公开(公告)号：US5834644A

公开(公告)日：1998-11-10

申请号：US748635

申请日：1996-11-13

申请人： Zhifeng Shao ,  Jian Xun Mou ,  Gang Huang

发明人： Zhifeng Shao ,  Jian Xun Mou ,  Gang Huang

IPC分类号： G01Q60/00 ,  G01B11/30 ,  G01B21/30

CPC分类号： G01Q20/02 ,  B82Y35/00 ,  G01Q10/04 ,  G01Q60/38 ,  Y10S977/87

摘要： An atomic force microscope which can provide an automatic operation of focusing a laser beam onto a cantilever of the atomic force microscope, and which can ensure that a laser beam reflected off this cantilever is properly directed to a detector. A tip supported by the cantilever can also be automatically and properly lowered to a specimen. A piezotube scanner within such an atomic force microscope is also provided to prevent any damage from occurring if any leakage of an aqueous solution containing the specimen occurs.

摘要翻译： 一种原子力显微镜，可以提供将激光束聚焦到原子力显微镜的悬臂上的自动操作，并且可以确保从该悬臂反射的激光束被适当地引导到检测器。 由悬臂支撑的尖端也可以自动适当地降低到试样。 还提供这种原子力显微镜内的压电扫描仪，以防止发生含有样品的水溶液泄漏时的任何损坏。

公开(公告)号：US20070148690A1

公开(公告)日：2007-06-28

申请号：US11703921

申请日：2007-02-06

申请人： Zhifeng Shao ,  Sitong Sheng ,  Shoudan Liang

发明人： Zhifeng Shao ,  Sitong Sheng ,  Shoudan Liang

IPC分类号： C12Q1/68 ,  C12M3/00

CPC分类号： C12Q1/6876 ,  C12Q1/6809 ,  C12Q2600/158 ,  C12Q2565/519 ,  C12Q2537/155 ,  C12Q2537/149

摘要： This invention relates generally to analysis of gene expression profiles. In particular, the present invention provides a method for a method for analyzing gene expression profiles of a cell, which method comprises: a) providing for isolated mRNA or cDNA target sequences from a cell; b) sequentially hybridizing said isolated mRNA or cDNA target sequences with a plurality of nucleotide probes; and c) assessing the sequential hybridization between said isolated mRNA or cDNA target sequences and said plurality of nucleotide probes to analyze gene expression profiles of said cell. Systems for analyzing gene expression profiles are also provided. Optical devices for detecting hybridization signal are further provided.

摘要翻译： 本发明一般涉及基因表达谱的分析。 特别地，本发明提供了一种分析细胞基因表达谱的方法，该方法包括：a）从细胞提供分离的mRNA或cDNA靶序列; b）将所述分离的mRNA或cDNA靶序列与多个核苷酸探针顺序杂交; 和c）评估所述分离的mRNA或cDNA靶序列与所述多个核苷酸探针之间的顺序杂交，以分析所述细胞的基因表达谱。 还提供了分析基因表达谱的系统。 还提供用于检测杂交信号的光学装置。

公开(公告)号：US5079428A

公开(公告)日：1992-01-07

申请号：US401629

申请日：1989-08-31

申请人： Paul S. Da Lin ,  Zhifeng Shao

发明人： Paul S. Da Lin ,  Zhifeng Shao

IPC分类号： H01J37/141 ,  H01J37/244 ,  H01J37/28

CPC分类号： H01J37/141

摘要： A magnetic electron focusing lens including a first pole piece, from a back side of which low-energy electrons are injected, and a second pole piece located on a front side of the first pole piece. The first and second pole pieces are coupled to form a single magnetic circuit. The first pole piece is shaped to maximize the magnetic field at a its front side at a peak much closer to the first then to the second pole piece. The specimen is located between the first and second pole pieces near the peak of the magnetic field.

摘要翻译： 一种磁性电子聚焦透镜，包括从其背面注入低能电子的第一极片和位于第一极片前表面的第二极片。 第一和第二极片被耦合以形成单个磁路。 第一极片被成形为使其前侧处的磁场最大化，其峰值更接近第一极点到第二极靴。 样品位于靠近磁场峰值的第一和第二极片之间。

公开(公告)号：US08481266B2

公开(公告)日：2013-07-09

申请号：US12742995

申请日：2008-11-17

申请人： Zhifeng Shao ,  Sitong Sheng

发明人： Zhifeng Shao ,  Sitong Sheng

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6874 ,  C12Q2525/191 ,  C12Q2525/179 ,  C12Q2521/501 ,  C12Q2565/531

摘要： The present invention relates to the field of gene engineering, provides a DNA sequencing method and system. Said DNA sequencing method includes following steps: A. said DNA is processed into multiple DNA segments, and then constructed into multiple DNA tags; B. amplification of every single DNA tag, and then processed into single stranded DNA; C. Utilize the anchor which can ligate to DNA tags and possesses at least one degenerated base to sequence every single DNA tag and thus produce sequencing signal; D. Obtain sequences of every single DNA segment by sequencing signal. Said DNA sequencing system, includes: unit of short tags construction, unit of amplification, unit of sequencing reaction and unit of signal processing. In this invention, since DNA tags are sequenced by using sequencing anchor with at least one degenerative abase, length of DNA tags that can be directly sequenced is extended. Both short and long DNA tags can be sequenced. Thus application of DNA sequencing is expanded.

