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21 条记录 (耗时 0.018 秒)

    METHOD FOR PRODUCING DENDRITIC CELLS
    1.
    发明申请
    METHOD FOR PRODUCING DENDRITIC CELLS 有权
    生产细胞的方法

    公开(公告)号：US20120040458A1

    公开(公告)日：2012-02-16

    申请号：US13127753

    申请日：2009-11-13

    申请人： Yoshikazu Yonemitsu ,  Yasuji Ueda ,  Yui Harada

    发明人： Yoshikazu Yonemitsu ,  Yasuji Ueda ,  Yui Harada

    IPC分类号： C12N5/0784

    CPC分类号： C12N5/0639 ,  A61K39/00 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26

    摘要： An objective of the present invention is to provide methods for producing dendritic cells (DCs), which comprise the step of culturing DC precursor cells in the presence of a plurality of cytokines, produced dendritic cells, and uses thereof.The present inventors discovered that dendritic cells with a high IL-12 productivity can be obtained by culturing DC precursor cells in the presence of a plurality of cytokines, followed by about one week of culture in the presence of GM-CSF and IL-4. The present invention enables preparation of a large amount of DCs with a high IL-12 productivity from a small number of DC precursor cells, and therefore makes it easier to increase the number of DCs for administration in DC-based anti-tumor immune therapy, treatment of infections, etc. Thus, the effect of DC vaccines is expected to be enhanced.

    摘要翻译： 本发明的目的是提供用于制备树突状细胞（DC）的方法，其包括在多种细胞因子，产生的树突状细胞的存在下培养DC前体细胞的步骤及其应用。 本发明人发现通过在多种细胞因子的存在下培养DC前体细胞，然后在GM-CSF和IL-4存在下培养约1周，可获得具有高IL-12生产能力的树突状细胞。 本发明能够从少量的DC前体细胞制备大量具有高IL-12生产能力的DC，因此可以更容易地增加DC-抗肿瘤免疫治疗中用于给药的DC数量， 治疗感染等。因此，预期DC疫苗的效果将得到提高。

    VECTORS WITH MODIFIED PROTEASE-DEPENDENT TROPISM
    2.
    发明申请
    VECTORS WITH MODIFIED PROTEASE-DEPENDENT TROPISM 审中-公开
    具有修饰的蛋白依赖性TROPISM的载体

    公开(公告)号：US20100297732A1

    公开(公告)日：2010-11-25

    申请号：US12712905

    申请日：2010-02-25

    申请人： Hiroaki KINOH ,  Makoto Inoue ,  Yasuji Ueda ,  Akihiro Iida ,  Mamoru Hasegawa ,  Masanori Kobayashi

    发明人： Hiroaki KINOH ,  Makoto Inoue ,  Yasuji Ueda ,  Akihiro Iida ,  Mamoru Hasegawa ,  Masanori Kobayashi

    IPC分类号： C12N7/01 ,  C12N15/45

    CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/50 ,  C12N15/86 ,  C12N2760/18022 ,  C12N2760/18822 ,  C12N2760/18843 ,  C12N2800/30

    摘要： The present invention provides cell fusogenic vectors having replicative ability, whose protease-dependent tropism has been modified. M gene-deficient viral vectors encoding modified F proteins, in which the cleavage site of the F protein of paramyxovirus is modified to be cleaved by different proteases, were produced. In cells transfected with these vectors, the genomic RNA present in the vectors is replicated, and cell fusogenic infection spreads to neighboring cells depending on the presence of other proteases; however, no viral particles are released. The vectors of this invention, encoding the F proteins which are cleaved by proteases whose activity is enhanced in cancer, show cancer growth suppressive effect in vivo.

    摘要翻译： 本发明提供具有复制能力的细胞融合载体，其蛋白酶依赖性向性已被修饰。 产生编码修饰的F蛋白的M基因缺陷型病毒载体，其中修饰了被不同蛋白酶切割的副粘病毒的F蛋白的切割位点。 在用这些载体转染的细胞中，载体中存在的基因组RNA被复制，并且根据其它蛋白酶的存在，细胞融合感染扩散到相邻细胞; 然而，没有病毒颗粒被释放。 本发明的编码由蛋白酶切割的F蛋白的载体，其活性在癌症中增强，在体内显示出癌症生长抑制作用。

    Vectors with modified protease-dependent tropism
    3.
    发明授权
    Vectors with modified protease-dependent tropism 有权
    具有修饰的蛋白酶依赖性向量的载体

