公开(公告)号：US20180273911A1

公开(公告)日：2018-09-27

申请号：US15764691

申请日：2016-09-29

Applicant: RAMOT AT TEL-AVIV UNIVERSITY LTD. ,  YEDA RESEARCH AND DEVELOPMENT CO. LTD. ,  SYNVACCINE LTD.

Inventor： Tamir TULLER ,  Eli GOZ ,  Shimshi ATAR ,  Hadas ZUR

IPC: C12N7/00 ,  A61K39/12

CPC classification number: C12N7/00 ,  A61K35/76 ,  A61K39/12 ,  A61K2039/5254 ,  C12N2770/24121 ,  C12N2770/24162 ,  G16B99/00 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/391 ,  Y02A50/465

Abstract: Attenuated viruses and methods of designing them are disclosed. In one embodiment, there is disclosed an attenuated form of a virulent virus comprising an RNA encoding a viral protein or a nucleic acid sequence transcribable to said RNA, wherein the folding energy or structure of the RNA is changed at positions of evolutionarily conserved RNA structures with respect to that of said RNA encoding said viral protein in the virulent virus so as to bring about attenuation of the virus.

公开(公告)号：US20220010287A1

公开(公告)日：2022-01-13

申请号：US17467265

申请日：2021-09-05

Applicant: RAMOT AT TEL-AVIV UNIVERSITY LTD. ,  YEDA RESEARCH AND DEVELOPMENT CO. LTD. ,  SYNVACCINE LTD.

Inventor： Tamir TULLER ,  Eli GOZ ,  Shimshi ATAR ,  Hadas ZUR

IPC: C12N7/00 ,  G16B99/00 ,  C12N7/04 ,  G16B15/10 ,  A61K39/12

Abstract: Attenuated viruses and methods of designing them are disclosed. In one embodiment, there is disclosed an attenuated form of a virulent virus comprising an RNA encoding a viral protein or a nucleic acid sequence transcribable to said RNA, wherein the folding energy or structure of the RNA is changed at positions of evolutionarily conserved RNA structures with respect to that of said RNA encoding said viral protein in the virulent virus so as to bring about attenuation of the virus.

公开(公告)号：US20160152991A1

公开(公告)日：2016-06-02

申请号：US15040018

申请日：2016-02-10

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yitzhak PILPEL ,  Tamir TULLER ,  Sivan NAVON ,  Menachem RUBINSTEIN

IPC: C12N15/67 ,  C12P21/02

CPC classification number: C12N15/67 ,  C07K14/505 ,  C07K14/555 ,  C07K14/59 ,  C07K14/61 ,  C07K14/62 ,  C07K14/705 ,  C07K14/755 ,  C12N9/2402 ,  C12N9/2465 ,  C12P21/02

Abstract: Isolated polynucleotides are disclosed which increase the efficiency of gene expression in a heterologous cell. The polynucleotide sequences which encode polypeptides are adapted such that the average rate of translation of the first at least about 30 amino acids is slower by at least two fold than the average rate of translation of the remaining amino acids of the polypeptide.

Abstract translation: 公开了分离的多核苷酸，其提高异源细胞中基因表达的效率。 改编编码多肽的多核苷酸序列使得第一至少约30个氨基酸的平均翻译速率比多肽的剩余氨基酸的平均翻译速度慢至少两倍。