公开(公告)号：US20040265293A1

公开(公告)日：2004-12-30

申请号：US10807614

申请日：2004-03-24

发明人： John H. Crowe ,  Fern Tablin ,  Nelly M. Tsvetkova

IPC分类号： A01N001/02 ,  A61K045/00

CPC分类号： A61K35/19 ,  A01N1/0221 ,  A01N1/0231 ,  A01N1/0284 ,  A61K45/06 ,  A61K47/6901 ,  Y10T436/108331 ,  A61K2300/00

摘要： A dehydrated composition is provided that includes freeze-dried platelets. The platelets are loaded with trehalose which preserves biological properties during freeze-drying and rehydration. The trehalose loading is conducted at a temperature of from greater than about 25null C. to less than about 40null C., most preferably at 37null C., with the loading solution having trehalose in an amount from about 10 mM to about 50 mM. These freeze-dried platelets are substantially shelf-stable and are rehydratable so as to have a normal response to an agonist, for example, thrombin, with virtually all of the platelets participating in clot formation within about three minutes at 37null C.

摘要翻译： 提供了包含冷冻干燥血小板的脱水组合物。 血小板装载有海藻糖，其在冷冻干燥和补液过程中保留生物学性质。 海藻糖负载在大于约25℃至小于约40℃，最优选在37℃的温度下进行，载体溶液的海藻糖的量为约10mM至约50 mM。 这些冷冻干燥的血小板基本上是货架稳定的并且是可再水合的，以便对激动剂例如凝血酶具有正常的反应，实际上所有的血小板在37℃约3分钟内参与凝块形成。

公开(公告)号：US20040241634A1

公开(公告)日：2004-12-02

申请号：US10449513

申请日：2003-06-02

申请人： Organ Recovery Systems

发明人： Roger Millis ,  John Foley ,  Douglas A. Schein ,  John M. Brassil

IPC分类号： A01N001/02

CPC分类号： A01N1/0247 ,  A01N1/02 ,  F04B43/12 ,  F04B49/022 ,  F04B49/065 ,  F04B2205/05

摘要： A method for controlling a pump for delivery of liquid to an organ over a series of fixed-length time intervals f, each interval f comprising a time t1 and a time t2 wherein t1nullt2 equals the length of interval f. The method comprises allowing output pressure of the pump to decrease over time t1, increasing output pressure of the pump over time t2, comparing achieved pump output pressure to a predetermined pressure at about the end of interval f, and at least one of (i) adjusting t1 and t2 if necessary so the predetermined pressure is approximated by the output pressure at the end of the next interval f, and (ii) adjusting a rate of change of the output pressure during at least one of t1 and t2 if necessary so the predetermined pressure is approximated by the output pressure at the end of the next interval f.

摘要翻译： 一种用于控制用于在一系列固定长度时间间隔f内将液体输送到器官的泵的方法，每个间隔f包括时间t1和时间t2，其中t1 + t2等于间隔f的长度。 该方法包括允许泵的输出压力随着时间t1减小，随着时间t2增加泵的输出压力，将实现的泵输出压力与间隔f的结束时的预定压力进行比较，并且（i） 如果需要，调整t1和t2，使得预定压力由下一个间隔f结束时的输出压力近似，以及（ii）如果需要，在t1和t2中的至少一个期间调整输出压力的变化率， 预定压力由下一个间隔f结束时的输出压力近似。

公开(公告)号：US20040234940A1

公开(公告)日：2004-11-25

申请号：US10481249

申请日：2004-07-19

发明人： Hein Van Der Steen ,  Lalantha R. Abeydeera ,  Jon E. Anderson

IPC分类号： A01N001/02 ,  C12M001/34

CPC分类号： C12M21/06 ,  C12M23/16 ,  C12M23/34 ,  C12M29/04

摘要： A micro chamber arrangement for development of oocytes, fertilisation and embryo culture. Objects are kept in a micro environment. The system monitors and regulates the environment for the objects and incorporates regular quality control. The device is very suitable for large scale embryo production. The arrangement can be used for follicle sorting, follicular growth, oocyte maturation, oocyte enucleation, in vitro fertilisation, cloning, nuclear transfer, genetic modification, embryo culture, embryo encapsulation and embryo transport.

