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1652 条记录 (耗时 0.029 秒)

    System and method for the production of recombinant proteins
    1.
    发明申请
    System and method for the production of recombinant proteins 审中-公开
    用于生产重组蛋白的系统和方法

    公开(公告)号：US20040265954A1

    公开(公告)日：2004-12-30

    申请号：US10383673

    申请日：2003-03-05

    发明人： Markus Aebi ,  Michael Wacker

    IPC分类号： C12P021/02 ,  C12N001/21 ,  C07K014/195 ,  C07K014/47 ,  C12N009/10

    CPC分类号： C12N9/1048 ,  A61K47/549 ,  C07K16/121 ,  C07K2317/92 ,  C12P21/005

    摘要： A system and a method for the production of recombinant N-glycosylated target proteins. The system comprises a prokaryotic organism (e.g. Escherichia coli) into which is introduced a genetic information encoding for a metabolic apparatus capable of carrying out the requested N-glycosylation of the target protein. Said prokaryotic organism also contains the genetic information required for the expression of one or more recombinant target proteins. The metabolic apparatus preferably comprises specific glycosyltransferases for the assembly of the oligosaccharide on a lipid carrier and an OTase that covalently links this oligosaccharide to specific residues of the desired protein.

    摘要翻译： 用于生产重组N-糖基化靶蛋白的体系和方法。 该系统包含原核生物（例如大肠杆菌），其中引入了编码能够进行靶蛋白所要求的N-糖基化的代谢装置的遗传信息。 所述原核生物体还含有表达一种或多种重组靶蛋白所需的遗传信息。 代谢装置优选包含用于在脂质载体上装配寡糖的特异性糖基转移酶和共价连接该寡糖与所需蛋白质的特定残基的OTase。

    Novel compounds
    2.
    发明申请
    Novel compounds 失效
    新型化合物

    公开(公告)号：US20040265330A1

    公开(公告)日：2004-12-30

    申请号：US10889335

    申请日：2004-07-12

    申请人： SmithKline Beecham Biologicals S.A.

    发明人： Jean-Louis Ruelle

    IPC分类号： C12Q001/68 ,  C07H021/04 ,  A61K039/02 ,  C07K014/195 ,  C12N001/21 ,  C12N015/74

    CPC分类号： C07K14/22

    摘要： The invention provides BASB047, BASB054, BASB068 and BASB069 polypeptides, and polynucleotides encoding BASB047, BASB054, BASB068 and BASB069 polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are diagnostic, prophylactic and therapeutic uses.

    摘要翻译： 本发明提供了BASB047，BASB054，BASB068和BASB069多肽，以及编码BASB047，BASB054，BASB068和BASB069多肽的多核苷酸以及通过重组技术产生此类多肽的方法。 还提供诊断，预防和治疗用途。

    Process for preparing pravastatin sodium
    3.
    发明申请
    Process for preparing pravastatin sodium 失效
    制备普伐他汀钠的方法

    公开(公告)号：US20040259216A1

    公开(公告)日：2004-12-23

    申请号：US10490937

    申请日：2004-06-01

    发明人： Nam-Hee Choi ,  Kon-Tae Tak ,  Ki-Woo Lee ,  Nam-Hyun Kim ,  Jong-Chang Jun ,  Yoon-Jeong Kong ,  Kyung-Mi Lee

    IPC分类号： C12P017/06 ,  C12P007/62 ,  C12N001/21

    CPC分类号： C12R1/465 ,  C12P7/62 ,  C12P17/06

    摘要： The present invention relates to a process for preparing pravastatin sodium. More specifically, in the process for preparing pravastatin sodium by adding a precursor of the pravastatin to a microorganism-culturing medium to obtain pravastatin sodium, the precursor of the pravastatin is added to a culture medium that is culturing Streptomyces carbophilus KBT229 (KCCM-10317), which is obtained by UV-mutating a Streptomyces carbophilus FERM BP-1145, and cultured to obtain the pravastatin sodium with high density and high efficiency.

