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    Method of isolating epithelial cells, method of preconditioning cells, and methods of preparing bioartificial skin and dermis with the epithelial cells or the preconditioned cells
    1.
    发明申请
    Method of isolating epithelial cells, method of preconditioning cells, and methods of preparing bioartificial skin and dermis with the epithelial cells or the preconditioned cells 审中-公开
    分离上皮细胞的方法，预处理细胞的方法以及用上皮细胞或预处理细胞制备生物人造皮肤和真皮的方法

    公开(公告)号：US20060105454A1

    公开(公告)日：2006-05-18

    申请号：US11312113

    申请日：2005-12-20

    申请人： Young-Sook Son ,  Hyun-Sook Park ,  Chun-Ho Kim ,  Hyun-Ju Kang ,  Chang-Hwan Kim ,  Youn-Young Kim ,  Su-Hyun Lee ,  Yong-Jae Gin

    发明人： Young-Sook Son ,  Hyun-Sook Park ,  Chun-Ho Kim ,  Hyun-Ju Kang ,  Chang-Hwan Kim ,  Youn-Young Kim ,  Su-Hyun Lee ,  Yong-Jae Gin

    IPC分类号： C12N5/06 ,  C12N5/08

    CPC分类号： C12N5/0698 ,  A61K35/12 ,  C12N5/0629 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2502/28 ,  C12N2509/00 ,  C12N2509/10

    摘要： A method of isolating epithelial cells from a human skin tissue or internal organ tissue using trypsin and ethylenediamine tetraacetic acid (EDTA) simultaneously with the application of magnetic stirring, a method of preconditioning isolated biological cells by the application of physical stimulus, i.e., strain, are provided. Epithelial cells can be isolated by the method with increased yield, colony forming efficiency (CFE), and colony size. Also, the increased percentage of stem cells in isolated cells is advantageous in therapeutic tissue implantation by autologous or allogeneic transplantation. In skin cells preconditioned by the application of strain, cell division is facilitated, and the secretion of extracellular matrix components and growth factors and the activity of matrix metalloproteinases (MMPs) are improved. When preconditioned cells are implanted by autologous or allogeneic transplantation to heal a damaged tissue, the improved cell adhesion, mobility, and viability provides a biological adjustment effect against a variety of stresses or physical stimuli which the cells would undergo after implantation, with improved capability of integration into host tissue, thereby markedly improving the probability of success in skin grafting.

    摘要翻译： 一种利用磁力搅拌同时使用胰蛋白酶和乙二胺四乙酸（EDTA）从人皮肤组织或内部器官组织分离上皮细胞的方法，一种通过应用物理刺激预处理分离的生物细胞的方法，即应变， 被提供。 可以通过增加产量，菌落形成效率（CFE）和菌落大小的方法分离上皮细胞。 此外，分离的细胞中干细胞的增加百分比在通过自体或同种异体移植治疗组织植入方面是有利的。 在通过应用菌株预处理的皮肤细胞中，促进细胞分裂，细胞外基质成分和生长因子的分泌以及基质金属蛋白酶（MMPs）的活性得到改善。 当通过自体或同种异体移植植入预处理细胞以愈合损伤的组织时，改善的细胞粘附性，迁移性和活力提供了针对细胞在植入后将经历的各种应力或物理刺激的生物调节作用，具有改善的 整合到宿主组织中，从而显着提高皮肤移植成功的可能性。

    Method of isolating epithelial cells, method of preconditioning cells, and methods of preparing bioartificial skin and dermis with the epithelial cells and preconditioned cells
    2.
    发明申请
    Method of isolating epithelial cells, method of preconditioning cells, and methods of preparing bioartificial skin and dermis with the epithelial cells and preconditioned cells 审中-公开
    分离上皮细胞的方法，预处理细胞的方法，以及用上皮细胞和预处理细胞制备生物人造皮肤和真皮的方法

    公开(公告)号：US20050164388A1

    公开(公告)日：2005-07-28

    申请号：US10258987

    申请日：2001-11-06

    申请人： Young-Sook Son ,  Hyun-Sook Park ,  Chun-Ho Kim ,  Hyun-Ju Kang ,  Chang-Hwan Kim ,  Youn-Young Kim ,  Young-Ju Choi ,  Su-Hyun Lee ,  Yong-Jae Gin

    发明人： Young-Sook Son ,  Hyun-Sook Park ,  Chun-Ho Kim ,  Hyun-Ju Kang ,  Chang-Hwan Kim ,  Youn-Young Kim ,  Young-Ju Choi ,  Su-Hyun Lee ,  Yong-Jae Gin

    IPC分类号： A61K35/12 ,  A61L27/00 ,  C12N5/071 ,  C12N5/08 ,  A61F2/10

    CPC分类号： C12N5/0698 ,  A61K35/12 ,  C12N5/0629 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2502/28 ,  C12N2509/00 ,  C12N2509/10

    摘要： A method of isolating epithelial cells from a human skin tissue or internal organ tissue using trypsin and ethylene-diamine tetraacetic acid (EDTA) simultaneously with the application of magnetic stirring, a method of preconditioning isolated biological cells by the application of physical stimulus, i.e., strain, are provided. Epithelial cells can be isolated by the method with increased yield, colony forming efficiency (CFE), and colony size. Also, the increased percentage of stem cells in isolated cells is advantageous in therapeutic tissue implantation by autologous or allogeneic transplantation. In skin cells preconditioned by the application of strain, cell division is facilitated, and the secretion of extracellular matrix components and growth factors and the activity of matrix metalloproteinases (MMPs) are improved. When preconditioned cells are implanted by autologous or allogeneic transplantation to heal a damaged tissue, the improved cell adhesion, mobility, and viability provides a biological adjustment effect against a variety of stresses or physical stimuli which the cells would undergo after implantation, with improved capability of integration into host tissue, thereby markedly improving the probability of success in skin grafting.

