公开(公告)号：US20070009949A1

公开(公告)日：2007-01-11

申请号：US11515341

申请日：2006-09-01

申请人： Kaio Kitazato ,  Tsugumine Shu ,  Hidekazu Kuma ,  Yasuji Ueda ,  Makoto Asakawa ,  Mamoru Hasegawa ,  Akihiro Iida ,  Takahiro Hirata ,  Makoto Inoue ,  Yumiko Tokusumi

发明人： Kaio Kitazato ,  Tsugumine Shu ,  Hidekazu Kuma ,  Yasuji Ueda ,  Makoto Asakawa ,  Mamoru Hasegawa ,  Akihiro Iida ,  Takahiro Hirata ,  Makoto Inoue ,  Yumiko Tokusumi

IPC分类号： C12Q1/68 ,  C12N7/00 ,  C12N5/00 ,  C12N7/01 ,  C12N5/02

CPC分类号： C07K14/005 ,  A61K48/00 ,  A61K2039/5254 ,  A61K2039/5256 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/24143 ,  C12N2760/18811 ,  C12N2760/18822 ,  C12N2760/18823 ,  C12N2760/18843 ,  C12N2760/18845 ,  C12N2760/18861 ,  C12N2760/20222 ,  C12N2800/30 ,  C12N2810/6081

摘要： A functional RNP containing negative-strand single-stranded RNA derived from Sendai virus, which has been modified so as not to express at least one envelope protein, has been successfully prepared. An RNP comprising a foreign gene is prepared and inserted into a cell with the use of a cationic liposome, thereby successfully expressing the foreign gene.

摘要翻译： 已经成功地制备了已经被修饰以便不表达至少一种包膜蛋白的来源于仙台病毒的负链单链RNA的功能性RNP。 制备包含外来基因的RNP，并使用阳离子脂质体将其插入细胞中，从而成功地表达外源基因。

公开(公告)号：US20080207552A1

公开(公告)日：2008-08-28

申请号：US11982970

申请日：2007-11-05

申请人： Yoshiki Sawa ,  Ryuichi Morishita ,  Yasufumi Kaneda ,  Hikaru Matsuda ,  Toshiki Yoshimine

发明人： Yoshiki Sawa ,  Ryuichi Morishita ,  Yasufumi Kaneda ,  Hikaru Matsuda ,  Toshiki Yoshimine

IPC分类号： A61K31/7088 ,  A61P9/10

CPC分类号： A61K9/0019 ,  A61K9/127 ,  A61K31/711 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/4705 ,  C12N15/1131 ,  C12N15/115 ,  C12N15/86 ,  C12N2310/13 ,  C12N2310/315 ,  C12N2760/18811

摘要： The present invention provides introduction of NF-κB decoy oligodeoxynucleotide into rat cranial nerve through a carotid artery during global brain ischemia. Polymerase chain reaction demonstrated that one hour after global brain ischemia, transfected NF-κB decoy oligodeoxynucleotide effectively suppressed expression of tumor necrosis factor α, interleukin 1β and intracellular adhesion molecule 1 messenger RNAs. Terminal deoxynucleotidyl transferase-mediated deoxyuridine nick-end labeling staining and immunohistochemistry using microtubule-associated protein 2 demonstrated that transfected NF-κB decoy oligodeoxynucleotide significantly attenuated neuronal damage seven days after global brain ischemia. Therapeutic transfection of NF-κB decoy oligodeoxynucleotide during brain ischemia may be effective for attenuation of neuronal damage, suggesting a strategy for protecting the cerebrum from global ischemia.

摘要翻译： 本发明提供了在全脑缺血期间通过颈动脉将NF-κB诱饵寡脱氧核苷酸引入大鼠颅神经中。 聚合酶链反应表明，全球脑缺血后1小时，转染的NF-κB诱饵寡脱氧核苷酸有效抑制肿瘤坏死因子α，白细胞介素1β和细胞内粘附分子1信使RNA的表达。 末端脱氧核苷酸转移酶介导的脱氧尿苷切口末端标记染色和使用微管相关蛋白2的免疫组织化学证实，转染的NF-κB诱饵寡脱氧核苷酸在全脑缺血后7天显着减弱神经元损伤。 在脑缺血期间，NF-κB诱饵寡脱氧核苷酸的治疗转染对于减少神经元损伤可能是有效的，这表明保护大脑免受全身缺血的策略。

