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1.发明申请公开(公告)号：US20070003929A1
公开(公告)日：2007-01-04
申请号：US10538941
申请日：2003-12-12
CPC分类号： C12Q1/6827 , Y02A90/26 , C12Q2537/113 , C12Q2521/514 , C12Q2563/131 , C12Q2521/301 , C12Q2521/307
摘要： The present invention provides a method for identifying, analyzing and/or cloning nucleic acid isoforms comprising the steps of: preparing at least two nucleic acid isoforms, complementary to each other, hybridizing the at least two complementary nucleic acid isoforms and forming double strand RNA/RNA or DNA/DNA hybrids comprising unpaired regions; recovering the RNA/RNA or DNA/DNA hybrids comprising unpaired regions from not hybridized nucleic acids and from nucleic acids not comprising unpaired regions; and identifying, analyzing and/or cloning the recovered nucleic acid fragment comprising unpaired regions.
摘要翻译： 本发明提供了用于鉴定，分析和/或克隆核酸同种型的方法，其包括以下步骤：制备彼此互补的至少两个核酸同种型，将所述至少两种互补核酸同种型杂交并形成双链RNA / 包含不成对区域的RNA或DNA / DNA杂交体; 从未杂交的核酸和不包含未配对区域的核酸中回收包含不成对区域的RNA / RNA或DNA / DNA杂交体; 以及鉴定，分析和/或克隆包含不成对区域的回收的核酸片段。
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公开(公告)号：US20070178482A1
公开(公告)日：2007-08-02
申请号：US11462939
申请日：2006-08-07
申请人： Alexander Lezhava , Yuko Shibata , Matthias Harbers , Toshizo Hayashi , Yoshihide Hayashizaki , Piero Carninci
发明人： Alexander Lezhava , Yuko Shibata , Matthias Harbers , Toshizo Hayashi , Yoshihide Hayashizaki , Piero Carninci
CPC分类号： C12Q1/6806 , C12N15/1003 , C12Q2522/101 , C12Q2521/301 , C12Q2521/325 , C12Q2527/143
摘要： The invention is a method and a kit for preparing single-stranded DNA from double-stranded DNA and the purification of single-stranded DNA derived from double-stranded DNA. A single-stranded-DNA binding substance is used in combination with a double-strand-specific endonuclease for the removal of undesired double-stranded DNA from a single-stranded DNA preparation and for other related purposes.
摘要翻译： 本发明是用于从双链DNA制备单链DNA并纯化由双链DNA衍生的单链DNA的方法和试剂盒。 单链DNA结合物质与双链特异性内切核酸酶组合使用，用于从单链DNA制备物中除去不想要的双链DNA并用于其它相关目的。
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公开(公告)号：US20080108804A1
公开(公告)日：2008-05-08
申请号：US11591682
申请日：2006-11-02
IPC分类号： C07H21/04
CPC分类号： C07H21/04 , C12N15/1096 , C12Q1/686 , C12Q2521/107 , C12Q2533/107
摘要： A method is disclosed for the modification of an end of RNA molecules and the use of such modified RNA molecules in cDNA synthesis for the purpose of cloning, detection, sequencing, and amplification of parts of the RNAs, the entire RNAs, or any cDNAs derived from such modified RNAs. The invention relates further to the amplification and the identification of nucleic acid molecules for the purpose of single molecule detection and/or high-throughput sequencing. In addition, a method is provided for the preparation of pooled samples that contains molecules each of which is marked by an “Identifier Sequence” for its origin. The invention facilitates studies on biological systems and analysis of genes expressed therein.
摘要翻译： 公开了用于修饰RNA分子末端的方法以及在cDNA合成中使用这些修饰的RNA分子，以克隆，检测，测序和扩增部分RNA，整个RNA或衍生的任何cDNA 来自这些修饰的RNA。 本发明进一步涉及用于单分子检测和/或高通量测序目的的核酸分子的扩增和鉴定。 此外，提供了一种用于制备包含分子的合并样品的方法，每个分子由其起源的“标识序列”标记。 本发明有助于对生物系统的研究和其中表达的基因的分析。
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公开(公告)号：US20100035249A1
公开(公告)日：2010-02-11
申请号：US12186009
申请日：2008-08-05
申请人： Yoshihide Hayashizaki , Piero Carninci , Mutsumi Katayama , Shintaro Katayama , Masayoshi Itoh , Matthias Harbers
发明人： Yoshihide Hayashizaki , Piero Carninci , Mutsumi Katayama , Shintaro Katayama , Masayoshi Itoh , Matthias Harbers
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6834 , C12Q1/6874 , C12Q2535/101 , C12Q2565/537 , C12Q2525/155
摘要： The present invention provides methods for the sequencing of all RNA species within an RNA sample, such as the RNA content obtained from a cell, a tissue, a living organism, or from an artificial source. RNA molecules within the samples are labeled in a RNA-specific manner prior to immobilization on a solid support. One label is used to mark the location of the RNA molecule on the solid support, whereas the second label is used to mark selectively the S′ end of full-length mRNA molecules. RNA molecules are sequenced while being bound to the solid support in one or more sequencing reactions, and sequences of individual RNA molecules can be forwarded to computational analysis for assembling sequence information from individual sequencing reads obtained from the same location on the solid support. Not only unsupervised expression profiling on a genome-wide scale, but also the direct analysis of RNA-RNA interactions become possible as revealed by the analysis of the sequencing information obtained along with genomic information.
