公开(公告)号：WO2010085879A1

公开(公告)日：2010-08-05

申请号：PCT/CA2010/000097

申请日：2010-01-27

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE ,  LIN, Wei-Ya ,  YEH , Mary Ya-Ping ,  TSENG, Tzu-Ling ,  CHENG, Ping-Fu ,  HSU, Tsai-Wei ,  LI, Hung-Yi ,  CHEN, Yi-Ting ,  LIN, Yuh-Feng ,  CHEN, Giien-Shuen ,  LI , Yen-Peng

Inventor： LIN, Wei-Ya ,  YEH , Mary Ya-Ping ,  TSENG, Tzu-Ling ,  CHENG, Ping-Fu ,  HSU, Tsai-Wei ,  LI, Hung-Yi ,  CHEN, Yi-Ting ,  LIN, Yuh-Feng ,  CHEN, Giien-Shuen ,  LI , Yen-Peng

IPC: C07K16/38 ,  C07K16/18 ,  C40B30/00 ,  G01N33/53 ,  G01N33/573 ,  G01N33/68 ,  C07K14/81 ,  C07K14/47

CPC classification number: G01N33/6893 ,  G01N2333/4728 ,  G01N2333/52 ,  G01N2333/71 ,  G01N2333/8125 ,  G01N2800/347 ,  G01N2800/52 ,  G01N2800/56

Abstract: Use of urine and serum biomarkers in diagnosing diabetic nephropathy, staging diabetic nephropathy, monitoring diabetic nephropathy progress, and assessing efficacy of diabetic nephropathy treatments. These biomarkers include urine precursor alpha-2-HS-glycoprotein, urine alpha- 1 antitrypsin, urine alpha- 1 acid glycoprotein, urine osteopontin, serum osteopontin, their fragments, and combinations thereof.

Abstract translation: 使用尿液和血清生物标志物诊断糖尿病肾病，分期糖尿病肾病，监测糖尿病肾病进展，评估糖尿病肾病治疗效果。 这些生物标志物包括尿前体α-2-HS-糖蛋白，尿α-1抗胰蛋白酶，尿α-1酸性糖蛋白，尿骨桥蛋白，血清骨桥蛋白，其片段及其组合。

公开(公告)号：WO2010085878A1

公开(公告)日：2010-08-05

申请号：PCT/CA2010/000096

申请日：2010-01-27

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE ,  YEH, Mary Ya-Ping ,  TSENG, Tzu-Ling ,  LU, Ching-Fang ,  LIN, Wei-Ya ,  HSU, Tsai-Wei ,  CHEN, Yi-Ting ,  YANG, Chwei-Shiun

Inventor： YEH, Mary Ya-Ping ,  TSENG, Tzu-Ling ,  LU, Ching-Fang ,  LIN, Wei-Ya ,  HSU, Tsai-Wei ,  CHEN, Yi-Ting ,  YANG, Chwei-Shiun

IPC: C07K16/28 ,  G01N33/493 ,  G01N33/53 ,  G01N33/543 ,  G01N33/566 ,  G01N33/68 ,  C07K14/705 ,  C40B30/00 ,  G01N27/00

CPC classification number: G01N33/6893 ,  C07K14/70503 ,  C07K16/2803 ,  G01N2333/4728 ,  G01N2333/70503 ,  G01N2800/347 ,  G01N2800/52 ,  G01N2800/56

Abstract: This invention relates to the measurement of urine biomarkers levels by mass spectrometry or immune assay for diagnosing nephropathy, monitoring nephropathy progress, assessing efficacy of a nephropathy treatment and assessing renal toxicity of an agent.. These urine biomarkers include leukocyte-associated Ig-like receptor-2, alpha- 1 acid glycoprotein, their fragments, and combinations thereof.

Abstract translation: 本发明涉及通过用于诊断肾病，监测肾病进展，评估肾病治疗的效力和评估药物的肾毒性的质谱法或免疫测定法来测量尿生物标志物水平。这些尿生物标志物包括白细胞相关的Ig样受体 -2，α-1酸性糖蛋白，其片段及其组合。

公开(公告)号：WO2010012086A1

公开(公告)日：2010-02-04

申请号：PCT/CA2009/001055

申请日：2009-07-28

Applicant: GENENEWS CORPORATION ,  LIEW, Choong-Chin ,  MA, Jun ,  GO, Alan, S.