    摘要翻译： 一种利用磁力搅拌同时使用胰蛋白酶和乙二胺四乙酸（EDTA）从人皮肤组织或内脏器官组织分离上皮细胞的方法，一种通过应用物理刺激预处理分离的生物细胞的方法， 应变。 可以通过增加产量，菌落形成效率（CFE）和菌落大小的方法分离上皮细胞。 此外，分离的细胞中干细胞的增加百分比在通过自体或同种异体移植治疗组织植入方面是有利的。 在通过应用菌株预处理的皮肤细胞中，促进细胞分裂，细胞外基质成分和生长因子的分泌以及基质金属蛋白酶（MMPs）的活性得到改善。 当通过自体或同种异体移植植入预处理细胞以愈合损伤的组织时，改善的细胞粘附性，迁移性和活力提供了针对细胞在植入后将经历的各种应力或物理刺激的生物调节作用，具有改善的 整合到宿主组织中，从而显着提高皮肤移植成功的可能性。

    HAND CARRY TYPE PORTABLE CURING APPARATUS USING LONG-ARC UV LAMP
    6.
    发明申请
    HAND CARRY TYPE PORTABLE CURING APPARATUS USING LONG-ARC UV LAMP 失效
    手持式便携式固化装置，使用长弧紫外线灯

    公开(公告)号：US20120161039A1

    公开(公告)日：2012-06-28

    申请号：US13031481

    申请日：2011-02-21

    申请人： Joo-Young YOON ,  Young-Il Youn ,  Chang-Hwan Kim ,  Ho-Keun Yoon

    发明人： Joo-Young YOON ,  Young-Il Youn ,  Chang-Hwan Kim ,  Ho-Keun Yoon

    IPC分类号： G21G1/12

    CPC分类号： B05D3/067

    摘要： A hand carry type portable curing apparatus using a long-arc UltraViolet (UV) lamp for concentrating the external air on the long-arc UV lamp and enhancing cooling efficiency by disposing a fan at a tilt angle and in addition installing a means for forcibly inducing a flow of air inside is provided. The apparatus includes a housing having an accepting part therein, the long-arc UV lamp installed in the accepting part of the housing, and a fan installed in the housing and cooling the long-arc UV lamp. The fan is installed on the slant to tilt toward the front in a front surface of the housing.

    摘要翻译： 一种携带式便携式固化装置，其使用长弧度紫外线（UV）灯，用于将外部空气浓缩在长弧形UV灯上，并且通过以倾斜角设置风扇来提高冷却效率，并且另外安装用于强制诱导的装置 提供内部的空气流。 该装置包括：壳体，其中具有接收部分，安装在壳体的接受部分中的长弧UV灯，以及安装在壳体中并且冷却长弧紫外灯的风扇。 风扇安装在斜面上，以朝向壳体前表面的前部倾斜。

    Systems and methods for touch-based two-stage text input
    10.
    发明授权
    Systems and methods for touch-based two-stage text input 有权
    基于触摸的两阶段文本输入的系统和方法

    公开(公告)号：US09256366B2

    公开(公告)日：2016-02-09

    申请号：US13584966

    申请日：2012-08-14

    申请人： Hui Dai ,  Chang-Ki Jeong ,  Chang-Hwan Kim ,  Jeong J. Ma ,  Phillip D. Rasky

    发明人： Hui Dai ,  Chang-Ki Jeong ,  Chang-Hwan Kim ,  Jeong J. Ma ,  Phillip D. Rasky

    IPC分类号： G06F3/041 ,  G06F3/0488

    CPC分类号： G06F3/04886

    摘要： Embodiments relate to systems and methods for touch-based two-stage text input. An electronic device (102), such as a wearable wireless device, can be configured with a two-stage input interface (116) on a display (104). A first stage can include a seek area (120), which displays a subset of letters or other symbols to represent the full range of symbolic elements that are available, in a compressed form. The user can identify an intended target range (128) by touching an area at or around the letter or other symbol of interest. That input triggers the display of a second stage of the interface in a separate selection area (122), in which all letters or other symbols in the target range are expanded and displayed. The user can then touch and lift off the letter or other symbol they wish to choose, for example, to insert in a message or application.

    摘要翻译： 实施例涉及用于基于触摸的两阶段文本输入的系统和方法。 诸如可穿戴式无线设备的电子设备（102）可以在显示器（104）上配置有两级输入接口（116）。 第一阶段可以包括搜索区域（120），其以压缩形式显示字母或其他符号的子集以表示可用的符号元素的全部范围。 用户可以通过触摸字母或其他感兴趣符号周围的区域来识别预期的目标范围（128）。 该输入触发在单独的选择区域（122）中显示界面的第二阶段，其中扩展和显示目标范围中的所有字母或其他符号。 然后，用户可以触摸并提起他们希望选择的字母或其他符号，例如插入消息或应用程序。