公开(公告)号：US20060135449A1

公开(公告)日：2006-06-22

申请号：US10509799

申请日：2002-03-29

申请人： Yoshiki Sawa ,  Ryuichi Morishita ,  Yasufumi Kaneda ,  Hikaru Matsuda ,  Toshiki Yoshimine

发明人： Yoshiki Sawa ,  Ryuichi Morishita ,  Yasufumi Kaneda ,  Hikaru Matsuda ,  Toshiki Yoshimine

IPC分类号： A61K48/00 ,  A61K9/127

CPC分类号： A61K9/0019 ,  A61K9/127 ,  A61K31/711 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/4705 ,  C12N15/1131 ,  C12N15/115 ,  C12N15/86 ,  C12N2310/13 ,  C12N2310/315 ,  C12N2760/18811

摘要： The present invention provides introduction of NF-κB decoy oligodeoxynucleotide into rat cranial nerve through a carotid artery during global brain ischemia. Polymerase chain reaction demonstrated that one hour after global brain ischemia, transfected NF-κB decoy oligodeoxynucleotide effectively suppressed expression of tumor necrosis factor α, interleukin 1β and intracellular adhesion molecule 1 messenger RNAs. Terminal deoxynucleotidyl transferase-mediated deoxyuridine nick-end labeling staining and immunohistochemistry using microtubule-associated protein 2 demonstrated that transfected NF-κB decoy oligodeoxynucleotide significantly attenuated neuronal damage seven days after global brain ischemia. Therapeutic transfection of NF-κB decoy oligodeoxynucleotide during brain ischemia may be effective for attenuation of neuronal damage, suggesting a strategy for protecting the cerebrum from global ischemia.

摘要翻译： 本发明提供了在全脑缺血期间通过颈动脉将NF-κB诱饵寡脱氧核苷酸引入大鼠颅神经中。 聚合酶链反应表明，全球脑缺血后1小时，转染的NF-κB诱饵寡脱氧核苷酸有效抑制肿瘤坏死因子α，白细胞介素1β和细胞内粘附分子1信使RNA的表达。 末端脱氧核苷酸转移酶介导的脱氧尿苷切口标记染色和使用微管相关蛋白2的免疫组织化学证实，转染的NF-κB诱饵寡脱氧核苷酸在全脑缺血后7天显着减弱神经元损伤。 在脑缺血期间，NF-κB诱饵寡脱氧核苷酸的治疗转染对于减少神经元损伤可能是有效的，这表明保护大脑免受全身缺血的策略。

公开(公告)号：US20030166252A1

公开(公告)日：2003-09-04

申请号：US10316538

申请日：2002-12-10

发明人： Kaio Kitazato ,  Tsugumine Shu ,  Hidekazu Kuma ,  Yasuji Ueda ,  Makoto Asakawa ,  Mamoru Hasegawa ,  Akihiro Iida ,  Takahiro Hirata ,  Makoto Inoue ,  Yumiko Tokusumi

IPC分类号： C12N015/86 ,  C12N007/00 ,  C12P021/06 ,  C12N007/01 ,  C12N015/00 ,  C12N015/09 ,  C12N015/63 ,  C12N015/70 ,  C12N015/74 ,  A61K039/155

CPC分类号： C07K14/005 ,  A61K48/00 ,  A61K2039/5254 ,  A61K2039/5256 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/24143 ,  C12N2760/18811 ,  C12N2760/18822 ,  C12N2760/18823 ,  C12N2760/18843 ,  C12N2760/18845 ,  C12N2760/18861 ,  C12N2760/20222 ,  C12N2800/30 ,  C12N2810/6081

摘要： A functional RNP containing negative-strand single-stranded RNA derived from Sendai virus, which has been modified so as not to express at least one envelope protein, has been successfully prepared. An RNP comprising a foreign gene is prepared and inserted into a cell with the use of a cationic liposome, thereby successfully expressing the foreign gene.

摘要翻译： 已经成功地制备了已经被修饰以便不表达至少一种包膜蛋白的来源于仙台病毒的负链单链RNA的功能性RNP。 制备包含外来基因的RNP，并使用阳离子脂质体将其插入细胞中，从而成功地表达外源基因。