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公开(公告)号:US20140017726A1
公开(公告)日:2014-01-16
申请号:US13996345
申请日:2011-12-22
申请人: Yuansheng Yang , Mariati Mariati
发明人: Yuansheng Yang , Mariati Mariati
IPC分类号: C12N15/86
CPC分类号: C12N15/86 , A61K48/00 , C12N9/1077 , C12N15/67 , C12N15/85 , C12N2710/16122 , C12N2830/00 , C12N2830/46 , C12N2830/60 , C12N2830/85 , C12Y204/02007
摘要: The invention relates to a nucleic acid molecule comprising a functional promoter of a herpesvirus, a functional enhancer of a herpesvirus, and one or more internal elements of the CpG island of the aprt (adenine phosphoribosyl transferase) gene and/or a functional variant thereof. A method of producing a desired polypeptide using the nucleic acid molecule, a vector and a host cell containing the nucleic acid molecule are also disclosed.
摘要翻译: 本发明涉及包含疱疹病毒的功能性启动子,疱疹病毒的功能性增强子和aprt(腺嘌呤磷酸核糖基转移酶)基因的CpG岛的一个或多个内部元件和/或其功能变体的核酸分子。 还公开了使用核酸分子,载体和含有核酸分子的宿主细胞产生所需多肽的方法。
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公开(公告)号:US20100151572A1
公开(公告)日:2010-06-17
申请号:US11914563
申请日:2006-05-16
CPC分类号: A61K38/45 , A61K48/005 , C12N9/1077 , C12N9/1258 , C12Y204/02007 , Y02A50/473
摘要: Described are methods and compositions for inhibiting undesired cells by expression of one or more exogenous enzymes in the cells and administration of a prodrug which is a substrate for at least one of the enzymes to produce a cytotoxic compound. Inventive methods and compositions are active to inhibit cells expressing the exogenous enzymes as well as bystander cells. Tumor cells are a particular target for inhibition using methods and compositions detailed according to the present invention. Provided are methods and compositions for improved anti-tumoral effects by overexpression of adenine phosphoribosyltransferase (APRT) to produce cytotoxins which inhibit the cells overexpressing the APRT as well as bystander cells. Overexpression of APRT in conjunction with expression of E. coli PNP and administration of a substrate for E. coli PNP provides particularly effective anti-tumoral effects.
摘要翻译: 描述了通过在细胞中表达一种或多种外源酶来抑制不需要的细胞的方法和组合物,并且给予作为至少一种酶产生细胞毒性化合物的底物的前药。 本发明的方法和组合物是有活性的以抑制表达外源酶的细胞以及旁观者细胞。 使用根据本发明详细描述的方法和组合物,肿瘤细胞是抑制的特定靶标。 提供了用于通过腺嘌呤磷酸核糖转移酶(APRT)过表达改善抗肿瘤作用以产生抑制过表达APRT的细胞以及旁观者细胞的细胞毒素的方法和组合物。 APRT的表达与大肠杆菌PNP的表达和大肠杆菌PNP的底物的表达相结合提供特别有效的抗肿瘤效果。
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公开(公告)号:US20050181388A1
公开(公告)日:2005-08-18
申请号:US10958216
申请日:2004-10-04
申请人: Aled Edwards , Akil Dharamsi , Masoud Vedadi , Muhammad Alam , Cheryl Arrowsmith , Donald Awrey , Bryan Beattie , Kristina Buzadzija , Veronica Canadien , Megan Domagala , Simon Houston , Dhushy Kanagarajah , Qin Li , Kamran Mansoury , Merry-Lynn McDonald , Kathleen Nethery-Brokx , Ivy Ng , Hui Ouyang , Benjamin Pinder , Dawn Richards , Matthew Tai , Rosanne Thalakada , Francois Vallee , Cristina Virag
发明人: Aled Edwards , Akil Dharamsi , Masoud Vedadi , Muhammad Alam , Cheryl Arrowsmith , Donald Awrey , Bryan Beattie , Kristina Buzadzija , Veronica Canadien , Megan Domagala , Simon Houston , Dhushy Kanagarajah , Qin Li , Kamran Mansoury , Merry-Lynn McDonald , Kathleen Nethery-Brokx , Ivy Ng , Hui Ouyang , Benjamin Pinder , Dawn Richards , Matthew Tai , Rosanne Thalakada , Francois Vallee , Cristina Virag
IPC分类号: C07K14/245 , C07K14/315 , C07K14/32 , C12N9/00 , C12N9/02 , C12N9/04 , C12N9/10 , C12N9/12 , C12N9/14 , C12N9/16 , C12N9/78 , C12N9/90 , C12Q1/68 , G01N33/00
CPC分类号: C12N9/0008 , C07K14/245 , C07K14/3156 , C07K14/32 , C07K2319/00 , C12N9/0006 , C12N9/1007 , C12N9/1077 , C12N9/1085 , C12N9/1205 , C12N9/1229 , C12N9/1235 , C12N9/1241 , C12N9/14 , C12N9/16 , C12N9/78 , C12N9/90 , C12N9/93 , C12Y101/01158 , C12Y102/01011 , C12Y201/01033 , C12Y201/01045 , C12Y201/01061 , C12Y204/02007 , C12Y205/01007 , C12Y207/01026 , C12Y207/01048 , C12Y207/04008 , C12Y207/06001 , C12Y207/07003 , C12Y207/07023 , C12Y207/07038 , C12Y304/11018 , C12Y304/24057 , C12Y305/04005 , C12Y306/01001 , C12Y501/03001 , C12Y504/02003 , C12Y599/01003 , C12Y601/01014 , C12Y601/0102 , C12Y601/01021 , C12Y603/02004 , C12Y603/02008 , C12Y603/02013 , C12Y603/04014 , C12Y604/01002
摘要: The present invention relates to polypeptide targets for pathogenic bacteria. The invention also provides biochemical and biophysical characteristics of those polypeptides.
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公开(公告)号:US20060078977A1
公开(公告)日:2006-04-13
申请号:US11167900
申请日:2005-06-27
申请人: Aled Edwards , Akil Dharamsi , Masoud Vedadi , Teresa Clarke
发明人: Aled Edwards , Akil Dharamsi , Masoud Vedadi , Teresa Clarke
CPC分类号: C12N9/1007 , C07K14/315 , C07K2299/00 , C12N9/1077 , C12N9/1229 , C12N9/1235 , C12N9/1241 , C12N9/14 , C12N9/78 , C12Y201/01045 , C12Y204/02007 , C12Y306/01023 , Y02A90/26
摘要: The present invention relates to novel drug targets for pathogenic bacteria. Accordingly, the invention provides purified protein comprising the amino acid sequence set forth in SEQ ID NO: 4. The invention also provides biochemical and biophysical characteristics of the polypeptides of the invention.
摘要翻译: 本发明涉及病原菌的新型药物靶标。 因此,本发明提供了包含SEQ ID NO:4所示氨基酸序列的纯化蛋白质。本发明还提供本发明多肽的生物化学和生物物理学特征。
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