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公开(公告)号：US5436159A
公开(公告)日：1995-07-25
申请号：US164294
申请日：1993-12-09
IPC分类号： C12N15/09 , C12N1/16 , C12N1/19 , C12N9/02 , C12N15/00 , C12R1/865 , C12N1/18 , C12N15/53 , C12N15/81
CPC分类号： C12Y106/02004 , C12N9/0042 , C12N9/0077 , C07K2319/00
摘要： The present invention provides a chimeric enzyme gene which codes for a monooxygenase having both monooxygenase activity derived from cytochrome P-450 and reducing power supplying ability derived from NADPH-cytochrome P-450 reductase.The present invention further provides a yeast expression plasmid which contains said chimeric enzyme gene and expresses said monooxygenase gene; a transformed yeast strain which carries said yeast expression plasmid; a monooxygenase which has both the monooxygenase activity and the reducing power supplying ability as mentioned above; and a process for producing said monooxygenase.
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2.发明授权Transformed yeasts having steroid-hydroxylase activity and process for hydroxylation of steroids using the said transformed yeasts 失效具有类固醇 - 羟化酶活性的转化的酵母和使用所述转化的酵母的类固醇羟基化的方法
公开(公告)号：US5137822A
公开(公告)日：1992-08-11
申请号：US232843
申请日：1988-08-16
CPC分类号： C12N9/0085 , C12Y114/99009 , Y10S435/942
摘要： Transformed yeasts with yeast expression plasmid containing yeast alcohol dehydrogenase promoter and bovine adrenocortical cytochrome P-450.sub.17.alpha. cDNA, which produce bovine adrenocortical cytochrome P-450.sub.17.alpha., and processes for producing 17-hydroxypregnenolone and 17-hydroxyprogesterone which comprises 17-hydroxylating pregnenolone or progesterone to 17-hydroxypregnenolone or 17-hydroxyprogesterone, respectively, with the said transformed yeasts.
摘要翻译： 用含有酵母醇脱氢酶启动子的酵母表达质粒和产生牛肾上腺皮质细胞色素P-45017α的牛肾上腺皮质细胞色素P-45017αcDNA的酵母表达质粒转化酵母，以及产生17-羟基孕烯醇酮和17-羟孕酮的方法，其包含17-羟化孕烯醇酮或孕酮 至17-羟基孕烯醇酮或17-羟孕酮分别与所述转化的酵母。
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3.发明授权Cytochrome P-450MC gene, expression plasmid carrying the said gene, yeasts transformed with the said plasmid and a process for producing cytochrome P-450MC by culturing the said transformant yeasts 失效细胞色素P-450MC基因，携带所述基因的表达质粒，用所述质粒转化的酵母和通过培养所述转化酵母产生细胞色素P-450MC的方法
公开(公告)号：US4766068A
公开(公告)日：1988-08-23
申请号：US741592
申请日：1985-06-05
申请人： Kenji Oeda , Toshiyuki Sakaki , Hideo Ohkawa , Yoshiyasu Yabusaki , Hiroko Murakami , Keiko Nakamura , Masatoshi Shimizu
发明人： Kenji Oeda , Toshiyuki Sakaki , Hideo Ohkawa , Yoshiyasu Yabusaki , Hiroko Murakami , Keiko Nakamura , Masatoshi Shimizu
CPC分类号： C12N9/0077 , C12N15/81
摘要： An expression vector, a plasmid containing yeast alcohol dehydrogenase I promoter and terminator and the gene coding for the rat liver cytochrome P-450MC gene Saccharomyces cerevisiae transformed with the plasmid and process for preparing rat liver cytochrome P-450MC.
摘要翻译： 表达载体，含有酵母醇脱氢酶I启动子和终止子的质粒和编码大鼠肝细胞色素P-450MC基因的基因用质粒转化的酿酒酵母（Saccharomyces cerevisiae），以及用于制备大鼠肝细胞色素P-450MC的方法。
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公开(公告)号：US5668000A
公开(公告)日：1997-09-16
申请号：US702795
申请日：1996-08-26
CPC分类号： C12N9/0077 , C12N15/625 , C12N15/81 , C12N9/0085 , C12P33/00 , C12P7/02 , C12Y114/99009 , C07K2319/00 , C07K2319/02 , C07K2319/07
摘要： The expression of mitochondrial P450 in yeast is disclosed. The mitochondrial P450 includes a chimeric P450 in which a signal sequence present at the N-terminus of a mammalian mitochondrial P450 has been substituted by a signal of a yeast mitochondrial protein and a chimeric P450 in which the latter signal has been further substituted by a targeting signal sequence to microsome.An expression plasmid for producing the enzyme in a large scale, a recombinant yeast strain carrying the expression plasmid, a process for producing the enzyme by the use of the recombinant yeast, and a process for producing 5.beta.-cholestane-3.alpha., 7.alpha., 12.alpha., 27-tetrol, 25-hydroxyvitamin D.sub.3 and 1.alpha.,25-dihydroxyvitamin D.sub.3 are also disclosed.
