公开(公告)号：US12060596B2

公开(公告)日：2024-08-13

申请号：US16399145

申请日：2019-04-30

申请人： INV NYLON CHEMICALS AMERICAS, LLC

发明人： Alexander Brett Foster ,  Cristina Serrano Amatriain ,  Gary J. Smith ,  Paul Sheldon Pearlman ,  Mark Paul Taylor ,  Jonathan Combe ,  Daniel Bawdon

IPC分类号： C12P7/625 ,  C12N1/20 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12P7/04 ,  C12P7/06 ,  C12P7/42

CPC分类号： C12P7/625 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/88 ,  C12P7/04 ,  C12P7/065 ,  C12P7/42 ,  C12Y101/01001 ,  C12Y203/01016 ,  C12Y208/03015 ,  C12Y401/01004 ,  C12N2500/02 ,  C12N2500/05 ,  C12N2500/12 ,  C12N2500/24

摘要： Provided herein are methods for increasing the yield of an extracellular product synthesized by an organism cultured in a continuous aerobic fermentation system. The extracellular product yield is increased through the use of an organism modified to decreased production of polyhydroxyalkanoate, to increase production of the extracellular product, and to include promoters that can be inducible in response to nutrient limitation conditions. The extracellular product yield is also increased by operating the continuous fermentation system under particular nutrient limitation conditions. Also provided are non-naturally occurring organisms that have been modified for use with the provided methods, and extracellular products made using the provided methods.

公开(公告)号：US11702680B2

公开(公告)日：2023-07-18

申请号：US16399155

申请日：2019-04-30

申请人： INV Nylon Chemicals Americas, LLC

发明人： Alexander Brett Foster ,  Cristina Serrano Amatriain ,  Gary J. Smith ,  Paul Sheldon Pearlman ,  Mark Paul Taylor

IPC分类号： C12P7/625 ,  C12N1/20 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12P7/04 ,  C12P7/06 ,  C12P7/42

CPC分类号： C12P7/625 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/88 ,  C12P7/04 ,  C12P7/065 ,  C12P7/42 ,  C12Y101/01001 ,  C12Y203/01016 ,  C12Y208/03015 ,  C12Y401/01004 ,  C12N2500/02 ,  C12N2500/05 ,  C12N2500/12 ,  C12N2500/24

摘要： Provided herein are methods for generating cellular biomass in continuous aerobic fermentation systems. The biomass yield, and the concentration of polyhydroxyalkanoate within the biomass, are each directed to advantageous levels by operating the continuous fermentation system under particular nutrient limitation conditions. Also provided are biomass produced using the provided methods, and animal feed compositions including the provided biomass.