公开(公告)号：US5436326A

公开(公告)日：1995-07-25

申请号：US208036

申请日：1994-03-09

申请人： Yoshizumi Ishino ,  Takashi Uemori ,  Kayo Fujita ,  Ikunoshin Kato

发明人： Yoshizumi Ishino ,  Takashi Uemori ,  Kayo Fujita ,  Ikunoshin Kato

IPC分类号： C12N9/12 ,  C12N15/54

CPC分类号： C12N9/1252 ,  C12Q2521/101

摘要： The present invention is directed to a method for cloning a gene for Pol I type DNA polymerase comprising;(a) amplifying target DNA with PCR using primers specific to said genes;(b) cloning a gene for Pol I type DNA polymerase with a probe selected from amplified DNA. And this invention is directed to a novel isolated gene coding for Pol I type DNA polymerase cloned in the plasmid.

摘要翻译： 本发明涉及用于克隆Pol I型DNA聚合酶的基因的方法，其包括： （a）使用特异于所述基因的引物用PCR扩增靶DNA; （b）用选自扩增的DNA的探针克隆Pol I型DNA聚合酶的基因。 本发明涉及编码克隆在质粒中的Pol I型DNA聚合酶的新型分离基因。

公开(公告)号：US5753482A

公开(公告)日：1998-05-19

申请号：US428823

申请日：1995-04-25

申请人： Yoshizumi Ishino ,  Takashi Uemori ,  Kayo Fujita ,  Ikunoshin Kato

发明人： Yoshizumi Ishino ,  Takashi Uemori ,  Kayo Fujita ,  Ikunoshin Kato

IPC分类号： C12N9/12 ,  C12N15/54

CPC分类号： C12N9/1252 ,  C12Q2521/101

摘要： The present invention is directed to a method for cloning a gene for Pol I type DNA polymerase comprising; (a) amplifying target DNA with PCR using primers specific to said genes; (b) cloning a gene for Pol I type DNA polymerase with a probe selected from amplified DNA. And this invention is directed to a novel isolated gene coding for Pol I type DNA polymerase cloned in the plasmid.

摘要翻译： 本发明涉及用于克隆Pol I型DNA聚合酶的基因的方法，其包括： （a）使用特异于所述基因的引物用PCR扩增靶DNA; （b）用选自扩增的DNA的探针克隆Pol I型DNA聚合酶的基因。 本发明涉及编码克隆在质粒中的Pol I型DNA聚合酶的新型分离基因。

公开(公告)号：US06395526B1

公开(公告)日：2002-05-28

申请号：US09091889

申请日：1998-06-26

申请人： Takashi Uemori ,  Yoshizumi Ishino ,  Ikunoshin Kato

发明人： Takashi Uemori ,  Yoshizumi Ishino ,  Ikunoshin Kato

IPC分类号： C12N912

CPC分类号： C12N9/1252

摘要： The present invention relates to a DNA polymerase possesses the properties of 1) exhibiting higher polymerase activity when assayed by using as a substrate a complex resulting from primer annealing to a single stranded template DNA, as compared to the case where an activated DNA is used as a substrate; 2) possessing a 3′→5′ exonuclease activity; 3) being capable of amplifying a DNA fragment of about 20 kbp, in the case where polymerase chain reaction (PCR) is carried out using &lgr;-DNA as a template. It also relates to a DNA polymerase-constituting protein; a DNA containing the base sequence encoding thereof; and a method for producing the DNA polymerase. The present invention provides a novel DNA polymerase possessing both a high primer extensibility and a 3′→5′ exonuclease activity.