摘要翻译： 本发明涉及基因工程领域，提供了DNA测序方法和系统。 所述DNA测序方法包括以下步骤：A.将所述DNA加工成多个DNA片段，然后构建成多个DNA标签; B.扩增每个单个DNA标签，然后加工成单链DNA; C.利用可连接DNA标签的锚，并具有至少一个退化碱基来对每一个DNA标签进行排序，从而产生测序信号; D.通过测序信号获得每个DNA片段的序列。 所述DNA测序系统包括：短标签构建单位，放大单位，测序反应单位和信号处理单位。 在本发明中，由于通过使用具有至少一个退化性的测序锚来对DNA标签进行测序，所以扩展了可直接测序的DNA标签的长度。 短和长DNA标签都可以测序。 因此扩展了DNA测序的应用。

公开(公告)号：US20110045992A1

公开(公告)日：2011-02-24

申请号：US12742995

申请日：2008-11-17

申请人： Zhifeng Shao ,  Sitong Sheng

发明人： Zhifeng Shao ,  Sitong Sheng

IPC分类号： C40B30/00 ,  C12Q1/68 ,  C12M1/34 ,  C40B60/12

CPC分类号： C12Q1/6874 ,  C12Q2525/191 ,  C12Q2525/179 ,  C12Q2521/501 ,  C12Q2565/531

摘要： The present invention relates to the field of gene engineering, provides a DNA sequencing method and system. Said DNA sequencing method includes following steps: A. said DNA is processed into multiple DNA segments, and then constructed into multiple DNA tags; B. amplification of every single DNA tag, and then processed into single stranded DNA; C. Utilize the anchor which can ligate to DNA tags and possesses at least one degenerated base to sequence every single DNA tag and thus produce sequencing signal; D. Obtain sequences of every single DNA segment by sequencing signal. Said DNA sequencing system, includes: unit of short tags construction, unit of amplification, unit of sequencing reaction and unit of signal processing. In this invention, since DNA tags are sequenced by using sequencing anchor with at least one degenerative abase, length of DNA tags that can be directly sequenced is extended. Both short and long DNA tags can be sequenced. Thus application of DNA sequencing is expanded.

摘要翻译： 本发明涉及基因工程领域，提供了DNA测序方法和系统。 所述DNA测序方法包括以下步骤：A.将所述DNA加工成多个DNA片段，然后构建成多个DNA标签; B.扩增每个单个DNA标签，然后加工成单链DNA; C.利用可连接DNA标签的锚，并具有至少一个退化碱基来对每一个DNA标签进行排序，从而产生测序信号; D.通过测序信号获得每个DNA片段的序列。 所述DNA测序系统包括：短标签构建单位，放大单位，测序反应单位和信号处理单位。 在本发明中，由于通过使用具有至少一个退化性的测序锚来对DNA标签进行测序，所以扩展了可直接测序的DNA标签的长度。 短和长DNA标签都可以测序。 因此扩展了DNA测序的应用。

公开(公告)号：US07189509B2

公开(公告)日：2007-03-13

申请号：US10222459

申请日：2002-08-16

申请人： Zhifeng Shao ,  Sitong Sheng ,  Shoudan Liang

发明人： Zhifeng Shao ,  Sitong Sheng ,  Shoudan Liang

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6876 ,  C12Q1/6809 ,  C12Q2600/158 ,  C12Q2565/519 ,  C12Q2537/155 ,  C12Q2537/149

摘要： This invention relates generally to analysis of gene expression profiles. In particular, the present invention provides a method for a method for analyzing gene expression profiles of a cell, which method comprises: a) providing for isolated mRNA or cDNA target sequences from a cell; b) sequentially hybridizing said isolated mRNA or cDNA target sequences with a plurality of nucleotide probes; and c) assessing the sequential hybridization between said isolated mRNA or cDNA target sequences and said plurality of nucleotide probes to analyze gene expression profiles of said cell. Systems for analyzing gene expression profiles are also provided. Optical devices for detecting hybridization signal are further provided.

公开(公告)号：US06621079B1

公开(公告)日：2003-09-16

申请号：US09606343

申请日：2000-06-29

申请人： Zhifeng Shao ,  Gabor Szabo ,  Anders Mannelqvist

发明人： Zhifeng Shao ,  Gabor Szabo ,  Anders Mannelqvist

IPC分类号： G12K700

CPC分类号： G01Q60/44 ,  G01Q60/22

摘要： The present invention near field scanning optical microscope NSOM, and related method thereof, provides a high resolution image of a sample in aqueous solution without damaging the sample. This attribute will greatly expand the applications and utility of a NSOM in biomedicine, among other fields. Moreover, the NSOM can be further extended to include signals other than light. In operation, a pipette is filled with an electrolyte solution (aqueous solution) and lowered through the reservoir toward the surface of the sample while the current between the electrode inside the pipette and the electrode in the reservoir is monitored. As the tip of the pipette approaches the surface, the ion current decreases because the space through which ions can flow is reduced. The pipette is then scanned laterally over the surface and the path of the tip pipette follows the topography of the surface. An optical image is simultaneously provided as the light source is incident on the sample and the emitted light reaches the image acquisition device after being transmitted through the sample. The acquisition device converts the light energy into electrical signals where they are processed for displaying the acquired images on the monitor or printer.

摘要翻译： 本发明近场扫描光学显微镜NSOM及其相关方法提供了样品在水溶液中的高分辨率图像而不损伤样品。 这个属性将大大扩展NSOM在生物医药领域的应用和实用性。 此外，NSOM可以进一步扩展到包括除光之外的信号。 在操作中，移液管充满电解质溶液（水溶液），并通过储存器朝向样品表面降低，同时监测移液管内的电极与储液器中的电极之间的电流。 当吸管的尖端接近表面时，离子电流降低，因为离子可以流过的空间减小。 移液管然后在表面上横向扫描，并且尖端移液管的路径遵循表面的形貌。 当光源入射到样品上时，同时提供光学图像，并且发射的光在透射通过样品之后到达图像采集装置。 采集装置将光能转换成电信号，在那里它们被处理以在监视器或打印机上显示所获取的图像。