利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

3 条记录 (耗时 0.035 秒)

    Method of isolating epithelial cells, method of preconditioning cells, and methods of preparing bioartificial skin and dermis with the epithelial cells or the preconditioned cells
    1.
    发明申请
    Method of isolating epithelial cells, method of preconditioning cells, and methods of preparing bioartificial skin and dermis with the epithelial cells or the preconditioned cells 审中-公开
    分离上皮细胞的方法，预处理细胞的方法以及用上皮细胞或预处理细胞制备生物人造皮肤和真皮的方法

    公开(公告)号：US20060105454A1

    公开(公告)日：2006-05-18

    申请号：US11312113

    申请日：2005-12-20

    申请人： Young-Sook Son ,  Hyun-Sook Park ,  Chun-Ho Kim ,  Hyun-Ju Kang ,  Chang-Hwan Kim ,  Youn-Young Kim ,  Su-Hyun Lee ,  Yong-Jae Gin

    发明人： Young-Sook Son ,  Hyun-Sook Park ,  Chun-Ho Kim ,  Hyun-Ju Kang ,  Chang-Hwan Kim ,  Youn-Young Kim ,  Su-Hyun Lee ,  Yong-Jae Gin

    IPC分类号： C12N5/06 ,  C12N5/08

    CPC分类号： C12N5/0698 ,  A61K35/12 ,  C12N5/0629 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2502/28 ,  C12N2509/00 ,  C12N2509/10

    摘要： A method of isolating epithelial cells from a human skin tissue or internal organ tissue using trypsin and ethylenediamine tetraacetic acid (EDTA) simultaneously with the application of magnetic stirring, a method of preconditioning isolated biological cells by the application of physical stimulus, i.e., strain, are provided. Epithelial cells can be isolated by the method with increased yield, colony forming efficiency (CFE), and colony size. Also, the increased percentage of stem cells in isolated cells is advantageous in therapeutic tissue implantation by autologous or allogeneic transplantation. In skin cells preconditioned by the application of strain, cell division is facilitated, and the secretion of extracellular matrix components and growth factors and the activity of matrix metalloproteinases (MMPs) are improved. When preconditioned cells are implanted by autologous or allogeneic transplantation to heal a damaged tissue, the improved cell adhesion, mobility, and viability provides a biological adjustment effect against a variety of stresses or physical stimuli which the cells would undergo after implantation, with improved capability of integration into host tissue, thereby markedly improving the probability of success in skin grafting.

    摘要翻译： 一种利用磁力搅拌同时使用胰蛋白酶和乙二胺四乙酸（EDTA）从人皮肤组织或内部器官组织分离上皮细胞的方法，一种通过应用物理刺激预处理分离的生物细胞的方法，即应变， 被提供。 可以通过增加产量，菌落形成效率（CFE）和菌落大小的方法分离上皮细胞。 此外，分离的细胞中干细胞的增加百分比在通过自体或同种异体移植治疗组织植入方面是有利的。 在通过应用菌株预处理的皮肤细胞中，促进细胞分裂，细胞外基质成分和生长因子的分泌以及基质金属蛋白酶（MMPs）的活性得到改善。 当通过自体或同种异体移植植入预处理细胞以愈合损伤的组织时，改善的细胞粘附性，迁移性和活力提供了针对细胞在植入后将经历的各种应力或物理刺激的生物调节作用，具有改善的 整合到宿主组织中，从而显着提高皮肤移植成功的可能性。

    Method of isolating epithelial cells, method of preconditioning cells, and methods of preparing bioartificial skin and dermis with the epithelial cells and preconditioned cells
    2.
    发明申请
    Method of isolating epithelial cells, method of preconditioning cells, and methods of preparing bioartificial skin and dermis with the epithelial cells and preconditioned cells 审中-公开
    分离上皮细胞的方法，预处理细胞的方法，以及用上皮细胞和预处理细胞制备生物人造皮肤和真皮的方法

    公开(公告)号：US20050164388A1

    公开(公告)日：2005-07-28

    申请号：US10258987

    申请日：2001-11-06

    申请人： Young-Sook Son ,  Hyun-Sook Park ,  Chun-Ho Kim ,  Hyun-Ju Kang ,  Chang-Hwan Kim ,  Youn-Young Kim ,  Young-Ju Choi ,  Su-Hyun Lee ,  Yong-Jae Gin

    发明人： Young-Sook Son ,  Hyun-Sook Park ,  Chun-Ho Kim ,  Hyun-Ju Kang ,  Chang-Hwan Kim ,  Youn-Young Kim ,  Young-Ju Choi ,  Su-Hyun Lee ,  Yong-Jae Gin

    IPC分类号： A61K35/12 ,  A61L27/00 ,  C12N5/071 ,  C12N5/08 ,  A61F2/10

    CPC分类号： C12N5/0698 ,  A61K35/12 ,  C12N5/0629 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2502/28 ,  C12N2509/00 ,  C12N2509/10

    摘要： A method of isolating epithelial cells from a human skin tissue or internal organ tissue using trypsin and ethylene-diamine tetraacetic acid (EDTA) simultaneously with the application of magnetic stirring, a method of preconditioning isolated biological cells by the application of physical stimulus, i.e., strain, are provided. Epithelial cells can be isolated by the method with increased yield, colony forming efficiency (CFE), and colony size. Also, the increased percentage of stem cells in isolated cells is advantageous in therapeutic tissue implantation by autologous or allogeneic transplantation. In skin cells preconditioned by the application of strain, cell division is facilitated, and the secretion of extracellular matrix components and growth factors and the activity of matrix metalloproteinases (MMPs) are improved. When preconditioned cells are implanted by autologous or allogeneic transplantation to heal a damaged tissue, the improved cell adhesion, mobility, and viability provides a biological adjustment effect against a variety of stresses or physical stimuli which the cells would undergo after implantation, with improved capability of integration into host tissue, thereby markedly improving the probability of success in skin grafting.

    摘要翻译： 一种利用磁力搅拌同时使用胰蛋白酶和乙二胺四乙酸（EDTA）从人皮肤组织或内脏器官组织分离上皮细胞的方法，一种通过应用物理刺激预处理分离的生物细胞的方法， 应变。 可以通过增加产量，菌落形成效率（CFE）和菌落大小的方法分离上皮细胞。 此外，分离的细胞中干细胞的增加百分比在通过自体或同种异体移植治疗组织植入方面是有利的。 在通过应用菌株预处理的皮肤细胞中，促进细胞分裂，细胞外基质成分和生长因子的分泌以及基质金属蛋白酶（MMPs）的活性得到改善。 当通过自体或同种异体移植植入预处理细胞以愈合损伤的组织时，改善的细胞粘附性，迁移性和活力提供了针对细胞在植入后将经历的各种应力或物理刺激的生物调节作用，具有改善的 整合到宿主组织中，从而显着提高皮肤移植成功的可能性。