摘要翻译： 本发明涉及DNA聚合酶，具有以下特征：1）与使用活化DNA作为单链模板DNA的情况相比，通过使用由单链模板DNA引物退火得到的复合物作为底物进行测定时，表现出更高的聚合酶活性 底物; 2）具有3'→5'核酸外切酶活性; 3）在使用羔羊DNA作为模板进行聚合酶链反应（PCR）的情况下能够扩增约20kbp的DNA片段。 它还涉及构建DNA聚合酶的蛋白质; 含有编码其碱基序列的DNA; 以及DNA聚合酶的制造方法。 本发明提供了具有高引物延伸性和3'-5'核酸外切酶活性的新型DNA聚合酶。

公开(公告)号：US09447388B2

公开(公告)日：2016-09-20

申请号：US14232174

申请日：2012-07-12

申请人： Yoshizumi Ishino ,  Takeshi Yamagami ,  Hiroaki Matsukawa ,  Takashi Uemori ,  Takehiro Sagara

发明人： Yoshizumi Ishino ,  Takeshi Yamagami ,  Hiroaki Matsukawa ,  Takashi Uemori ,  Takehiro Sagara

IPC分类号： C12N9/12 ,  C12N1/00 ,  C12N1/20 ,  C12N15/63 ,  C12P19/34

CPC分类号： C12N9/1252 ,  C12N1/00 ,  C12N1/20 ,  C12N15/63 ,  C12P19/34 ,  C12Y207/07007 ,  Y02P20/52

摘要： Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737,wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).

公开(公告)号：US20140322793A1

公开(公告)日：2014-10-30

申请号：US14232174

申请日：2012-07-12

申请人： Yoshizumi Ishino ,  Takeshi Yamagami ,  Hiroaki Matsukawa ,  Takashi Uemori ,  Takehiro Sagara

发明人： Yoshizumi Ishino ,  Takeshi Yamagami ,  Hiroaki Matsukawa ,  Takashi Uemori ,  Takehiro Sagara

IPC分类号： C12N9/12

CPC分类号： C12N9/1252 ,  C12N1/00 ,  C12N1/20 ,  C12N15/63 ,  C12P19/34 ,  C12Y207/07007 ,  Y02P20/52

摘要： Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737,wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).

摘要翻译： 提供各种新型DNA聚合酶。 本发明提供一种DNA聚合酶，其包含在SEQ ID NO：8的氨基酸序列之间插入9个氨基酸“-A737-A738-A739-A740-A741-A742-A743-A744-A745-”的氨基酸序列 位置736处的氨基酸残基和737位的氨基酸残基，其中：A737是具有非极性脂肪族侧链的氨基酸残基; A738是具有非极性脂肪族侧链的氨基酸残基; A739是具有带正电侧链的氨基酸残基; A740是具有带正电荷侧链的氨基酸残基; A741是具有非极性脂肪族侧链的氨基酸残基; A742是具有非极性脂肪族侧链的氨基酸残基; A743是任何给定的氨基酸残基; A744是具有带正电侧链的氨基酸残基; A745是具有非极性脂肪族侧链的氨基酸残基）。

公开(公告)号：US08344105B2

公开(公告)日：2013-01-01

申请号：US13239981

申请日：2011-09-22

申请人： Yoshimi Sato ,  Kazue Nishiwaki ,  Nana Shimada ,  Shigekazu Hokazono ,  Takashi Uemori ,  Hiroyuki Mukai ,  Ikunoshin Kato

发明人： Yoshimi Sato ,  Kazue Nishiwaki ,  Nana Shimada ,  Shigekazu Hokazono ,  Takashi Uemori ,  Hiroyuki Mukai ,  Ikunoshin Kato

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07K1/00

CPC分类号： C12Q1/686 ,  C12N9/1252 ,  C12Q2521/101

摘要： A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.

