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公开(公告)号：US20020034751A1
公开(公告)日：2002-03-21
申请号：US09852555
申请日：2001-05-10
发明人： Kwok-Yung Yuen , Liang Cao
IPC分类号： C12Q001/68 , C07H021/04 , C12N001/15 , C12P021/02
摘要： Disclosed is an Aspergillus fumigatus AFAP1 polypeptide and DNA (RNA) encoding such AFAP1 polypeptide. Also provided is a procedure for producing such polypeptide by recombinant DNA techniques and a procedure for generating antibodies against the polypeptide. Also disclosed is a method of using such polypeptide and the antibodies against it for the diagnosis of deep infections of Aspergillus fumigatus by detecting the presence of the specific antibodies as well as the AFAP1 protein antigen in clinical specimens taken from suspected patients. Also provided are methods of using the AFAP1 DNA(RNA) or protein sequence to identify and to clone its homologous genes from other Aspergillus species. Therefore, the identification of AFAP1 homologous genes from other pathogenic fungi are made possible with this invention. Also described is a therapeutic regimen using the antibodies against Aspergillus infection. Also provided is a method of immunization against the infection of Aspergillosis.
摘要翻译： 披露了烟曲霉AFAP1多肽和编码这种AFAP1多肽的DNA（RNA）。 还提供了通过重组DNA技术产生这种多肽的方法和产生针对多肽的抗体的方法。 还公开了通过检测来自疑似患者的临床标本中特异性抗体以及AFAP1蛋白抗原的存在，使用此类多肽及其抗体来诊断烟曲霉深部感染的方法。 还提供了使用AFAP1 DNA（RNA）或蛋白质序列鉴定和克隆来自其他曲霉属物种的同源基因的方法。 因此，利用本发明可以鉴定其它致病真菌的AFAP1同源基因。 还描述了使用抗曲霉菌感染的抗体的治疗方案。 还提供了针对曲霉病感染的免疫方法。
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2.发明申请Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast 审中-公开制备酵母中发酵产量提高的胰岛素前体和胰岛素前体类似物的方法公开(公告)号：US20010041787A1
公开(公告)日：2001-11-15
申请号：US09740359
申请日：2000-12-19
IPC分类号： C07K014/62 , C07H021/04 , C12N001/15 , C12P021/02
CPC分类号： C07K14/62
摘要： Novel insulin precursors and insulin precursor analogs comprising a connecting peptide (mini C-peptide) of preferably up to 15 amino acid residues and comprising at least one Gly are provided. The precursors can be converted into human insulin or a human insulin analog. The precursors will typically have a distance between B27 (atom CG2) and A1 (atom CA) of less than 5 null.
摘要翻译： 提供了包含优选至多15个氨基酸残基并且包含至少一个Gly的连接肽（小C肽）的新型胰岛素前体和胰岛素前体类似物。 前体可转化为人胰岛素或人胰岛素类似物。 前体通常具有小于5埃的B27（原子CG2）和A1（原子CA）之间的距离。
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公开(公告)号：US20020058309A1
公开(公告)日：2002-05-16
申请号：US09866028
申请日：2001-05-25
申请人： GENENTECH, INC.
发明人： Kevin P. Baker , David Botstein , Dan L. Eaton , Napoleone Ferrara , Ellen Filvaroff , Mary E. Gerritsen , Audrey Goddard , Paul J. Godowski , J. Christopher Grimaldi , Austin L. Gurney , Kenneth J. Hillan , Ivar J. Kljavin , Mary A. Napier , Margaret Ann Roy , Daniel Tumas , William I. Wood
IPC分类号： A61K038/17 , C07K014/705 , C07H021/04 , C12P021/02 , C12N001/21 , C12N005/06 , C12N001/15
CPC分类号： C07K14/47 , A61K38/00 , C07K14/4703 , C07K14/70578 , C07K16/18 , C07K2317/24 , C07K2319/00 , C07K2319/02
摘要： The present invention is directed to secreted and transmembrane polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.
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4.发明申请Multiply transformed koji mold and a method of manufacturing a flavor enhancer using the same 失效倍增转化的曲霉菌和使用其制造风味增强剂的方法公开(公告)号：US20010049118A1
公开(公告)日：2001-12-06
申请号：US09801734
申请日：2001-03-09
申请人： Kikkoman Corporation
IPC分类号： C12P001/00 , A23L001/20 , C12N001/15
摘要： The present invention provides a koji mold having increased protease activity and peptidase activity relative to a parent strain, a method of breeding the koji mold, and a method of manufacturing a flavor enhancer using the koji mold. More specifically, the present invention provides: (1) a koji mold having increased protease activity and peptidase activity relative to a parent strain obtained by transformation using a protease gene and a peptidase gene. (2) a method of breeding the above koji mold which comprises transforming a parent strain of koji mold using a protease gene and a peptidase gene, and then selecting a transformant having higher protease activity and peptidase activity relative to a parent strain. (3) a method of manufacturing a flavor enhancer which comprises allowing a culture product of the above koji mold to act on a protein.
摘要翻译： 本发明提供了相对于亲本菌株具有增加的蛋白酶活性和肽酶活性的曲霉菌，使用该曲霉菌制造风味增强剂的方法。 更具体地，本发明提供：（1）相对于通过使用蛋白酶基因和肽酶基因转化获得的亲本菌株，具有增加的蛋白酶活性和肽酶活性的曲霉。 （2）育种上述曲霉菌的方法，其包括使用蛋白酶基因和肽酶基因转化曲霉的亲本菌株，然后选择相对于亲本菌株具有较高蛋白酶活性和肽酶活性的转化体。 （3）制造风味增强剂的方法，其包括使上述曲霉的培养物作用于蛋白质。
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5.发明申请公开(公告)号：US20010034045A1
公开(公告)日：2001-10-25
申请号：US09816277
申请日：2001-03-23
IPC分类号： C12P021/02 , C07H021/04 , C12N009/02 , C12N009/14 , C12N001/15
CPC分类号： C12N9/16 , C07K14/37 , C12N9/12 , C12Y207/11001 , C12Y301/03016
摘要： Described herein are methods for increasing the amount of protein secreted by a cell. In one case, a cell is provided which contains a heterologous nucleic acid encoding a protein having unfolded protein response modulating activity and a heterologous nucleic acid encoding a protein of interest to be secreted. In one case, the protein having unfolded protein response modulating activity is selected from the proteins selected from the group consisting of HAC1, PTC2 and lRE1. The protein of interest can be any secreted protein such as a therapeutic or an industrial enzyme. For example the protein can be selected from the group consisting of lipase, cellulase, endo-glucosidase H, protease, carbohydrase, reductase, oxidase, isomerase, transferase, kinase, phosphatase, alpha-amylase, glucoamylase, lignocellulose hemicellulase, pectinase and ligninase.
摘要翻译： 本文描述了增加细胞分泌的蛋白质的量的方法。 在一种情况下，提供了包含编码具有未折叠蛋白质应答调节活性的蛋白质的异源核酸和编码目的蛋白质的异源核酸分泌的细胞。 在一种情况下，具有解折叠的蛋白质应答调节活性的蛋白质选自HAC1，PTC2和IRE1中选择的蛋白质。 感兴趣的蛋白质可以是任何分泌蛋白质，例如治疗剂或工业酶。 例如，蛋白质可以选自脂肪酶，纤维素酶，内切葡糖苷酶H，蛋白酶，碳水解酶，还原酶，氧化酶，异构酶，转移酶，激酶，磷酸酶，α-淀粉酶，葡糖淀粉酶，木质纤维素半纤维素酶，果胶酶和木质素酶。
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