公开(公告)号：US07235357B2

公开(公告)日：2007-06-26

申请号：US10179395

申请日：2002-06-26

申请人： Yoshihide Iwaki ,  Hiroshi Shinoki ,  Osamu Seshimoto ,  Kouki Nakamura

发明人： Yoshihide Iwaki ,  Hiroshi Shinoki ,  Osamu Seshimoto ,  Kouki Nakamura

IPC分类号： C12Q1/68 ,  C12P13/16 ,  C07C61/00 ,  C07C229/00

CPC分类号： G01N33/54393 ,  C12Q1/6837 ,  G01N21/6428 ,  C12Q2549/125

摘要： The present invention provides a method for detecting fluorescence by using a solid support to which a probe molecule to be detected is fixed, wherein background is reduced by using a quenching agent. By using present invention, detection sensitivity of a DNA chip can be increased and stable data can be obtained.

摘要翻译： 本发明提供一种通过使用待固定探针分子的固体支持物来检测荧光的方法，其中通过使用淬灭剂降低背景。 通过使用本发明，可以提高DNA芯片的检测灵敏度，并获得稳定的数据。

公开(公告)号：US07067272B2

公开(公告)日：2006-06-27

申请号：US10239499

申请日：2001-03-23

申请人： Ryoichi Nemori ,  Masayoshi Yamamoto ,  Kouki Nakamura ,  Yasunori Okada

发明人： Ryoichi Nemori ,  Masayoshi Yamamoto ,  Kouki Nakamura ,  Yasunori Okada

IPC分类号： C12Q1/37

CPC分类号： C12Q1/37 ,  G01N2333/96486

摘要： A thin membrane for measuring protease activity, which is formed on a support and, which comprises one or more kinds of substances selected from the group consisting of a transferrin derivative and an albumin derivative and is crosslinked and/or substantially water-insoluble. The membrane enables selective measurement of protease activity of a particular class of protease such as matrix metalloproteinase 7.

摘要翻译： 用于测量蛋白酶活性的薄膜，其形成在载体上，并且包含一种或多种选自转铁蛋白衍生物和白蛋白衍生物的物质，并且是交联和/或基本上不溶于水的。 该膜能够选择性地测量特定类别的蛋白酶如蛋白酶的蛋白酶活性，例如基质金属蛋白酶7。

公开(公告)号：US20050112770A1

公开(公告)日：2005-05-26

申请号：US10366449

申请日：2003-02-14

申请人： Kouki Nakamura ,  Kazuya Takeuchi

发明人： Kouki Nakamura ,  Kazuya Takeuchi

IPC分类号： C09B23/02 ,  C12Q1/68 ,  G01N21/64

CPC分类号： C09B23/02 ,  C09B23/04 ,  C09B23/06 ,  C09B23/083 ,  C09B69/001 ,  C12Q1/6816 ,  Y10T436/143333 ,  C12Q2565/107

摘要： A method for optical measurement of a multi-stranded nucleic acid which comprises the step of bringing a compound into contact with a multi-stranded nucleic acid wherein said compound is capable of interacting with the multi-stranded nucleic acid, wherein the compound has the following properties: (a) the compound can exist in a substantially colorless and non-fluorescent state under at least one condition in an aqueous solution in the absence of the multi-stranded nucleic acid, and (b) when the multi-stranded nucleic acid is allowed to exist in the condition defined in the above (a), the compound changes to a substantially colored state based on an interaction with the multi-stranded nucleic acid and substantially expresses fluorescent property based on said interaction.

摘要翻译： 一种用于光学测量多链核酸的方法，其包括使化合物与多链核酸接触的步骤，其中所述化合物能够与多链核酸相互作用，其中该化合物具有以下 性质：（a）在不存在多链核酸的情况下，化合物可以在至少一种条件下在水溶液中以基本上无色和非荧光状态存在，和（b）当多链核酸为 允许以上述（a）中定义的条件存在，基于与多链核酸的相互作用，化合物变为基本上着色的状态，并且基于所述相互作用基本上表达荧光性质。

公开(公告)号：US07087744B2

公开(公告)日：2006-08-08

申请号：US10196223

申请日：2002-07-17

申请人： Junji Nishigaki ,  Kouki Nakamura ,  Kazuya Takeuchi ,  Hiroko Inomata ,  Masayoshi Kojima

发明人： Junji Nishigaki ,  Kouki Nakamura ,  Kazuya Takeuchi ,  Hiroko Inomata ,  Masayoshi Kojima

IPC分类号： C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q2563/107

摘要： Fluorescent nucleotides useful for labeling nucleic acids which are represented by the formula: X—Y—Z wherein X represents a residue of natural or non-natural nucleotide and the like and binds to Y at a basic moiety of the residue; Y represents a divalent bridging group or a single bond; Z represents a monovalent group derived from a compound represented by the formula (I) wherein R1 to R4 represent an alkyl group; V1 to V6 represent a hydrogen atom or a substituent; L1 to L7 represent a methine group; W represents an oxygen atom, a sulfur atom, —C(R3)(R4)— or —N(R5)— wherein R3 to R5 represents an alkyl group; Q represents a nitrogen atom or —C(V7)— wherein V7 represents a hydrogen atom or a monovalent substituent; M represents a counter ion; m represents a number required to neutralize the charge of the molecule; s, t and u represent 0 or 1 or the like, and binds to Y at a reactive group existing in R1 or R2

