公开(公告)号：US20240309341A1

公开(公告)日：2024-09-19

申请号：US18660667

申请日：2024-05-10

申请人： Takara Bio Inc.

发明人： Takashi UEMORI ,  Hiroyuki Matsumoto ,  Kensuke Saito ,  Miwa Akitomo

IPC分类号： C12N9/12

CPC分类号： C12N9/1252 ,  C12Y207/07007

摘要： Provided are: a DNA polymerase mutant having reverse transcriptase activity, the DNA polymerase mutant including a sequence consisting of twelve specific amino acids A1-A12, wherein the DNA polymerase mutant having reverse transcriptase activity is characterized in that the A3 and/or A10 amino acid is substituted by a basic amino acid residue different from that prior to the introduction of mutation; a kit and a composition including the DNA polymerase; a method for producing the DNA polymerase; and a method for modifying an existing DNA polymerase having reverse transcriptase activity.

公开(公告)号：US20230330214A1

公开(公告)日：2023-10-19

申请号：US18028613

申请日：2021-09-30

申请人： OSAKA UNIVERSITY ,  TAKARA BIO INC. ,  ANGES, INC.

发明人： Hironori NAKAGAMI ,  Ryuichi MORISHITA ,  Hiroki HAYASHI ,  Yasunori AMAISHI ,  Sachiko OKAMOTO ,  Junichi MINENO ,  Hisato IKAI ,  Junko MICHIBATA ,  Takao KOMATSUNO

IPC分类号： A61K39/215 ,  A61P37/04

CPC分类号： A61K39/215 ,  A61P37/04 ,  A61K2039/53

摘要： Provided is DNA that: encodes a coronavirus (SARS CoV-2) spike protein or a fragment thereof; and has been optimized to partially or fully exhibit a codon included in a DNA sequence.

公开(公告)号：US20220372516A1

公开(公告)日：2022-11-24

申请号：US17767195

申请日：2020-10-09

申请人： TAKARA BIO INC.

发明人： Sachiko OKAMOTO ,  Izumi MAKI ,  Junichi MINENO

IPC分类号： C12N15/86 ,  C12N15/62 ,  C12N5/0783 ,  C12N15/113

摘要： Provided is a nucleic acid construct for expressing a gene of interest, the nucleic acid construct comprising, in order from 5′-terminus, each sequence of: (a) a 5′ long terminal repeat (LTR) sequence derived from a retrovirus; (b) a packaging signal sequence (IV) derived from a retrovirus; (c) a sequence or multiple cloning site of the gene of interest; (d) a post-transcriptional regulatory sequence (PRE); (e) an siRNA generating sequence which forms at least one stem-loop structure and in which RNA, which induces RNA interference in mammalian cells, is transcribed; and (f) a 3′ LTR sequence derived form a retrovirus.

公开(公告)号：US20210254018A1

公开(公告)日：2021-08-19

申请号：US16321112

申请日：2017-07-28

申请人： TAKARA BIO INC.

发明人： Tomomi OTSUJI ,  Toshikazu NISHIE ,  Risa KATO ,  Sachiko OKAMOTO ,  Tatsuji ENOKI ,  Junichi MINENO

IPC分类号： C12N5/074 ,  C12M1/12 ,  C07K14/78

摘要： Stem cells can be efficiently proliferated by culturing the stem cells in the presence of a novel recombinant fibronectin fragment.

公开(公告)号：US11046939B2

公开(公告)日：2021-06-29

申请号：US15779247

申请日：2016-11-24

申请人： KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION ,  TAKARA BIO INC.

