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公开(公告)号：US20090325247A1
公开(公告)日：2009-12-31
申请号：US12096153
申请日：2006-12-01
申请人： Yukiko Kodama , Hideaki Nagano , Koichi Funato
发明人： Yukiko Kodama , Hideaki Nagano , Koichi Funato
IPC分类号： C12P7/64
CPC分类号： C12P13/02
摘要： The present invention provides methods for producing human ceramide in a yeast cell.The methods of the present invention comprise: 1) introducing the sphingoid Δ4-desaturase gene (DES1) by transformation of the yeast cell; and 2) abolishing the expression of the yeast sphinganine C4-hydroxylase gene (SUR2) by transformation of the yeast cell.
摘要翻译： 本发明提供了在酵母细胞中生产人神经酰胺的方法。 本发明的方法包括：1）通过转化酵母细胞引入鞘氨醇类脱氢酶脱氢酶基因（DES1）; 和2）通过酵母细胞的转化来消除酵母胞浆素C4-羟化酶基因（SUR2）的表达。
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公开(公告)号：US08367375B2
公开(公告)日：2013-02-05
申请号：US12663232
申请日：2008-05-20
申请人： Yukiko Kodama , Hiroaki Okuhara , Koichi Funato
发明人： Yukiko Kodama , Hiroaki Okuhara , Koichi Funato
摘要： The present invention provides a method for producing human ceramide in a yeast cell.The method of the present invention comprises: 1) introducing the sphingoid Δ4-desaturase gene (DES1) by transformation of the yeast cell; 2) abolishing the expression of the yeast sphinganine C4-hydroxylase gene (SUR2) by transformation of the yeast cell; and 3) abolishing the expression of the yeast sphingoid base kinase gene (LCB4) by transformation of the yeast cell.
摘要翻译： 本发明提供了在酵母细胞中生产人神经酰胺的方法。 本发明的方法包括：1）通过转化酵母细胞引入鞘氨醇和Dgr4-去饱和酶基因（DES1）; 2）通过转化酵母细胞消除酵母胞浆素C4-羟化酶基因（SUR2）的表达; 和3）通过转化酵母细胞来消除酵母鞘氨醇碱基激酶基因（LCB4）的表达。
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公开(公告)号：US20100304467A1
公开(公告)日：2010-12-02
申请号：US12663232
申请日：2008-05-20
申请人： Yukiko Kodama , Hiroaki Okuhara , Koichi Funato
发明人： Yukiko Kodama , Hiroaki Okuhara , Koichi Funato
IPC分类号： C12N1/15
摘要： The present invention provides a method for producing human ceramide in a yeast cell.The method of the present invention comprises: 1) introducing the sphingoid Δ4-desaturase gene (DES1) by transformation of the yeast cell; 2) abolishing the expression of the yeast sphinganine C4-hydroxylase gene (SUR2) by transformation of the yeast cell; and 3) abolishing the expression of the yeast sphingoid base kinase gene (LCB4) by transformation of the yeast cell.
摘要翻译： 本发明提供了在酵母细胞中生产人神经酰胺的方法。 本发明的方法包括：1）通过转化酵母细胞引入鞘氨醇和Dgr4-去饱和酶基因（DES1）; 2）通过转化酵母细胞消除酵母胞浆素C4-羟化酶基因（SUR2）的表达; 和3）通过转化酵母细胞来消除酵母鞘氨醇碱基激酶基因（LCB4）的表达。
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公开(公告)号：US20090297657A1
公开(公告)日：2009-12-03
申请号：US11887077
申请日：2006-08-21
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
CPC分类号： C12C12/006 , C12C12/004 , C12G1/0203 , C12N9/1085
摘要： The present invention relates to a brewery yeast having controlled hydrogen sulfide-producing capability, a process for producing alcoholic beverages with controlled hydrogen sulfide amount. More particularly, the present invention relates to a yeast whose hydrogen sulfide-producing capability that increases the product flavor is controlled by enhancing the expression level of MET17 gene encoding brewery yeast O-acetylhomoserinesulfhydorelace Met17p, particularly non-ScMET17 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.
