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    IN SITU AFFINITY MATURATION OF ANTIBODIES
    1.
    发明申请
    IN SITU AFFINITY MATURATION OF ANTIBODIES 审中-公开
    抗原的初步研究成果

    公开(公告)号：US20150377887A1

    公开(公告)日：2015-12-31

    申请号：US14769294

    申请日：2014-02-27

    Applicant: Yu-cheng SU ,  Steve R. ROFFLER ,  ACADEMIA SINICA

    Inventor： Yu-Cheng Su ,  Steve R. ROFFLER

    IPC: G01N33/577 ,  C07K16/40 ,  C07K16/44 ,  G01N33/68 ,  G01N33/50

    CPC classification number: G01N33/577 ,  C07K16/00 ,  C07K16/40 ,  C07K16/44 ,  C07K2317/14 ,  G01N33/5008 ,  G01N33/6854

    Abstract: This disclosure relates to a method to mimicking the germinal center reaction to generate antibodies with altered binding properties or increased affinity directly in hybridomas. To allow convenient and rapid affinity maturation of antibodies, a controllable activation-induced cytidine deaminase (AID) expression system to induce somatic hypermutation in hybridomas is developed. Selection of high affinity antibodies is achieved by fluorescence-activated cell sorting of hybridomas that preferentially bind fluorescence-labeled antigens. The disclosure also relates to de novo generation of hybridomas with tunable antibody affinity by generating myeloma fusion partners with controllable AID expression.

    Abstract translation: 本公开涉及模拟生发中心反应以产生具有改变的结合特性或直接在杂交瘤中增加的亲和力的抗体的方法。 为了允许抗体的方便和快速的亲和力成熟，开发了可控的活化诱导的胞苷脱氨酶（AID）表达系统以诱导杂交瘤中的体细胞超突变。 通过优先结合荧光标记的抗原的杂交瘤的荧光激活细胞分选来实现高亲和力抗体的选择。 本公开还涉及通过产生具有可控AID表达的骨髓瘤融合配偶体从而产生具有可调节抗体亲和力的杂交瘤。

    MEMBRANE BOUND REPORTER GENE SYSTEM
    7.
    发明申请
    MEMBRANE BOUND REPORTER GENE SYSTEM 审中-公开
    膜结合报告基因系统

    公开(公告)号：US20080176225A1

    公开(公告)日：2008-07-24

    申请号：US11624625

    申请日：2007-01-18

    Applicant: Steve ROFFLER ,  Tian-Lu Cheng ,  Yu-Cheng Su

    Inventor： Steve ROFFLER ,  Tian-Lu Cheng ,  Yu-Cheng Su

    IPC: C12Q1/68 ,  C07H21/04 ,  C12N15/55 ,  C12N15/85

    CPC classification number: C12Y302/01031 ,  A01K2227/105 ,  A01K2267/0393 ,  C12N9/2434 ,  C12N15/65 ,  C12N15/86 ,  C12N2740/13043 ,  C12Q1/6897

    Abstract: A recombinant DNA construct is provided and includes a first DNA fragment encoding a β-glucuronidase and a second DNA fragment encoding a membrane anchoring domain. The β-glucuronidase may be a human β-glucuronidase or a mouse β-glucuronidase. In one embodiment, an expression vector for delivering a gene of interest or portion thereof into a host cell includes a DNA sequence for the gene of interest, a first DNA fragment encoding a β-glucuronidase, and a second DNA fragment encoding a membrane anchoring domain. In another embodiment, a method of introducing a gene of interest or portion thereof into a host cell is provided, including introducing into the host cell a recombinant DNA construct.

