公开(公告)号：US20240360190A1

公开(公告)日：2024-10-31

申请号：US18638496

申请日：2024-04-17

申请人： HENI INNOVATION PRIVATE LIMITED

发明人： Parini Kapadia

IPC分类号： C07K14/47 ,  A23C11/08 ,  C12N1/14 ,  C12N15/80 ,  C12Q1/24 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6895 ,  C12R1/885 ,  G01N33/68

CPC分类号： C07K14/4732 ,  A23C11/08 ,  C12N1/145 ,  C12N15/80 ,  C12Q1/24 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6895 ,  G01N33/6803 ,  C12R2001/885 ,  G01N2333/4731

摘要： The present invention broadly relates to the field of biotechnology. More particularly, the present invention relates to recombinant vector systems for the production of animal free milk proteins and a process for the production of the animal protein in recombinant host cells.

公开(公告)号：US12129464B2

公开(公告)日：2024-10-29

申请号：US18061825

申请日：2022-12-05

申请人： MORAVA INC.

发明人： Mike Mkhitar Moradian

IPC分类号： C12N15/10 ,  C12Q1/6806

CPC分类号： C12N15/1093 ,  C12Q1/6806

摘要： An improved method for Next Generation Sequencing which relies on the presence of the same distinct unique molecular identifier (UMI) located at each end of a linear nucleic acid molecule so that sequence reads of approximately 2 kb or longer are obtained, and which allows generation of a genomic map without the need of a reference sequence.

公开(公告)号：US20240352518A1

公开(公告)日：2024-10-24

申请号：US18682716

申请日：2022-08-12

申请人： The Johns Hopkins University

发明人： Bert Vogelstein ,  Kenneth W. Kinzler ,  Nickolas Papadopoulos ,  Austin Mattox ,  Joshua David Cohen ,  Yuxuan Wang

IPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6844

CPC分类号： C12Q1/6874 ,  C12Q1/6844 ,  C12Q1/6806 ,  C12Q2600/154

摘要： Provided herein are methods for identifying a genetic characteristic, a fragment characteristic and an epigenetic characteristic of a double-stranded DNA molecule in a population of double-stranded DNA molecules by assaying both strands of the double-stranded DNA molecule.

公开(公告)号：US20240344135A1

公开(公告)日：2024-10-17

申请号：US18294652

申请日：2022-08-05

申请人： Agency for Science, Technology and Research ,  Nation University Hospital (Singapore) Pte Ltd

发明人： Lian Chye Winston Koh ,  Peiling Yang

IPC分类号： C12Q1/6886 ,  C12Q1/6806

CPC分类号： C12Q1/6886 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158

摘要： There is provided a method of detecting and/or determining the presence of one or more thyroid-specific nucleic acid, the method comprising annealing the one or more thyroid-specific nucleic acid in the presence of a control nucleic acid, and subjecting each of the one or more thyroid-specific nucleic acid to one or more amplification step in the presence of a mixture comprising a surfactant and an oligonucleotide primer and/or probe capable of hybridizing with the one or more thyroid-specific nucleic acid, wherein the oligonucleotide primer and/or probe comprises a cleavage site and a cleavable 3′ end. Also disclosed are methods of detecting and/or determining thyroid cancer recurrence and/or metastasis and thyroid-specific nucleic acid detection mixtures, and kits thereof.

公开(公告)号：US20240344110A1

公开(公告)日：2024-10-17

申请号：US18294628

申请日：2022-08-01

申请人： INVITROS CO., LTD.

发明人： Min Ji LIM ,  Jan Di KIM ,  Se Ryun KIM ,  Chang Ju PARK

IPC分类号： C12Q1/6806

CPC分类号： C12Q1/6806

摘要： The present invention relates to a composition for cell lysis and nucleic acid extraction, a nucleic acid extraction method using the same, and a molecular diagnostic method using the same, and specifically, to a composition for cell lysis and nucleic acid extraction, a nucleic acid extraction method using the same, and a molecular diagnostic method using the same, wherein a composition containing an RNase inhibitor is used as a solution for nucleic acid extraction, and steps of heating to specific temperatures are included, whereby it is possible to minimize the time for molecular diagnosis by performing polymerase chain reaction without a separate elution process and purification process, and to reduce the cost of molecular diagnosis by minimizing the use of dedicated devices and consumables in extraction.

公开(公告)号：US20240341300A1

公开(公告)日：2024-10-17

申请号：US18579677

申请日：2022-07-15

申请人： SiO2 Medical Products, Inc.

