公开(公告)号：US09234233B2

公开(公告)日：2016-01-12

申请号：US14578399

申请日：2014-12-20

申请人： Integrated DNA Technologies, Inc.

发明人： Scott Rose ,  Mark A. Behlke ,  Richard Owczarzy ,  Joseph A. Walder ,  Derek M. Thomas ,  Michael R. Marvin

IPC分类号： C07H21/02 ,  C07H19/04 ,  C12Q1/68 ,  C07H21/04 ,  G01N33/53 ,  G01N33/542

CPC分类号： C12Q1/6832 ,  C07H21/04 ,  C12Q1/6818 ,  C12Q1/6851 ,  G01N33/5308 ,  G01N33/542 ,  C12Q2525/185 ,  C12Q2525/197 ,  C12Q2565/101

摘要： A composition comprising an oligonucleotide having the structure 5′-Y1-L1-X-L2-Y2-3′. Y1 comprises a sequence of one or more DNA or RNA nucleotides, including a first nucleotide N1 having a 3′ phosphate covalently linked to L1. Y2 comprises a sequence of one or more DNA or RNA nucleotides, including a second nucleotide N2 having a 5′ phosphate covalently linked to L2. L1 and L2 each independently are a direct bond or a C1-C7 alkyl, alkynyl, alkenyl, heteroalkyl, substituted alkyl, aryl, heteroaryl, substituted aryl, cycloalkyl, alkylaryl, or alkoxyl group. X is R1 is a hydrogen or a C1-C8 alkyl. M is a label or ligand comprising a fused polycyclic aromatic moiety.

摘要翻译： 包含具有结构5'-Y1-L1-X-L2-Y2-3'的寡核苷酸的组合物。 Y1包含一个或多个DNA或RNA核苷酸的序列，包括与L1共价连接的3'磷酸的第一核苷酸N1。 Y2包含一个或多个DNA或RNA核苷酸的序列，包括与L2共价连接的5'磷酸二酯的第二核苷酸N2。 L 1和L 2各自独立地为直接键或C 1 -C 7烷基，炔基，烯基，杂烷基，取代的烷基，芳基，杂芳基，取代的芳基，环烷基，烷基芳基或烷氧基。 X是R 1是氢或C 1 -C 8烷基。 M是包含稠合多环芳族部分的标记或配体。

公开(公告)号：US20150191707A1

公开(公告)日：2015-07-09

申请号：US14542539

申请日：2014-11-14

申请人： Integrated DNA Technologies, Inc.

发明人： Mark Aaron Behlke ,  Joseph A. Walder ,  Richard Owczarzy ,  Scott D. Rose ,  Joseph R. Dobosy ,  Susan M. Rupp

IPC分类号： C12N9/12 ,  C12P19/34

CPC分类号： C12P19/34 ,  C12N9/1252 ,  C12Y207/07007

摘要： This invention relates to mutant DNA polymerases having an enhanced template discrimination activity compared with the corresponding unmodified DNA polymerase counterparts, wherein the amino acid sequence of the mutant DNA polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified Taq DNA polymerase.

摘要翻译： 本发明涉及与相应的未修饰的DNA聚合酶对应物相比具有增强的模板识别活性的突变DNA聚合酶，其中突变型DNA聚合酶的氨基酸序列在结构和功能上同源或直系同源位置783或784处包含至少一个取代 的未修饰的Taq DNA聚合酶。

公开(公告)号：US10982247B2

公开(公告)日：2021-04-20

申请号：US15386631

申请日：2016-12-21

申请人： Integrated DNA Technologies, Inc.

发明人： Mark Aaron Behlke ,  Joseph A. Walder ,  Richard Owczarzy ,  Scott D. Rose ,  Joseph R. Dobosy ,  Susan M. Rupp

IPC分类号： C12P19/34 ,  C12N9/12

摘要： This invention relates to mutant DNA polymerases having an enhanced template discrimination activity compared with the corresponding unmodified DNA polymerase counterparts, wherein the amino acid sequence of the mutant DNA polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified Taq DNA polymerase.

公开(公告)号：US20180044716A1

公开(公告)日：2018-02-15

申请号：US15672685

申请日：2017-08-09

申请人： Integrated DNA Technologies, Inc.

发明人： Joseph Dobosy ,  Sarah Jacobi ,  Richard Owczarzy ,  Mark Behlke

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12N9/22 ,  C12N15/52 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/6862 ,  C12Y301/26004 ,  C12Q2521/327 ,  C12Q2563/159

摘要： The invention is directed to methods and kits for performing an RNase H2-mediated cleavage reaction on a sample in an emulsion.

公开(公告)号：US20170260583A1

公开(公告)日：2017-09-14

申请号：US15604204

申请日：2017-05-24

申请人： Integrated DNA Technologies, Inc.

