料，然后进行主结晶 所述浆料的存在。

公开(公告)号：US20050075508A1

公开(公告)日：2005-04-07

申请号：US10362460

申请日：2001-09-07

申请人： Masafumi Fukae ,  Yoshiro Hiraishi

发明人： Masafumi Fukae ,  Yoshiro Hiraishi

IPC分类号： C07C51/02 ,  C07C51/43 ,  C07D309/30

CPC分类号： C07C51/02 ,  C07C51/43 ,  C07C2602/28 ,  C07C59/46

摘要： The invention aims at producing high-purity crystals of a hydroxycarboxylic acid represented by the following formula (1): in high yield. Provided are a method for crystallization of a compound (1) which comprises acidifying a mixture of a solution of an alkali salt of the compound (1) and an organic solvent, and a method for crystallization of compound (1) by mixing a solution of the compound (1) in a water-miscible good solvent with water, in which a slurry with a necessary suspension amount of the compound (1) for inhibiting oil formation and scaling is prepared in advance and then a main crystallization is carried out in the presence of said slurry.

摘要翻译： 本发明旨在以高产率制备由下式（1）表示的羟基羧酸的高纯度晶体。 提供化合物（1）的结晶方法，其包括酸化化合物（1）的碱金属盐和有机溶剂的溶液的混合物，以及通过将化合物（1）的溶液 将与化合物（1）在水混溶性好的溶剂中与水一起预先制备具有用于抑制油形成和结垢的化合物（1）所需悬浮液量的浆料，然后进行主结晶 所述浆料的存在。

公开(公告)号：US07034180B2

公开(公告)日：2006-04-25

申请号：US10362460

申请日：2001-09-07

申请人： Masafumi Fukae ,  Yoshiro Hiraishi

发明人： Masafumi Fukae ,  Yoshiro Hiraishi

IPC分类号： C07C61/12

CPC分类号： C07C51/02 ,  C07C51/43 ,  C07C2602/28 ,  C07C59/46

摘要： The invention aims at producing high-purity crystals of a hydroxycarboxylic acid represented by the following formula (1): in high yield. Provided are a method for crystallization of a compound (1) which comprises acidifying a mixture of a solution of an alkali salt of the compound (1) and an organic solvent, and a method for crystallization of compound (1) by mixing a solution of the compound (1) in a water-miscible good solvent with water, in which a slurry with a necessary suspension amount of the compound (1) for inhibiting oil formation and scaling is prepared in advance and then a main crystallization is carried out in the presence of said slurry.

摘要翻译： 本发明旨在以高产率制备由下式（1）表示的羟基羧酸的高纯度晶体。 提供化合物（1）的结晶方法，其包括酸化化合物（1）的碱金属盐和有机溶剂的溶液的混合物，以及通过将化合物（1）的溶液 将与化合物（1）在水混溶性好的溶剂中与水一起预先制备具有用于抑制油形成和结垢的化合物（1）所需悬浮液量的浆料，然后进行主结晶 所述浆料的存在。

公开(公告)号：US07323592B2

公开(公告)日：2008-01-29

申请号：US11276306

申请日：2006-02-23

申请人： Masafumi Fukae ,  Yoshiro Hiraishi

发明人： Masafumi Fukae ,  Yoshiro Hiraishi

IPC分类号： C07C61/12

CPC分类号： C07C51/02 ,  C07C51/43 ,  C07C2602/28 ,  C07C59/46

摘要： The invention aims at producing high-purity crystals of a hydroxycarboxylic acid represented by the following formula (1): in high yield. Provided are a method for crystallization of a compound (1) which comprises acidifying a mixture of a solution of an alkali salt of the compound (1) and an organic solvent, and a method for crystallization of compound (1) by mixing a solution of the compound (1) in a water-miscible good solvent with water, in which a slurry with a necessary suspension amount of the compound (1) for inhibiting oil formation and scaling is prepared in advance and then a main crystallization is carried out in the presence of said slurry.

摘要翻译： 本发明旨在以高产率制备由下式（1）表示的羟基羧酸的高纯度晶体。 提供化合物（1）的结晶方法，其包括酸化化合物（1）的碱金属盐和有机溶剂的溶液的混合物，以及通过将化合物（1）的溶液 将与化合物（1）在水混溶性好的溶剂中与水一起预先制备具有用于抑制油形成和结垢的化合物（1）所需悬浮液量的浆料，然后进行主结晶 所述浆料的存在。