摘要翻译： 本发明涉及一种具有受硫化氢生产能力的啤酒酵母，一种生产具有可控硫化氢含量的酒精饮料的方法。 更具体地说，本发明涉及通过提高编码啤酒酵母O-乙酰高丝氨酸亚磺基水合酶Met17p的MET17基因，特别是对大啤酒酵母特异性的非ScMET17基因的表达水平来控制增加产品风味的硫化氢生产能力的酵母， 以及用所述酵母生产酒精饮料的方法。
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公开(公告)号：US20090269437A1
公开(公告)日：2009-10-29
申请号：US11990930
申请日：2006-08-31
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12N1/19 , C12N15/11 , C07K14/00 , C12N15/00 , C12Q1/68 , C12C11/09 , C12G1/073 , A23L1/185 , A23L1/202
CPC分类号： C07K14/395 , C12C12/004 , C12C12/006 , C12G1/0203 , C12G3/02
摘要： The present invention relates to a glycerol channel gene and its uses, specifically, a brewery yeast producing alcoholic beverages with excellent body and mellowness, alcoholic beverages produced using the yeast, a process for producing the alcoholic beverages. More particularly, the present invention relates to a yeast whose capability of producing glycerol, which contribute to body and mellowness of products, was controlled by controlling expression level of FPS1 gene encoding brewery yeast glycerol channel Fps1p, particularly non-ScFPS1 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with the yeast.
摘要翻译： 本发明涉及一种甘油通道基因及其用途，具体地说，一种具有优异身体和醇厚度的啤酒酵母生产酒精饮料，使用酵母生产的含酒精饮料，生产酒精饮料的方法。 更具体地说，本发明涉及通过控制编码啤酒酵母甘油通道Fps1p的FPS1基因的表达水平，特别是对大啤酒特异性的非ScFPS1基因来控制有助于产物的身体和醇味的甘油的生产能力的酵母 酵母，以及用酵母生产酒精饮料的方法。
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公开(公告)号：US20090246316A1
公开(公告)日：2009-10-01
申请号：US11991065
申请日：2006-09-12
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12Q1/68 , C12N9/16 , C12N15/74 , C12N1/19 , G01N33/569 , C12C11/09 , C12G1/073 , C12Q1/44 , C12Q3/00 , C12R1/645
CPC分类号： C12N9/18 , C12C12/004 , C12C12/006 , C12G1/0203 , C12P7/62
摘要： The present invention relates to a esterase gene and its uses, specifically, a brewery yeast producing alcoholic beverages with excellent aroma and flavor, alcoholic beverages produced using the yeast, a process for producing the alcoholic beverages. More particularly, the present invention relates to a yeast whose capability of producing ester, which contribute to aroma and flavor of products, was controlled by regulating expression level of IAH1 gene encoding brewery yeast esterase Iah1p, particularly nonScIAH1 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with the yeast.
摘要翻译： 本发明涉及一种酯酶基因及其用途，具体地说，一种酿造具有优良芳香和风味的酒精饮料的啤酒酵母，使用该酵母生产的含酒精饮料，一种生产该酒精饮料的方法。 更具体地，本发明涉及通过调节编码啤酒酵母酯酶Iah1p的IAH1基因，特别是对啤酒酵母特异性的非scIAH1基因的表达水平来控制有助于产物的香味和风味的酵母的酵母，以及 涉及一种用酵母生产酒精饮料的方法。
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7.发明申请Gene capable of improving low temperature fermentability and/or low temperature resistance and use thererof 审中-公开能够改善低温发酵能力和/或耐低温性能的基因公开(公告)号：US20090175983A1
公开(公告)日：2009-07-09
申请号：US11990942
申请日：2006-08-31
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
CPC分类号： C07K14/395 , C12C11/003 , C12C12/004 , C12C12/006 , C12G1/0203 , C12G2200/11 , C12R1/85
摘要： The present invention relates to a gene capable of improving low temperature performance (low temperature fermentability and/or low temperature resistance) and use thereof, in particular, to a brewery yeast with superior low temperature performance, alcoholic beverages produced using said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose low temperature performance is improved by amplifying expression level of DLT1 gene encoding Dltlp capable of improving low temperature performance of a brewery yeast, especially non-ScDLT1 gene specific to a lager brewing yeast, and to a method for producing alcoholic beverages using said yeast.