摘要翻译： 用于标记由式X-Y-Z表示的核酸的荧光核苷酸，其中X表示天然或非天然核苷酸的残基等，并与残基的碱性部分结合; Y表示二价桥基或单键; Z表示由式（I）表示的化合物衍生的单价基团，其中R 1至R 4代表烷基; V 1至V 6表示氢原子或取代基; L 1至L 7表示次甲基; W表示氧原子，硫原子，-C（R 3）（R 4） - 或-N（R 5） - 其中R 3至R 5表示烷基; Q表示氮原子或-C（V-7） - ，其中V 7表示氢原子或一价取代基; M表示抗衡离子; m表示中和分子电荷所需的数量; s，t和u表示0或1等，并且在R 1或R 2中存在的反应性基团与Y结合，

公开(公告)号：US20050130251A1

公开(公告)日：2005-06-16

申请号：US10485698

申请日：2002-08-02

申请人： Kazuaki Okabe ,  Akira Kadota ,  Kozo Aoki ,  Kazunobu Takahashi ,  Masami Sakurada ,  Kouki Nakamura

发明人： Kazuaki Okabe ,  Akira Kadota ,  Kozo Aoki ,  Kazunobu Takahashi ,  Masami Sakurada ,  Kouki Nakamura

IPC分类号： G01N31/22 ,  C12Q1/28

CPC分类号： G01N31/228 ,  G01N2333/908

摘要： A reagent for determination of hydrogen peroxide which comprises (A) a compound represented by the following general formula (I): R1—NH—R2   (I) (wherein R1 represents a carbamoyl group or the like, and R2 represents an arylamino group, a heteroarylamino group, or a substituent represented by the following general formula (II): [wherein R3 to R6 represent X—Y—Ra {Ra represents a hydrogen atom, an alkyl group or the like, X represents a single bond, O or the like, and Y represents a single bond, (C═O) or the like}, a cyano group, a halogen atom or the like]>; (B) a compound represented by the general formula (III): [wherein R9 represents a group which can be eliminated by oxidative coupling color-developing reaction with the compound represented by the formula (I), and R7, R8, and R10 to R13 have the same meaning as that of R3], or the like; and (C) a peroxidative substance.

摘要翻译： 一种用于测定过氧化氢的试剂，其包含（A）由以下通式（I）表示的化合物：<？in-line-formula description =“In-line formula”end =“lead”？> 1-N-R 2（I）<？在线公式描述=“在线公式”end =“tail”？>（其中R 1， / SUP>表示氨基甲酰基等，R 2表示芳基氨基，杂芳基氨基或由以下通式（II）表示的取代基：[其中R 3 至R 6表示XYR a R i表示氢原子，烷基等，X表示单 键，O等，Y表示单键，（CO）等}，氰基，卤素原子等〕;（B）由通式（III）表示的化合物：[ 其中R 9表示可以通过与式（I）表示的化合物的氧化偶联显色反应来除去的基团， R 7，R 8和R 10至R 13具有与R 10相同的含义， SUP> 3]等; 和（C）过氧化物质。

公开(公告)号：US06794509B1

公开(公告)日：2004-09-21

申请号：US10088232

申请日：2002-07-17

申请人： Junji Nishigaki ,  Kouki Nakamura

发明人： Junji Nishigaki ,  Kouki Nakamura

IPC分类号： C07D47102

CPC分类号： C07D471/04 ,  C07F9/6561

摘要： There are provided fluorescent compounds that can be used for DNA sequencing, measurement of physiologically active substance or the like based on fluorescence immunoassay and so forth. Compounds represented by the formula (I) (V1 to V6 represent a hydrogen atom or a functional group selected from the group consisting of a halogen atom, an alkyl group, an alkenyl group, a group that can form a covalent bond with a compound to be labeled and the like, and V1, V2 and others may bind to each other to form a saturated or unsaturated ring; R1 represents a hydrogen atom or a functional group selected from the group consisting of an alkyl group, an aryl group and a heterocyclic group; R3 and R4 represent an alkyl group, and R3 and R4 may bind to each other to form a ring; Q represents a group of atoms required to form a methine dye chromophore; and m and n represent 0 or 1, provided that m+n is 1) or salts thereof

摘要翻译： 提供了可用于DNA测序，基于荧光免疫测定等生理活性物质等的荧光化合物。由式（I）（V 1至V 6）表示的化合物代表氢 原子或选自卤素原子，烷基，烯基，可与待标记化合物形成共价键的基团等的官能团，V 1，V 2 并且其它可以彼此结合以形成饱和或不饱和环; R 1表示氢原子或选自烷基，芳基和杂环基的官能团; R 3， 和R 4表示烷基，R 3和R 4可以彼此结合形成环; Q表示形成次甲基染料发色团所需的原子团; m和n表示0 或1，条件是m + n为1）或其盐