摘要翻译： 用于发育卵母细胞，受精和胚胎培养的微室安排。 物体保存在微环境中。 系统监控和调节对象的环境，并定期进行质量控制。 该装置非常适合大规模胚胎生产。 该装置可用于卵泡分选，滤泡生长，卵母细胞成熟，卵母细胞摘除，体外受精，克隆，核转移，遗传修饰，胚胎培养，胚胎封装和胚胎运输。

公开(公告)号：US20040229204A1

公开(公告)日：2004-11-18

申请号：US10437621

申请日：2003-05-14

发明人： John A. St. Cyr ,  Daniel G. Ericson ,  Clarence A. Johnson

IPC分类号： A01N001/02 ,  A61K031/70

CPC分类号： A01N1/02 ,  A01N1/0226

摘要： D-Ribose, a buffer and an anticoagulant are added to whole blood or packed red cells to extend function in storage beyond 42 days. Methods are disclosed to rejuvenate suboptimally functional red cells. The methods are comprised of incubation of the cells at 37null C. for 10 to 60 minutes in the presence of D-ribose.

摘要翻译： 将D-核糖，缓冲液和抗凝剂加入到全血或包装的红细胞中，以延长储存超过42天的功能。 公开了用于恢复次优功能的红细胞的方法。 所述方法包括在D-核糖存在下在37℃孵育10至60分钟。

公开(公告)号：US20040197765A1

公开(公告)日：2004-10-07

申请号：US10818053

申请日：2004-04-05

发明人： Gordon L. Dorn

IPC分类号： A01N001/02 ,  A61K031/155

CPC分类号： A01N47/44 ,  Y10T436/10 ,  Y10T436/108331 ,  Y10T436/2525 ,  A01N37/40 ,  A01N37/02 ,  A01N2300/00

摘要： A transport/preservation system has been found which provides safe, effective transport for patient specimens and bodily fluids, and functions as a preservative for biological reagents, therapeutics, and personal care products. The transport/preservative formulations can also be used as safe, effective sanitizers on surfaces, equipment, and appliances. The transport preservation formulations comprising a biguanide and one or more other antimicrobial agents are cidal to microorganisms when present in a sample or on a surface to be sanitized.

摘要翻译： 已发现运输/保存系统，为患者标本和体液提供安全，有效的运输，并且用作生物试剂，治疗剂和个人护理产品的防腐剂。 运输/防腐配方也可用作表面，设备和器具上的安全，有效的消毒剂。 包含双胍和一种或多种其它抗微生物剂的运输保存制剂当存在于样品或待消毒的表面时对微生物是污染的。

公开(公告)号：US20040185425A1

公开(公告)日：2004-09-23

申请号：US10659439

申请日：2003-09-11

申请人： National Institute of Agrobiological Sciences ,  Takashi Okuda ,  Masahiko Watanabe ,  Takahiro Kikawada

发明人： Takashi Okuda ,  Masahiko Watanabe ,  Takahiro Kikawada

IPC分类号： A01N001/02

CPC分类号： C12N1/04 ,  A01N1/02 ,  A01N1/0278 ,  A23K50/80

摘要： A tissue of a multicellular organism is gradually dried during cultivation. After the tissue has been completely dehydrated, water is added to the tissue for its recovery. The tissue of the multicellular organism is submerged in an insect body fluid medium treated with heat, and dried for 48 hours or more.

摘要翻译： 多细胞生物体的组织在培养期间逐渐干燥。 在组织完全脱水后，将水加入到组织中进行回收。 将多细胞生物体的组织浸没在用热处理的昆虫体液培养基中，干燥48小时以上。

公开(公告)号：US20040180319A1

公开(公告)日：2004-09-16

申请号：US10807991

申请日：2004-03-23

发明人： Crystal M. Cunanan ,  Lillian J. Quintero ,  Angela de la Fuente ,  Patrice Tremble

IPC分类号： A01N001/02 ,  G01N001/30 ,  G01N033/48

CPC分类号： A61L27/3691 ,  A01N1/00 ,  A61L27/3687 ,  A61L2430/40

摘要： A method for chemical fixation of tissues by exposing the tissue to a chemical fixative agent, under oxidative conditions. The chemical fixative agents useable in this method include aldehydes (e.g., formaldehyde, glutaraldehyde, dialdehyde starch), isocyanates (e.g., hexamethylene diisocyanate) and certain polyepoxy compounds (e.g., DENACOL). The oxidative conditions may be provided by heating of a chemical fixative solution that contains the crosslinking agent, in the presence of room air or oxygen. Alternatively, the oxidative conditions may be provided by adding one or more oxidizing chemicals (e.g., hydrogen peroxide or other peroxides, sodium periodate or other periodates, diisocyanates, halogens, n-bromosuccinimide or other halogenated compounds, permanganates, ozone, chromic acid, sulfuryl chloride, sulfoxides, selenoxides, etc.) to the chemical fixative solution. Alternatively, the oxidative conditions may be provided by irradiation (e.g., alpha, beta, ultraviolet, electron beam, gamma rays) of the fixative solution in the presence of room air or oxygen.