摘要翻译： 本发明涉及能够改善低温性能（低温发酵性和/或耐低温性）及其使用，特别是具有优异的低温性能的啤酒酵母，使用所述酵母生产的含酒精饮料的基因和 生产所述饮料的方法。 更具体地说，本发明涉及通过扩增能够提高啤酒酵母的低温性能的特别是非特异性的不含ScDLT1基因的编码Dltlp的DLT1基因的表达水平来提高其低温性能的酵母，以及 涉及使用所述酵母生产酒精饮料的方法。
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8.发明申请公开(公告)号：US20090117227A1
公开(公告)日：2009-05-07
申请号：US12279376
申请日：2007-02-01
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
CPC分类号： C12Q1/42 , C12C12/004 , C12C12/006 , C12G1/0203 , C12N9/16 , C12Q1/02 , C12R1/85 , C12Y301/03012 , G01N2333/39
摘要： The present invention relates to a gene encoding trehalose-6-phosphate phosphatase and use thereof, in particular, a yeast for practical use with superior resistance property to dryness and/or low-temperature storage, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose resistance property to dryness and/or resistance property to low-temperature storage is enhanced by amplifying expression level of TPS2 gene encoding a trehalose-6-phosphate phosphatase Tps2p in brewer's yeast, especially non-ScTPS2 gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.
摘要翻译： 本发明涉及编码海藻糖-6-磷酸磷酸酶的基因及其用途，特别是用于实际使用的具有优异的耐干性和/或低温储存性的酵母，用所述酵母生产的含酒精饮料的方法和方法 用于生产所述饮料。 更具体地说，本发明涉及通过扩增酿酒酵母中编码海藻糖-6-磷酸磷酸酶Tps2p的TPS2基因的表达水平，特别是非抑制性酵母，其酵母对抗低温贮藏的耐干性和/ 特异于啤酒酿造酵母的-ScTPS2基因和用酵母等生产酒精饮料的方法
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公开(公告)号：US20090053359A1
公开(公告)日：2009-02-26
申请号：US12279080
申请日：2006-12-05
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12G1/00 , C07H21/00 , C07K14/00 , C12N15/00 , C12N15/63 , C12N1/19 , C12Q1/02 , C12Q1/68 , C12C11/00
CPC分类号： C07K14/435 , C12C12/004 , C12C12/006 , C12G1/0203
摘要： The present invention relates to ammonia transporter genes and use thereof. The invention relates in particular to a brewer's yeast which shows enhanced ammonia assimilation, alcoholic beverages produced using such yeast, and a method of producing such alcoholic beverages. More specifically, the invention relates to the MEP3 gene which codes for the ammonia transporter Mep3 in brewer's yeast, particularly to a yeast which can control the ammonia assimilation ability by controlling the level of expression of the nonScMEP3 gene or ScMEP3 gene characteristic to beer yeast and to a method of producing alcoholic beverages using such yeast.
摘要翻译： 本发明涉及氨转运蛋白基因及其应用。 本发明特别涉及显示增强的氨同化的酿酒酵母，使用这种酵母生产的含酒精饮料，以及生产这种酒精饮料的方法。 更具体地，本发明涉及编码啤酒酵母中氨转运蛋白Mep3的MEP3基因，特别是涉及通过控制啤酒酵母特有的nonScMEP3基因或ScMEP3基因的表达水平来控制氨同化能力的酵母， 涉及使用这种酵母生产酒精饮料的方法。
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10.发明授权Heat-resistant .beta.-galactosyltransferase, its production process and its use 失效耐热β-半乳糖转移酶，其生产工艺及其应用
公开(公告)号：US5861289A
公开(公告)日：1999-01-19
申请号：US55553
申请日：1993-05-03
申请人： Toru Nakayama , Yukiko Kodama , Norihide Amano , Masahiro Nakao , Yuji Shibano , Teruo Amachi
发明人： Toru Nakayama , Yukiko Kodama , Norihide Amano , Masahiro Nakao , Yuji Shibano , Teruo Amachi
CPC分类号： C12Y302/01023 , A23C9/1206 , C12N9/1051 , C12P19/18 , Y10S435/822
摘要： Described herein are a novel heat-resistant .beta.-galactosyltransferase, a production process of the enzyme and a utilization method of the enzyme. The enzyme is produced preferably by produced by a microorganism belonging to the family of Actinomycetaceae, which may be selected from fungi belonging to the genus Saccharopolyspora, the genus Thermomonospora or the genus Thermoactinomvces.
摘要翻译： 本文描述了新型耐热β-半乳糖基转移酶，酶的生产方法和酶的利用方法。 优选通过由属于放线菌科家族的微生物产生的酶，其可以选自属于Saccharopolyspora属，Thermomonospora属或Thermactinomvces属的真菌。
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