公开(公告)号：US20170328922A1

公开(公告)日：2017-11-16

申请号：US15528166

申请日：2015-03-19

申请人： UNIVERSITY OF SOUTH AUSTRALIA ,  CURTIN UNIVERSITY OF TECHNOLOGY

发明人： Doug Brooks ,  Sally Plush ,  Massimiliano Massi

IPC分类号： G01N33/92 ,  C07D471/04 ,  G01N33/50 ,  G01N33/58 ,  C07D257/04 ,  G01N33/574 ,  C07F13/00

CPC分类号： G01N33/92 ,  C07D257/04 ,  C07D401/04 ,  C07D471/04 ,  C07F13/00 ,  G01N33/5005 ,  G01N33/574 ,  G01N33/586 ,  G01N2405/02 ,  G01N2405/06 ,  G01N2405/10

摘要： The present disclosure relates to methods and products for labelling, binding and/or detection of lipids. Certain embodiments provide a method of labelling a lipid. The method comprises exposing the lipid to a complex comprising a transition metal carbonyl compound, a conjugated bidentate ligand and a tetrazolato compound, and thereby labelling the lipid by binding the complex to the lipid.

公开(公告)号：US20140329787A1

公开(公告)日：2014-11-06

申请号：US14352611

申请日：2012-10-17

申请人： Deakin University

发明人： Dean Ian Phillips ,  Peter Luke Beech

IPC分类号： C12N9/16 ,  G01N33/92 ,  C12P9/00 ,  C12Q1/44 ,  A01N45/00 ,  C12P7/64

CPC分类号： C12N9/16 ,  A01N37/46 ,  A01N45/00 ,  A01N61/00 ,  C07K2319/02 ,  C07K2319/21 ,  C10L1/026 ,  C12P7/6418 ,  C12P7/6436 ,  C12P7/6445 ,  C12P7/649 ,  C12P9/00 ,  C12Q1/44 ,  C12Y301/04003 ,  G01N33/92 ,  G01N2333/916 ,  G01N2405/06 ,  G01N2500/02 ,  G01N2500/04 ,  Y02E50/13

摘要： The present invention relates to a novel Phytophthora phospholipase C and uses thereof, methods of identifying modulators and inhibitors of a biological function of the phospholipase C, and methods of inhibiting Phytophthora growth comprising inhibiting a biological function of a novel Phytophthora phospholipase C.

摘要翻译： 本发明涉及一种新型疫霉属磷脂酶C及其用途，鉴定调节剂和磷脂酶C生物学功能抑制剂的方法，以及抑制疫霉属生长的方法，包括抑制新型疫霉属磷脂酶C的生物学功能。

公开(公告)号：US20120225447A1

公开(公告)日：2012-09-06

申请号：US13508381

申请日：2010-11-05

申请人： Wonhwa Cho

发明人： Wonhwa Cho

IPC分类号： C12Q1/02 ,  C12N9/96 ,  C07K14/00

CPC分类号： G01N33/56966 ,  G01N33/582 ,  G01N33/92 ,  G01N2405/04 ,  G01N2405/06 ,  G01N2800/044

摘要： Provided herein are fluorescent lipid binding proteins (FLBPs). The FLBPs comprise a lipid binding domain linked to a fluorophore, whrereby the fluorophore's fluorescence emission undergoes a spectral change upon lipid binding. Said fluorophore is selected from the group consisting of 2-dimethylamino-6-acyl-naphthalene (DAN) and RED fluorophore and said lipid binding protein is selected from the group consisting of ENTH domain of epsin 1, C2 domain of bovine lactadherin, C1B domain of protein kinase C-gamma, C2 domain of cytosolic phospholipase A2-beta, and PH domain of Bruton's tyrosine kinase PH.

摘要翻译： 本文提供荧光脂质结合蛋白（FLBPs）。 FLBPs包含与荧光团连接的脂质结合结构域，因为荧光团的荧光发射在脂质结合时经历光谱变化。 所述荧光团选自2-二甲基氨基-6-酰基 - 萘（DAN）和RED荧光团，所述脂质结合蛋白选自由ε1的ENTH结构域，牛乳内链素的C2结构域，C1B结构域 蛋白激酶C-γ，胞质磷脂酶A2-β的C2结构域和Bruton酪氨酸激酶PH的PH结构域。