    摘要翻译： 本发明涉及制备普伐他汀钠的方法。 更具体地说，在通过向微生物培养基中添加普伐他汀的前体而获得普伐他汀钠制备普伐他汀钠的方法中，将普伐他汀的前体加入到培养链霉菌KBT229（KCCM-10317）的培养基中， 通过UV链霉菌FERM BP-1145的扩增获得，培养，得到高密度，高效率的普伐他汀钠。

    Methods and construction and screening of libraries of chemokine variants
    4.
    发明申请
    Methods and construction and screening of libraries of chemokine variants 失效
    趋化因子变体文库的方法和构建与筛选

    公开(公告)号：US20040258659A1

    公开(公告)日：2004-12-23

    申请号：US10791814

    申请日：2004-03-04

    发明人： Guy Gorochov ,  Oliver Hartley ,  Karim Dorgham ,  Patrice Debre ,  Robin Offord

    IPC分类号： C12P021/02 ,  C07K014/52 ,  C12N001/21

    CPC分类号： C40B40/02 ,  A61K38/04 ,  C07K14/521 ,  C07K14/523 ,  C12N15/1037

    摘要： The present invention provides a method for the design and/or the selection of agonist or antagonist chemokine variants combining a phage display technology and a screening on living cells expressing the receptor of the corresponding native chemokine. It also provides RANTES variants having agonist properties towards said receptor, and methods for preventing and/or curing viral diseases, as well as clues for preventing and/or curing inflammatory or malignant diseases.

    摘要翻译： 本发明提供了一种用于设计和/或选择激动剂或拮抗剂趋化因子变体的方法，其结合噬菌体展示技术和筛选表达相应天然趋化因子的受体的活细胞。 它还提供了具有针对所述受体的激动剂性质的RANTES变体，以及用于预防和/或治疗病毒性疾病的方法，以及用于预防和/或治疗炎性或恶性疾病的线索。

    Novel enone reductases, methods for producing same, and methods for selectively reducing a carbon-carbon double bond of an alpha, beta-unsaturated ketone using the reductases
    5.
    发明申请
    Novel enone reductases, methods for producing same, and methods for selectively reducing a carbon-carbon double bond of an alpha, beta-unsaturated ketone using the reductases 审中-公开
    新型烯酮还原酶，其制备方法以及使用还原酶选择性还原α，β-不饱和酮的碳 - 碳双键的方法

    公开(公告)号：US20040253695A1

    公开(公告)日：2004-12-16

    申请号：US10879250

    申请日：2004-06-29

    申请人： Daicel Chemical Industries, Ltd., a Japanese corporation

    发明人： Hiroaki Yamamoto ,  Norihiro Kimoto

    IPC分类号： C12P007/26 ,  C12N009/04 ,  C12N001/21

    CPC分类号： C12N9/001 ,  C12P7/26

    摘要： The object of the present invention is to provide novel enone reductases useful for producing ketones. Accordingly, novel enone reductases derived from the genus Kluyveromyces are provided by the present invention. In addition, the present invention also provides genes encoding the enzymes and vectors containing the genes, as well as transformants. Furthermore, the present invention provides enone reductases derived from yeast. Methods for selectively reducing the carbon-carbon double bonds of null,null-unsaturated ketones using these enone reductases are provided. Ketones useful as a raw material for pharmaceuticals can be produced based on an enzymatic reaction.

    摘要翻译： 本发明的目的是提供用于生产酮的新的烯酮还原酶。 因此，本发明提供了源自克鲁维酵母属的新型烯酮还原酶。 此外，本发明还提供编码酶的基因和含有该基因的载体以及转化体。 此外，本发明提供衍生自酵母的烯酮还原酶。 提供了使用这些烯酮还原酶选择性还原α，β-不饱和酮的碳 - 碳双键的方法。 可用作药物原料的酮可以基于酶反应产生。

    tdcBC/pckA gene-inactivated microorganism and method of producing L-threonine using the same
    8.
    发明申请
    tdcBC/pckA gene-inactivated microorganism and method of producing L-threonine using the same 有权
    tdcBC / pckA基因灭活的微生物和使用其生产L-苏氨酸的方法

    公开(公告)号：US20040241831A1

    公开(公告)日：2004-12-02

    申请号：US10817044

    申请日：2004-04-02

    发明人： Young Hoon Park ,  Byoung Choon Lee ,  Dae Cheol Kim ,  Jin Ho Lee ,  Jae Yong Cho