Inventor： LIEW, Choong-Chin ,  MA, Jun ,  GO, Alan, S.

IPC: C12Q1/68 ,  C40B30/00 ,  C40B30/02

CPC classification number: C12Q1/6883 ,  C12Q2600/112 ,  C12Q2600/158 ,  G06F19/18 ,  G06F19/20

Abstract: The application provides methods of determining the severity or monitoring the progression of heart failure in a human test subject by determining the level of RNA encoded by one or more heart failure markers genes in the blood of the test patients compared to controls. A kit comprising primers for genes differentially expressed in heart failure is also taught.

Abstract translation: 该应用提供了通过测定与对照相比，测试患者的血液中一种或多种心力衰竭标记基因编码的RNA水平在人类测试受试者中确定严重程度或监测心力衰竭进展的方法。 还教导了包含用于在心力衰竭中差异表达的基因的引物的试剂盒。

公开(公告)号：WO2008131192A3

公开(公告)日：2010-01-14

申请号：PCT/US2008060774

申请日：2008-04-18

Applicant: UNIV COLUMBIA ,  SCHINDLER CHRISTIAN W ,  SONG LI ,  PERNIS BENVENUTO

Inventor： SCHINDLER CHRISTIAN W ,  SONG LI ,  PERNIS BENVENUTO

IPC: G01N33/53 ,  C40B30/00

CPC classification number: G01N33/92 ,  G01N33/5055 ,  G01N2800/323

Abstract: The present invention discloses that innate immune responses to crystalline cholesterol plays a critical a role in directing the chronic inflammatory state associated with the development of atherosclerotic lesions. Hence, identifying the sensor system(s) that both detects and directs the inflammatory response to crystalline cholesterol will provide an opportunity to develop novel therapeutic agents for the treatment of atherosclerosis.

Abstract translation: 本发明公开了对结晶性胆固醇的先天免疫应答在引导与动脉粥样硬化病变发展相关的慢性炎症状态中起关键作用。 因此，鉴定检测并指导对结晶胆固醇的炎症反应的传感器系统将提供开发用于治疗动脉粥样硬化的新型治疗剂的机会。

公开(公告)号：WO2009124404A1

公开(公告)日：2009-10-15

申请号：PCT/CA2009/000516

申请日：2009-04-09

Applicant: THE UNIVERSITY OF BRITISH COLUMBIA ,  McMANUS, Bruce ,  HOLLANDER, Zsuzsanna ,  MUI, Alice ,  BALSHAW, Robert ,  McMASTER, Robert ,  KEOWN, Paul ,  FREUE, Gabriela, Cohen ,  QASIMI, Pooran ,  NG, Raymond ,  LIN, David ,  WISHART, David ,  BERGMAN, Axel

Inventor： McMANUS, Bruce ,  HOLLANDER, Zsuzsanna ,  MUI, Alice ,  BALSHAW, Robert ,  McMASTER, Robert ,  KEOWN, Paul ,  FREUE, Gabriela, Cohen ,  QASIMI, Pooran ,  NG, Raymond ,  LIN, David ,  WISHART, David ,  BERGMAN, Axel

IPC: C12Q1/68 ,  C40B30/00 ,  C40B30/04 ,  G01N33/483 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68

CPC classification number: G01N33/6893 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N33/505 ,  G01N33/6842 ,  G01N33/6848 ,  G01N2800/245 ,  G01N2800/32

Abstract: The present invention relates to methods of diagnosing acute rejection of a cardiac allograft using genomic expression profiling, proteomic expression profiling, metabolite profiling, or alloreactive T-cell genomic expression profiling,

Abstract translation: 本发明涉及使用基因组表达谱，蛋白质组表达谱，代谢物分析或同种异体反应性T细胞基因组表达谱来诊断心脏同种异体移植物的急性排斥反应的方法，