公开(公告)号：US07629130B2

公开(公告)日：2009-12-08

申请号：US11525334

申请日：2006-09-23

申请人： Hubert Hilbi

发明人： Hubert Hilbi

IPC分类号： C12Q1/00 ,  G01N33/53 ,  G01N33/563 ,  G01N33/543 ,  G01N33/531 ,  G01N33/533

CPC分类号： C07K14/195 ,  A61K38/00 ,  C07K2319/20 ,  C07K2319/40 ,  G01N33/92 ,  G01N2333/195 ,  G01N2405/06 ,  Y02A50/478

摘要： Methods for detecting and quantifying phosphoinositide lipids in a sample are provided, together with novel SidC- and SdcA-derived polypeptide fragments, or fusion proteins comprising such fragments that may be effectively employed as PI(4)P probes in biochemical and cell biological assays. Applications of the disclosed probes include: (i) detection and quantification of PI(4)P in vitro; and (ii) staining of intracellular compartments in live or fixed eukaryotic cells.

摘要翻译： 提供了用于检测和定量样品中的磷酸肌醇脂质的方法以及新型的SidC和SdcA衍生的多肽片段，或包含这样的片段的融合蛋白，其可以在生物化学和细胞生物学测定中有效地用作PI（4）P探针。 所公开的探针的应用包括：（i）体外PI（4）P的检测和定量; 和（ii）在活的或固定的真核细胞中染色细胞内区室。

公开(公告)号：US20050003358A1

公开(公告)日：2005-01-06

申请号：US10485667

申请日：2002-08-05

申请人： Christopher Parish ,  Vivian Cabalda-Crane

发明人： Christopher Parish ,  Vivian Cabalda-Crane

IPC分类号： G01N27/62 ,  B01J20/283 ,  C07K7/08 ,  C07K14/47 ,  C12Q1/68 ,  G01N27/64 ,  G01N30/26 ,  G01N30/72 ,  G01N30/88 ,  G01N33/483 ,  G01N33/574 ,  G01N33/68

CPC分类号： G01N33/6848 ,  G01N30/7233 ,  G01N33/574 ,  G01N33/57484 ,  G01N33/57488 ,  G01N33/6851 ,  G01N2405/06

摘要： Provided are novel cancer markers for the diagnosis of cancer in humans and non-human mammalian subjects, specifically a cancer marker comprising a negatively-charged molecule with a mass/charge (m/z) ratio of about 991. The cancer marker of the invention may be used to determine the presence of one or more cancerous cells or tumors in a biological sample by assaying the sample for a reduced level of said cancer marker.

摘要翻译： 本发明提供了用于人和非人哺乳动物受试者中癌症诊断的新型癌症标志物，特别是包含质量/电荷（m / z）比为约991的带负电荷的分子的癌症标志物。本发明的癌症标志物 可用于通过测定样品以降低水平的所述癌症标志物来确定生物样品中一种或多种癌细胞或肿瘤的存在。

公开(公告)号：US06306598B1

公开(公告)日：2001-10-23

申请号：US09337973

申请日：1999-06-21

申请人： Deborah H. Charych ,  Ulrich Jonas

发明人： Deborah H. Charych ,  Ulrich Jonas

IPC分类号： C12Q168

CPC分类号： B82Y30/00 ,  A61K9/1273 ,  B82Y15/00 ,  C03C4/0007 ,  C03C14/008 ,  C07B2200/11 ,  C07H21/00 ,  C12Q1/6816 ,  C40B40/00 ,  G01N33/532 ,  G01N33/54313 ,  G01N33/5432 ,  G01N33/54373 ,  G01N33/552 ,  G01N33/583 ,  G01N33/586 ,  G01N2333/11 ,  G01N2333/195 ,  G01N2333/916 ,  G01N2333/918 ,  G01N2333/924 ,  G01N2405/06 ,  G01N2405/10 ,  C12Q2563/161

摘要： The present invention relates to methods and compositions for the direct detection of analytes and membrane conformational changes through the detection of color changes in biopolymeric materials. In particular, the present invention provide for the direct colorimetric detection of analytes using nucleic acid ligands at surfaces of polydiacetylene liposomes and related molecular layer systems.

