公开(公告)号：WO03014317A3

公开(公告)日：2003-10-16

申请号：PCT/US0225191

申请日：2002-08-08

Applicant: CELMED BIOSCIENCES USA

Inventor： NEUMAN TOOMAS ,  LEVESQUE MICHEL

IPC: A61K35/12 ,  C12N5/0793 ,  C12N5/00 ,  A01N63/00 ,  A61K48/00

CPC classification number: C12N5/0619 ,  A61K35/12 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/13 ,  C12N2501/23 ,  C12N2501/385 ,  C12N2501/40

Abstract: The invention is directed to the field of human stem cells and includes methods and compositions for isolating, propagating, and differentiating human stem cells. The invention provides therapeutic uses of the methods and compositions, including autologous transplantation of treated cells into humans for treatment of Parkinson's and other neuronal disorders.

Abstract translation: 本发明涉及人干细胞领域并且包括用于分离，增殖和分化人干细胞的方法和组合物。 本发明提供了所述方法和组合物的治疗用途，包括将治疗细胞自体移植入人体以治疗帕金森病和其他神经元病症。

公开(公告)号：WO0204605A3

公开(公告)日：2003-02-27

申请号：PCT/US0121793

申请日：2001-07-10

Applicant: SOUND PHARMACEUTICALS INC

Inventor： KIL JONATHAN ,  GU RENDU ,  GRIGEUR CORINNE ,  LOWENHEIM HUBERT

IPC: C12N15/09 ,  A61K31/282 ,  A61K31/519 ,  A61K31/711 ,  A61K33/14 ,  A61K33/24 ,  A61K38/00 ,  A61K38/17 ,  A61K45/00 ,  A61K45/06 ,  A61K48/00 ,  A61P27/16 ,  A61P43/00 ,  C12N5/0793 ,  C12N5/06

CPC classification number: A61K38/1709 ,  C12N5/062 ,  C12N2501/40 ,  C12N2501/60

Abstract: The present invention provides methods for stimulating the formation of inner ear cells, including inner ear sensory hair cells and inner ear support cells. The methods of the present invention damage and/or kill inner ear cells, and stimulate the formation of new, inner ear cells.

Abstract translation: 本发明提供刺激内耳细胞形成的方法，包括内耳感觉毛细胞和内耳支持细胞。 本发明的方法损伤和/或杀死内耳细胞，并刺激新的内耳细胞的形成。

公开(公告)号：WO02004605A2

公开(公告)日：2002-01-17

申请号：PCT/US2001/021793

申请日：2001-07-10

IPC: C12N15/09 ,  A61K31/282 ,  A61K31/519 ,  A61K31/711 ,  A61K33/14 ,  A61K33/24 ,  A61K38/00 ,  A61K38/17 ,  A61K45/00 ,  A61K45/06 ,  A61K48/00 ,  A61P27/16 ,  A61P43/00 ,  C12N5/0793 ,  C12N5/00

CPC classification number: A61K38/1709 ,  C12N5/062 ,  C12N2501/40 ,  C12N2501/60

Abstract: The present invention provides methods for stimulating the formation of inner ear cells, including inner ear sensory hair cells and inner ear support cells. The methods of the present invention damage and/or kill inner ear cells, and stimulate the formation of new, inner ear cells.

Abstract translation: 本发明提供刺激内耳细胞形成的方法，包括内耳感觉毛细胞和内耳支持细胞。 本发明的方法损伤和/或杀死内耳细胞，并刺激新的内耳细胞的形成。

公开(公告)号：WO2016044721A1

公开(公告)日：2016-03-24

申请号：PCT/US2015/050933

申请日：2015-09-18

Applicant: REGENERATIVE MEDICAL SOLUTIONS, INC.

Inventor： XU, Xiaofang ,  ODORICO, Jon ,  FORSBERG, Erik ,  MAEL, Amber A.