公开(公告)号：WO2009124403A1

公开(公告)日：2009-10-15

申请号：PCT/CA2009/000514

申请日：2009-04-09

Applicant: THE UNIVERSITY OF BRITISH COLUMBIA ,  McMANUS, Bruce ,  HOLLANDER, Zsuzsanna ,  LIN, David ,  BALSHAW, Robert ,  McMASTER, Robert ,  KEOWN, Paul ,  COHEN FREUE, Gabriela ,  WILSON-McMANUS, Janet ,  NG, Raymond

Inventor： McMANUS, Bruce ,  HOLLANDER, Zsuzsanna ,  LIN, David ,  BALSHAW, Robert ,  McMASTER, Robert ,  KEOWN, Paul ,  COHEN FREUE, Gabriela ,  WILSON-McMANUS, Janet ,  NG, Raymond

IPC: C12Q1/68 ,  C40B30/00 ,  C40B30/04 ,  G01N33/483 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68

CPC classification number: G01N33/6893 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N33/505 ,  G01N33/6842 ,  G01N33/6848 ,  G01N2800/245 ,  G01N2800/32

Abstract: The present invention relates to methods of diagnosing chronic rejection of a cardiac allograft using genomic expression profiling, proteomic expression profiling, or a combination of genomic and proteomic expression profiling.

Abstract translation: 本发明涉及使用基因组表达谱，蛋白质组表达谱或基因组和蛋白质组表达谱的组合来诊断心脏同种异体移植物的慢性排斥反应的方法。

公开(公告)号：WO2009086624A1

公开(公告)日：2009-07-16

申请号：PCT/CA2009/000008

申请日：2009-01-05

Applicant: THE ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING/MCGILL UNIVERSITY ,  PLA, Mateu ,  JUNCKER, David

Inventor： JUNCKER, David

IPC: B81B7/04 ,  B81B1/00 ,  B81C1/00 ,  C40B30/00 ,  C40B30/04 ,  C40B60/12 ,  G01N35/10 ,  C40B50/00

CPC classification number: G01N35/1011 ,  B01J2219/00317 ,  B01J2219/00367 ,  B01J2219/00373 ,  B01J2219/00533 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00644 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00691 ,  B01J2219/00693 ,  B01L3/0248 ,  B01L3/0262 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2200/0642 ,  B01L2300/0819 ,  B01L2300/0887 ,  B01L2400/0688 ,  G01N2035/1037

Abstract: A microfluidic system for fluid transfer to a microarray includes a liquid transfer needle having a fluid conduit therein within which is defined a withholding pressure P1, and a microcompartment defined within the microarray, the microcompartment being configured to generate a capillary pressure P2 therein. The capillary pressure P2 is less than the withholding pressure P1, such that a defined amount of liquid is transferred from the liquid transfer needle into the microcompartment when the liquid transfer needle and the microcompartment are disposed in fluid flow communication. A method of delivering multiple solutions to a plurality of microcompartments in an microarray while avoiding cross-contamination between the solutions is also provided.

Abstract translation: 用于流体转移到微阵列的微流体系统包括其中具有流体导管的液体输送针，其中限定了保持压力P1，以及限定在微阵列内的微型隔室，微型隔室被配置为在其中产生毛细管压力P2。 毛细管压力P2小于滞留压力P1，使得当液体输送针和微型隔室配置成流体连通时，限定量的液体从液体输送针转移到微型隔室中。 还提供了一种将多种解决方案递送到微阵列中的多个微室，同时避免溶液之间的交叉污染的方法。

公开(公告)号：WO2009074882A3

公开(公告)日：2009-06-18

申请号：PCT/IB2008/003869

申请日：2008-10-31

Applicant: LUMINEX MOLECULAR DIAGNOSTICS, INC. ,  GALVAN-GOLDMAN, Barbara ,  ZOLDOWSKI, Agnes ,  BORTOLIN, Susan ,  MERANTE, Franck

Inventor： GALVAN-GOLDMAN, Barbara ,  ZOLDOWSKI, Agnes ,  BORTOLIN, Susan ,  MERANTE, Franck