摘要翻译： 一种通过在氧化条件下将组织暴露于化学固定剂的组织化学固定的方法。 在该方法中可用的化学固定剂包括醛（例如甲醛，戊二醛，二醛淀粉），异氰酸酯（例如六亚甲基二异氰酸酯）和某些多环氧化合物（例如DENACOL）。 氧化条件可以通过在室内空气或氧气的存在下加热含有交联剂的化学固定剂溶液来提供。 或者，氧化条件可以通过加入一种或多种氧化性化学物质（例如过氧化氢或其它过氧化物，高碘酸钠或其它高碘酸盐，二异氰酸酯，卤素，正溴代琥珀酰亚胺或其它卤代化合物，高锰酸盐，臭氧，铬酸，磺酰基 氯化物，亚砜，硒氧化物等）到化学固定液中。 或者，可以在室内空气或氧气的存在下通过固定溶液的照射（例如α，β，紫外线，电子束，γ射线）提供氧化条件。

公开(公告)号：US20040157206A1

公开(公告)日：2004-08-12

申请号：US10478198

申请日：2004-04-07

发明人： John Fisher ,  Catherine Booth ,  Eileen Ingham

IPC分类号： A01N001/02

CPC分类号： A61L27/3687 ,  A61L27/3633

摘要： A method of preparing matrices or tissue engineered biomaterials for implantation, and in particular to a method of improving decellularisation of matrices or tissue engineered biomaterials prior to implantation. The method employs a single anionic detergent in combination with protease inhibitors.

摘要翻译： 制备用于植入的基质或组织工程生物材料的方法，特别是在植入之前改进基质或组织工程化生物材料脱细胞化的方法。 该方法采用单一阴离子洗涤剂与蛋白酶抑制剂组合。

公开(公告)号：US20040152066A1

公开(公告)日：2004-08-05

申请号：US10476728

申请日：2003-10-31

发明人： An Tanghe ,  Johan Thevelein ,  Patrick Van Dijck

IPC分类号： A01N001/02 ,  C12N015/85 ,  C12N015/82 ,  C12N015/74

CPC分类号： C12N15/8273 ,  A21D6/001 ,  A21D8/047 ,  C07K14/395 ,  C07K14/705

摘要： The present invention relates to the use of proteins facilitating water diffusion or water transport through the cell membrane, preferably aquaporin or aquaporin related proteins to obtain freeze-tolerant eukaryotic cells, preferably yeast cells or plant cells. It relates further to a method for obtaining such cells, and to freeze-tolerant cells, characterized by an enhanced expression level of proteins facilitating water diffusion or water transport through the cell membrane.

摘要翻译： 本发明涉及促进通过细胞膜，优选水通道蛋白或水通道蛋白相关蛋白的水扩散或水输送的蛋白质的使用，以获得耐冻真核细胞，优选酵母细胞或植物细胞。 它进一步涉及获得这种细胞的方法，以及抗冻细胞，其特征在于促进水扩散或通过细胞膜的水输送的蛋白质的表达水平。

公开(公告)号：US20040146846A1

公开(公告)日：2004-07-29

申请号：US10756368

申请日：2004-01-14

申请人： SIGMA-TAU INDUSTRIE FARMACEUTICHE RIUNITE S.p.A.

发明人： Secondo Dottori

IPC分类号： A01N001/02

CPC分类号： A61L2/0058 ,  A01N1/0215 ,  A01N1/0226 ,  A01N1/0294 ,  A61L2/0035 ,  A61L2/0047 ,  A61L2202/22

摘要： Cell membrane maintenance of red blood cells and platelet concentrates is improved by the addition of 1 mM-10 mM L-carnitine and derivatives. This improvement allows extension of the period of viability of packed red blood cells and platelet concentrations beyond current periods. Additionally, the materials so treated exhibit extended circulation half life upon transfusion to a patient. Improvements in membrane maintenance achieved by this method permit irradiation of sealed containers of blood products so as to substantially sterilize and destroy leukocytes in the same.

摘要翻译： 通过加入1mM-10mM左旋肉碱及其衍生物来改善红细胞和血小板浓缩物的细胞膜维持。 这种改善使得包装的红血细胞和血小板浓度的生存期延长超过当前时期。 此外，如此处理的材料在输血时显示延长的循环半衰期。 通过该方法实现的膜维持改进允许照射血液制品的密封容器，从而基本上消毒和破坏其中的白细胞。