NOVEL PROTEIN AND PROCESS FOR PRODUCING THE SAME
    5.
    发明申请
    NOVEL PROTEIN AND PROCESS FOR PRODUCING THE SAME 审中-公开
    新型蛋白质及其生产方法

    公开(公告)号:WO1996033215A1

    公开(公告)日:1996-10-24

    申请号:PCT/JP1996001062

    申请日:1996-04-19

    CPC classification number: C07K14/51 A61K38/00 Y10S514/801

    Abstract: A protein comprising an amino acid sequence represented by SEQ ID NO: 1 in the Sequence Listing and originating in human MP52 and the dimer of this protein. This dimer protein can be obtained by constructing a plasmid containing a DNA wherein a codon encoding methionine is added to the 5' end of a DNA sequence encoding the above-mentioned aminoacid sequence, transforming Escherichia coli by this plasmid, incubating the E. coli transformant, solubilizing and purifying the obtained inclusion body to thereby give a monomer protein, and then renaturing the obtained monomer protein into the dimer followed by purification. This dimer protein is useful in the treatment of cartilage and bone diseases.

    Abstract translation: 包含序列表中SEQ ID NO:1所示的氨基酸序列并且源于人MP52的蛋白质和该蛋白质的二聚体的蛋白质。 该二聚体蛋白质可以通过构建含有DNA的质粒来获得,其中将编码甲硫氨酸的密码子加入编码上述氨基酸序列的DNA序列的5'末端,通过该质粒转化大肠杆菌,将大肠杆菌转化体 溶解并纯化得到的包含体,从而得到单体蛋白质,然后将得到的单体蛋白质复性成二聚体,然后纯化。 该二聚体蛋白可用于治疗软骨和骨骼疾病。

    COFFEE EXTRACTING APPARATUS
    6.
    发明申请
    COFFEE EXTRACTING APPARATUS 审中-公开
    咖啡提取装置

    公开(公告)号:WO1997007722A1

    公开(公告)日:1997-03-06

    申请号:PCT/JP1996000715

    申请日:1996-03-15

    Inventor: ENOMOTO, Koichi

    CPC classification number: A47J31/053 A47J31/002 A47J31/0573

    Abstract: A coffee extracting apparatus comprises an extractor (20) for a coffee liquid, a receiver-decanter (30) capable of holding water for extraction, receiving the coffee liquid into a cup or the like, a circulation passage (50) and a circulating pump (60) for conveying a liquid in the receiver-decanter (30) and pouring the same into the extractor (20), a circulating water heater (70), and a control unit (90). The control unit (90) drives the circulating pump (60) for a predetermined period of time (T1) for circulating of the liquid when a water supply and extraction switch (11) is on, and turns on the circulation water heater (70) for a limited period of time (T0) in the beginning of operation whereby heated water of high temperature is poured for the limited period of time (T0) in the beginning of operation and thereafter non-heated water is poured.

    Abstract translation: 一种咖啡提取装置,包括用于咖啡液的提取器(20),能够容纳提取水的接收器滗析器(30),将咖啡液体接收到杯子等中,循环通道(50)和循环泵 (60),用于输送接收器滗析器(30)中的液体并将其倾倒到提取器(20)中,循环热水器(70)和控制单元(90)。 控制单元(90)在供水和取出开关(11)打开时将循环泵(60)驱动预定时间段(T1)以循环液体,并打开循环热水器(70) 在操作开始时的有限时间段(T0),其中高温的加热水在操作开始时被注入有限的时间段(T0),然后倒入非加热的水。

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