摘要翻译： 具有高保真DNA聚合酶活性的多肽，因此可用作遗传工程试剂; 编码该多肽的基因; 一种产生该多肽的方法; 以及通过使用多肽扩增核酸的方法。

公开(公告)号：US07790849B2

公开(公告)日：2010-09-07

申请号：US12241581

申请日：2008-09-30

申请人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimikazu Hashino ,  Ikunoshin Kato

发明人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimikazu Hashino ,  Ikunoshin Kato

IPC分类号： C07K14/00 ,  C07H21/04

CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/505 ,  C07K14/78 ,  C07K2319/00 ,  C12N9/1088 ,  C12N2740/13043 ,  C12N2740/13045

摘要： An isolated polypeptide includes the amino acid sequence shown in SEQ. ID No. 13 and an isolated nucleic acid encoding the polypeptide, such as an isolated nucleic acid including the nucleic acid sequence shown in SEQ. ID No. 17. The isolated polypeptide includes two heparin binding polypeptides connected in tandem and the isolated nucleic acid encodes these. The polypeptide and nucleic acid sequences can improve retroviral vector mediated gene transfer efficiency into target cells.

摘要翻译： 分离的多肽包括SEQ ID NO：1所示的氨基酸序列。 ID号13和编码该多肽的分离的核酸，例如分离的核酸，包括SEQ ID NO：1所示的核酸序列。 分离的多肽包括串联连接的两个肝素结合多肽，分离的核酸编码这些。 多肽和核酸序列可以改进逆转录病毒载体介导的靶细胞的基因转移效率。

公开(公告)号：US07572617B2

公开(公告)日：2009-08-11

申请号：US10526073

申请日：2003-08-26

申请人： Shigekazu Hokazono ,  Takashi Uemori ,  Tetsuki Tanaka ,  Ikunoshin Kato

发明人： Shigekazu Hokazono ,  Takashi Uemori ,  Tetsuki Tanaka ,  Ikunoshin Kato

IPC分类号： C07K14/00 ,  C12N9/16 ,  C12Q1/14 ,  C12N15/00 ,  C12N5/10 ,  C12N1/21 ,  C12P21/00 ,  C07H21/00

CPC分类号： C12N9/22

摘要： An isolated polypeptide having a thermostable ribonuclease H activity from Archaeoglobus profundus is highly useful in genetic engineering, as well as a gene encoding this polypeptide and a genetic engineering process for producing the polypeptide.

摘要翻译： 具有来自古细丝虫的热稳定核糖核酸酶H活性的分离的多肽在遗传工程中是非常有用的，以及编码该多肽的基因和用于产生多肽的基因工程方法。

公开(公告)号：US20060030046A1

公开(公告)日：2006-02-09

申请号：US11181091

申请日：2005-07-14

申请人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimikazu Hashino ,  Ikunoshin Kato

发明人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimikazu Hashino ,  Ikunoshin Kato

IPC分类号： C12N15/867 ,  C07K14/50

CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/505 ,  C07K14/78 ,  C07K2319/00 ,  C12N9/1088 ,  C12N2740/13043 ,  C12N2740/13045

摘要： A polypeptide represented by SEQ. ID No. 13, a polypeptide represented by SEQ. ID No. 30 or functional equivalents thereof and a polypeptide represented by SEQ. ID No. 17.

摘要翻译： SEQ ID NO： SEQ ID NO：13，SEQ ID NO： SEQ ID No 30或其功能等同物和SEQ ID NO： ID号17。

公开(公告)号：US5189144A

公开(公告)日：1993-02-23

申请号：US852343

申请日：1992-03-17

申请人： Kiyozo Asada ,  Takashi Uemori ,  Masahiro Endo ,  Fusao Kimizuka ,  Ikunoshin Kato

发明人： Kiyozo Asada ,  Takashi Uemori ,  Masahiro Endo ,  Fusao Kimizuka ,  Ikunoshin Kato

IPC分类号： C07K7/08 ,  C07K14/81

CPC分类号： C07K7/08 ,  C07K14/8139

摘要： Polypeptides are provided which have a calpain inhibitory activity. The polypeptides have an activity like that of natural human calpastatin but are significantly shorter in length than natural human calpastatin. The polypeptides are suited for synthetic manufacture.

摘要翻译： 提供具有钙蛋白酶抑制活性的多肽。 多肽具有类似于天然人钙蛋白酶抑制剂的活性，但其长度明显短于天然人钙蛋白酶抑制剂。 该多肽适用于合成制造。