公开(公告)号：US20240360500A1

公开(公告)日：2024-10-31

申请号：US18535790

申请日：2023-12-11

申请人： Integrated DNA Technologies, Inc.

发明人： Vladimir MAKAROV ,  Julie LALIBERTE

IPC分类号： C12Q1/686 ,  C12N15/66 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6886

CPC分类号： C12Q1/686 ,  C12N15/66 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2521/501 ,  C12Q2521/525 ,  C12Q2521/531 ,  C12Q2525/186 ,  C12Q2525/191

摘要： Methods for performing multiplex PCR-based enrichment of a target substrate are provided. Systems and methods for generating a sequencing library are also provided.

公开(公告)号：US20240353327A1

公开(公告)日：2024-10-24

申请号：US18638714

申请日：2024-04-18

申请人： Leica Microsystems CMS GmbH ,  PixelBiotech GmbH ,  Integrated DNA Technologies, Inc.

发明人： Soeren ALSHEIMER ,  Yongsheng CHENG ,  Michael R. MARVIN

IPC分类号： G01N21/64

CPC分类号： G01N21/6428 ,  G01N2021/6441

摘要： A marker for analyzing a biological sample includes a label comprising an oligonucleotide backbone and at least a first detectable moiety, and an affinity reagent configured to specifically bind to a target molecule of the biological sample. the oligonucleotide backbone of the label is bound to the affinity reagent. The oligonucleotide backbone includes at least one cleavage site cleavable by a chemical agent.

公开(公告)号：US20240309350A1

公开(公告)日：2024-09-19

申请号：US18679324

申请日：2024-05-30

申请人： INTEGRATED DNA TECHNOLOGIES, INC.

发明人： Jessica Woodley ,  Bernice Thommandru ,  Joseph Dobosy ,  Mark Behlke ,  Adam Clore ,  Garrett Rettig ,  Beimeng Sun

IPC分类号： C12N9/22 ,  C12N15/113 ,  C12N15/66

CPC分类号： C12N9/22 ,  C12N15/113 ,  C12N15/66 ,  C12N2310/122 ,  C12N2310/20 ,  C12N2310/321 ,  C12N2310/531 ,  C12N2800/80

摘要： Described herein are compositions and methods for improving homology directed repair (HDR) efficiency and reducing homology-independent integration following introduction of double strand breaks with engineered nucleases. Additionally, modifications to double stranded DNA donors to improve the donor potency and efficiency of homology directed repair following introduction of double stranded breaks with programmable nucleases.

公开(公告)号：US11999994B2

公开(公告)日：2024-06-04

申请号：US17574842

申请日：2022-01-13

申请人： INTEGRATED DNA TECHNOLOGIES, INC.

发明人： Joseph Dobosy ,  Scott D. Rose ,  Jeffrey A. Manthey ,  Shawn D. Allen ,  Steven A. Henck ,  Mark Behlke

IPC分类号： C07H21/04 ,  C12N15/113 ,  C12Q1/6837 ,  C12Q1/6876

CPC分类号： C12Q1/6837 ,  C12N15/113 ,  C12Q1/6876 ,  C12N2310/11 ,  C12N2310/3519 ,  C12N2320/11

摘要： Described herein are compositions and methods for the production and quantification of barcoded or unique molecular identifier (UMI)-labeled substrates. In one aspect, the substrate is a bead comprising a template oligonucleotide that is elongated by successive extension reactions to provide a bead with an oligonucleotide comprising a plurality of barcodes and conserved anchor regions. Methods are also described for quantifying the amount of template oligonucleotide loaded onto the substrate and the products of the extension reaction after each round and after the final extension.

公开(公告)号：US20230133277A1

公开(公告)日：2023-05-04

申请号：US17870190

申请日：2022-07-21

申请人： INTEGRATED DNA TECHNOLOGIES, INC.

发明人： Christopher Anthony Vakulskas ,  Michael Allen Collingwood ,  Garrett Richard Rettig ,  Mark Aaron Behlke

IPC分类号： C12N15/11 ,  C12N9/22 ,  C12N15/90

摘要： This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRSPR/Cas endonuclease systems having mutant Cas9 proteins that display reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system.

公开(公告)号：US20230119227A1

公开(公告)日：2023-04-20

申请号：US17796350

申请日：2021-02-24

申请人： INTEGRATED DNA TECHNOLOGIES, INC.

发明人： An Ji ,  Michael Marvin ,  Kevin Marks ,  David Anderson

IPC分类号： C07H21/00 ,  C07F9/09 ,  C07H1/00

摘要： Functionalized solid supports are useful in the synthesis of oligonucleotides. The functionalized solid supports contain an extended linker to a terminal functional group or a first nucleotide or nucleoside moiety. The extended linker permits oligonucleotide synthesis to take place at a greater distance from the solid support with greater efficiency.

公开(公告)号：US20230049003A1

公开(公告)日：2023-02-16

申请号：US17235921

申请日：2021-04-20

申请人： Integrated DNA Technologies, Inc.

发明人： Michael Allen Collingwood ,  Steve Ehren Glenn ,  Christopher Anthony Vakulskas

IPC分类号： C12N9/22 ,  C07K14/47

摘要： This invention pertains to optimized protein fusion linkers for creating multi-functional chimeric proteins and methods of using the same. Additionally, the invention pertains to chimeric proteins for use in guided endonuclease systems.

公开(公告)号：US11242542B2

公开(公告)日：2022-02-08

申请号：US15964041

申请日：2018-04-26

申请人： Integrated DNA Technologies, Inc.

发明人： Christopher Anthony Vakulskas ,  Nicole Mary Bode ,  Michael Allen Collingwood ,  Garrett Richard Rettig ,  Mark Aaron Behlke

IPC分类号： C12N15/11 ,  C12N9/22 ,  C12N15/90 ,  C12N15/113 ,  C12N15/87 ,  C12N15/10 ,  C12Q1/6897 ,  C12N15/88 ,  C12P19/34

摘要： This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRISPR/Cas endonuclease systems having mutant Cas9 proteins that display reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system.

公开(公告)号：US10982247B2

公开(公告)日：2021-04-20

申请号：US15386631

申请日：2016-12-21

申请人： Integrated DNA Technologies, Inc.

发明人： Mark Aaron Behlke ,  Joseph A. Walder ,  Richard Owczarzy ,  Scott D. Rose ,  Joseph R. Dobosy ,  Susan M. Rupp

IPC分类号： C12P19/34 ,  C12N9/12

摘要： This invention relates to mutant DNA polymerases having an enhanced template discrimination activity compared with the corresponding unmodified DNA polymerase counterparts, wherein the amino acid sequence of the mutant DNA polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified Taq DNA polymerase.

公开(公告)号：US10767176B2

公开(公告)日：2020-09-08

申请号：US15299549

申请日：2016-10-21

申请人： INTEGRATED DNA TECHNOLOGIES, INC.

发明人： Michael Allen Collingwood ,  Ashley Mae Jacobi ,  Garrett Richard Rettig ,  Mollie Sue Schubert ,  Mark Aaron Behlke

IPC分类号： C12N15/11

摘要： This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRISPR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRISPR Cas9 endonuclease system.