IPC: C12N5/00 ,  C12N5/02

CPC classification number: C12N5/0676 ,  A61K9/1652 ,  A61K9/5036 ,  A61K35/39 ,  A61K35/545 ,  A61K45/06 ,  C12N2500/05 ,  C12N2500/22 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/33 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/40 ,  C12N2501/727 ,  C12N2501/91 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  G01N33/507

Abstract: Methods, kits, compositions, and systems are provided for culturing pluripotent stem cells to produce populations of cells comprising beta-like cells (e.g., pancreatic lineage, glucose-responsive, and/or insulin-producing). In particular, culture conditions are provided that result in the generation of beta-like 5 cells from a starting culture of human pluripotent stem cells.

Abstract translation: 提供了用于培养多能干细胞以产生包含β样细胞（例如胰腺谱系，葡萄糖反应性和/或产生胰岛素）的细胞群的方法，试剂盒，组合物和系统。 特别地，提供导致从人多能干细胞的起始培养产生β样5细胞的培养条件。

公开(公告)号：WO2015052143A1

公开(公告)日：2015-04-16

申请号：PCT/EP2014/071363

申请日：2014-10-06

Applicant: CAMBRIDGE ENTERPRISE LIMITED

Inventor： VALLIER, Ludovic ,  HANNAN, Nicholas

IPC: C12N5/071

CPC classification number: C12N5/068 ,  C12N5/0672 ,  C12N5/0678 ,  C12N5/0689 ,  C12N2500/92 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/40 ,  C12N2501/415 ,  C12N2501/91 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  G01N33/5023

Abstract: This invention relates to the differentiation of pluripotent cells (PSCs) into foregut stem cells (FSCs) using a definitive endoderm induction medium comprising a TGFfi ligand, fibroblast growth factor (FGF), bone morphogenetic protein (BMP) and a PI3K inhibitor to differentiate the pluripotent cells into definitive endoderm cells and a foregut induction medium comprising a TGFβ ligand to differentiate the definitive endoderm cells into foregut stem cells (FSCs). Methods of differentiation, populations of foregut stem cells, culture media and kits are provided.

Abstract translation: 本发明涉及使用包含TGFfi配体，成纤维细胞生长因子（FGF），骨形态发生蛋白（BMP）和PI3K抑制剂的确定性内胚层诱导培养基将多能细胞（PSC）分化为前肠干细胞（FSC），以区分 多能细胞进入确定的内胚层细胞和包含TGFβ配体的前肠诱导培养基，以将定形内胚层细胞分化为前肠干细胞（FSC）。 提供分化方法，前肠干细胞群，培养基和试剂盒。

公开(公告)号：WO2015026947A1

公开(公告)日：2015-02-26

申请号：PCT/US2014/051899

申请日：2014-08-20

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： BROOKS, George A. ,  HUSSIEN, Rajaa ,  RIHN, Bertrand

IPC: G01N33/58

CPC classification number: C12N5/0618 ,  B82Y5/00 ,  C07K1/145 ,  C12N5/0018 ,  C12N5/0693 ,  C12N2500/84 ,  C12N2501/10 ,  C12N2501/40 ,  C12N2533/30 ,  G01N33/5044 ,  G01N33/6848 ,  G01N2560/00 ,  G01N2570/00 ,  Y10S977/773 ,  Y10S977/923

Abstract: The present disclosure provides compositions for in vitro culture of cells. The present disclosure provides methods for in vitro culture of cells. The present disclosure provides methods for extracting proteins from a serum sample.

Abstract translation: 本公开提供用于体外培养细胞的组合物。 本公开提供了用于体外培养细胞的方法。 本公开提供了从血清样品中提取蛋白质的方法。

公开(公告)号：WO2015002724A3

公开(公告)日：2015-02-26

申请号：PCT/US2014041988

申请日：2014-06-11

Applicant: HARVARD COLLEGE

Inventor： PETERSON QUINN P ,  PAGLIUCA FELICIA J ,  MELTON DOUGLAS A ,  MILLMAN JEFFREY R ,  SEGEL MICHAEL SARIS ,  GÜRTLER MADS

IPC: C12N5/02 ,  C12N5/00 ,  C12N5/071

CPC classification number: C12N5/0678 ,  C12N5/0606 ,  C12N5/0676 ,  C12N5/0696 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/375 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/40 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/22 ,  C12N2506/45

Abstract: Disclosed herein are methods, compositions, kits, and agents useful for inducing &bgr; cell maturation, and isolated populations of SC-&bgr; cells for use in various applications, such as cell therapy.

