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公开(公告)号：US07435723B2
公开(公告)日：2008-10-14
申请号：US10680742
申请日：2003-10-06
申请人： James E. Hagstrom , Mark Noble , Julia Hegge , Vladimir G. Budker
发明人： James E. Hagstrom , Mark Noble , Julia Hegge , Vladimir G. Budker
CPC分类号： C07H21/00 , A61K47/62 , A61K47/645 , A61K48/00 , C12N15/87
摘要： Disclosed is a system for providing in vivo delivery of polynucleotides to mammalian prostate cells using an intravascular administration route. The polynucleotides are inserted in an injection solution into a mammalian vasculature. Insertion of the injection solution at an appropriate rate increases the volume of extravascular fluid in the tissue thereby facilitating delivery of the polynucleotide to the cell.
摘要翻译： 公开了一种使用血管内施用途径向哺乳动物前列腺细胞体内递送多核苷酸的系统。 将多核苷酸插入到哺乳动物脉管系统的注射溶液中。 以适当的速率插入注射溶液会增加组织中血管外液体的体积，从而便于将多核苷酸递送至细胞。
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2.发明授权公开(公告)号：US07326780B2
公开(公告)日：2008-02-05
申请号：US11415021
申请日：2006-05-01
CPC分类号： C07D209/08 , C07D209/14 , C07H19/00 , C07H21/04
摘要： Compounds and methods are provided for a single-pot covalent attachment of a label to nucleic acids comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
摘要翻译： 提供了化合物和方法用于标记物与核酸的单罐共价连接，包括形成用于烷基化分子的共价连接的标记试剂。 然后，在标记试剂与分子具有反应性，从而形成共价键的条件下，将可共价连接的标记试剂与含有该分子的混合物组合。
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公开(公告)号：US07220400B2
公开(公告)日：2007-05-22
申请号：US10445696
申请日：2003-05-27
CPC分类号： A61K48/0041 , C12N15/87
摘要： Cyclic amidinium containing compounds and their methods of preparation are described. Compositions containing these compounds facilitate delivery of biologically active polymers to cells in vitro and in vivo.
摘要翻译： 描述了含有环脒的化合物及其制备方法。 含有这些化合物的组合物有助于将体内和体内的生物活性聚合物递送至细胞。
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公开(公告)号：US07071163B2
公开(公告)日：2006-07-04
申请号：US10633808
申请日：2003-08-04
申请人： Alexander V. Sokoloff , So Wong , Jon A. Wolff , Sean D. Monahan , James Ludtke , Lori Higgs , Darren Wakefield , Magdolna G. Sebestyén
发明人： Alexander V. Sokoloff , So Wong , Jon A. Wolff , Sean D. Monahan , James Ludtke , Lori Higgs , Darren Wakefield , Magdolna G. Sebestyén
IPC分类号： A61K38/00
CPC分类号： C07K14/005 , A61K41/00 , C12N2795/10222
摘要： We describe compounds that bind to and are internalized by hepatocytes. Association of these compounds to other molecules or complexes can be used to target the molecules or complexes to hepatocytes in vivo or in vitro.
摘要翻译： 我们描述与肝细胞结合并被内化的化合物。 这些化合物与其他分子或复合物的结合可用于在体内或体外靶向肝细胞的分子或复合物。
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公开(公告)号：US07396919B1
公开(公告)日：2008-07-08
申请号：US09328975
申请日：1999-06-09
申请人： Jon A. Wolff , Vladimir S. Trubetskoy , Aaron G. Loomis , Paul M. Slattum , Sean D. Monahan , James E. Hagstrom , Vladimir G. Budker
发明人： Jon A. Wolff , Vladimir S. Trubetskoy , Aaron G. Loomis , Paul M. Slattum , Sean D. Monahan , James E. Hagstrom , Vladimir G. Budker
摘要： An ionic polymer is utilized in “recharging” (another layer having a different charge) a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The resulting recharged complex can be formed with an appropriate amount of positive or negative charge such that the resulting complex has the desired net charge.
摘要翻译： 将离子聚合物用于“再充电”（具有不同电荷的另一层）缩合的多核苷酸复合物，用于向细胞递送核酸。 可以用合适量的正电荷或负电荷形成所得的再充电络合物，使得所得复合物具有所需的净电荷。
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6.发明授权公开(公告)号：US07208314B2
公开(公告)日：2007-04-24
申请号：US10083456
申请日：2002-02-26
IPC分类号： C12N5/00
CPC分类号： A61K48/0025 , A61K48/0008 , A61K48/0041 , A61K48/0075 , A61K48/0083
摘要： A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.
摘要翻译： 关于使用pH敏感递送系统将期望的化合物（例如药物和核酸）递送到细胞中的系统。 该系统提供用于将化合物递送和释放到细胞中的组合物和方法。
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公开(公告)号：US07192605B2
公开(公告)日：2007-03-20
申请号：US10161241
申请日：2002-05-31
申请人： Hans Herweijer , Vladimir G. Budker
发明人： Hans Herweijer , Vladimir G. Budker
IPC分类号： A61K9/127 , A61K31/7088 , C12N15/88
CPC分类号： C12N15/88 , A61K9/1272
摘要： The present invention relates to compositions and methods for transferring nucleic acids into cells in vitro and in vivo. The compositions comprise a transfection reagent and one or more detergents. In preferred embodiments, the compositions comprise delivery systems providing nucleic acid transfer complexes that transfect cells with high efficiency.
摘要翻译： 本发明涉及在体外和体内将核酸转运到细胞中的组合物和方法。 组合物包含转染试剂和一种或多种洗涤剂。 在优选实施方案中，组合物包含提供以高效转染细胞的核酸转移复合物的递送系统。
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公开(公告)号：US07144869B2
公开(公告)日：2006-12-05
申请号：US10310398
申请日：2002-12-05
CPC分类号： A61K48/0075
摘要： Processes are described for obtaining high levels of gene expression in primates after injection of nucleic acid to the liver via the lumen of the hepatic vein. The described process results in high level of gene expression with transient increases in liver enzymes.
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公开(公告)号：US07101995B2
公开(公告)日：2006-09-05
申请号：US10157674
申请日：2002-05-28
申请人： David L. Lewis , James E. Hagstrom , Hans Herweijer , Aaron G. Loomis , Sean D. Monahan , Jon A. Wolff
发明人： David L. Lewis , James E. Hagstrom , Hans Herweijer , Aaron G. Loomis , Sean D. Monahan , Jon A. Wolff
CPC分类号： C12N15/1137 , A61K31/58 , A61K31/74 , A61K38/00 , A61K48/00 , C12N15/111 , C12N15/88 , C12N2310/14 , C12N2320/32 , C12N2710/24111 , C12Y206/01002 , A61K2300/00
摘要： Described is a deliverable composition with low toxicity comprising an amphipathic compound, a polycation, and a siRNA. The composition may be used in the process of delivering a siRNA to an animal cell or more particularly, a mammal cell.
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公开(公告)号：US07098032B2
公开(公告)日：2006-08-29
申请号：US11046590
申请日：2005-01-28
申请人： Vladimir S. Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jon A. Wolff , David B. Rozema , Sean D. Monahan
发明人： Vladimir S. Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jon A. Wolff , David B. Rozema , Sean D. Monahan
IPC分类号： C12N15/88
CPC分类号： C12N15/88 , A61K47/60 , A61K47/645
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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