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    Method for producing L-glutamic acid by fermentation
    2.
    发明申请
    Method for producing L-glutamic acid by fermentation 失效
    通过发酵生产L-谷氨酸的方法

    公开(公告)号：US20030134397A1

    公开(公告)日：2003-07-17

    申请号：US10307320

    申请日：2002-12-02

    申请人： AJINOMOTO CO. INC

    发明人： Wataru Hibino ,  Yasuhiko Yoshihara ,  Masakazu Sugimoto ,  Harufumi Miwa

    IPC分类号： C12P013/14 ,  C12N009/10 ,  C12N001/21

    CPC分类号： C12P13/14

    摘要： A coryneform bacterium which has enhanced intracellular pyruvate carboxylase activity obtained by increasing copy number of a gene encoding the intracellular pyruvate carboxylase, or by enhancing function of a expression regulatory sequence for the gene, and has L-glutamic acid-producing ability is cultured in a medium so that L-glutamic acid should be produced and accumulated in the culture, and L-glutamic acid is collected from the culture.

    摘要翻译： 通过增加编码细胞内丙酮酸羧化酶的基因的拷贝数或通过增强基因的表达调节序列的功能而获得的具有增强的细胞内丙酮酸羧化酶活性并具有L-谷氨酸生产能力的棒状细菌被培养在 培养基中生成L-谷氨酸并在培养物中积累，从培养物中收集L-谷氨酸。

    KIDNEY DISEASE DETECTION AND TREATMENT
    3.
    发明申请
    KIDNEY DISEASE DETECTION AND TREATMENT 失效
    KIDNEY疾病检测和治疗

    公开(公告)号：US20020110799A1

    公开(公告)日：2002-08-15

    申请号：US09415217

    申请日：1999-10-12

    发明人： WAYNE D. COMPER

    IPC分类号： C12Q001/00 ,  C12P013/14

    CPC分类号： G01N33/68 ,  Y10S436/811

    摘要： A method is disclosed for diagnosing early stage of a disease in which an intact protein found in urine is an indicator of the disease. The method includes assaying urine sample to detect the presence of modified protein using either immunological or non-immunological technique. Methods for preventing and treating the disease are also disclosed.

    摘要翻译： 公开了一种用于诊断疾病早期阶段的方法，其中在尿液中发现的完整蛋白质是疾病的指标。 该方法包括使用免疫或非免疫技术测定尿样以检测修饰蛋白的存在。 还公开了预防和治疗疾病的方法。

    MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY AND USES THEREOF
    4.
    发明申请
    MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY AND USES THEREOF 审中-公开
    免疫球蛋白超家族成员及其用途

    公开(公告)号：US20020081625A1

    公开(公告)日：2002-06-27

    申请号：US09417791

    申请日：1999-10-14

    发明人： JOHN FORD ,  GEORGE YEUNG

    IPC分类号： G01N033/53 ,  C07H021/04 ,  C12P013/14 ,  C12N005/02

    CPC分类号： C07K14/70503

    摘要： The present invention provides novel nucleic acids, novel polypeptide sequences encoded by these nucleic acids and uses thereof. The polypeptide sequences were shown to be a novel member of the immunoglobulin superfamily.

    摘要翻译： 本发明提供了新的核酸，由这些核酸编码的新的多肽序列及其用途。 多肽序列显示为免疫球蛋白超家族的新成员。

    COMPOSITIONS AND METHODS FOR DETERMINING ANTI-VIRAL DRUG SUSCEPTIBILITY AND RESISTANCE AND ANTI-VIRAL DRUG SCREENING
    5.
    发明申请
    COMPOSITIONS AND METHODS FOR DETERMINING ANTI-VIRAL DRUG SUSCEPTIBILITY AND RESISTANCE AND ANTI-VIRAL DRUG SCREENING 无效
    用于确定抗病毒药物耐药性和抗药性和抗病毒药物筛选的组合物和方法

    公开(公告)号：US20020034732A1

    公开(公告)日：2002-03-21

    申请号：US09126559

    申请日：1998-07-30

    发明人： DANIEL J. CAPON ,  JEANNETTE M. WHITCOMB ,  NEIL T. PARKIN

    IPC分类号： C12Q001/70 ,  C12Q001/68 ,  C12Q001/18 ,  C12P013/14 ,  C12N001/20 ,  C12N015/00 ,  C12N015/09 ,  C12N015/63 ,  C12N015/70 ,  C12N015/74

    CPC分类号： C07K14/005 ,  C12N15/85 ,  C12N15/86 ,  C12N2770/24222 ,  C12N2840/203 ,  C12N2840/206 ,  C12Q1/6897 ,  C12Q1/707

