Abstract:
Soil bacteria can be isolated which produce an enzyme capable of catalyzing the degradation of mannan-containing hemicellulose under conditions combining high pH and high temperature. Such bacteria can be cultured or used as sources of genetic information with which to engineer other microorganisms to produce the enzyme. Commercially useful quantities of native or recombinant hemicellulase can thus be produced by cultures consisting essentially of microorganisms capable of producing the enzyme.
Abstract:
The invention discloses a recombinant polypeptide having mannanase activity, methods for its production, a recombinant host cell producing mannanase, and it's uses in detergents, mannan degradation, feeds and oil drilling.
Abstract:
The present compositions and methods relate to a beta-mannanase from Mahella australiensis , polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).
Abstract:
A frozen confection comprising citrus fibre, characterised in that the citrus fibre has been treated with a glycosidase enzyme is provided. A process for the production of a frozen confection comprising the steps of: a) contacting citrus fibre with a glycosidase enzyme; b) combining the citrus fibre with the other ingredients of the frozen confection to form a mix; c) homogenising the mix at a pressure of from 50 to 500 bar; and d) freezing the mix to form the frozen confection, wherein step a) can take place before, during or after step b) is also provided.
Abstract:
The invention is directed to enzymes having a glycosidase activity, including thermostable and thermotolerant glycosidase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The glycosidase activities of the enzymes of the invention include the hydrolysis of any glycosidic linkages. For example, a glycosidase activity of an enzyme of the invention includes hydrolysis of a beta-anomeric configuration of a glycosidic linkage, such as the hydrolysis of beta-glucosides, beta-fucosides and beta-galactosides. Another glycosidase activity of an enzyme of the invention includes alpha-galactosidase activity, e.g., catalysis of the hydrolysis of galactose groups on a polysaccharide backbone or catalyzing the hydrolysis of the cleavage of di- or oligosaccharides comprising galactose. Another glycosidase activity of an enzyme of the invention includes catalysis of the hydrolysis of mannose groups internally on a polysaccharide backbone or hydrolysis of the cleavage of di-or oligosaccharides comprising mannose groups. Beta-mannosidases of the invention can hydrolyze non-reducing, terminal mannose residues on a mannose-containing polysaccharide and catalyze the cleavage of di- or oligosaccharides comprising mannose groups.
Abstract:
Novel mannanases comprising e.g. an amino acid sequence as shown in positions 31-330 of SEQ ID NO: 2 or their homologues may be derived from e.g. Bacillus sp. 1633, or may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO: 1 from nucleotide 91 to nucleotide 990, polynucleotide molecules that encode a polypeptide that is at least 65 % identical to the amino acid sequence of SEQ ID NO: 2 from amino acid residue 31 to amino acid residue 330, or degenerate nucleotide sequences thereof. The mannanases are alkaline and are useful e.g. in cleaning compositions, in a fracturing fluid useful to fracture a subterranean formation, for modifying plant material, and for treatment of cellulosic fibres.
Abstract translation:新型甘露聚糖酶 SEQ ID NO:2的位置31-330所示的氨基酸序列或其同源物可以衍生自例如SEQ ID NO: 芽孢杆菌 1633,或者可以由包含核苷酸91至核苷酸990的SEQ ID NO:1所示的核苷酸序列的多核苷酸分子编码,编码与SEQ ID NO的氨基酸序列至少65%相同的多肽的多核苷酸分子 :2个氨基酸残基31至氨基酸残基330,或其简并核苷酸序列。 甘露聚糖酶是碱性的,并且是有用的。 在清洁组合物中,在用于破坏地层的压裂液中,用于修饰植物材料和用于纤维素纤维的处理。
Abstract:
A process for increasing the viscosity or gel strength of certain food products is disclosed. By the process a pectinaceous homogenate or slurry is subjected to: a) a treatment with a mixture of enzymes, comprising one or more among galactanases, arabinanases, alpha -arabinofuranosidases, rhamnogalacturonan acetyl esterases (RGAE), endoglucanases, mannanases, xylanases, and proteolytic enzymes; b) a treatment with a pectinesterase (PE), which PE is essentially free from pectic depolymerizing enzymes; followed by c) an enzyme inactivating treatment; said process being performed in the presence of divalent metal ions, especially Ca , said ions being inherently present in said homogenate or slurry, or said ions being added at any time prior to, during or after the process steps (a) to (c). The final product may be a jam, marmalade, jelly, juice, paste, soup, dressing, sauce, condiment, ketchup, salsa, chutney, pudding, mousse, or other dessert.
Abstract:
The present invention relates to polypeptides having mannanase activity, catalytic domains, and carbohydrate binding modules, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding modules.
Abstract:
The present compositions and methods relate to a beta-mannanase from Mahella australiensis, polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).
Abstract:
The present compositions and methods relate to a beta-mannanase from Paenibacillus polymyxa , polynucleotides encoding the beta-mannanase, and methods of making and/or using thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).