发明人： Yoshizumi Ishino ,  Sonoko Ishino ,  Takeshi Yamagami ,  Takashi Uemori ,  Nariaki Takatsu

IPC分类号： C12N9/12 ,  C12N1/20 ,  C12N15/52 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12P19/34

摘要： The present invention relates to a Thermus aquaticus (Taq) polymerase having a strand displacement activity in which an amino acid residue in a template DNA binding site of the DNA polymerase is substituted with an amino acid to increase a total charge in the site, a nucleic acid encoding the polymerase, a vector containing the nucleic acid, a transformant containing the vector containing the nucleic acid or the nucleic acid, a method for producing the polymerase, a method for amplifying nucleic acids utilizing the polymerase, and a kit containing the polymerase. According to the present invention, a DNA polymerase having a high thermostability, capable of efficiently replicating a long-strand of a template DNA, and having a strong strand displacement activity is provided.

公开(公告)号：US20210164018A1

公开(公告)日：2021-06-03

申请号：US16616532

申请日：2018-06-06

申请人： TAKARA BIO INC.

发明人： Miwa AKITOMO ,  Takashi UEMORI

IPC分类号： C12Q1/6806 ,  C07K14/245

摘要： The present invention pertains to: an oligonucleotide preservation method; and a kit comprising an oligonucleotide. The present invention provides a method for stably preserving an oligonucleotide-containing solution by adding a nucleic acid-binding protein to said oligonucleotide-containing solution in advance.

公开(公告)号：US20210062141A1

公开(公告)日：2021-03-04

申请号：US16961773

申请日：2019-01-24

申请人： TAKARA BIO INC.

发明人： Tomomi OTSUJI ,  Yuka HIRASE ,  Asako HATSUYAMA ,  Sachiko OKAMOTO ,  Tatsuji ENOKI ,  Junichi MINENO

IPC分类号： C12N5/00 ,  C12N5/078 ,  C07K14/78

摘要： In the present invention, lymphocytes are efficiently grown by culturing lymphocytes in the presence of a novel recombinant fibronectin fragment.

公开(公告)号：US20200071675A1

公开(公告)日：2020-03-05

申请号：US16465920

申请日：2017-12-01

申请人： TAKARA BIO INC.

发明人： Yuki YAMAMOTO ,  Tatsuji ENOKI ,  Yasuhiro TOSAKA ,  Yoko YAMAGUCHI ,  Junichi MINENO

IPC分类号： C12N5/071 ,  A01N1/02

摘要： The present invention relates to a method for producing endothelial cells, including carrying out: (a) inducing a population of mesoderm-lineage cells containing endothelial progenitor cells from pluripotent stem cells without forming an embryoid body; and (b) culturing the population of mesoderm-lineage cells containing endothelial progenitor cells in the presence of RepSox, in this order. According to the present invention, endothelial cells with high quality can be efficiently produced from pluripotent stem cells. The endothelial cells obtained by the method of the present invention are useful for the production of, for example, a myocardial sheet, and expected to be utilized in the treatment of a heart disease. A myocardial sheet can be produced by mixing the endothelial cells obtained by the method of the present invention with myocardial cells and mural cells and culturing the cells.

公开(公告)号：US10336810B2

公开(公告)日：2019-07-02

申请号：US15550645

申请日：2016-02-11

申请人： University Health Network ,  Takara Bio Inc.

发明人： Shinya Tanaka ,  Naoto Hirano ,  Yuki Kagoya

IPC分类号： A61K35/00 ,  A61K38/00 ,  C07K16/28 ,  C12N15/62 ,  C07K14/705 ,  C07K14/715 ,  C07K14/725

摘要： Disclosed herein are chimeric antigen receptors (CARs) comprising an intracellular segment comprising an interleukin receptor chain, a JAK-binding motif, a Signal Transducer and Activator of Transcription (STAT) 5 association motif and/or a CD3ζ intracellular signaling domain comprising an exogenous STAT3 association motif, as well as cells and 5 compositions comprising said CARs and uses thereof.

公开(公告)号：US20190085307A1

公开(公告)日：2019-03-21

申请号：US16142643

申请日：2018-09-26

申请人： TAKARA BIO INC.

发明人： Kiyoyuki MATSUMURA ,  Nariaki TAKATSU ,  Takashi UEMORI ,  Hiroyuki MUKAI

IPC分类号： C12N9/22 ,  C12Q1/683 ,  C12P19/34

摘要： Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.