    摘要： This invention provides a method for determining susceptibility for an HCV or HCMV anti-viral drug comprising: (a) introducing a resistance test vector comprising a patient-derived segment and an indicator gene into a host cell; (b) culturing the host cell from (a); (c) measuring expression of the indicator gene in a target host cell; and (d) comparing the expression of the indicator gene from (c) with the expression of the indicator gene measured when steps (a)-(c) are carried out in the absence of the anti-viral drug, wherein a test concentration of the anti-viral drug is present at steps (a)-(c); at steps (b)-(c); or at step (c). This invention also provides a method for determining HCV or HCMV anti-viral drug resistance in a patient comprising: (a) determining anti-viral drug susceptibility in the patient at a first time using the susceptibility test described above, wherein the patient-derived segment is obtained from the patient at about said time;(b) determining anti-viral drug susceptibility of the same patient at a later time; and (c) comparing the anti-viral drug susceptibilities determined in step (a) and (b) wherein a decrease in anti-viral drug susceptibility at the later time compared to the first time indicates development or progression of anti-viral drug resistance in the patient. This invention also provides a method for evaluating the biological effectiveness of a candidate HCV or HCMV anti-viral drug compound. Compositions including resistance test vectors comprising a patient-derived segment comprising a HCV or HCMV gene and an indicator gene and host cells transformed with the resistance test vectors are provided.

    摘要翻译： 本发明提供了一种用于确定HCV或HCMV抗病毒药物的易感性的方法，包括：（a）将包含患者来源区段和指示基因的抗性测试载体导入宿主细胞; （b）从（a）培养宿主细胞; （c）测量靶宿主细胞中指示基因的表达; 和（d）比较来自（c）的指示基因的表达与在不存在抗病毒药物的情况下进行步骤（a） - （c）时测量的指示基因的表达，其中测试浓度 在步骤（a） - （c）中存在抗病毒药物; 在步骤（b） - （c）; 或在步骤（c）。 本发明还提供了一种用于确定患者HCV或HCMV抗病毒药物耐药性的方法，包括：（a）使用上述敏感性试验在第一时间确定患者的抗病毒药物敏感性，其中所述患者来源的部分 在约所述时间从患者获得;（b）在稍后时间确定相同患者的抗病毒药物敏感性; 和（c）比较步骤（a）和（b）中确定的抗病毒药物敏感性，其中与第一次相比，在稍后时间的抗病毒药物敏感性的降低表明抗病毒药物耐药性的发展或进展 患者。 本发明还提供了评估候选HCV或HCMV抗病毒药物化合物的生物学效力的方法。 提供了包含由包含HCV或HCMV基因的患者来源的片段的抗性测试载体和指示基因以及用抗性测试载体转化的宿主细胞的组合物。

    Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains
    7.
    发明申请
    Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains 有权
    构建产氨酸的细菌菌株的方法，以及用构建的产生氨基酸的菌株通过发酵制备氨基酸的方法

    公开(公告)号：US20040002143A1

    公开(公告)日：2004-01-01

    申请号：US09577005

    申请日：2000-05-25

    发明人： Yoko Asakura ,  Jun Nakamura ,  Sohei Kanno ,  Mikiko Suga ,  Eiichiro Kimura ,  Hisao Ito ,  Kazuhiko Matsui ,  Tsuyoshi Ohsumi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

    IPC分类号： C12P013/04 ,  C12P013/14 ,  C12N001/21 ,  C12N015/74

    CPC分类号： C12P13/14 ,  C12N15/67

    摘要： A method of producing coryneform bacteria having an improved amino acid- or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method can construct a mutant capable of suitably enriching or controling the expression of an intended gene without using a plasmid and also capable of producing amino acids in a high yield, by the recombination or mutation.

    摘要翻译： 具有改善的氨基酸或核酸生产力的棒状细菌的方法包括以下步骤：在棒状细菌的染色体上的氨基酸或核酸 - 生物合成基因的启动子序列中引入突变，使其接近 共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化以使其接近共有序列，以获得棒状杆菌氨基酸的突变体或 产生核酸的微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。 该方法可以构建能够适当地富集或控制预期基因的表达而不使用质粒并且还能够通过重组或突变以高产率产生氨基酸的突变体。

    Nematode GS-like sequences
    8.
    发明申请
    Nematode GS-like sequences 审中-公开
    线虫GS样序列

    公开(公告)号：US20030235898A1

    公开(公告)日：2003-12-25

    申请号：US10446520

    申请日：2003-05-27

    发明人： Andrew P. Kloek ,  Deryck J. Williams ,  Brandy Salmon ,  Merry B. McLaird

    IPC分类号： C12P013/14 ,  C12N009/10 ,  C12N015/74 ,  C12P021/02 ,  C12N005/06 ,  C07K016/40

    CPC分类号： C12N9/93 ,  C12Q1/25 ,  C12Y603/01002 ,  G01N2500/00

    摘要： Disclosed is a nucleic acid molecule from nematodes encoding for glutamine synthetase (GS) polypeptides. The GS-like polypeptide sequence is also provided, as are vectors, host cells, and recombinant methods for production of GS-like nucleotides and polypeptides. The invention further relates to screening methods for identifying inhibitors and/or activators, as well as methods for antibody production.