IPC: C12P19/34 ,  C12Q1/68 ,  C40B30/00 ,  C40B30/04

Abstract: The present invention provides nucleic acid amplification, detection, and genotyping techniques. In one embodiment, the present invention provides a method for amplifying and detecting a target nucleic acid sequence by providing a first primer pair comprising: a first primer comprising a target specific sequence, a tag sequence 5' of the target specific sequence, and a blocker between the target specific sequence and the tag sequence, and a second primer comprising a target specific sequence; providing a reporter attached to either the second primer or to a dNTP; providing a capture complex comprising an anti-tag sequence attached to a solid support; combining the first primer pair, the capture complex, the reporter, and a sample comprising a target nucleic acid sequence under conditions suitable for amplification of the target nucleic acid sequence and hybridization of the amplified target nucleic acid sequence to the capture complex; and detecting the amplified target nucleic acid sequence.

公开(公告)号：WO2009074882A2

公开(公告)日：2009-06-18

申请号：PCT/IB2008003869

申请日：2008-10-31

Applicant: LUMINEX MOLECULAR DIAGNOSTICS ,  GALVAN-GOLDMAN BARBARA ,  ZOLDOWSKI AGNES ,  BORTOLIN SUSAN ,  MERANTE FRANCK

Inventor： GALVAN-GOLDMAN BARBARA ,  ZOLDOWSKI AGNES ,  BORTOLIN SUSAN ,  MERANTE FRANCK

IPC: C12P19/34 ,  C12Q1/68 ,  C40B30/00 ,  C40B30/04

CPC classification number: C12Q1/6858 ,  C12Q2565/514 ,  C12Q2525/186 ,  C12Q2525/161

Abstract: The present invention provides nucleic acid amplification, detection, and genotyping techniques. In one embodiment, the present invention provides a method for amplifying and detecting a target nucleic acid sequence by providing a first primer pair comprising: a first primer comprising a target specific sequence, a tag sequence 5' of the target specific sequence, and a blocker between the target specific sequence and the tag sequence, and a second primer comprising a target specific sequence; providing a reporter attached to either the second primer or to a dNTP; providing a capture complex comprising an anti-tag sequence attached to a solid support; combining the first primer pair, the capture complex, the reporter, and a sample comprising a target nucleic acid sequence under conditions suitable for amplification of the target nucleic acid sequence and hybridization of the amplified target nucleic acid sequence to the capture complex; and detecting the amplified target nucleic acid sequence.

Abstract translation: 本发明提供核酸扩增，检测和基因分型技术。 在一个实施方案中，本发明提供了通过提供第一引物对来扩增和检测靶核酸序列的方法，所述第一引物对包括：包含目标特异性序列的第一引物，靶特异性序列的标签序列5' 在目标特异性序列和标签序列之间，以及包含目标特异性序列的第二引物; 提供连接到第二引物或dNTP的报告基团; 提供包含连接到固体支持物的抗标签序列的捕获复合物; 在适于扩增靶核酸序列的条件和扩增的靶核酸序列与捕获复合物的杂交的条件下，组合第一引物对，捕获复合物，报道子和包含靶核酸序列的样品; 并检测扩增的靶核酸序列。

公开(公告)号：WO2009026416A1

公开(公告)日：2009-02-26

申请号：PCT/US2008/073816

申请日：2008-08-21

Applicant: VDX, LLC ,  MIMMS, Larry, T.

Inventor： MIMMS, Larry, T.

IPC: C12Q1/68 ,  C40B30/00

CPC classification number: C12Q1/6832 ,  C12Q2525/207 ,  C12Q2521/513

Abstract: The present invention concerns methods of using short-controlling RNA sequences (scRNAs) to screen for and identify target nucleic acids that bind to the loop sequences of the scRNAs. The present invention also relates to methods of detecting scRNAs and to diagnostic and therapeutic methods useful in the management of diseases wherein scRNAs or aberrant levels of scRNAs are expressed.

Abstract translation: 本发明涉及使用短控制RNA序列（scRNA）筛选和鉴定结合scRNA的环序列的靶核酸的方法。 本发明还涉及检测scRNAs的方法以及用于治疗其中表达scRNAs或异常水平的scRNAs的疾病的诊断和治疗方法。