公开(公告)号：WO2009079052A2

公开(公告)日：2009-06-25

申请号：PCT/US2008/077092

申请日：2008-09-19

Applicant: APPLIED BIOSYSTEMS INC. ,  YANG, Joon Mo ,  LIU, David, M. ,  CHIANG, Yuh-Min ,  SCHEMBRI, Carol ,  LAU, Aldrich, N. K. ,  ULMANELLA, Umberto ,  BEARD, Nigel, P. ,  SHARIATI, Maryam ,  NURSE, James, C. ,  HAGA, Douwe, D. ,  HARDING, Ian, A. ,  FOCARACCI, Julio, P.

Inventor： YANG, Joon Mo ,  LIU, David, M. ,  CHIANG, Yuh-Min ,  SCHEMBRI, Carol ,  LAU, Aldrich, N. K. ,  ULMANELLA, Umberto ,  BEARD, Nigel, P. ,  SHARIATI, Maryam ,  NURSE, James, C. ,  HAGA, Douwe, D. ,  HARDING, Ian, A. ,  FOCARACCI, Julio, P.

IPC: B01L3/00 ,  C12Q1/68

CPC classification number: B01L3/502707 ,  B01L7/52 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/1805 ,  B01L2300/1861 ,  B01L2400/0677

Abstract: An assay card and devices and methods for isolating chambers on the assay card are described. The assay card comprises a substrate formed of one or more materials, e.g., plastic, having a softening temperature, the substrate defining channels communicating with respective reaction chambers. The assay card may be heated in a region of the channels to at least the softening temperature. The softened plastic may be deformed, e.g., with a tool which may or may not also provide the heat for softening the substrate. In this manner, the plastic of the substrate may be caused to at least partially obstruct the channels, thereby isolating the reaction chambers. The invention also relates to a method of manufacturing a tool device that includes pins for heating and deforming an assay card.

Abstract translation: 描述了用于隔离化验卡上的室的化验卡和装置以及方法。 测定卡包括由具有软化温度的一种或多种材料（例如塑料）形成的基底，基底限定与相应反应室连通的通道。 可以在通道的区域中将测定卡加热到至少软化温度。 软化的塑料可以变形，例如用可以或不可以提供软化基材的热量的工具。 以这种方式，可以使衬底的塑料至少部分地阻塞通道，从而隔离反应室。 本发明还涉及一种制造工具装置的方法，所述工具装置包括用于加热和使检定卡变形的销。

公开(公告)号：WO2009070871A1

公开(公告)日：2009-06-11

申请号：PCT/CA2008/002083

申请日：2008-11-27

Applicant: MDS ANALYTICAL TECHNOLOGIES, A BUSINESS UNIT OF MDS INC. DOING BUSINESS THROUGH ITS SCIEX DIVISION ,  APPLIED BIOSYSTEMS INC. ,  BONNER, Ron, F. ,  BURTON, Lyle, L. ,  LE BLANC, Yves

Inventor： BONNER, Ron, F. ,  BURTON, Lyle, L. ,  LE BLANC, Yves

IPC: H01J49/26 ,  G01N27/00

CPC classification number: H01J49/004 ,  G01N30/7233 ,  G01N30/86 ,  H01J49/0027 ,  Y10T436/24

Abstract: Systems and methods for analyzing compounds in a sample. In one embodiment, the present technology is directed towards a method of analyzing a sample, comprising: emitting ions from the sample; selecting the emitted ions for a designated ion; fragmenting the designated ions; scanning for a plurality of designated ion fragments; determining a designated fragment chromatographic trace for each designated ion fragment; and generating a combined chromatographic trace corresponding to a non-linear combination of a plurality of designated fragment chromatographic traces.

Abstract translation: 用于分析样品中化合物的系统和方法。 在一个实施例中，本技术涉及分析样品的方法，包括：从样品中发射离子; 选择发射的离子指定的离子; 破碎指定的离子; 扫描多个指定的离子碎片; 确定每个指定离子片段的指定片段色谱痕迹; 以及产生对应于多个指定片段色谱痕迹的非线性组合的组合色谱图。

公开(公告)号：WO2009046348A1

公开(公告)日：2009-04-09

申请号：PCT/US2008/078817

申请日：2008-10-03

Applicant: APPLIED BIOSYSTEMS INC. ,  LEHTO, Dennis A. ,  BOEGE, Steven J.

Inventor： LEHTO, Dennis A. ,  BOEGE, Steven J.