发明人： Joseph Dobosy ,  Richard Owczarzy ,  Mark Aaron Behlke

IPC分类号： C12Q1/68 ,  C12N15/11

CPC分类号： C12Q1/6876 ,  C07K2319/21 ,  C12N9/22 ,  C12N15/11 ,  C12N2310/20 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Y207/07007 ,  C12Y301/00 ,  C12Y301/26004 ,  C12Q2521/327 ,  C12Q2525/121 ,  C12Q2525/125 ,  C12Q2525/161 ,  C12Q2525/186

摘要： The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays. The invention also provides methods for detection of DNA sequences altered after cleavage by a targetable endonuclease, such as the CRISPR Cas9 protein from the bacterium Streptococcus pyogenes.

公开(公告)号：US20170204412A1

公开(公告)日：2017-07-20

申请号：US15360382

申请日：2016-11-23

申请人： Integrated DNA Technologies, Inc.

发明人： Mark Aaron Behlke ,  Richard Owczarzy ,  Yong You ,  Joseph Alan Walder ,  Kim Lennox

IPC分类号： C12N15/113 ,  C12Q1/68

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/141 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/344 ,  C12N2310/352 ,  C12N2310/3529 ,  C12N2320/51 ,  C12Q1/6876

摘要： The invention pertains to modifications for antisense oligonucleotides, wherein the modifications are used to improve stability and provide protection from nuclease degradation. The modifications could also be incorporated into double-stranded nucleic acids, such as synthetic siRNAs and miRNAs.

公开(公告)号：US09506059B2

公开(公告)日：2016-11-29

申请号：US14281646

申请日：2014-09-26

申请人： Integrated DNA Technologies, Inc.

发明人： Mark Aaron Behlke ,  Richard Owczarzy ,  Yong You ,  Joseph Alan Walder ,  Kim Lennox

IPC分类号： C12P19/34 ,  C12N15/113 ,  C12N15/11

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/141 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/344 ,  C12N2310/352 ,  C12N2310/3529 ,  C12N2320/51 ,  C12Q1/6876

摘要： The invention pertains to modifications for antisense oligonucleotides, wherein the modifications are used to improve stability and provide protection from nuclease degradation. The modifications could also be incorporated into double-stranded nucleic acids, such as synthetic siRNAs and miRNAs.

摘要翻译： 本发明涉及反义寡核苷酸的修饰，其中所述修饰用于改善稳定性并提供对核酸酶降解的保护。 这些修饰也可以并入双链核酸，如合成siRNA和miRNA。

公开(公告)号：US20150011744A1

公开(公告)日：2015-01-08

申请号：US14281646

申请日：2014-09-26

申请人： Integrated DNA Technologies, Inc.

发明人： Mark Aaron Behlke ,  Richard Owczarzy ,  Yong You ,  Joseph Alan Walder ,  Kim Lennox

IPC分类号： C12N15/113

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/141 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/344 ,  C12N2310/352 ,  C12N2310/3529 ,  C12N2320/51 ,  C12Q1/6876

摘要： The invention pertains to modifications for antisense oligonucleotides, wherein the modifications are used to improve stability and provide protection from nuclease degradation. The modifications could also be incorporated into double-stranded nucleic acids, such as synthetic siRNAs and miRNAs.

摘要翻译： 本发明涉及反义寡核苷酸的修饰，其中所述修饰用于改善稳定性并提供对核酸酶降解的保护。 这些修饰也可以并入双链核酸，如合成siRNA和miRNA。

公开(公告)号：US20220010346A1

公开(公告)日：2022-01-13

申请号：US17208286

申请日：2021-03-22

申请人： Integrated DNA Technologies, Inc.

发明人： Mark Aaron Behlke ,  Joseph A. Walder ,  Richard Owczarzy ,  Scott D. Rose ,  Joseph R. Dobosy ,  Susan M. Rupp

IPC分类号： C12P19/34 ,  C12N9/12

摘要： This invention relates to mutant Taq DNA polymerase having an enhanced template discrimination activity compared with an unmodified Taq DNA polymerase of SEQ ID NO.:1, wherein the amino acid sequence of the mutant Taq DNA polymerase consists of substitutions at residue positions 783, 784, or a combination of 783 and 784 of the unmodified Taq DNA polymerase of SEQ ID NO.:1.

公开(公告)号：US20210395799A1

公开(公告)日：2021-12-23

申请号：US17226044

申请日：2021-04-08

申请人： Integrated DNA Technologies, Inc.

发明人： Joseph Dobosy ,  Richard Owczarzy ,  Mark Aaron Behlke

IPC分类号： C12Q1/6827 ,  C12Q1/6858 ,  G16B30/00 ,  G16B20/20 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6853 ,  C12Q1/6876

摘要： The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays. The invention also provides methods for detection of DNA sequences altered after cleavage by a targetable endonuclease, such as the CRISPR Cas9 protein from the bacterium Streptococcus pyogenes.