    公开(公告)号：US07709621B2

    公开(公告)日：2010-05-04

    申请号：US12140715

    申请日：2008-06-17

    申请人： Hiroaki Kinoh ,  Makoto Inoue ,  Yasuji Ueda ,  Akihiro Iida ,  Mamoru Hasegawa ,  Masanori Kobayashi

    发明人： Hiroaki Kinoh ,  Makoto Inoue ,  Yasuji Ueda ,  Akihiro Iida ,  Mamoru Hasegawa ,  Masanori Kobayashi

    IPC分类号： C07H21/04 ,  C12N15/45 ,  C12N7/00 ,  A61K48/00

    CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/50 ,  C12N15/86 ,  C12N2760/18022 ,  C12N2760/18822 ,  C12N2760/18843 ,  C12N2800/30

    摘要： The present invention provides cell fusogenic vectors having replicative ability, whose protease-dependent tropism has been modified. M gene-deficient viral vectors encoding modified F proteins, in which the cleavage site of the F protein of paramyxovirus is modified to be cleaved by different proteases, were produced. In cells transfected with these vectors, the genomic RNA present in the vectors is replicated, and cell fusogenic infection spreads to neighboring cells depending on the presence of other proteases; however, no viral particles are released. The vectors of this invention, encoding the F proteins which are cleaved by proteases whose activity is enhanced in cancer, show cancer growth suppressive effect in vivo.

    摘要翻译： 本发明提供具有复制能力的细胞融合载体，其蛋白酶依赖性向性已被修饰。 产生编码修饰的F蛋白的M基因缺陷型病毒载体，其中修饰了被不同蛋白酶切割的副粘病毒的F蛋白的切割位点。 在用这些载体转染的细胞中，载体中存在的基因组RNA被复制，并且根据其它蛋白酶的存在，细胞融合感染扩散到相邻细胞; 然而，没有病毒颗粒被释放。 本发明的编码由蛋白酶切割的F蛋白的载体，其活性在癌症中增强，在体内显示出癌症生长抑制作用。

    Process for producing virus vector containing membrane protein having sialic acid-binding in envelope with the use of gram-positive bacterium origin nueraminidase
    6.
    发明申请
    Process for producing virus vector containing membrane protein having sialic acid-binding in envelope with the use of gram-positive bacterium origin nueraminidase 审中-公开
    含有革兰氏阳性菌来源的神经氨酸酶的含有唾液酸结合的膜蛋白的病毒载体的方法

    公开(公告)号：US20060128019A1

    公开(公告)日：2006-06-15

    申请号：US10526650

    申请日：2003-09-04

    申请人： Masanori Kobayashi ,  Yasuji Ueda ,  Mamoru Hasegawa

    发明人： Masanori Kobayashi ,  Yasuji Ueda ,  Mamoru Hasegawa

    IPC分类号： C12N15/867 ,  C12N7/00

    CPC分类号： C12N15/86 ,  C12N2740/15043 ,  C12N2740/15045 ,  C12N2760/16122

    摘要： The present invention provides methods for producing a viral vector comprising a membrane protein that binds to sialic acid as a component of the envelope, using neuraminidase (NA) derived from Gram-positive bacteria. The methods comprise the steps of culturing cells producing a viral vector in the presence of an NA from Gram-positive bacteria, and recovering the produced virus. The methods of this invention enable the production of high titer virus at high cost performance. Such a viral vector is capable of transferring genes at high efficiency into cells such as blood cells and hematopoietic cells, including hematopoietic stem cells, and mucous cells including mucoepithelial cells, those not amenable to gene transfer by conventional methods, and therefore should be useful as a vector for gene therapy.

    摘要翻译： 本发明提供了使用衍生自革兰氏阳性细菌的神经氨酸酶（NA）产生包含结合唾液酸作为包膜的组分的膜蛋白的病毒载体的方法。 所述方法包括在来自革兰氏阳性菌的NA存在下培养产生病毒载体的细胞并回收所产生的病毒的步骤。 本发明的方法能够以高性价比的方式生产高滴度病毒。 这样的病毒载体能够将基因高效地转移到诸如血细胞和造血细胞（包括造血干细胞）和包括粘膜上皮细胞在内的粘液细胞（通过常规方法不适于基因转移的细胞）的细胞中，因此应该可用于 用于基因治疗的载体。