IPC: B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  G01N21/05 ,  G01N35/00

CPC classification number: C12Q1/6874 ,  B01J2219/00286 ,  B01J2219/00306 ,  B01J2219/005 ,  B01J2219/00533 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01L3/502 ,  B01L3/50851 ,  B01L7/00 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/043 ,  B01L2300/0822 ,  B01L2300/0877 ,  B01L2300/1805 ,  B01L2300/1811 ,  B01L2300/1894 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1484 ,  G01N21/0332 ,  G01N21/05 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2201/02

Abstract: A device for performing biological sample reactions may include a plurality of flow cells configured to be mounted to a common microscope translation stage, wherein each flow cell is configured to receive at least one sample holder containing biological sample. Each flow cell also may be configured to be selectively placed in an open position for positioning the at least one sample holder into the flow cell and a closed position for reacting biological sample contained in the at least one sample holder. The plurality of flow cells may be configured to be selectively placed in the open position and the closed position independently of each other.

Abstract translation: 用于进行生物样品反应的装置可以包括多个流动池，其构造成安装到普通的显微镜平移台上，其中每个流动池被构造成容纳至少一个含有生物样品的样品架。 每个流动池还可以被配置为选择性地放置在打开位置，用于将至少一个样品保持器定位到流动室中，以及用于使包含在至少一个样品保持器中的生物样品反应的关闭位置。 多个流动池可以被配置为被选择性地彼此相对放置在打开位置和关闭位置。

公开(公告)号：WO1994007133A1

公开(公告)日：1994-03-31

申请号：PCT/US1993009115

申请日：1993-09-22

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： APPLIED BIOSYSTEMS, INC. ,  MENCHEN, Steven, Michael ,  WINNIK, Mitchell, A. ,  JOHNSON, Ben, F.

IPC: G01N27/447

CPC classification number: G01N27/44747

Abstract: A viscous electrophoresis separation medium is disclosed. The medium is formed by a matrix formed by a copolymer composed of hydrophilic polymer segments having selected, substantially uniform segment lengths, and a plurality of hydrophobic polymer segments carried on, and spaced from one another by the hydrophilic polymer segments. Also disclosed is an electrophoresis method which employs the separation medium, and novel copolymers used in forming the medium.

Abstract translation: 公开了一种粘性电泳分离介质。 介质由由具有选定的基本上均匀的段长度的亲水性聚合物链段组成的共聚物和通过亲水性聚合物链段彼此携带并彼此间隔开的多个疏水性聚合物链段形成的基质形成。 还公开了使用分离介质的电泳方法和用于形成培养基的新型共聚物。

公开(公告)号：WO1994005688A1

公开(公告)日：1994-03-17

申请号：PCT/US1993008227

申请日：1993-09-01

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： APPLIED BIOSYSTEMS, INC. ,  MENCHEN, Steven, M. ,  LEE, Linda, G. ,  CONNELL, Charles, R. ,  HERSHEY, N., Davis ,  CHAKERIAN, Vergine ,  WOO, Sam, L. ,  FUNG, Steven

IPC: C07H21/00

CPC classification number: C07H21/00 ,  C07D311/82 ,  C09B11/08 ,  C12Q1/6869 ,  C12Q2563/107

Abstract: Long wavelength, narrow emission bandwidth fluorescein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2',4',5',7'-tetrachloro-4,7-dichloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.

Abstract translation: 提供长波长，窄发射带宽荧光素染料用于检测空间重叠的目标物质。 染料包括4,7-二氯荧光素，特别是2'，4'，5'，7'-四氯-4,7-二氯-5-（和6-）羧基荧光素。 提供了在DNA分析中使用染料的方法和试剂盒。

公开(公告)号：WO1992019367A1

公开(公告)日：1992-11-12

申请号：PCT/US1992003357

申请日：1992-04-23

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： APPLIED BIOSYSTEMS, INC. ,  BUTTRILL, S., E., Jr.

IPC: B01D59/44

CPC classification number: H01J49/40 ,  H01J49/025

Abstract: A time-of-flight mass spectrometer (11-13) in which sample ions are generated from a target (23) and are focussed into an ion beam (14) that is incident onto a detector (13). A barrier (212) that defines an aperture (213) in the path of the ion beam is positioned to block ions having an extra large deviation from an average time-of-flight of the ions, thereby improving resolution. The aperture can be adjusted to adjust a tradeoff between sensitivity and resolution. Alternatively, the position of the aperture or the bias on an einzel lens can be adjusted to control this resolution.

公开(公告)号：WO1992014742A1

公开(公告)日：1992-09-03

申请号：PCT/US1991001008

申请日：1991-02-14

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： APPLIED BIOSYSTEMS, INC. ,  HIRSCHBEIN, Bernard, L.