    Abstract translation: 提供重组DNA构建体并且包括编码β-葡糖醛酸糖苷酶的第一DNA片段和编码膜锚定结构域的第二DNA片段。 β-葡糖苷酸酶可以是人β-葡糖醛酸糖苷酶或小鼠β-葡糖醛酸糖苷酶。 在一个实施方案中，用于将感兴趣的基因或其部分递送到宿主细胞中的表达载体包括目的基因的DNA序列，编码β-葡糖醛酸糖苷酶的第一DNA片段和编码膜锚定结构域的第二DNA片段 。 在另一个实施方案中，提供将感兴趣的基因或其部分引入宿主细胞的方法，包括向宿主细胞中引入重组DNA构建体。

    Pre-installation of pumping line for efficient fab expansion
    8.
    发明授权
    Pre-installation of pumping line for efficient fab expansion 失效

    公开(公告)号：US5718029A

    公开(公告)日：1998-02-17

    申请号：US746029

    申请日：1996-11-06

    Applicant: Cheng-Chang Hung ,  Horng-Jong Wang ,  Yu-Cheng Su

    Inventor： Cheng-Chang Hung ,  Horng-Jong Wang ,  Yu-Cheng Su

    IPC: H01L21/677 ,  B23Q17/00 ,  F16L5/00

    CPC classification number: H01L21/67727 ,  H01L21/67017 ,  Y10T137/6969 ,  Y10T29/49764 ,  Y10T29/4978

    Abstract: A procedure for the installation of clean room processing tools which require rough pumping equipment located in areas outside of the clean room. Instead of simultaneously installing and testing the pumping equipment and the processing tool, the pumping equipment is installed first along with its associated support services. Foreline piping, terminating in the proximity of the processing tool vacuum port is also installed and capped off at this time. Functional testing and qualification of the rough pumping equipment is done and then the processing tool is moved into place and connected to the pumping line on the clean room floor. This independent and sequential installation procedure reduces perturbations of on-going manufacturing activity in the clean room area and also minimizes the risk of costly damage to the processing tool by faulty, un-tested, pumping equipment. In addition, since the rough pumping equipment can be procured and installed before hand, time can be saved by getting the processing tool on-line faster.

    Pre-installation of pumping line for efficient fab expansion
    10.
    发明授权
    Pre-installation of pumping line for efficient fab expansion 失效
    泵送管线的预安装可以有效地进行厂房扩建

    公开(公告)号：US5867881A

    公开(公告)日：1999-02-09

    申请号：US949960

    申请日：1997-10-14

    Applicant: Cheng-Chang Hung ,  Horng-Jong Wang ,  Yu-Cheng Su

    Inventor： Cheng-Chang Hung ,  Horng-Jong Wang ,  Yu-Cheng Su

    IPC: H01L21/677 ,  B23Q17/00 ,  F16L5/00

    CPC classification number: H01L21/67727 ,  H01L21/67017 ,  Y10T137/6969 ,  Y10T29/49764 ,  Y10T29/4978

    Abstract: A procedure for the installation of clean room processing tools which require rough pumping equipment located in areas outside of the clean room. Instead of simultaneously installing and testing the pumping equipment and the processing tool, the pumping equipment is installed first along with its associated support services. Foreline piping, terminating in the proximity of the processing tool vacuum port is also installed and capped off at this time. Functional testing and qualification of the rough pumping equipment is done and then the processing tool is moved into place and connected to the pumping line on the clean room floor. This independent and sequential installation procedure reduces perturbations of on-going manufacturing activity in the clean room area and also minimizes the risk of costly damage to the processing tool by faulty, un-tested, pumping equipment. In addition, since the rough pumping equipment can be procured and installed before hand, time can be saved by getting the processing tool on-line faster.

    Abstract translation: 安装洁净室处理工具的程序，需要在洁净室外面的区域进行粗抽水设备。 泵送设备首先与其相关的支持服务一起安装，而不是同时安装和测试泵送设备和加工工具。 此外，在处理工具真空口附近终止的前级管路也安装并封盖。 完成粗抽设备的功能测试和鉴定，然后将处理工具移入到位，并连接到洁净室地板上的抽水管路。 这种独立且顺序的安装程序可以减少洁净室区域内正在进行的制造活动的干扰，并且还可以通过故障，未经测试的泵送设备降低对加工工具的昂贵损坏的风险。 另外，由于可以在手之前采购和安装粗抽设备，所以可以通过更快的在线加工工具节省时间。

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