    IPC分类号： C12N001/21 ,  C12P013/08

    CPC分类号： C12P13/08 ,  C12N9/88

    摘要： The present invention provide a microorganism comprising an inactivated chromosomal tdcBC gene and an inactivated chromosomal pckA gene, which has remarkably improved productivity of L-threonine. Also, the present invention provides a method of producing L-threonine using the microorganism. The microorganism is prepared by incorporating by a recombination technique an antibiotic resistance gene into a pckA gene on the chromosome of a bacterial strain containing an L-threonine degradation-associated operon gene, tdcBC, which is inactivated. The microorganism has the effect of preventing degradation and intracellular influx of L-threonine due to the inactivation of the tdcBC operon gene, and includes more activated pathways for L-threonine biosynthesis. Therefore, the microorganism is useful for mass production of L-threonine because of being capable of producing L-threonine in high levels and high yields even in the presence of high concentrations of glucose.

    摘要翻译： 本发明提供了包含灭活的染色体tdcBC基因和灭活的染色体pckA基因的微生物，其显着提高了L-苏氨酸的生产率。 另外，本发明提供使用该微生物生产L-苏氨酸的方法。 通过重组技术将抗生素抗性基因并入包含失活的L-苏氨酸降解相关操纵子基因tdcBC的细菌菌株的染色体上的pckA基因中来制备微生物。 该微生物具有防止由于tdcBC操纵子基因失活引起的L-苏氨酸的降解和细胞内流入的作用，并且包括用于L-苏氨酸生物合成的更多活化途径。 因此，即使在高浓度葡萄糖的存在下，微生物也能够以高水平和高产率生产L-苏氨酸，因此可用于批量生产L-苏氨酸。

    Bacterial strain, processed plant extracts, compositions containing same, processes for their preparation and their therapeutic and industrial applications
    10.
    发明申请
    Bacterial strain, processed plant extracts, compositions containing same, processes for their preparation and their therapeutic and industrial applications 失效
    细菌菌株，加工植物提取物，含有它们的组合物，其制备方法及其治疗和工业应用

    公开(公告)号：US20040235662A1

    公开(公告)日：2004-11-25

    申请号：US10432346

    申请日：2003-12-12

    发明人： Mark Olshenitsky ,  Genadi Buchman

    IPC分类号： A01N063/00 ,  A61K035/78 ,  C12N001/21

    CPC分类号： A23K20/10 ,  A23K10/18 ,  A23L21/20 ,  A23L27/84 ,  A23L29/065 ,  A23L33/105 ,  A61K35/644 ,  A61K35/741 ,  A61K36/185 ,  A61K36/23 ,  A61K36/31 ,  A61K36/48 ,  A61K36/534 ,  A61K36/752 ,  A61K36/8962 ,  C12N1/04 ,  C12R1/19 ,  A61K2300/00

    摘要： The present invention discloses: (i) a non-pathogenic probiotic microorganism and its probiotic/therapeutic uses; (ii) a formulation comprising an aqueous solution of a volatile fraction (VF) prepared from the extract of at least one plant derived material and its therapeutic uses; (iii) a process of manufacturing the formulation from the plant derived material; (iv) a probiotic composition comprising the non-pathogenic probiotic microorganism of the invention and/or other probiotic microorganism(s) and the formulation of the invention, and its probiotic/therapeutic uses; (v) a composition for industrial applications comprising the formulation of the invention and microorganism(s) of industrial applicability; and (vi) industrial processes and apparatuses in which the latter composition is used.

    摘要翻译： 本发明公开：（i）非致病性益生菌微生物及其益生菌/治疗用途; （ii）包含由至少一种植物来源材料的提取物制备的挥发性级分（VF）的水溶液及其治疗用途的制剂; （iii）从植物衍生的材料制造制剂的方法; （iv）包含本发明的非致病性益生菌微生物和/或其他益生菌微生物和本发明的制剂及其益生菌/治疗用途的益生菌组合物; （v）用于工业应用的组合物，其包含本发明的制剂和工业适用性的微生物; 和（vi）使用后一种组合物的工业过程和设备。