IPC: C07G03/00

CPC classification number: C07H21/00

Abstract: A method for synthesizing sulfurized oligonucleotide analogs, such as phosphorothioate and phosphorodithioate analogs, is provided that employs a thiuram disulfide as a sulfurizing agent. The method of the invention may be used to sulfurize any phosphorous(III)-containing intermediate. Preferably, the method is practiced on a commercial DNA synthesizer using phosphoramidite and/or phosphorthioamidite intermediates.

Abstract translation: 提供了一种硫代寡核苷酸类似物的合成方法，例如硫代磷酸酯和二硫代磷酸酯类似物，其使用二硫化秋兰姆作为硫化剂。 本发明的方法可用于硫化任何含磷（III）的中间体。 优选地，该方法在商业DNA合成仪上使用亚磷酰胺和/或磷酰胺亚胺酯中间体进行。

公开(公告)号：WO1991007092A1

公开(公告)日：1991-05-30

申请号：PCT/US1990006630

申请日：1990-11-13

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： APPLIED BIOSYSTEMS, INC. ,  ZON, Gerald ,  MATSUKURA, Makoto ,  STEC, Wojciech, J. ,  WILK, Andrzej

IPC: A01N43/04

CPC classification number: A61K31/70 ,  A61K31/66 ,  C07F9/1411 ,  C07H21/04

Abstract: Methods and compositions employing poly(alkyl and alkenyl phosphate)s and/or their thiophosphate derivatives are provided for treating viral infections. Preferably, polymers of the invention 20-30 monomers in length are used alone or in combination to treat HIV infections.

Abstract translation: 提供了使用聚（烷基和链烯基磷酸酯）和/或其硫代磷酸酯衍生物的方法和组合物用于治疗病毒感染。 优选地，本发明的聚合物长度的20-30个单体单独使用或组合使用以治疗HIV感染。

公开(公告)号：WO1991006050A1

公开(公告)日：1991-05-02

申请号：PCT/US1990006000

申请日：1990-10-16

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： APPLIED BIOSYSTEMS, INC. ,  GUIREMAND, Harry, A.

IPC: G06F03/14

CPC classification number: B25J9/1671 ,  G01N35/0092 ,  G05B19/0426 ,  G05B2219/23258 ,  G05B2219/36025 ,  G06F3/0481

Abstract: An automated apparatus (11) is programmed to perform a process by arranging a sequence of first icons (69) on a display (15) in the order of the process, wherein the first icons (69) represent functions of the apparatus (11), and wherein at least one of the first icons (69) provides a visual representation of a function of the apparatus (11). Said at least one of the first icons can be expanded to show second icons (81) that comprise the function of said at least one of the first icons (69), and at least one of the second icons (81) provides a visual representation of a subfunction of the apparatus (11). In a preferred mode, when said at least one of the first icons (69) is expanded, said at least one of the first icons (69) maintains its same sequential relationship on the display (15) to the other of the first icons (69) in the sequence as before it was expanded.

Abstract translation: 自动化装置（11）被编程为通过按照处理顺序排列显示器（15）上的第一图标序列来执行处理，其中第一图标（69）表示装置（11）的功能， ，并且其中所述第一图标（69）中的至少一个提供所述装置（11）的功能的视觉表示。 所述第一图标中的至少一个可以被扩展以显示包括所述至少一个第一图标（69）的功能的第二图标（81），并且所述第二图标（81）中的至少一个提供视觉表示 的装置（11）的子功能。 在优选模式中，当所述第一图标（69）中的至少一个被展开时，所述第一图标（69）中的至少一个在显示器（15）上保持与第一图标（15）中的另一个相同的顺序关系（ 69）按照扩展前的顺序进行。

公开(公告)号：WO1988002004A1

公开(公告)日：1988-03-24

申请号：PCT/US1986001970

申请日：1986-09-20

Applicant: APPLIED BIOSYSTEMS, INC.

Inventor： APPLIED BIOSYSTEMS, INC. ,  FUNG, Steven ,  WOO, Sam, Lee ,  SMITH, Lloyd, M.

IPC: C07F09/22

CPC classification number: C07F9/2416 ,  A61K47/545 ,  A61K47/548 ,  C07F9/2408 ,  C07F9/65842 ,  C07H21/00 ,  C08B37/0021 ,  C08B37/0039

Abstract: The compounds include novel linking agents comprising 2-substituted-3-protected-1,3,2-oxazaphosphacycloalkanes and their phosphoramidite precursors. The compounds of the invention further include conjugates of the above linking agents with oligonucleotides and polymer supports. The compounds of the present invention are useful for linking organic moities, such as fluorescent or chromogenic dyes, to polymer supports and oligonucleotides, particularly single- and double-stranded DNA and RNA fragments.