Abstract:
A fermentation process using hemicellulose-derived carbohydrates for the production of cellulase mixtures with a high proportion of cellulases relative to hemicellulases is provided. The cellulases produced by the process of the invention are further characterized by high specifi productivity. The resulting cellulase mixtures comprise at least two times more cellulase than hemicellulase and are useful for the hydrolysis of cellulosic substrated, particularly, pretreated lignocellulosic substrate.
Abstract:
The production of interleukin-24 (IL-24) cytokine in plants is described. A plant optimized nucleic acid molecule encoding a IL-24 polypeptide is disclosed. Also described are genetic constructs comprising a regulatory region operably linked to a plant optimized nucleic acid molecule encoding a IL-24 polypeptide. The regulatory region may be an inducible promoter. Also disclosed are methods of transforming a plant, portion thereof, or plant cell with the genetic construct and method of using a plant, a portion of a plant or a plant cell that expresses IL-24.
Abstract:
A method for producing one or more than one isoflavonoid compound in a plant is described. The method comprises providing a production plant comprising one or more than one nucleotide sequence encoding isoflavone glycosyltransferase operatively linked to a heterologous promoter, isoflavone malonyltransferase operatively linked to a heterologous promoter, or both isoflavone glycosyltransferase operatively linked to a heterologous promoter and isoflavone malonyltransferase operatively linked to a heterologous promoter. The one or more than one nucleotide sequence is expressed in the production plant, to produce the one or more than one isoflavonoid compound. Preferably, the production plant contains all the other necessary enzymes of isoflavonoid biosynthesis to produce the one or more than one isoflavonoid compound.
Abstract:
A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
Abstract:
The present invention provides for methods of purifying silicon, methods for obtaining purified silicon, as well as methods for obtaining purified silicon crystals, purified granulized silicon and/or purified silicon ingots.
Abstract:
Advantages such as reduced emissions of NOx can be achieved if engines are fuelled with mixtures of gaseous fuels such as hydrogen and natural gas. It is also desirable to store the gaseous fuels separately so that the fuel mixture ratio can be changed responsive to engine operating conditions. However, compared to liquid fuels, a problem with storing gaseous fuels is that they generally require larger storage volumes to hold an equivalent amount of fuel on an energy basis. There are also obstacles to storing some gaseous fuels such as hydrogen in liquefied form because of the energy required to liquefy them and the extremely low temperatures needed to store such gaseous fuels in liquefied form. The invention relates to an apparatus for increasing the storage density of gaseous fuels such as hydrogen by storing them in gaseous form at high pressures and at sub-ambient temperatures. An apparatus is provided for separately storing a first gaseous fuel and a second gaseous fuel. A first vessel defines a first thermally insulated space for holding the first gaseous fuel in a liquefied form. A second thermally insulated space that can hold the second fuel is disposed within the first vessel. The second thermally insulated space is separated from the first thermally insulated space by a thermally conductive fluid barrier. The second gaseous fuel liquefies at a lower temperature than the first gaseous fuel, whereby the second gaseous fuel can be stored within the second thermally insulated space in a gaseous form at a sub-ambient temperature.
Abstract:
An apparatus and method are provided for pumping a cryogenic fluid from a storage vessel. The cryogenic fluid is pumped from the storage vessel to a heat exchanger and then to a delivery conduit. A pressure sensor measures fluid pressure in the delivery conduit. An electronic controller is programmed to monitor a signal from the pressure sensor, process the signal to determine from the measured process fluid pressure when cryogenic pump performance is degraded, and to send a signal to an operator of the apparatus indicating when the electronic controller determines that cryogenic pump performance has degraded below a predetermined threshold volumetric efficiency.
Abstract:
The invention may be summarized as follows. A combination to be parenterally delivered to a critically ill patient or for the purpose of improving mitochondrial function. The combination comprises a glutamine precursor molecule and an antioxidant in sufficient concentrations to be clinically effective. The combination may be prepared in the absence of lipids or carbohydrates. The combination may be prepared in small volumes to benefit volume restricted patients. A composition, or a unit dosage form comprising the combination and methods of administering the combination, composition or unit dosage form are also provided.
Abstract:
A solid composition stick applicator is provided. The applicator comprises an applicator body having a first and second end, the second end having an opening through which a solid composition stick is dispensed. Within the applicator is housed a helical tension spring having a first spring end and a second spring end, the first spring end, second spring end, or both the first and second spring end held at a fixed position with respect to the applicator body. The helical tension spring is wound around a guide system that is housed within the applicator body. A pushing assembly is attached to the helical tension spring and the pushing assembly is movable from a first position at or near the first end of the applicator body, to a second position at or near the second end of the applicator body. Tension within the helical tension spring is greater in the first position than in the second position thereby urging the pushing assembly towards the -second end of the applicator body. The use of a helical tension spring within the applicator greatly increases the life of the applicator under field conditions.
Abstract:
A fuel injection valve is provided for introducing a fuel into an engine and controlling fuel flow to reduce variability between injection events. The fuel injection valve employs an arrangement for a valve nozzle that cooperates with a valve needle to provide a range of needle movement within which the fuel mass flow rate is substantially constant. This can be achieved by providing a restriction with a constant flow area for a predetermined range of needle movement. The method comprises commanding a valve needle to a position within the predetermined range of needle movement to reduce variability in the fuel mass flow rate, particularly when the engine is idling or operating under low load conditions. Valve needle lift is variable during an injection event and from one injection event to another injection event.