摘要翻译： 公开了线粒体P450在酵母中的表达。 线粒体P450包括嵌合P450，其中存在于哺乳动物线粒体P450的N末端的信号序列已被酵母线粒体蛋白的信号和嵌合P450取代，其中后一信号被靶向进一步取代 信号序列到微粒体。 用于大规模生产酶的表达质粒，携带表达质粒的重组酵母菌株，使用重组酵母生产酶的方法，以及生产5β-胆甾烷-3α，7α ，12α，27-四醇，25-羟基维生素D 3和1α，25-二羟基维生素D3。
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5.发明授权Method for safety evaluation of chemical compound using recombinant yeast expressing human cytochrome P450 失效使用表达人细胞色素P450的重组酵母对化合物进行安全性评估的方法公开(公告)号：US06620593B1
公开(公告)日：2003-09-16
申请号：US08277031
申请日：1994-07-19
申请人： Koji Hayashi , Toshiyuki Sakaki , Yoshiyasu Yabusaki , Koichiro Komai , Hideo Kaneko , Iwao Nakatsuka
发明人： Koji Hayashi , Toshiyuki Sakaki , Yoshiyasu Yabusaki , Koichiro Komai , Hideo Kaneko , Iwao Nakatsuka
IPC分类号： C12Q126
CPC分类号： C12Y106/02004 , C12N9/0038 , C12N9/0077 , C12Q1/025
摘要： There is disclosed a method for evaluation of the safety of a chemical compound, which includes the steps of: (a) reacting a chemical compound with recombinant yeast cells expressing, or in other words producing, human cytochrome P450 molecular species P450 1A2, P450 2C9, P450 2E1 and P450 3A4 together with a yeast NADPH-P450 reductase, which may be in the form of a fused enzyme with each of said human cytochrome P450 molecular species, or with the cell free extracts of the yeast cells; and (b) analyzing the resulting metabolite to determine the safety of the compound. According to this method, it can be determined whether a test compound will be converted into a carcinogenic or mutagenic form through the metabolism in the human liver, and whether the test compound or its metabolite has mutagenicity.
摘要翻译： 公开了一种用于评估化合物安全性的方法，其包括以下步骤：（a）使化学化合物与表达或换句话说，生成人细胞色素P450分子种类P450 1A2，P450 2C9的重组酵母细胞 ，P450 2E1和P450 3A4以及酵母NADPH-P450还原酶，其可以是与每种所述人细胞色素P450分子种类的融合酶的形式，或与酵母细胞的无细胞提取物; 和（b）分析得到的代谢物以确定化合物的安全性。 根据该方法，可以确定试验化合物是否将通过人肝脏中的代谢转化为致癌或诱变形式，以及试验化合物或其代谢物是否具有致突变性。
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6.发明申请公开(公告)号：US20130059341A1
公开(公告)日：2013-03-07
申请号：US13580316
申请日：2011-02-14
CPC分类号： C12N15/81 , C12N9/0006 , C12N9/1051 , C12P19/44
摘要： Provided is a new method for producing a glucuronide, having excellent productivity and being replaceable with a method using Saccharomyces pombe, and to provide a new means used in this production method. Disclosed are: a transformed Saccharomyces cerevisiae wherein a gene coding for a UDP-glucose dehydrogenase and a gene coding for a UDP-glucose transferase are inserted in a manner such that said genes can be expressed; a transformed Saccharomyces cerevisiae wherein a gene coding for a cytochrome P450 gene is further inserted in a manner such that said gene can be expressed; and a method for producing a glucaronide that includes culturing transformed Saccharomyces cerevisiae in the presence of glucose and a substance to be conjugated, generating the glucuronide of the aforementioned substance to be conjugated.
摘要翻译： 提供一种生产葡萄糖醛酸苷的新方法，具有优异的生产率并且可以用使用酿酒酵母的方法替代，并提供在该生产方法中使用的新方法。 公开了：转化的酿酒酵母，其中以可以表达所述基因的方式插入编码UDP-葡萄糖脱氢酶的基因和编码UDP-葡萄糖转移酶的基因; 转化的酿酒酵母，其中编码细胞色素P450基因的基因进一步以能够表达所述基因的方式插入; 以及一种生产葡糖多糖的方法，其包括在葡萄糖和待共轭物质的存在下培养转化的酿酒酵母，产生待共轭的上述物质的葡糖苷酸。
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7.发明授权Method for producing glucuronic acid conjugate using Saccharomyces cerevisiae 有权使用酿酒酵母生产葡萄糖醛酸结合物的方法公开(公告)号：US08741602B2
公开(公告)日：2014-06-03
申请号：US13580316
申请日：2011-02-14
IPC分类号： C12N15/81
CPC分类号： C12N15/81 , C12N9/0006 , C12N9/1051 , C12P19/44
摘要： Provided is a new method for producing a glucuronide, having excellent productivity and being replaceable with a method using Saccharomyces pombe, and to provide a new means used in this production method. Disclosed are: a transformed Saccharomyces cerevisiae wherein a gene coding for a UDP-glucose dehydrogenase and a gene coding for a UDP-glucose transferase are inserted in a manner such that said genes can be expressed; a transformed Saccharomyces cerevisiae wherein a gene coding for a cytochrome P450 gene is further inserted in a manner such that said gene can be expressed; and a method for producing a glucaronide that includes culturing transformed Saccharomyces cerevisiae in the presence of glucose and a substance to be conjugated, generating the glucuronide of the aforementioned substance to be conjugated.
摘要翻译： 提供一种生产葡萄糖醛酸苷的新方法，具有优异的生产率并且可以用使用酿酒酵母的方法替代，并提供在该生产方法中使用的新方法。 公开了：转化的酿酒酵母，其中以可以表达所述基因的方式插入编码UDP-葡萄糖脱氢酶的基因和编码UDP-葡萄糖转移酶的基因; 转化的酿酒酵母，其中编码细胞色素P450基因的基因进一步以能够表达所述基因的方式插入; 以及一种生产葡糖多糖的方法，其包括在葡萄糖和待共轭物质的存在下培养转化的酿酒酵母，产生待共轭的上述物质的葡糖苷酸。
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