Abstract translation: 本发明公开了用于诱导β细胞的方法，组合物，试剂盒和试剂。 细胞成熟和分离的SC- 细胞用于各种应用，如细胞疗法。

公开(公告)号：WO2015002724A2

公开(公告)日：2015-01-08

申请号：PCT/US2014041988

申请日：2014-06-11

Applicant: HARVARD COLLEGE

Inventor： PETERSON QUINN P ,  PAGLIUCA FELICIA J ,  MELTON DOUGLAS A ,  MILLMAN JEFFREY R ,  SEGEL MICHAEL SARIS ,  GÜRTLER MADS

IPC: C12N5/074

CPC classification number: C12N5/0678 ,  C12N5/0606 ,  C12N5/0676 ,  C12N5/0696 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/375 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/40 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/22 ,  C12N2506/45

Abstract: Disclosed herein are methods, compositions, kits, and agents useful for inducing &bgr; cell maturation, and isolated populations of SC-&bgr; cells for use in various applications, such as cell therapy.

公开(公告)号：WO2014172507A1

公开(公告)日：2014-10-23

申请号：PCT/US2014/034435

申请日：2014-04-16

Applicant: MEMORIAL SLOAN-KETTERING CANCER CENTER

Inventor： LORENZ, Studer ,  MILLER, Justine, D.

IPC: C12N5/07

CPC classification number: C12N5/0618 ,  C12N5/0696 ,  C12N2501/40 ,  C12N2501/998 ,  C12N2503/02 ,  C12N2506/1307 ,  G01N33/502 ,  G01N33/5023 ,  G01N33/5058 ,  G01N33/5073

Abstract: Provided are age-modified cells and method for making age modified cells using progerin or a progerin-like protein. The aging and/or maturation process can be accelerated and controlled for young and/or immature cells, such as a somatic cell, a stem cell, a stem cell-derived somatic cell, including an induced pluripotent stem cell-derived cell, by contacting with progerin or a progerin-like protein. Methods described by the present disclosure can produce age- appropriate cells from a somatic cell or a stem cell, such as an old cell and/or a mature cell. Such age- modified cells constitute model systems for the study of late-onset diseases and/or disorders.

Abstract translation: 提供年龄修饰的细胞和使用progerin或progerin样蛋白来制备年龄修饰的细胞的方法。 老化和/或成熟过程可以通过接触来促进和控制幼体和/或未成熟细胞，例如体细胞，干细胞，来自干细胞的体细胞，包括诱导多能干细胞衍生的细胞 与progerin或progerin样蛋白。 通过本公开描述的方法可以从体细胞或干细胞例如老细胞和/或成熟细胞产生适合年龄的细胞。 这种年龄修复的细胞构成了用于研究迟发性疾病和/或病症的模型系统。

公开(公告)号：WO2014158089A1

公开(公告)日：2014-10-02

申请号：PCT/SE2014/050373

申请日：2014-03-27

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： PIJUAN GALITÓ, Sara ,  TAMM, Christoffer ,  ANNERÉN, Cecilia

IPC: C12N5/00 ,  C07K14/81

CPC classification number: C12N5/0696 ,  C12N5/0606 ,  C12N2501/40 ,  C12N2501/998

Abstract: The present invention relates to a method for stem or progenitor cell culture. More precisely, the invention relates to a method for cell culture using one or more IαI (inter-alpha trypsin inhibitor or Inter-alpha inhibitor) protein(s) or part(s) thereof as a component in a cell culture media or a coating on a cell culture surface material. Furthermore the invention relates to a cell culture media and a cell culture coating/matrix provided with one or more II proteins(s) or part(s) thereof.

Abstract translation: 本发明涉及一种干细胞或祖细胞培养方法。 更确切地说，本发明涉及一种使用一种或多种IαI（α间胰蛋白酶抑制剂或Inter-α抑制剂）蛋白或其一部分作为细胞培养基或涂层中的组分的细胞培养方法 在细胞培养表面材料上。 此外，本发明涉及提供有一种或多种I 1 I蛋白或其一部分的细胞培养基和细胞培养物包衣/基质。