摘要翻译： 本发明涉及通过检测生物聚合材料中颜色变化来直接检测分析物和膜构象变化的方法和组合物。 特别地，本发明提供了在聚二乙炔脂质体和相关分子层系统的表面使用核酸配体的分析物的直接比色检测。

公开(公告)号：US20180246112A1

公开(公告)日：2018-08-30

申请号：US15908500

申请日：2018-02-28

申请人： University of Kentucky Research Foundation

发明人： Andrew N. Lane ,  Teresa W. Fan ,  Richard M. Higashi

IPC分类号： G01N33/574 ,  G06F19/12

CPC分类号： G01N33/57488 ,  G01N33/57415 ,  G01N33/57423 ,  G01N2405/02 ,  G01N2405/04 ,  G01N2405/06 ,  G16B5/00

摘要： Provided herein are methods of detecting lipids in humans suspected of having cancer, in particular detecting lipids in samples from a human suspected of having breast or lung cancer.

公开(公告)号：US09695462B2

公开(公告)日：2017-07-04

申请号：US14437941

申请日：2013-11-05

申请人： SHIONOGI & CO., LTD.

发明人： Takashi Ono ,  Atsuko Yamamoto ,  Shuhei Shigaki

IPC分类号： C12Q1/44 ,  G01N33/92

CPC分类号： C12Q1/44 ,  G01N33/92 ,  G01N2333/92 ,  G01N2405/06 ,  G01N2500/00 ,  G01N2500/04 ,  G01N2500/20 ,  G01N2560/00

摘要： The present invention is related to a method for evaluation of drug efficacy of a medicine having a therapeutic or preventive effect against a disease related to EL activity wherein phosphatidylinositol or lysophosphatidylinositol is used as an indicator. The present invention is also related to a method for screening an inhibitor of EL activity using phosphatidylinositol and a kit for use in the method.

公开(公告)号：US09341615B2

公开(公告)日：2016-05-17

申请号：US14491557

申请日：2014-09-19

申请人： Nestec S.A.

发明人： Sebastiano Collino ,  Ivan Montoliu Roura ,  Francois-Pierre Martin ,  Philippe Alexandre Guy ,  Serge Andre Dominique Rezzi

IPC分类号： G01N33/49 ,  G01N33/493 ,  G09B19/00 ,  G01N33/92 ,  G01N33/68

CPC分类号： G01N33/49 ,  G01N33/492 ,  G01N33/493 ,  G01N33/6848 ,  G01N33/6893 ,  G01N33/92 ,  G01N2405/06 ,  G01N2570/00 ,  G01N2800/04 ,  G01N2800/50 ,  G01N2800/7042 ,  G01N2800/7095 ,  G09B19/00 ,  G09B19/0092

摘要： Using NMR/MS based metabonomics and targeted lipidomics approaches the inventors have explored the metabolic phenotypes of aging and longevity in a cohort including centenarians, elderly and young adults. The inventors have identified biomarkers for a reduced risk of developing ageing related chronic inflammatory disorders and propose a method of diagnosing a lifestyle that allows delaying and/or avoiding ageing related chronic inflammatory disorders using PC-O 40:1 as biomarker.

摘要翻译： 使用基于NMR / MS的代谢组学和靶向脂质组学方法，发明人已经探索了包括百岁老人，老年人和年轻成年人在内的老龄化和长寿的代谢表型。 本发明人已经鉴定出降低发展与衰老有关的慢性炎症性疾病的风险的生物标志物，并且提出了一种诊断生活方式的方法，该方法允许使用PC-O 40：1作为生物标志物来延迟和/或避免与衰老相关的慢性炎症性疾病。

公开(公告)号：US20160025751A1

公开(公告)日：2016-01-28

申请号：US14875087

申请日：2015-10-05

申请人： ZORA BIOSCIENCES OY

发明人： Reijo Laaksonen ,  Kim Ekroos ,  Reini Hurme ,  Riika Katainen

IPC分类号： G01N33/92

CPC分类号： G01N33/92 ,  G01N2405/04 ,  G01N2405/06 ,  G01N2570/00 ,  G01N2800/324 ,  G01N2800/50 ,  G01N2800/52

摘要： The present invention inter alia provides a method, and use thereof, of predicting severe CVD complications such as AMI or CVD death by detecting the lipid concentrations or lipid ratios of a biological sample and comparing it to a control and has identified specific lipid markers that are more specific and sensitive in predicting these CVD complications than currently utilized clinical markers. Also provided is an antibodies towards said lipids, and the use thereof for predicting, diagnosing, preventing and/or treating CVD complications. The invention additionally relates to kits comprising lipids and/or an antibody thereto, for use in the prediction and/or diagnosis of CVD complications.