发明人： Joseph Piccirilli ,  Christopher Weikart ,  Alexander M. Klibanov ,  Tia Hexom ,  Brandy Nunez ,  Robert S. Abrams

IPC分类号： A01N1/02 ,  C12Q1/6806

CPC分类号： A01N1/0226 ,  C12Q1/6806

摘要： This disclosure is directed to nucleic acid and cell preservative compositions. Methods of preserving nucleic acids and/or cells in a blood or other biological sample, and kits for preserving nucleic acids and/or cells in a blood or other biological sample are also described.

公开(公告)号：US12116640B2

公开(公告)日：2024-10-15

申请号：US18436821

申请日：2024-02-08

申请人： GUARDANT HEALTH, INC.

发明人： Stefanie Ann Ward Mortimer ,  AmirAli Talasaz ,  Darya Chudova ,  Helmy Eltoukhy

IPC分类号： C12Q1/6886 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  G01N33/574 ,  G16B20/20 ,  G16B30/10 ,  G16H50/20 ,  G16H50/30

CPC分类号： C12Q1/6886 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  G01N33/57407 ,  G16B20/20 ,  G16B30/10 ,  G16H50/20 ,  G16H50/30 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/166

摘要： Disclosed herein are methods, compositions, and devices for use in the early detection of cancer. The methods include preparing cell-free nucleic acid molecules from a subject for sequencing, sequencing a panel of regions in the cell-free nucleic acid molecules, and detecting one or more markers that are indicative of a cancer.

公开(公告)号：US20240339216A1

公开(公告)日：2024-10-10

申请号：US18579487

申请日：2022-07-14

申请人： The Regents of the University of California

发明人： Gregory Poore

IPC分类号： G16H50/20 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q1/6895 ,  G16B30/10 ,  G16H50/70

CPC分类号： G16H50/20 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q1/6895 ,  G16B30/10 ,  G16H50/70 ,  C12Q2600/112 ,  C12Q2600/154

摘要： Methods and systems are presented herein for predicting cancer of a subject through a combination of fungal and non-fungal features of a biological sample. Some embodiments, describe a method of predicting cancer of a subject from a combined fungal and non-fungal microbial presence of a biological sample by: detecting a fungal presence and a non-fungal microbial presence in a sample, removing contaminating fungal features of the fungal presence and contaminating non-fungal microbial features of the non-fungal microbial presence, and predicting a cancer of the subject by correlating the combined decontaminated fungal presence and the decontaminated non-fungal microbial presence to a known combined fungal presence and non-fungal microbial presence for one or more cancers.

公开(公告)号：US20240336959A1

公开(公告)日：2024-10-10

申请号：US18034595

申请日：2021-12-17

申请人： PIXELGEN TECHNOLOGIES AB

发明人： Simon FREDRIKSSON ,  Filip KARLSSON

IPC分类号： C12Q1/6841 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6876

CPC分类号： C12Q1/6841 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6876

摘要： Provided herein is a method for making a physical map of a population of barcoded particles. In some embodiments, the method may involve: producing a complex comprising: i. a population of barcoded particles, wherein the barcoded particles are uniquely barcoded by surface-tethered oligonucleotides that have unique particle identifier sequences; and ii. a population of bridging moieties that comprises oligonucleotide sequences; wherein the bridging moieties are hybridized directly or indirectly to complementary sites in the surface-tethered oligonucleotides; performing a ligation, polymerization and/or a gap-fill/ligation reaction on the complex, thereby producing reaction products that comprise pairs of unique particle identifier sequences or complements thereof from adjacent barcoded particles: sequencing the reaction products, analyzing the sequences to making one or more physical maps of the barcoded particles. Systems for practicing the method are also provided.

公开(公告)号：US20240334924A1

公开(公告)日：2024-10-10

申请号：US18579616

申请日：2022-07-20

申请人： SEKISUI MEDICAL CO., LTD.

发明人： Takaya UCHIYAMA ,  Kuniya KOMAI ,  Tomonori INOUE

IPC分类号： A01N1/02 ,  C12Q1/6806

CPC分类号： A01N1/021 ,  A01N1/0242 ,  A01N1/0284 ,  C12Q1/6806

摘要： Provided is a storage solution for a cell-containing solution capable of suppressing leakage of genomic DNA to the outside of cells when a mixed solution of a cell-containing solution and a storage solution is stored under refrigeration. A storage solution for a cell-containing solution according to the present invention contains a formaldehyde donor compound (A) and a compound (B) that is an inorganic salt, an organic acid, or an organic salt, an electrical conductivity of the storage solution being 3 ms/cm or more and 20 ms/cm or less.