摘要翻译： 本发明提供了用于对RNA样品中所有RNA物种进行测序的方法，例如从细胞，组织，活生物体或人造来源获得的RNA含量。 在固定在固体支持物上之前，将样品中的RNA分子以RNA特异性方式标记。 一个标签用于标记RNA分子在固体支持物上的位置，而第二个标记用于选择性标记全长mRNA分子的S'末端。 RNA分子在一个或多个测序反应中与固体支持物结合时进行测序，并且可将单个RNA分子的序列转发到计算分析，以从固体支持物上的相同位置获得的单独测序读数组装序列信息。 不仅在基因组范围内无监督的表达谱，而且通过分析与基因组信息一起获得的测序信息显示，RNA-RNA相互作用的直接分析也是可能的。
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5.发明申请Method of utilizing the 5'end of transcribed nucleic acid regions for cloning and analysis 审中-公开利用转录核酸区域的5'端克隆和分析的方法公开(公告)号：US20050250100A1
公开(公告)日：2005-11-10
申请号：US10517544
申请日：2003-06-12
CPC分类号： C12N15/1093 , C12N15/1096
摘要： A method is disclosed for obtaining the 5′ends of transcribed regions from a plurality of nucleic acid fragments obtained from biological materials or synthetic pools. DNA fragments encoding the 5′ends are enriched for their individual analysis or for the analysis of concatemers thereof. The sequence information derived from 5′ ends can be used for characterization and cloning of the transcriptome.
摘要翻译： 公开了从生物材料或合成池获得的多个核酸片段获得转录区的5'端的方法。 丰富了编码5'端的DNA片段进行单独分析或分析其并行物。 从5'末端得到的序列信息可用于表达和克隆转录组。
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公开(公告)号：US20110059869A1
公开(公告)日：2011-03-10
申请号：US12919594
申请日：2009-02-27
CPC分类号： C12N9/96 , C12Q1/6834 , C12Q1/686 , C12Q1/6869 , C12Q2527/127 , C12Q2527/125 , C12Q2521/543
摘要： An object of the invention is to provide a method for increasing enzymatic reactivity to a target substance immobilized on a support; and a method for reducing or suppressing an inhibitory effect of a support on enzymatic reaction. The above object is achieved by a method for increasing enzymatic reactivity to a target substance immobilized on a support by allowing at least one substance selected from the group consisting of saccharides, amino acids, polyhydric alcohols and derivatives thereof to exist; and a method for reducing or suppressing an inhibitory effect of a support immobilized with a target substance on enzymatic reactivity to the target substance by allowing at least one substance selected from the group consisting of saccharides, amino acids, polyhydric alcohols and derivatives thereof to exist.
摘要翻译： 本发明的目的是提供一种增加对固定在载体上的目标物质的酶反应性的方法; 以及减少或抑制载体对酶促反应的抑制作用的方法。 上述目的通过使至少一种选自由糖，氨基酸，多元醇及其衍生物组成的组中选出的物质存在而增加对固定在载体上的目标物质的酶反应性的方法来实现。 以及通过使至少一种选自由糖，氨基酸，多元醇及其衍生物组成的组的物质存在的方式，减少或抑制固定有靶物质的载体对目标物质的酶反应性的抑制作用。
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公开(公告)号：US20070042363A1
公开(公告)日：2007-02-22
申请号：US10509727
申请日：2003-01-22
IPC分类号： C12Q1/68 , C07H21/04 , C12P21/06 , C07K14/705
CPC分类号： C07K14/47
摘要： A novel protein which binds with tumor necrosis factor receptor associated factor 2, TRAF2, as well as a nucleic acid coding for the protein is disclosed. From a mouse cDNA library, a cDNA coding for a novel protein which binds with TRAF2 was discovered by mammalian two-hybrid assay, the cDNA was sequenced, the protein encoded by the cDNA was produced, and the fact that the protein binds with TRAF2 was experimentally confirmed.
摘要翻译： 公开了与肿瘤坏死因子受体相关因子2，TRAF2结合的新型蛋白质以及编码该蛋白质的核酸。 从小鼠cDNA文库中，通过哺乳动物双杂交测定发现编码与TRAF2结合的新蛋白质的cDNA，对cDNA进行测序，产生由cDNA编码的蛋白质，并且蛋白质与TRAF2结合的事实为 实验证实。
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公开(公告)号：US20080096255A1
公开(公告)日：2008-04-24
申请号：US11571562
申请日：2004-07-02
申请人： Matthias Harbers , Yuko Shibata
发明人： Matthias Harbers , Yuko Shibata
IPC分类号： C12P19/34
CPC分类号： C12N15/1096
摘要： Means to circulate any nucleic acid molecule and to obtain from such circular nucleic acid molecules fragments that mark both ends of the initial nucleic acid molecule are provided. Means of high value to studies including, but not limited to, expression profiling, splicing, promoter identification, identification of genetic elements, and beyond, which are essential components of commercial applications and services including, but not limited to, drug development, diagnostics, or forensic studies are also provided.
摘要翻译： 提供了循环任何核酸分子并从这种环状核酸分子获得标记起始核酸分子两端的片段的手段。 对研究具有高价值的手段，包括但不限于表达谱，拼接，启动子鉴定，遗传因子鉴定等等，这些是商业应用和服务的重要组成部分，包括但不限于药物开发，诊断， 还提供法医学研究。
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