    摘要翻译： 公开了编码谷氨酰胺合成酶（GS）多肽的线虫的核酸分子。 还提供GS样多肽序列，载体，宿主细胞以及用于产生GS样核苷酸和多肽的重组方法。 本发明还涉及用于鉴定抑制剂和/或激活剂的筛选方法以及用于抗体生产的方法。

    Method for producing L-glutamic acid
    9.
    发明申请
    Method for producing L-glutamic acid 审中-公开
    L-谷氨酸的制备方法

    公开(公告)号：US20030190713A1

    公开(公告)日：2003-10-09

    申请号：US10338915

    申请日：2003-01-09

    申请人： AJINOMOTO CO., INC.

    发明人： Hiroshi Ueda ,  Takayuki Koda ,  Masakazu Sato

    IPC分类号： C12P013/14

    CPC分类号： C12N1/20 ,  C12P13/14

    摘要： A method for producing L-glutamic acid by fermentation, which comprises culturing a microorganism having L-glutamic acid-producing ability in a liquid medium of which pH is adjusted to a condition under which L-glutamic acid produced by the microorganism is allowed to be precipitated, to allow L-glutamic acid to be produced and accumulated with precipitation of L-glutamic acid accompanied, wherein an operation causing existence of L-glutamic acid crystals in the medium is performed when a concentration of L-glutamic acid in the medium is lower than the concentration at which spontaneous crystallization occurs.

    摘要翻译： 一种通过发酵生产L-谷氨酸的方法，其包括在将pH调节至由微生物产生的L-谷氨酸的条件下调节为具有L-谷氨酸生产能力的微生物，使其变为 伴随着L-谷氨酸的沉淀而生成和积累L-谷氨酸，其中当介质中的L-谷氨酸浓度为介质中时，进行介质中存在L-谷氨酸晶体的操作 低于发生自发结晶的浓度。

    TARGET SPECIFIC SCREENS AND THEIR USE FOR DISCOVERING SMALL ORGANIC MOLECULAR PHARMACOPHORES
    10.
    发明申请
    TARGET SPECIFIC SCREENS AND THEIR USE FOR DISCOVERING SMALL ORGANIC MOLECULAR PHARMACOPHORES 审中-公开
    目标特异性筛选及其用于发现小有机分子药物的用途

    公开(公告)号：US20030092057A1

    公开(公告)日：2003-05-15

    申请号：US09412831

    申请日：1999-10-05

    发明人： ARTHUR J. BLUME

    IPC分类号： G01N033/53 ,  G01N033/543 ,  C12P013/14

    CPC分类号： C07K16/2869 ,  C07K16/00 ,  C07K2317/55 ,  C07K2319/00 ,  G01N33/53 ,  G01N33/6854 ,  G01N2500/00

    摘要： The invention relates to a general process by which recombinantly derived variable domains of antibodies encompassing either or both light and heavy variable regions with or without respective constant regions are engineered and selected for identification of unique surface domains of pharmaceutical targets or parts thereof which regulate target function. The recombinant antibodies are useful as reagents for high volume, rapid screening of occupation of the active surface domains by natural or synthetic entities. This invention is also directed to elucidating the three dimensional conformation of the antibodies, or parts thereof, which bind to the pharmaceutical targets and confers activity. Methods for creating high resolution molecular models which can direct the synthesis of biologically active small organic molecules useful as viable discovery drug leads are also provided.

    摘要翻译： 本发明涉及一种通用方法，通过该方法，将包含有或不具有相应恒定区的轻度和重度可变区之一或两者的重组衍生的可变结构域设计和选择用于鉴定调节靶功能的药物靶标或其部分的独特表面结构域 。 重组抗体可用作大量快速筛选天然或合成实体对活性表面结构域的占据的试剂。 本发明还涉及阐明与药物靶标结合并赋予活性的抗体或其部分的三维构象。 还提供了用于产生可以引导用作可行发现药物引物的生物活性小有机分子的合成的高分辨率分子模型的方法。